Preparation of Cholera Enterotoxin Solution
Cholera enterotoxin
Distilled Water
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of supervisor / Neil Hotchin / Assessment Number* / 2027Signature
Assessor / Rachel McMullan / Date of Assessment / 15/5/02
Signature
NotesA School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1 / LOCATION OF THE WORK ACTIVITY / Lab 501 5th Floor School of Biosciences2 / PERSONS WHO MAY BE AT RISK
List names where possible
Neil Hotchin
Rachel McMullan
Vicki Robertson
Dave Radford
Sian Lax
All other laboratory personnel
3 / ACTIVITY ASSESSED / Preparation of cholera enterotoxin solution
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT
max/stored / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Cholera enterotoxin / 1mg / Harmful / Harmful in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin
Distilled Water / None / No significant hazard in the quantities and dilutions used.
5 / INTENDED USE**
Give brief details and attach protocol/instructions
Prevention of keratinocyte differentiation
Add 1.18ml distilled water to 1mg to give a stock concentration of 10-5M. Dilute this to give working solution of 10-10M
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
Risks to health and safety from gross misuse of the above chemicals are discussed in the attatched chemical data sheet. Given the small volumes and dilution for INTENDED use risks to health and safety are negligible provided the stated control measures (see section 8) are adhered to
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Minimal risk from intended use if stated control measures (see section 8) are adhered to.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
a) storage and handling…
Keep container tightly closed in a cool well ventilated area
Do not store below 4oC
Keep away from heat and sources of ignition
Do not ingest or inhale dust
Handle in a fume hood
b) protective measures…
Wear dust respirator, gloves, goggles and a lab coat
c) avoid contact with…
metals and acids
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
"In house" instruction (provided under the supervision of experienced senior personnel) supplemented by university biological and chemical safety courses
10 / MONITORING
Performance of control measures,
Ensure fume hoods/extractor fans are serviced regularly
Personal exposure / Health Surveillance
N/A / N/A
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Dispose of liquid waste by mixing with Virkon bleach solution
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
15/5/07 or if usage/circumstances change significantly
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Only trained personnel should deal with spills etc.
Powder;
Sweep up and place in suitable container, avoid raising dust
Liquid;
Wipe down area with Virkon bleach solution
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Alert all personnel in the vicinity and evacuate the immediate area
All personnel involved in clean up must wear appropriate protective clothing
First aid…
Eyes- Remove any contact lenses Flush eyes with water for at least 15min Seek medical attention
Lungs- Remove to fresh air, if not breathing give artificial respiration. If breathing is difficult give oxygen Seek medical attention
Skin- Flush skin with plenty of water remove any contaminated clothing Cover irritated skin woth an emollient. Seek medical attention
Ingestion- Do not induce vomitting Do not give anything by mouth to an unconcious person Seek medical attention
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
Ventilate area
Contact school safety officer to arrange waste disposal for large spills
Wash down area thoroughly
Inform safety officer of incident
CONTACT Neil Hotchin PHONE 45412
10.10.00
* Prefix T is used for Teaching Assessment Number.
** Please include amount of chemicals used and how.
***Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.