This template serves as a guide with the correct format and content to write a manuscript for submission to the Journal of Visualized Experiments. You may begin writing directly in this document. Guiding notes are provided in grey throughout this document; please remove these notes before submission.

Please strictly follow the guidelines in our Manuscript Instructions for Authors document. Failure to follow these guidelines will delay the editorial and peer review process and extend your publication turnaround time. Should you have any questions about writing your manuscript, please contact your editor, or JoVE’s Editorial Office. Step-by-step instructions to submit can be found here.

Required formatting

·  File Type: Microsoft Word is JoVE’s preferred format for text. All manuscripts must be submitted as a .doc or .docx file

·  Font: 12 pt Arial, Calibri, or Times New Roman throughout the entire document

·  Page margins: 1 inch on all sides

·  Line spacing: single-spaced with a space between each paragraph/bullet point

TITLE: (maximum 150 characters)

AUTHORS: (full affiliations required for all authors, listed in the order of authorship)

Last Name, First Name Middle Initial (if appropriate)

Department

Institution

City, Country

E-mail Address

CORRESPONDING AUTHOR: (required; this is the official corresponding author for publication, but may differ from the author used for submission in the Editorial Manager system)

KEYWORDS: (minimum 6, maximum 12)

Keyword 1, keyword 2, keyword 3, etc.

SHORT ABSTRACT: (10 words minimum, 50 words maximum)

Short abstract text here…

LONG ABSTRACT: (150 words minimum, 300 words maximum)

Long abstract text here…

INTRODUCTION: (150 words minimum, 1500 words maximum)

Introduction text here…

CASE PRESENTATION: (required, if applicable)

Include patient demographics, applicable family/social/medical histories, symptoms and signs, past treatments, and results of physical tests in paragraph form here...

Diagnosis, Assessment, and Plan: (required, if applicable)

Include explanation of initial tests performed, rationale behind the tests, diagnosis reached, symptoms or signs leading to diagnosis, additional tests or procedures used to confirm diagnosis, differential diagnosis, treatment plan, rationale behind planned treatment/procedure, complications and side effects of treatment in paragraph form here...

PROTOCOL: (see Manuscript Instructions for Authors regarding content and length)

Example of protocol content and required number formatting:

2.  Pre-stress Measurements

2.1)  Instruct the participant to wait comfortably for 45 minutes in the waiting room. You may choose to provide the participant with reading material, such as magazines containing emotionally-neutral content, or another emotionally-neutral activity during this period.

2.2)  After the 45-minute waiting period, administer the State Trait Anxiety Inventory to assess pre-stress anxiety.6Alternatively, other instruments that are designed to evaluate state changes in stress, anxiety or relaxation may be administered (e.g. visual analogue scales).

OUTCOME/REPRESENTATIVE RESULTS:

Please add a concise written description of the typical outcome or results following correct execution of this procedure. This section should allude to any submitted figures in complete sentences and paragraph form.

Example outcome/representative results:

The Asthma Intervention Research 2 (AIR2) Trial evaluated the safety and effectiveness of the Alair Bronchial Thermoplasty System. The data from that study was submitted to the FDA and was the basis for the agency’s approval of a novel device for the treatment of asthma. The trial demonstrated that treatment with the Alair System resulted in improved asthma quality of life. Table 1 shows the following clinically significant benefits over time during long-term follow-up: 32 % reduction in asthma attacks, 84 % reduction in emergency room visits for respiratory symptoms, 73 % reduction in hospitalizations for respiratory symptoms, and 66 % reduction in days lost from work/school or other daily activities due to asthma.

Figure Legends: (provide separate files for figures and tables; do not include figures or tables within manuscript document. The default placement for all figures and results tables is below the Representative Results text. Please indicate— via brackets, i.e. [Place Figure 1 here]— if you prefer figure/table placement at another location in the text.)

Figure 1: Figure 1 Title. Figure 1 legend text…

Table 1: Table 1 Title. Table 1 legend text (as needed)…

DISCUSSION: (3-6 paragraphs)

Discussion text here, in paragraph form…

ACKNOWLEDGMENTS: (required)

Acknowledgements text here…

The default text can be “The authors have no acknowledgements.” but it is typical to acknowledge funding sources here.

DISCLOSURES: (required)

Disclosures text here…

The default text can be “The authors have nothing to disclose.”

REFERENCES (10 minimum— use JoVE’s style files to interface with reference software)

1.  Lastname, F.I., Lastname, F.I., Lastname, F.I., Lastname, F.I. Article Title. Source. Volume (issue), FirstPage – LastPage, PII, DOI, (YEAR).

2.  Lastname, F.I., et al. Article Title. Source. Volume (issue), FirstPage – LastPage, PII, DOI, (YEAR).

*Reference style 2 should be used if there are more than 6 authors

Page 3 of 3 rev. October 2013