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Customer Information
If you have an existing account with ProteinCT, just fill in your name and email address or your Account No.
Name of Requestor: / First: Last: Middle: / Title:Account No.:
Name of PI:
Organization:
Phone: / Fax:
Email Address:
Shipping Address
Project Information
Nature of Inquiry / Pricing estimation Quote for ordering For grant application purposeEstimated Project Initiation / Immediately 1-3 months 3-6 months
>6 months Other, please specify
Intended Use / Research Use Only Clinical Applications
Please note that we produce materials for research use only. We can perform developmental work for future clinical applications, but are not GMP-certified.
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Service Specifications
Please fill in project requirement details.
1. General informationTarget: / Protein name: Accession number:
Species: MW:
Protein sequence:
Please indicate mutations/variants as compared to the sequence corresponding to the accession number.
Target properties: / Membrane-bound Secreted Cytosolic Nuclear Periplasm
Protease Other enzymes IgG antibody Toxic to E.coli
Other features to consider, please specify:
Expression system: / Pick an expression system:
Bacteria Yeast Baculovirus/insect cell
Mammalian cell
Transient Transfection Stable cell line expression
Select a mammalian host cell line:
HEK293 CHO Other (Please specify): No preference
HEK293 or its derivatives will be used as default cell line if no preference is selected.
Has the protein been expressed previously?
Yes No
If YES, please indicate the expression host:
Bacteria Yeast Baculovirus/insect cell Mammalian cell
Other (Please specify):
Yield: Solubility: Protocol: Other information:
Please provide your protocol and experience in a separate Word document. The information will be useful for project evaluation and quotation.
Secretion signal sequence:
For insect cell and mammalian cell expression systems / Preferred secretion signal sequence:
Target’s own signal peptide No preference Other (Please specify):
Preferred expression/purification process:
Recommended: Parallel expression evaluation for both secretion with signal peptide and intracellular expression without signal peptide
Secretion expression only (Purification of target protein from the expression medium only)
Purification from the cell lysate when very low purification yield from medium
Expression scale: / Amount of protein: mg or Volume of culture required: liter(s)
Protein modification / Stable isotope labeling
15N 13C
Biotinylation
Intended application(s):
Select all that may apply / High-throughput screening (HTS) Activity assay NMR X-ray crystallography As Antigen for antibody generation in vivo studies
Protein-protein interactions Other (Please specify):
Comments:
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Service Specifications
Please fill in project requirement details.
2. Expression Construct GenerationStarting material: / Recommended: Synthesize and construct the expression vector by ProteinCT:
Gene synthesis with codon optimization (recommended)
Organism for codon optimization:
Enter target gene sequence:
Gene synthesis without codon optimization
Enter target gene sequence:
Subclone from User supplied template DNA - Vector name:
Please attach vector map (e.g. NTI file)
Enter your gene sequence:
User supplied expression ready construct - Expression vector name:
Please attach vector map (e.g. NTI file), indicating your desired cloning site
Enter vector sequence:
Other (Please specify):
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Service Specifications
Please fill in project requirement details.
3. Protein Purification and CharacterizationQC requirements: / Protein purity: %
Protein Concentration: mg/ml
Endotoxin Level:
Buffer: / 1xPBS (default) Other, please specify
Purification method: / ProteinCT will develop the purification method/system for you (default option):
Yes No, use my own purification method/system; Please specify:
Follow user supplied purification protocol:
Yes (Please attach your protocol in a separate Word document: ) No
Fusion tag: / ProteinCT will use the best combination of N-terminal and/or C-terminal tags to enhance expression (default option):
Yes No, use user specified tag(s); Please specify:
Tag removal: / Add a protease cleavage site between the tags and the target protein:
Yes No
If YES, please specify desired protease:
ProteinCT choice (default) Enterokinase PreScission rTev Thrombin
Please note that extra amino acids may be left on target protein
Fusion tag removal:
Yes No Leave small tags (e.g. His) on target protein
Please note that protein yield may be reduced after tag removal
Refolding: / When target protein is expressed insoluble, refolding will be performed to increase yield:
Yes No
Protein characterization: / SDS-PAGE Native PAGE Silver staining
Western blot ELISA HPLC
Endotoxin measurement
N-terminal sequencing MALDI-TOF Others (Please specify):
Special requirements:
e.g. buffer composition, shipping/packaging/handling