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Supplemental material.
Table S1.
Research purpose / Name / Sequence 5’ – 3’Degenerate primer / Ses-233_for
Losel-471_rev
Lo-284_for / AA(AG) TA(CT) GC5(CT)T5 GA(AG) TT(CT) CC5 TGG CA CA(CT) TG(CT)
GAT CTT GGA TGG AGA (AG)TG TAT TTG CTC
TGT GCA CAT CAT AGA GAA CTA CAG CTT
RACE-PCR primer / PsTPS1 for
PsTPS1 rev
PsTPS2 for
PsTPS2 rev
PsTPS3 for
PsTPS3 rev / AGG GAC TCA GAA GTT GCG CAG GTG A
TGT TCG GGA GAG GTT GAC CCA TCA AG
TCA GAG TCA TAT CTG CCG CAG CTG G
CGT CTG GTA GAA TCT GAT CCA TTA AC
GGA TAT AGA GAA GCA GAA TTT CTA CC
GTA TAC ACA TTC CAG AGC TGG GTT T
Nested RACE-PCR primer / PsTPS1nested_3’-for PsTPS1nested_5’-rev
PsTPS2nested_3’-for
PsTPS2nested_5’-rev
PsTPS3nested_3’-for
PsTPS3nested_5’-rev / CCG TCG GAA CTG TAG ATG ACC
GAT GCA CAT CCC TGA GCT AGC
CGT GGA ACT TTA CTT TTG GGC GG
CCG GAG CTA GCG AGG CC
CGT TCG AAC CGG AGT TTT CGA GC
CGC CAT CCA TTC GGA CTC TTG C
Amplification of the full-length coding region / PsTPS1_for
PsTPS1_rev
PsTPS2_for
PsTPS2_rev
PsTPS2-T_for
PsTPS3_for
PsTPS3_rev / CAC CAT GGC TGA GAT TTG TGA ATC AG
TTA TTC GTC CAT AGG TAC TTG CTC
CAC CAT GGC TCT TGT TTC TGT AAC G
TCA CAG CTG CAG TGG CTG GAT AAG
CAC CAT GCTAATGCCAAAGAAATCTGTG
CAC CAT GGC TCA AAT TTC TAT AGG TGC
TCA GAT GGG CAC TTG ATC GAT AAG
qRT-PCR primer / PsTPS1_QRT_for
PsTPS1_QRT_rev
PsTPS2_QRT_for
PsTPS2_QRT_rev
PsTPS3_QRT_ for PsTPS3_QRT_rev
Ubi-QRT-for
Ubi-QRT-rev / TAC GGA ATG CTA GAC GAA CTG
GCG GCC AGT TCA GAC GAA CTG
GGA TCA GAT TCT ACC AGA CG
GTT ATG TCG TCC TTG ACT CG
AGG GTA TAT GCA AGA GTC GC
GTA TAC ACA TTC CAG AGC TGC
GTT GAT TTT TGC TGG CAA
CAC CTC TCA GAC GAA GTA
Table S2.