PHYTOCHEMICAL, ANTIOXIDANT AND

ANTIMICROBIAL STUDIES ON

LEAVES OF

BARLERIA PRIONITIS LINN

SYNOPSIS FOR

M.PHARM DISSERTATION

SUBMITTED TO

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES

BY

POOJA GUPTA

Department of Pharmacognosy

THE OXFORDCOLLEGE OF PHARMACY

BANGALORE-78

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES

KARNATAKA, BANGALORE

ANNEXURE –11

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION
1. / Name of the candidate and Address / POOJA GUPTA
The OxfordCollege of Pharmacy,
J.P.Nagar, 1st Phase,
Bangalore-560078.
PERMANENT ADDRESS:
D/o Krishna Kumar Gupta,
# 31, Chowki Shumshari,
Etawah (U.P)
2. / Name of the Institution / The OxfordCollege of Pharmacy,
J.P.Nagar, 1st Phase,
Bangalore-560078.
3. / Course of study and subject / M.Pharm, Pharmacognosy
4. / Date of Admission / 23 June 2008
5. / Title of the Topic / PHYTOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES ON LEAVES of

BARLERIA PRIONITIS LINN

6. Brief Resume of the intended work:

6.1 – Need for the study:

Barleria prionitis (Acanthaceae), also known as Jhinti is a small, branched, spiny shrub, found growing throughout the hotter parts of India. It is commonly grown as hedge plant in gardens.

The leaves and flowering tops are rich in soluble potassium salts and used as diuretic. Fresh Juice of bark is diaphoretic and expectorant. Roots are used as febrifuge and as a paste to releive toothache1.

The Barleria prionitis plant contain two new iridoids identified as 5,6 β–epoxy-7 β–hydroxy-3 β-methyl 1 β-D-rhamnosidal iridoid and 4-carbomethoxy-7 β, 8 α– dihydroxy-8- β - methyl 1 β -D-glucopyranosidal iridoid.

The root contains a new acylated flavonoid glycoside, identifiedas luteolin–7-0-

(2”-0-p-coumaroyl)-β -D-glycopyranoside2.

As per the folklore uses it has been known that the leaf part is used in the treatment of various wounds and leaf juice is used the lacerated soles of feet. And the phytochemical studies reported of certain flavonoids from leaves which support the possible antioxidant activity and antimicrobial activity.

Therefore in present study it is proposed to carry out isolation of the chemical constituents fromthe leaves of Barleria prionitis and in vitroantioxidant and

antimicrobial activity of theextract and/or isolated compounds.

6.2 – Review of Literature

  • Thefreshflowersof Barleriaprionitiswerereported to contain

flavone glycoside 5,6,4’- trihydroxy-7-0-neohesperidosylflavone3.

  • Two new iridoid compounds barlerinand acetyl barlerin has been

reported to be present in leaf and stem parts of Barleria prionitis4.

  • The aqueous extract of leaves ofBarleria prionitisshowed moderate

diuretic activityat a dose of 2.5ml/100g in rats. They were more

potent as diuretic than urea but less potent than potassium acetate.

The diuretic activity of leaves was determined with reference to

urea asstandard in terms of T/U5.(urinary excretion of test drug).

  • Antifertility study for root extract of Barleriaprionitis in male albino

rats with special reference to testicular cell population dynamicshas

been reported6.

  • A 3:1 mixture of two new iridoid glycoside 6-0-trans-p- coumaryal-

8-0-acetylshanzhiside methyl ester and its cis isomer were isolated

from Barleria prionitis, has shown to have in vitro-activity

against respiratory syncytial virus7.

  • TAF an active fraction from the plant Barleria prionitis

showed antiflammatory8.

6.3 Objective of the Study:

The objective of the present studies is to collect the drug i.e.,(leaf of Barleria prionitis from Tumkur and Bangalore and further authenticated from CCRAS Bangalore and used to carry out the extraction (Maceration/soxhlet method), Phytochemical screening, isolation of the phytoconstituents by column chromatography and further the characterization of isolated compounds. Studies of extracted and/or isolated for antimicrobial and in-vitro antioxidant activity.

7. Materials and Methods:

7.1 a) Source of Data:

Chemical abstracts, Search on Medline and other Journals from The Oxford College of Pharmacy, RGUHS-Digital library.

b) Place of work: The Oxford College of Pharmacy, Bangalore.

7.2 Method of Collection of Data:

  • Collection-Authenticated samples of leaf of Barleria prionitis from various places of Tumkur and Bangalore.
  • Extraction – Extracts of drugs will be prepared using different solvents by maceration/soxhlet.
  • Phytochemical Screening-Theextract would be subjected for preliminary

phytochemical screeningforvarious phytochemical groups.

  • Isolation – Isolation of extracted phytoconstituents will be done using colum chromatrography/separation method.
  • Identification and characterization – The isolated compounds will be identified using analytical methods like UV, IR and NMR spectroscopy.
  • Antioxidant activity – The extracts and/or isolated compounds will be studied for in-vitro antioxidant activity of DPPH9/superorxide radical method10.
  • Antimicrobial activity – The extracts and/or isolated compounds will be studied for an antimicrobial activity by diffusion disc method11.

7.3 – Does the study require any investigations or inventions to be conducted

on patients or other humans or animals?

If so, please describe briefly.

-Not applicable -

7.4 – Has ethical clearance been obtained from your institution in case of 7.3?

-Not applicable -

8. Bibliography:

1- Sharma PC, YelneMB, Dennis TJ, Joshi A, Prabhune YS, Borker GB et

al. Data base on medicinal plants used in Ayurveda central council for

research in ayurveda & siddha New Delhi 2001;(l) :378-379.

2- Gupta AK, Sharma Madhu,Tandon Neeraj, Reviews on Indian Medicinal

Plants. Indian Council of Medicinal Research New Delhi 2004; (4): 71-72.

3-Rastogi Ram P, Mehorta BN, Sinha Sharadha, Srivastava Mukta, Bhushan

Bela. Compendium of Indian Medicinal Plants NewDelhi 2001; (3): 90.

4- Rastogi Ram P, Mehortra BN, Sinha Sharadha , Pant Pushpa, Seth

Renu. Compendium of Indian Medicinal Plants New Delhi 2001; (2): 94.

5- Gupta AK, Sharma Madhu,Tandon Neeraj, Reviews on Indian Medicinal

Plants. Indian Council of Medicinal Research New Delhi 2004; (4): 76.

6- Gupta RS, Kumar P, Dixit VP, Dobhal MP. Antifertility study of the root

extract of Barleria prionitis in male albino rats.Journal Ethnopharmacology,

2000 May; 70(2) :111-117.

7- Ray AB,Sharma BK, Singh UP. Medicinal properties of plants, Antifungal,

Antibacterial and Antimicrobial activation International Book distributing

Co 2004; 95.

8- Singh B, Babu S, Gupta DK,Chandan BK, Kaula. Anti-inflammatory

activity of TAF on active fraction from the plant Barleria prionits

Journal of Ethnopharmacology 2008 April; 85 (2):187-193(7).

9- Vani T, Rajani M, Sarkar S, Shishoo C, Antioxidant properties of the

ayurvedic formulation Triphala and its constituents. Int. J.Pharmacog

1997; 35(5) ;317-7.

10- Beauchamp C, Fridovich I, Superoxide dismutiase. Improved assays

and assays applicable to acrylamide gels. Anal Biochem 1971; 44; 276-7

11- Srafulalam, Madiar Rehman. Atimicrobial activity of Barleria prioritis

on some pathogenic bacteria Bangladesh Journal of Scientific and

Industrial Research 2008 ; 43:3.

9. / Signature of candidate
10. / Remarks of the Guide / Recommended
11. / 11.1 Name and Designation of Guide / Mrs.Usha Gavani
Asst. Professor
Department of Pharmacognosy
11.2 Signature
11.3 Co-guide
11.4 Signature
11.5 Head of the Department / Dr.Padmaa M.Paarakh
Professor and Head Department of Pharmacognosy
11.6 Signature
12. / 12.1 Remarks of the Chairman and Principal / Forwarded to the University for scrutiny
12.2 Signature / Dr.Padmaa M.Paarakh
Principal
The OxfordCollegeof Pharmacy,
J.P.Nagar, 1st Phase,Bangalore-78