“PHYTOCHEMICAL AND BIOLOGICAL STUDIES FROM THE ROOT OF THESPESIA POPULNEA LINN”

DISSERTATION PROTOCOL

Submitted to the

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA

BY

D.CHANDRAMOHAN

M.Pharm Part-1

DEPARTMAENT OF PHARMACEUTICAL CHEMISTRY

UNDER THE GUIDANCE OF

Mr. M GURUMURTHY, M.Pharm

ASSISTANT PROFESSOR

Department of pharmaceutical chemistry

Dr H.L.T College of pharmacy

Kengal, channapatna -571502

2008

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES, KARNATAKA

BANGALORE

ANNEXURE II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1. / Name of the Candidate and
Address( in Block Letters) / D.CHANDRAMOHAN
M. Pharm, Part-I
DEPARTMENT OF PHARMACEUTICAL CHEMISTRY
Dr. H. L. T. COLLEGE OF PHARMACY
KENGAL, CHANNAPATNA-571502
BANGALORE (RURAL), KARNATAKA
2. / Name of the Institution / Dr. H.L.T. College of Pharmacy
Kengal, Channapattana-571 502
3. / Course of Study and Subject / Master of Pharmacy
Pharmaceutical Chemistry
4. / Date of Admission to the Course / 25-5-2008
5. / Title of the Topic / “PHYTOCHEMICAL AND BIOLOGICAL STUDIES FROM THE ROOT OF THESPESIA POPULNEA LINN”

6) BRIEF RESUME OF THE INTENDED WORK:

6.1) NEED FOR THE STUDY :

Milo’s scientific name is thespesia populnea1,2 and it is also know as Portia tree . thespesia is a genus of Malvaceae with basically tropical and subtropical world wide distribution3. Probably it originated in India, but is a common plant of costal strands across old world tropics.

Especially in south India thespesia populnea is commonly named as medicinal plant and found to possess useful medicinal properties3,4 such as antifertility , anti-inflammatory, antioxidant , purgative and hepto protective5 activity etc and also the bark , leaves and flowers are useful in cutaneous infections such as scabes , psoriasis , eczema , ringworms , guinea worm , anti-inflammatory for poultice as a folk medicine etc.

Literature survey revealed that the phytochemical screening of thespesia populnea was carried out by isolating various phytoconstituents from different parts of the plant using various standard procedures6 for extraction using the solvents by increasing polarity

Petroleum ether, Ethyl acetate , chloroform , methanol etc.

So for there is no clear report on the phytochemical and biological studies from the root of Thespesia populnea. Hence this work has been taken up for phytochemical investigation and screening for biological activity on root of thespesia populnea .

6.2.) REVIEW OF LITERATURE

The review of various published work related to subject revealed the following

  1. Fryxell, P.A. 1966, Australian Plants: Thespesia in Australia Sept.1966 vol.3 pp 366-367.
  1. Morton, J.F. 1966, Australian Plants: Seaside mahoe (Thespesia populnea Soland.) Sept.1966 vol.3 pp 367-369.
  1. Uses of Plants. Bishop Museum Press, Honolulu.. Allen, J.A. 2002. Cordia subcordata. Tropical Tree Seed Manual pp. 18–419. Agriculture Handbook 721. USDA Forest Service, Washington, DC. In: Vozzo,J.A. (ed.)
  1. Fosberg, F.R , and M.H. Sachet. 1972. Thespesia populnea (L.)Solander ex Correa and Thespesia populneoides (Roxburgh) Kosteletsky (Malvaceae). Smithsonian Contributions to Botany 7: 1–13.
  1. Shirwaikarkumar A, Krishnan AV, Sreenivasan KK. Chemical investigation and antihepatotoxic activity of Thespesia populnea . Int J Pharmacog. 1995; 33: 305-10.
  1. Bevans,N. Alford,M. Guanches,F. Aregullin,M. Rodriguez,E. 2001 "Preliminary Phytochemical Study of Two Caribbean Malvaceae Used in the Treatment of Conjunctivitis" J USAIR Issue 2, 20-24 , 2001.
  1. Eavens WC and treese pharmacognosy.London ,ballitere, tindall , 1996,pp 388-43.
  1. In view of above facts. we aimed work on phytochemistry and biological studies on root of thespesia populnea and screening them for biological activity.

6.3.) MAIN OBJECTIVES OF THE STUDY :

The present work is an attempt :

  1. To investigate the phytochemical constituents and biological activity on root of Thespesia populnea linn.
  1. extraction of root powder by using the solvents like water , Petroleum ether, Ethyl- acetate , chloroform , methanol and purification of extract through chromatographic studies , identification of phytoconstituents by chemical test etc
  1. Microbial assay are carried out on root extract by cup-plate method using gram +ve and gram −ve bacteria.

7. MATERIALS AND METHODS:

7.1) SOURCE OF DATA

The preliminary data required for the experimental study is obtained from .

  1. CD-Rom search available at national center for scientific information (NCSI), Indian institute of Sciences, Bangalore,
  2. Journals ,
  3. Analytical chemistry Books,
  4. Library,
  5. Relevant Books,
  6. Internet Sources,
  7. Scientific abstracts.

7.2) METHODS OF COLLECTION OF DATA:

The preliminary data required for the experimental study is obtained from –

  1. CD-Rom search available at National Center of Scientific Information (NCSI), Indian institute of Sciences, Bangalore, Dr. H.L.T. College of Pharmacy Library, Scientific abstracts, Journals, Internet sources and Relevant Books.
  2. The data will be collected by Laboratory investigation and Recording the data.

7.3)Does the Study require any investigation to be conducted Patients/Humans/animal?

If so, please describe briefly.

YES , Activity of phytochemical constituents will be carried out on in vivo model

7.4) Has ethical Clearance been obtained from your institution in case of 7.3 ?

The study is cleared from Ethical committee of Institution

601/02c/CPCSEA

Copy enclosed

8. LIST OF REFERENCES:

  1. Fryxell, P.A. 1966, Australian Plants: Thespesia in Australia Sept.1966 vol.3 pp 366-367.
  1. Morton, J.F. 1966, Australian Plants: Seaside mahoe (Thespesia populnea Soland.) Sept.1966 vol.3 pp 367-369.
  1. Uses of Plants. Bishop Museum Press, Honolulu.. Allen, J.A. 2002. Cordia subcordata. Tropical Tree Seed Manual pp. 18–419. Agriculture Handbook 721. USDA Forest Service, Washington, DC. In: Vozzo,J.A. (ed.)
  1. Fosberg, F.R , and M.H. Sachet. 1972. Thespesia populnea (L.)Solander ex Correa and Thespesia populneoides (Roxburgh) Kosteletsky (Malvaceae). Smithsonian Contributions to Botany 7: 1–13.
  1. Shirwaikarkumar A, Krishnan AV, Sreenivasan KK. Chemical investigation and antihepatotoxic activity of Thespesia populnea . Int J Pharmacog. 1995; 33: 305-10.
  1. Bevans,N. Alford,M. Guanches,F. Aregullin,M. Rodriguez,E. 2001 "Preliminary Phytochemical Study of Two Caribbean Malvaceae Used in the Treatment of Conjunctivitis" J USAIR Issue 2, 20-24 , 2001.
  1. Eavens WC and treese pharmacognosy.London ,ballitere, tindall , 1996,pp 388-43.
  1. On the Web:
  1. A description of the use of milo in old Hawai‘i: <
  1. For a detailed description of milo propagation, see the Native
  1. Plants Network: <
  1. For a general article on milo, see Australian Native Hibiscus:
  1. <
  1. Species Profiles for Pacific Island Agroforestry ( 1
  1. For information on milo as an invasive species in new areas.
  1. Examples of wood carving using milo in the Cook Islands:<
  1. Propagation tips: < hawnprop/the-popu.htm>.
  1. University of Hawaii College of Tropical Agriculture andHuman Resources Landscape Series <
  1. How to Prune Trees, USDA Forest Service: <

9. / Signature of the Candidate
10. / Remarks of the Guide / Topic selected for Dissertation work is satisfactory. This can be carried out in our Laboratory.
11. / Name and Designation of
( In Block Letters)
11.1Guide
11.2Signature / Mr.M. GURUMURTHY. M.Pharm.
ASSISTANT PROFESSOR,
DEPT OF PHARMACEUTICALCHEMISTRY
Dr. H.L.T. COLLEGE OF PHARMACY,
KENGAL, CHANNAPATNA- 571 502
BANGALORE (RURAL), KARNATAKA.
11.3Co-Guide (If any)
11.4Signature
11.5Head of the
Department
11.6Signature / Mr. M. GURUMURTHY, M. Pharm
ASSISTANT PROFESSOR
DEPT.OF PHARMACEUTICAL CHEMISTRY
Dr. H.L.T. COLLEGE OF PHARMACY,
KENGAL, CHANNAPATTANA-571 502,
Dist: Ramanagar.
12. / 12.1 Remarks of the
Chairman and
Principal
12.2 Signature / Topic selected for Dissertation work is satisfactory. This can be carried out in our Laboratory.

From

D.CHANDRAMOHAN M.Pharm Part-1

DEPARTMENT OF PHARMACEUTICAL CHEMISTRY

Dr. H.L.T. COLLEGE OF PHARMACY

KENGAL, CHANNAPATNA,

BANGALORE (RURAL)-571 502.

To

THE REGISTRAR (EVALUATION),

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA BANGALORE

4TH “T” BLOCK, JAYANAGAR,

BANGALORE-560 041.

(THROUGH PROPER CHANNEL)

SUB : SUBMISSION OF SYNOPSIS OF DISSERTATION.

RESPECTED SIR,

HERE WITH, I AM SUBMITTING SYNOPSIS OF DISSERTATION WORK “PHYTOCHEMICAL AND BIOLOGICAL STUDIES FROM THE ROOT OF THESPESIA POPULNEA LINN” FOR REGISTRATION IN M.PHARM. (PHARMACEUTICAL CHEMISTRY) OF RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE.

KINDLY ACCEPT THE SAME AND OBLIGE.

THANKING YOU,

YOUR’S FAITHFULLY,

Place : Channapatna. (D.CHANDRAMOHAN)

Date :

GUIDE :

MR. M. GURUMURTHY

ASSISTANT PROFEESSOR & HEAD OF PRINCIPAL

DEPT. OF PHARMACEUTICAL CHEMISTRY DR. H.L.T. COLLEGE OF PHARMACY

DR. H.L.T. COLLEGE OF PHARMACY KENGAL, CHANNAPATNA,

CHANNAPATNA. BANGALORE(R)