Planta-2011-01-0008 revision

The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice

Chang-Quan ZHANG, Yong XU, Yan LU, Heng-Xiu YU, Ming-HongGU, Qiao-Quan LIU *

Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College, Yangzhou University, Jiangsu 225009, China.

*Corresponding author, e-mail:

RcWRKY ----MDTNSNNSGDPSRLDSDS------GSAASVGGARYKLMSPAKLPISRSPCI 46

PtWRKY ----MDNNTSRS-EVSDCGDPT------RPESGGGGARYKLMSPAKLPISRSACI 45

VvWRKY ------MSPAKLPISRSPCL 15

CsWRKY17 ----MDPTDSDLPDPSNASS------GAKYKLLSPAKLPISRSPCI 37

AtWRKY20 ----MNPQANDRKEFQGDCSATGDLTAK--HDSAGGNGGGGARYKLMSPAKLPISRSTDI 55

TaWRKY17 MADSPNPSSGDLPSPAGSSPDKPYPADRRVAALAG----AGARYKAMSPARLPISREPCL 57

HvWRKY46 ------MSPARLPISRESCL 15

OsWRKY78 MADSPNPSSGDHPAGVGGSPEKQPPVDRRVAALAAGAAGAGARYKAMSPARLPISREPCL 61

ZaWRKY MADSPNPSSGDLPAGAGCSPEKPVLADRRVSALAG----AGARYKAMSPARLPISREPCL 57

:***:*****.. :

RcWRKY TIPPGLSPTSFLESPVLLSNVKAEPSPTTGSFAKPPTGHGSLGSNPYSATA---VPSNAF 103

PtWRKY TIPPGLSPTSFLESPVLLSNVKAEPSPTTGTFTKPRTALGSLSSTPYSATT---VSSTAC 102

VvWRKY TIPSGLSPSSFLDSPVLLSNMKVEPSPTTGSFAKPQMIHGSVGSSMFSSTSN-CSNSNTF 74

CsWRKY17 TIPPGLSPTSFLDSP------VEPSPTTGSFTKLPMAHDSSGSAIYPMTSMAFSNTNAS 90

AtWRKY20 TIPPGLSPTSFLESPVFISNIKPEPSPTTGSLFKPRPVHISASSSSYTGRG---FHQNTF 112

TaWRKY17 TIPAGFSPSALLDSPVLLTNFKVEPSPTTGSLSMAAIMHKSAHPDMP-SPRDK-SIR-AH 114

HvWRKY46 TIPAGFSPSALLDSPVLLTNFKVEPSPTTGSLGMAAILHKSAHPDMLPSPRDK-SVRNAH 74

OsWRKY78 TIPAGFSPSALLESPVLLTNFKVEPSPTTGTLSMAAIMNKSANPDILPSPRDK-TSGSTH 120

ZaWRKY TIPTGFSPGALLESPVLLNNFKVEPSPTTGTLSMAANMNKSTHPDILPSPRDN-SADSGQ 116

***.*:** ::*:** *******:: * .

RcWRKY GERTSSCFEFRPHPRSN------LVPADVHHRITEQSVQVQGHCQNQSLASSPRVK 153

PtWRKY GERKSDYFEFRPYARSN------MVSADINHQRSTQCAQVQSQCHSQSFASPPLVK 152

VvWRKY DERKSGSFEFKPHTVSNSASGLFLVGSPVSADLNHQQVKPFIPVQAQHPDLSLMPSPTVK 134

CsWRKY17 DEGRSNYFEFKPYVGPN------MVPADLSHRKGEQFSEVQGQP--QPFTAPPMTK 138

AtWRKY20 TEQKSSEFEFRPPASN------MVYAELGKIRSEPPVHFQGQGHGSSHS--PSSI 159

TaWRKY17 EDGGSRDFEFKPHLNSS------SQSVAPAMSDLKKHEHSMQNQSMNPSSSSSNMVN 165

HvWRKY46 EDRGSRDFEFKPHLNSS------SQSLAPAMSDLKKHEHSMQNQSMNPSSSSSNMVN 125

OsWRKY78 EDGGSRDFEFKPHLNSS------SQSTASAINDPKKHETSMKNESLNTALSSDDMMI 171

ZaWRKY EDGGSREFEFKPHLNS------QSSATAVNNQNHHDIPMQNHGSNHASPSSNLMT 165

: * ***:* * .: . .

RcWRKY SEMAVSSNEFSLSAPLP--SSGTSAPG-EVESDELNQIGVSNNGLQASQTDHK---GGSG 207

PtWRKY GEMEVSTNELSLSASLHMVTSVASAPA-EVDSDELNQTGLSSSGLQASQSDHR---AGTA 208

VvWRKY SEMMAPSNELSLCSPGHMVTSLESAPA-EVDSDELNQQGHPNNGVQASQSDQKGIGPSAV 193

CsWRKY17 MEISVMSNDLSRSTQMDTHTVASGVSVPEANGDEINHSLNTNSRVQAPQSDPKGSGIPVV 198

AtWRKY20 SDAAGSSSELSRPTPPCQMTPTSSDIP--AGSD------QEESIQTSQNDSR----GST 206

TaWRKY17 ENRPPCSRESSLTVNVS-APNQPVGMVGLTDSMPAEVGTSEPQQMNSSDNAMQEPQSENV 224

HvWRKY46 ENRPPCSRESSLTVNVS-AQNQPVGMVGLTDSMPAEVGTSEPQQMNSSDNAMQEPQSENV 184

OsWRKY78 DNIPLCSRESTLAVNISSAPSQLVGMVGLTDSSPAEVGTSELHQMNSSGNAMQESQPESV 231

ZaWRKY ENKPLCSRESSHTANVSSAPNQPVSIVCPSDNMPAEVGTSEMHLINSSENAAQETQTENV 225

: : : : . :::. . :

RcWRKY ISMPSDDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITEIIYKGTHD 267

PtWRKY PSMSSDDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITEIIYKGTHD 268

VvWRKY VERSSEDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERAHDGQIVEIIYKGTHD 253

CsWRKY17 SDRLSDDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKGTHD 258

AtWRKY20 PSILADDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKGTHD 266

TaWRKY17 ADKSADDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERAVDGLITEVVYKGRHN 284

HvWRKY46 ADKSADDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERAVDGLITEVVYKGRHN 244

OsWRKY78 AEKSAEDGYNWRKYGQKHVKGSENPRSYYKCTHPNCDVKKLLERSLDGQITEVVYKGRHN 291

ZaWRKY AEKSAEDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERSLDGQITEVVYKGHHN 285

. ::***************** ************:****:**: ** *.:::*** *:

RcWRKY HPKPQPSRRYSSGAVLSMQEDRSDKILSLPGRDDKTSIAYGQVSHTIDPNGTPELSPVTA 327

PtWRKY HPKPQPSRRYASGSVLSMQEDRFDKSSSLPNQGDKSPGAYGQVPHAIEPNGALELS-TGA 327

VvWRKY HPKPQPSRRYASGAILPVQEERPDKVSSLIGRDDKSPSIYGQMAHNIDPNGTPELSPVAA 313

CsWRKY17 HPKPQPSRRYSASASMNVQEDGTDKPSSLPGQDDRSCSMYAQTMHTIEPNGTTDPS-MPA 317

AtWRKY20HPKPQPGRRNSGG--MAAQEERLDKYPSSTGRDEKGSGVY-NLSNPNEQTGNPEVPPISA 323

TaWRKY17 HPKPQPNRRLAGGAVPLNQGE--ERYDDAAAADDKSSNALSNLANAVNSPGMVEPVPVSV 342

HvWRKY46 HPKPQPNRRLAGGAVPSNQGE--ERYDGASAADDKSSNALSNLANPVHSPGMVEPVPASV 302

OsWRKY78HPKPQPNRRLSAGAVPPIQGE--ERYDGVATTDDKSSNVLSILGNAVHTAGMIEPVPGSA 349

ZaWRKY HPKPQPNRRLAAGAVPSSQAE--ERYDGVAPIEDKPSNIYSNLCNQAHSAGMVDNVPGPA 343

******.** :.. * : :: . :: : . * : .

RcWRKY NDDSIEGAE------DDDDPFSKRRKMDTGGFEVTPVVKPIREPRVVVQTLSEVD 376

PtWRKY NDDTGEGAE------DDDDPFSKRRRLDAGGFDVTPVVKPIREPRVVVQTLSEVD 376

VvWRKY NDDVVEGAIL------DEVDEDDPLSKRRKMEIGGIDVTPVVKPIREPRVVVQTLSEVD 366

CsWRKY17 NDRITEGAGTTLPCKNHDEVDDDDIYLKRRKMELGGFDVCPMVKPIREPRVVVQTLSEVD 377

AtWRKY20 SDDGGEAAASN---RNKDEPDDDDPFSKRRRMEG-AMEITPLVKPIREPRVVVQTLSEVD 379

TaWRKY17 SDDDIDAGGGR-SYPGDDGTEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSEVD 401

HvWRKY46 SDDDIDAGGGR-PYPGDDATEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSEVD 361

OsWRKY78 SDDDNDAGGGR-PYPGDDAVEDDDLESKRRKMESAAIDAALMGKPNREPRVVVQTVSEVD 408

ZaWRKY SDDDVDAGGGR-PYPGDDSNDDDDLDSKRRKMESAGIDAALMGKPNREPRVVVQTVSEVD 402

.* :.. :::* ***::: .:: : ** *********:****

RcWRKY ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNAGCPVRKHVERASHDPKAVITTYEGKHNHD 436

PtWRKY ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNAGCPVRKHVERASHDPKAVITTYEGKHNHD 436

VvWRKY ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNAGCPVRKHVERASHDPKAVITTYEGKHNHD 426

CsWRKY17 ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHD 437

AtWRKY20 ILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDPKAVITTYEGKHDHD 439

TaWRKY17 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVVTTYEGEHNHE 461

HvWRKY46 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHNHE 421

OsWRKY78 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTNTGCPVRKHVERASHDPKSVITTYEGKHNHE 468

ZaWRKY ILDDGYRWRKYGQKVVKGNPNPRSYYKCTHTGCPVRKHVERASHDPKSVITTYEGKHNHE 462

****************:************ ****************:*:*****:*:*:

RcWRKY VPMARTSSHDTTGPTAVNGASRIRS------EESETISLDL 471

PtWRKY VPTARTNSHDMAGPSAVNGPSRIRP------DENETISLDL 471

VvWRKY VPTARTNSHDAAGQVALNGMPMIRS------EENDTISLDL 461

CsWRKY17 VPTAKTSSHDVTGPSTIP-SSRYRL------EESDTISLDL 471

AtWRKY20 VPTSKSSSNHEIQP------RFRP------DETDTISLNL 467

TaWRKY17 VPAARNAIHEMSAPPMKNVVHQINSNMPSSIGGMMRACEVRNFSNQYSQAAETDNVSLDL 521

HvWRKY46 VPAARNATHEMSAPPMKNVVHQINSNMPSSIGGMMRACEARNYTNQYSQAAETDTVSLDL 481

OsWRKY78 VPASRNASHEMSTPPMKPVVHPINSNMQG-LGGMMRACEPRTFPNQYSQAAESDTISLDL 527

ZaWRKY VPASRNASHEMSAAPMKPVVHPINSSMPG-FGGMMRACDARAFNNQYSQAAESDTISLDL 521

** ::. :. . *.:.:**:*

RcWRKY GVGISSTTDNRSNDQQQALHSELSQSQNQASGSSFRIVPRA---TIAPYYGVLNGGMNQY 528

PtWRKY GVGISSTTENQSSDQQQALHAELIKHENQASGSSFRVVHAT---PITAYYGVLNGGMNQY 528

VvWRKY GVGINSASENRPNVQHQTLHAELVLTQTRTNNSNFQAVQAT---PVSRYYGVLNNGMDLY 518

CsWRKY17 GVGIGTGGENRSNEYRQALHSQLVENRAPSGNFNFEVVQEN---SAPTYFGVLN------522

AtWRKY20 GVGISSDGPNHASNEHQHQNQQLVN-QTHPNGVNFRFVHAS---PMSSYYASLNSGMNQY 523

TaWRKY17 GVGISPNHSDATN-QMQSTGPDQMQYQMQPMASMYSNMRHP-SMAMPTVQGNSAGR-MYG 578

HvWRKY46 GVGISPNHSDATN-QMQSSGPDQMQYQMQTMGSMYGNMRHPSSMAAPAVQGNSAAR-MYG 539

OsWRKY78 GVGISPNHSDATN-QLQSSVSDQMQYQMQPMGSVYSNMGLP-AMAMPTMAGNAASN-IYG 584

ZaWRKY GVGISPNHSDATN-QMQPSVPEPMQYQMRHMAPVYGSMGLP-GMPVPAIPGNVVASSIYG 579

****.. : .. * : . : : . . .

RcWRKY GSRQNPNEGRSVEIPPLSHSSYPYPQNVGRLLTGP 563

PtWRKY GSRQIPGESRSIEIPP-----YPYPQNMGRLLTGP 558

VvWRKY GFRENLGESRSFETPPLNHSSNPCPQNMGRILMGP 553

CsWRKY17 ------RSVIN------527

AtWRKY20 GQRETKNETQNGDISSLNNSSYPYPPNMGRVQSGP 558

TaWRKY17 SREDKGSEGFTFRATPMDHSANLCYSGAGNLVMGP 613

HvWRKY46 SREEKGNEGFTFRATPMDHSANLCYSSAGNLVMGP 574

OsWRKY78 SREEKPSEGFTFKATPMDHSANLCYSTAGNLVMGP 619

ZaWRKY SREEKGNEGFTFKAAPLDRSANLCYSSAGNLVMGP 614

ESM_Fig. S3. Alignment of OsWRKY78 with related WRKY proteins from different species. Red and green shadings indicate conserved WRKY amino acid residues and zinc-binding motif. The putative NLS were underlined. The accession numbers are OsWRKY78 (Q5HZ67, Oryza sativa subsp. Japonica), AtWRKY20 (Q93WV0, Arabidopsis thaliana),TaWRKY17 (B3GAU7, Triticum aestivum),ZaWRKY (B3GAU7, Zea mays), HvWRKY46 (Q6VWJ6, Hordeum vulgare), VvWRKY (D7T877,Vitis vinifera), RcWRKY (B9SSS9,Ricinus communis), CsWRKY17 (E7CEW6, Cucumis sativus), and PtWRKY (B9GJX1, Populus trichocarpa).