Planta-2011-01-0008 revision
The WRKY transcription factor OsWRKY78 regulates stem elongation and seed development in rice
Chang-Quan ZHANG, Yong XU, Yan LU, Heng-Xiu YU, Ming-HongGU, Qiao-Quan LIU *
Key Laboratory of Plant Functional Genomics of the Ministry of Education, Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College, Yangzhou University, Jiangsu 225009, China.
*Corresponding author, e-mail:
RcWRKY ----MDTNSNNSGDPSRLDSDS------GSAASVGGARYKLMSPAKLPISRSPCI 46
PtWRKY ----MDNNTSRS-EVSDCGDPT------RPESGGGGARYKLMSPAKLPISRSACI 45
VvWRKY ------MSPAKLPISRSPCL 15
CsWRKY17 ----MDPTDSDLPDPSNASS------GAKYKLLSPAKLPISRSPCI 37
AtWRKY20 ----MNPQANDRKEFQGDCSATGDLTAK--HDSAGGNGGGGARYKLMSPAKLPISRSTDI 55
TaWRKY17 MADSPNPSSGDLPSPAGSSPDKPYPADRRVAALAG----AGARYKAMSPARLPISREPCL 57
HvWRKY46 ------MSPARLPISRESCL 15
OsWRKY78 MADSPNPSSGDHPAGVGGSPEKQPPVDRRVAALAAGAAGAGARYKAMSPARLPISREPCL 61
ZaWRKY MADSPNPSSGDLPAGAGCSPEKPVLADRRVSALAG----AGARYKAMSPARLPISREPCL 57
:***:*****.. :
RcWRKY TIPPGLSPTSFLESPVLLSNVKAEPSPTTGSFAKPPTGHGSLGSNPYSATA---VPSNAF 103
PtWRKY TIPPGLSPTSFLESPVLLSNVKAEPSPTTGTFTKPRTALGSLSSTPYSATT---VSSTAC 102
VvWRKY TIPSGLSPSSFLDSPVLLSNMKVEPSPTTGSFAKPQMIHGSVGSSMFSSTSN-CSNSNTF 74
CsWRKY17 TIPPGLSPTSFLDSP------VEPSPTTGSFTKLPMAHDSSGSAIYPMTSMAFSNTNAS 90
AtWRKY20 TIPPGLSPTSFLESPVFISNIKPEPSPTTGSLFKPRPVHISASSSSYTGRG---FHQNTF 112
TaWRKY17 TIPAGFSPSALLDSPVLLTNFKVEPSPTTGSLSMAAIMHKSAHPDMP-SPRDK-SIR-AH 114
HvWRKY46 TIPAGFSPSALLDSPVLLTNFKVEPSPTTGSLGMAAILHKSAHPDMLPSPRDK-SVRNAH 74
OsWRKY78 TIPAGFSPSALLESPVLLTNFKVEPSPTTGTLSMAAIMNKSANPDILPSPRDK-TSGSTH 120
ZaWRKY TIPTGFSPGALLESPVLLNNFKVEPSPTTGTLSMAANMNKSTHPDILPSPRDN-SADSGQ 116
***.*:** ::*:** *******:: * .
RcWRKY GERTSSCFEFRPHPRSN------LVPADVHHRITEQSVQVQGHCQNQSLASSPRVK 153
PtWRKY GERKSDYFEFRPYARSN------MVSADINHQRSTQCAQVQSQCHSQSFASPPLVK 152
VvWRKY DERKSGSFEFKPHTVSNSASGLFLVGSPVSADLNHQQVKPFIPVQAQHPDLSLMPSPTVK 134
CsWRKY17 DEGRSNYFEFKPYVGPN------MVPADLSHRKGEQFSEVQGQP--QPFTAPPMTK 138
AtWRKY20 TEQKSSEFEFRPPASN------MVYAELGKIRSEPPVHFQGQGHGSSHS--PSSI 159
TaWRKY17 EDGGSRDFEFKPHLNSS------SQSVAPAMSDLKKHEHSMQNQSMNPSSSSSNMVN 165
HvWRKY46 EDRGSRDFEFKPHLNSS------SQSLAPAMSDLKKHEHSMQNQSMNPSSSSSNMVN 125
OsWRKY78 EDGGSRDFEFKPHLNSS------SQSTASAINDPKKHETSMKNESLNTALSSDDMMI 171
ZaWRKY EDGGSREFEFKPHLNS------QSSATAVNNQNHHDIPMQNHGSNHASPSSNLMT 165
: * ***:* * .: . .
RcWRKY SEMAVSSNEFSLSAPLP--SSGTSAPG-EVESDELNQIGVSNNGLQASQTDHK---GGSG 207
PtWRKY GEMEVSTNELSLSASLHMVTSVASAPA-EVDSDELNQTGLSSSGLQASQSDHR---AGTA 208
VvWRKY SEMMAPSNELSLCSPGHMVTSLESAPA-EVDSDELNQQGHPNNGVQASQSDQKGIGPSAV 193
CsWRKY17 MEISVMSNDLSRSTQMDTHTVASGVSVPEANGDEINHSLNTNSRVQAPQSDPKGSGIPVV 198
AtWRKY20 SDAAGSSSELSRPTPPCQMTPTSSDIP--AGSD------QEESIQTSQNDSR----GST 206
TaWRKY17 ENRPPCSRESSLTVNVS-APNQPVGMVGLTDSMPAEVGTSEPQQMNSSDNAMQEPQSENV 224
HvWRKY46 ENRPPCSRESSLTVNVS-AQNQPVGMVGLTDSMPAEVGTSEPQQMNSSDNAMQEPQSENV 184
OsWRKY78 DNIPLCSRESTLAVNISSAPSQLVGMVGLTDSSPAEVGTSELHQMNSSGNAMQESQPESV 231
ZaWRKY ENKPLCSRESSHTANVSSAPNQPVSIVCPSDNMPAEVGTSEMHLINSSENAAQETQTENV 225
: : : : . :::. . :
RcWRKY ISMPSDDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITEIIYKGTHD 267
PtWRKY PSMSSDDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITEIIYKGTHD 268
VvWRKY VERSSEDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERAHDGQIVEIIYKGTHD 253
CsWRKY17 SDRLSDDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKGTHD 258
AtWRKY20 PSILADDGYNWRKYGQKHVKGSEFPRSYYKCTHPNCEVKKLFERSHDGQITDIIYKGTHD 266
TaWRKY17 ADKSADDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERAVDGLITEVVYKGRHN 284
HvWRKY46 ADKSADDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERAVDGLITEVVYKGRHN 244
OsWRKY78 AEKSAEDGYNWRKYGQKHVKGSENPRSYYKCTHPNCDVKKLLERSLDGQITEVVYKGRHN 291
ZaWRKY AEKSAEDGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERSLDGQITEVVYKGHHN 285
. ::***************** ************:****:**: ** *.:::*** *:
RcWRKY HPKPQPSRRYSSGAVLSMQEDRSDKILSLPGRDDKTSIAYGQVSHTIDPNGTPELSPVTA 327
PtWRKY HPKPQPSRRYASGSVLSMQEDRFDKSSSLPNQGDKSPGAYGQVPHAIEPNGALELS-TGA 327
VvWRKY HPKPQPSRRYASGAILPVQEERPDKVSSLIGRDDKSPSIYGQMAHNIDPNGTPELSPVAA 313
CsWRKY17 HPKPQPSRRYSASASMNVQEDGTDKPSSLPGQDDRSCSMYAQTMHTIEPNGTTDPS-MPA 317
AtWRKY20HPKPQPGRRNSGG--MAAQEERLDKYPSSTGRDEKGSGVY-NLSNPNEQTGNPEVPPISA 323
TaWRKY17 HPKPQPNRRLAGGAVPLNQGE--ERYDDAAAADDKSSNALSNLANAVNSPGMVEPVPVSV 342
HvWRKY46 HPKPQPNRRLAGGAVPSNQGE--ERYDGASAADDKSSNALSNLANPVHSPGMVEPVPASV 302
OsWRKY78HPKPQPNRRLSAGAVPPIQGE--ERYDGVATTDDKSSNVLSILGNAVHTAGMIEPVPGSA 349
ZaWRKY HPKPQPNRRLAAGAVPSSQAE--ERYDGVAPIEDKPSNIYSNLCNQAHSAGMVDNVPGPA 343
******.** :.. * : :: . :: : . * : .
RcWRKY NDDSIEGAE------DDDDPFSKRRKMDTGGFEVTPVVKPIREPRVVVQTLSEVD 376
PtWRKY NDDTGEGAE------DDDDPFSKRRRLDAGGFDVTPVVKPIREPRVVVQTLSEVD 376
VvWRKY NDDVVEGAIL------DEVDEDDPLSKRRKMEIGGIDVTPVVKPIREPRVVVQTLSEVD 366
CsWRKY17 NDRITEGAGTTLPCKNHDEVDDDDIYLKRRKMELGGFDVCPMVKPIREPRVVVQTLSEVD 377
AtWRKY20 SDDGGEAAASN---RNKDEPDDDDPFSKRRRMEG-AMEITPLVKPIREPRVVVQTLSEVD 379
TaWRKY17 SDDDIDAGGGR-SYPGDDGTEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSEVD 401
HvWRKY46 SDDDIDAGGGR-PYPGDDATEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSEVD 361
OsWRKY78 SDDDNDAGGGR-PYPGDDAVEDDDLESKRRKMESAAIDAALMGKPNREPRVVVQTVSEVD 408
ZaWRKY SDDDVDAGGGR-PYPGDDSNDDDDLDSKRRKMESAGIDAALMGKPNREPRVVVQTVSEVD 402
.* :.. :::* ***::: .:: : ** *********:****
RcWRKY ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNAGCPVRKHVERASHDPKAVITTYEGKHNHD 436
PtWRKY ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNAGCPVRKHVERASHDPKAVITTYEGKHNHD 436
VvWRKY ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNAGCPVRKHVERASHDPKAVITTYEGKHNHD 426
CsWRKY17 ILDDGYRWRKYGQKVVRGNPNPRSYYKCTNVGCPVRKHVERASHDPKAVITTYEGKHNHD 437
AtWRKY20 ILDDGYRWRKYGQKVVRGNPNPRSYYKCTAHGCPVRKHVERASHDPKAVITTYEGKHDHD 439
TaWRKY17 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVVTTYEGEHNHE 461
HvWRKY46 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHNHE 421
OsWRKY78 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTNTGCPVRKHVERASHDPKSVITTYEGKHNHE 468
ZaWRKY ILDDGYRWRKYGQKVVKGNPNPRSYYKCTHTGCPVRKHVERASHDPKSVITTYEGKHNHE 462
****************:************ ****************:*:*****:*:*:
RcWRKY VPMARTSSHDTTGPTAVNGASRIRS------EESETISLDL 471
PtWRKY VPTARTNSHDMAGPSAVNGPSRIRP------DENETISLDL 471
VvWRKY VPTARTNSHDAAGQVALNGMPMIRS------EENDTISLDL 461
CsWRKY17 VPTAKTSSHDVTGPSTIP-SSRYRL------EESDTISLDL 471
AtWRKY20 VPTSKSSSNHEIQP------RFRP------DETDTISLNL 467
TaWRKY17 VPAARNAIHEMSAPPMKNVVHQINSNMPSSIGGMMRACEVRNFSNQYSQAAETDNVSLDL 521
HvWRKY46 VPAARNATHEMSAPPMKNVVHQINSNMPSSIGGMMRACEARNYTNQYSQAAETDTVSLDL 481
OsWRKY78 VPASRNASHEMSTPPMKPVVHPINSNMQG-LGGMMRACEPRTFPNQYSQAAESDTISLDL 527
ZaWRKY VPASRNASHEMSAAPMKPVVHPINSSMPG-FGGMMRACDARAFNNQYSQAAESDTISLDL 521
** ::. :. . *.:.:**:*
RcWRKY GVGISSTTDNRSNDQQQALHSELSQSQNQASGSSFRIVPRA---TIAPYYGVLNGGMNQY 528
PtWRKY GVGISSTTENQSSDQQQALHAELIKHENQASGSSFRVVHAT---PITAYYGVLNGGMNQY 528
VvWRKY GVGINSASENRPNVQHQTLHAELVLTQTRTNNSNFQAVQAT---PVSRYYGVLNNGMDLY 518
CsWRKY17 GVGIGTGGENRSNEYRQALHSQLVENRAPSGNFNFEVVQEN---SAPTYFGVLN------522
AtWRKY20 GVGISSDGPNHASNEHQHQNQQLVN-QTHPNGVNFRFVHAS---PMSSYYASLNSGMNQY 523
TaWRKY17 GVGISPNHSDATN-QMQSTGPDQMQYQMQPMASMYSNMRHP-SMAMPTVQGNSAGR-MYG 578
HvWRKY46 GVGISPNHSDATN-QMQSSGPDQMQYQMQTMGSMYGNMRHPSSMAAPAVQGNSAAR-MYG 539
OsWRKY78 GVGISPNHSDATN-QLQSSVSDQMQYQMQPMGSVYSNMGLP-AMAMPTMAGNAASN-IYG 584
ZaWRKY GVGISPNHSDATN-QMQPSVPEPMQYQMRHMAPVYGSMGLP-GMPVPAIPGNVVASSIYG 579
****.. : .. * : . : : . . .
RcWRKY GSRQNPNEGRSVEIPPLSHSSYPYPQNVGRLLTGP 563
PtWRKY GSRQIPGESRSIEIPP-----YPYPQNMGRLLTGP 558
VvWRKY GFRENLGESRSFETPPLNHSSNPCPQNMGRILMGP 553
CsWRKY17 ------RSVIN------527
AtWRKY20 GQRETKNETQNGDISSLNNSSYPYPPNMGRVQSGP 558
TaWRKY17 SREDKGSEGFTFRATPMDHSANLCYSGAGNLVMGP 613
HvWRKY46 SREEKGNEGFTFRATPMDHSANLCYSSAGNLVMGP 574
OsWRKY78 SREEKPSEGFTFKATPMDHSANLCYSTAGNLVMGP 619
ZaWRKY SREEKGNEGFTFKAAPLDRSANLCYSSAGNLVMGP 614
ESM_Fig. S3. Alignment of OsWRKY78 with related WRKY proteins from different species. Red and green shadings indicate conserved WRKY amino acid residues and zinc-binding motif. The putative NLS were underlined. The accession numbers are OsWRKY78 (Q5HZ67, Oryza sativa subsp. Japonica), AtWRKY20 (Q93WV0, Arabidopsis thaliana),TaWRKY17 (B3GAU7, Triticum aestivum),ZaWRKY (B3GAU7, Zea mays), HvWRKY46 (Q6VWJ6, Hordeum vulgare), VvWRKY (D7T877,Vitis vinifera), RcWRKY (B9SSS9,Ricinus communis), CsWRKY17 (E7CEW6, Cucumis sativus), and PtWRKY (B9GJX1, Populus trichocarpa).