Online Resource Figure S1. Framework map used in QTL analyses of phenotypic data for germination at 25°C and 35°C as well as germination at 20°C in darkness following exposure to far-red light.
Online Resource Figure S1 continued
Online Resource Figure S1 continued
Online Resource Table S1. LE designations and primer pairs for candidate genes annotated to the lettuce genetic map.
Marker / Forward / ReverseLE4010 / AAGAACCGAGAATCGGCTG / CAATTCCGATACTTCTGCTTCA
LE4015 / AGTCGACTTTGAGGACGATG / TATGTCACATCCTTTGGCCC
LE4019 / TCGATGATCTCCGAGCTACTG / ATAAAGTTGCACCAGCGTGA
LE4021 / GAGGATCCAAACGAACCAAA / CTTGAAATGAGGTGCCAACA
LE4022 / TGAATGCCAATCTATCTATCGTG / TTCTGTCTAGCAAATTTGAGCTTG
LE4025 / CTTCATCATCAACGTCGGG / TCACCCTATGCTCTACACTCTCA
LE4034 / AATCTCTGACATGAAATCGGC / TGCCCTCTTCCAAGATTATCA
LE4051 / GAAGTTGCTGTGGAAGGCTC / CTTTCCCTGATCTCTGCAATG
LE4121 / TGATCGGACGGTCATAGTTG / ATTTCGTGAATTTGGGAGCA
LE4128 / GGACCTTGGATTCTCCATCA / TCAACTCCTCAAGAGCCACC
LE4129 / TCCCTCGTTTTCAGTCTTGG / TGAATTAGCAGCAGCATAAGTTGT
LE4130 / GTTTGAAGCGGTTCATCTGG / CCCCCACCTATTGAGTTTCA
LE4131 / CCAACAAAAATTCCCACTCG / TCAACCGGAGCAAAATTACC
LE4132 / GAATCGGAATGGACGAAAAC / CGGCTTGTGATCGTTTGATA
LE4133 / GCCCGTGATGAAGAAGGATA / TAGTCATCCGCTTCCAGTCC
LE4134 / GACCCAATGAAACTGTTGCC / CGCCTCTACCACCTCCTCTT
LE4201 / GATGTGGGTTGGGTCGAATA / GGCATGGCAGATAGTGGTTT
LE4209 / CAGATGCTGTCACCAAATTCA / TAGCCGTGCATTCTTAAAACC
LE4220 / CATGGGTCTTGCAGCTCATA / ATTTGATGGTGGGCCATAGA
LE4245 / GCACAACCTGCTTTAGATTCAA / GCTTATTCCCAAAAGCACCA
LE4250 / TAAACCAAACCGGCATTAGC / GTCCATCATGGTCTGGAGGT
LE4251 / CTCGTAAACTCTGGCCCAAC / TCCTTGTGATCCATCCCATT
1
Online Resource Table S2. Primer sequences for EST markers used in MAS by linkage group.
LG / Marker / Forward / Reverse1 / LE3035 / TCGAGTCGCTTGAAAGACAA / GCCCATAAATCATGCCATTT
1 / LE1112 / TGCAGCAGATAAAGCTGTGG / TCGGGTAAGAAATGGCAAAC
1 / LE0381 / TTGTTGTGTCGGAGAGTGGA / TTGAAATATCCAAGCAATAAGGG
2 / LE3172 / GATTTCTTTCGCCCATCTGA / GGCTAACGATCTTGAAACTGC
2 / LE0032 / CCTGTACTTTGGGCATCGTT / CGCATTTTGAAACAGAGCAA
2 / LE0371 / TCCTCAATTCGGTAGGGTTG / GGCAGGTACCCTTTGCTAGG
2 / LE3033 / AGAGGGCATTGCAAGAAAGA / CATCATGGTCGTGGAGACAG
2 / LE0358 / GCAGGAAGCAAAAGCAAGAA / AGGAAGGGAGGGCATACTGT
3 / LE0146 / GACCACCACCACCCACTAAC / TACGAATCGGTTTCCGAAGA
3 / LE0348 / CCCAGGTTCCGTAATACAGC / GTCGGATGAGCTTGGAGAAT
3 / LE1032 / GATGTAAAACGACGGCCAGTA / GGGTAGAATCCATGGGTGTG
3 / LE0435 / TTCCTTTGAAGAAAGCTAGGG / CCGTTGCGGATACTGAAAGT
4 / LE0498 / TGGATTTCCAAGTTATGTGCTG / GCAGCAACTGAATCACAAAGA
4 / LE0139 / AACGCCGGAATATCACTGTT / TCCTCCTCCTCCTCCTCAAC
4 / LE0333 / GGACCGGGTTTTTAAGTCGT / TTTCTCTGTATATATGCAATCTCCT
4 / LE3257 / TGCCATCGATACAAATGGAG / GGTTGCAGAAGATCATGGGT
5 / LE0483 / AGCACACAACAAATTGTCAGG / TCCCCCACTAATCATTGATACA
5 / LE0352 / GTGGGTAGGAGGAGGAGGAG / ATTCCGATGTGGGAAATCAA
5 / LE0369 / GAGAAATGGGCAGGGTCAT / CAACCCAGATTTTGACTTTCG
6 / LE3059 / AGCATCACCCTGGTCAAATC / GATTGGGGTTCCTTGGAAGT
6 / LE1169 / GATGAGCCGACGAATCATTT / CAACGTGTCACAGCCTGATT
6 / LE0196 / AACTCGCTCACAGGTCCAAT / CGAAGCTGAACAAGCGATTT
6 / LE0237 / GGTTATGCAATCGGGAAGAA / ATACGCCAAACCTTGAGCTG
6 / LE0316 / GGTGGTGGCTGAGTTGTTG / CCCACTAAGCGGAGATTTCA
6 / LE0398 / CAGCCGCCTCTATCATCTTC / AGAACGTCGCCGTTTTTACC
7 / LE9019 / GGCTCAGAAGCGTTGGATTGATT / CTCACCAGAATCAACAGCAGCAAG
8 / LE9042 / GCCAGATCTTGTAAACACCATGTC / CCAGCTGAAGCCATCAACAGCAAC
8 / LE0460 / CCGGATCTGGTTTGTGAGTT / ACTTTCCGACCTCACTTTGC
8 / LE0017 / AACTCGTTTTCGGTGATTGG / GCACTTTCTTTGGCTGAAGG
9 / LE0360 / GGGCTCGTTTATGGTGTTTT / AGGCTGCATTCACCACATACT
1
Online Resource Table S3. Genes mapped within ~10 cM of Htg6.1 (LG6) in a Lactuca sativa cv. Salinas x L. serriola UC96US23 RIL population.
Contig1 or GenBank ID / Gene2 / Description / Position (cM)CLSS2403.b1_F01.ab1 / Expressed protein with methyltransferase domain / 32.4
CLSM3815.b1_N18.ab1 / PDR11 / ABC transporter family protein, ATPase / 33.0
CLV_S1_Contig1777 / EMB2733 / RNA helicase, RNA splicing / 36.5
CLSM1367.b1_M06.ab1 / EMB2733 / RNA helicase, RNA splicing / 37.3
QGG10M24.yg.ab1 / MGD8 / Protein transporter / 38.8
CLSM19714.b1_D09.ab1 / KCBP / Protein kinase activity / 40.2
CLSS4494.b2_K20.ab1 / Expressed protein, unknown function / 41.7
CLSS4105.b1_A20.ab1 / COAB / Coenzyme A biosynthetic process / 41.8
CLS_S3_Contig6272 / Electron carrier/iron-sulfur cluster binding (chloroplast) / 41.8
CLSS3345.b1_A22.ab1 / Acid phosphatase class B family protein / 42.1
CLVZ4523.b1_F04.ab1 / Self-incompatibility protein-related / 42.9
CLS_S3_Contig6125
(LE0196) / Leucine-rich repeat trans-membrane protein kinase / 43.0
CLS_S3_Contig10956 / Disease resistance response protein-related / 43.1
GB_84579411 / LsNCED4
(AtNCED6) / 9-cis-epoxycarotenoid dioxygenase 4 / 43.5
CLRY9048.b1_O06.ab1 / REV7 / Translesion synthesis polymerase zeta subunit (REV7)-related protein / 43.7
CLS_S3_Contig8988 / PKT3 / Acetyl-CoA C-acyltransferase (3-ketoacyl-CoA thiolase) / 44.3
CLS_S3_Contig7464 / Dihydroxyacid dehydratase-related protein / 44.5
CLS_S3_Contig2643 / Expressed protein, oxidoreductase / 45.5
QGB6H05.yg.ab1 / PAP15 / Purple acid phosphatase-related protein / 47.1
CLPY4291.b1_E17.ab1 / No homologs found / 47.5
CLS_S3_Contig3266 / Cysteine proteinase inhibitor B (cystatin B)-related protein / 49.0
CLS_S3_Contig641 / Splicing factor, mRNA processing / 49.3
CLSM7752.b1_O17.ab1 / No homologs found / 49.5
CLSM18950.b1_L09.ab1 / Aldo/keto reductase family protein / 52.1
1Contig names refer to the Compositae Genome Project database ( LsNCED4 was not present as an EST in the database; its GenBank number is shown.
2Highest ranked homolog in Arabidopsis, if named.