Online Resource 1: Tables describing the responses of ant species and non-ant arthropod families to invasion by Nylanderiafulva.

Table A:Responses of ant species to invasion by N.fulva. Abundance of ants from pitfall traps at two sites partially invaded by N.fulvain central Texas.Sampling stations were either in habitat uninvaded by N. fulva(N. fulva-scarce) or at least 100 m behind the invasion front (N. fulva-abundant).

Site1 / Species2 / Biogeo. Category3 / Head Width4 (mm) / Total Stations Present / Prevalence5 - N. fulva-scarce(%) / Prevalence5 - N. fulva-abundant (%) / Abund.6 - N. fulva-scarce (workers per trap) / Abund.6 - N. fulva-abundant (workers per trap) / P value7
HDS / B. patagonicus / Global / 0.37 / 6 / 14 / 10 / 0.1 / 0.1 / 0.71
HDS / C. mauritanica / Global / 0.35 / 30 / 41 / 68 / 2.3 / 4.0 / 0.03
HDS / C. cf.venustula8 / Global / 0.46 / 6 / 5 / 16 / 0.1 / 0.5 / 0.18
HDS / C. rimosus / Global / 0.71 / 14 / 23 / 29 / 0.4 / 1.0 / 0.41
HDS / F. pallidefulva / Regional / 1.30 / 6 / 23 / 3 / 0.7 / 0.03 / 0.03
HDS / H. opaciceps / Global / 0.63 / 12 / 45 / 6 / 1.7 / 0.6 / 0.001
HDS / H. punctatissima / Global / 0.54 / 1 / 5 / 0 / 0.1 / 0 / NA
HDS / L. elongata / Regional / 1.01 / 2 / 5 / 3 / 0.1 / 0.03 / NA
HDS / N. cf. faisonensis9 / Regional / 0.59 / 1 / 0 / 3 / 0 / 0.03 / NA
HDS / N.fulva / Invasive / 0.60 / 34 / 14 / 100 / 0.3 / 907.4 / 0.0001
HDS / N. terricola / Regional / 0.49 / 11 / 50 / 0 / 1.2 / 0.03 / 0.0001
HDS / P. moerens / Global / 0.85 / 17 / 45 / 23 / 2.6 / 0.3 / 0.03
HDS / S. invicta / Invasive / 1.37 / 28 / 100 / 19 / 39.1 / 0.5 / 0.0001
HDS / S. silvestrii / Global / 0.42 / 3 / 5 / 6 / 0.1 / 0.1 / NA
MDS / B. patagonicus / Global / 0.37 / 8 / 16 / 15 / 0.2 / 0.6 / 0.95
MDS / F. pruinosus / Global / 0.58 / 25 / 37 / 55 / 6.6 / 5.0 / 0.07
MDS / H. opaciceps / Global / 0.63 / 1 / 5 / 0 / 0.1 / 0 / NA
MDS / M. minimum / Regional / 0.43 / 2 / 5 / 3 / 0.1 / 0.4 / NA
MDS / N. fulva / Invasive / 0.60 / 41 / 42 / 100 / 5.6 / 138.1 / 0.0001
MDS / N. terricola / Regional / 0.49 / 6 / 32 / 0 / 0.5 / 0 / 0.0007
MDS / P. moerens / Global / 0.85 / 7 / 26 / 6 / 0.6 / 0.1 / 0.03
MDS / P. barbatus / Regional / 2.30 / 2 / 11 / 0 / 0.3 / 0 / NA
MDS / P. membranifera / Global / 0.45 / 3 / 0 / 9 / 0 / 0.1 / NA
MDS / S. invicta / Invasive / 1.37 / 45 / 100 / 79 / 25.1 / 14.6 / 0.004
MDS / S. texana / Regional / 0.34 / 5 / 11 / 9 / 0.2 / 0.2 / NA
MDS / S. silvestrii / Global / 0.42 / 1 / 0 / 3 / 0 / 0.03 / NA

1HDS: high density N. nr. pubens site. MDS: moderate density N. fulva site. Sites are Iowa Colony, TX (HDS), and El Campo, TX (MDS).

2Brachymyrmex patagonicus (Mayr), Cardiocondyla mauritanica(Forel), Cardiocondyla venustula (Wheeler), Cyphomyrmex rimosus (Spinola), Formica pallidefulva (Latreille), Forelius pruinosus (Roger), Hypoponera opaciceps (Mayr), Hypoponera punctatissima (Roger), Leptogenys elongata (Buckley), Monomorium minimum(Buckley), Nylanderia faisonensis (Forel), Nylanderia fulva (Mayr),Nylanderia terricola (Buckley), Pheidole moerens (Wheeler), Pogonomyrmex barbatus (Smith), Pyramica membranifera (Emery), Solenopsis invicta(Buren),Solenopsis texana (Emery), Strumigenys silvestrii (Emery).

3 Based upon their published global distributions species are categorized as: Regional: occurring only in the Neartic;Global: occurring in multiple biogeographic provinces but not documented to negatively impact the natural systems they colonize; or Invasive: species exotic to the Neartic that are documented to negatively impact systems they colonize.

4 The average head width of the 3 largest workers encountered in the pitfall samples.

5 Fraction of sites present.

6 Average number of workers per trap across all traps within that habitat type.

7 Results of a Wilcoxon Rank Sums test comparing mean abundance in the two habitats. Only species present in at least 6 traps tested. NA: insufficiently abundantto test.

8Worker keys out to Cardiocondyla venustula and matchesC. venustula morphologically. Identification is left provisional because C. venustula is not reported to occur regionally. All Cardiocondyla in Texas are exotic species, so determination does not affect biogeographic category.

9A single worker was encountered in an area of dense N. fulva. As it most closely resembles N. faisonensis, it is designated as regional. Changing this designation does not affect significance for any reported results.

Table B: Non-ant, arthropods at a site with a high density N. fulvapopulation located near Iowa Colony, Texas. Data are from pitfall traps at stations located either in habitat uninvaded by N. fulva(N. fulva-scarce) or at least 100 m behind the invasion front (N. fulva-abundant). Family or Superfamily groups are presented when species within them are present at 10 or more stations. Otherwise taxa are grouped at Order or higher level. Taxonomic groups of Order level or higher underlined.

Group / Prev.1 (%) / Number of Species / Abund. N. fulva-scarce2 / Abund. N. fulva-abundant2 / % Change Average Abund.3 / P value4
Acari / 20 / 3 / 0.73 / 0.08 / -89 / 0.04
Araneae5 / 89 / 18 / 4.84 / 2.40 / -50 / 0.0008
Lycosidae / 45 / 9 / 0.92 / 0.47 / -49 / 0.06
Salticidae / 36 / 7 / 0.64 / 0.60 / -6 / NS
Collembola / 100 / unk. / 47.20 / 42.17 / -11 / NS
Coleoptera5 / 15 / 8 / 0.24 / 0.13 / -46 / NS
Carabidae / 18 / 6 / 0.16 / 0.30 / +88 / NS
Chrysomelidae / 22 / 6 / 1.00 / 0.07 / -93 / 0.002
Curculionidae / 45 / 11 / 0.52 / 1.20 / +131 / 0.07
Scarabaeidae / 22 / 3 / 0.32 / 0.36 / +13 / NS
Tenebrionoidea / 25 / 4 / 0.40 / 0.30 / -25 / NS
Heteroptera5 / 42 / 25 / 1.12 / 0.33 / -71 / 0.007
Aphididae / 20 / 3 / 0.36 / 0.30 / -17 / NS
Cicadoidea / 44 / 18 / 2.44 / 0.40 / -84 / 0.003
Hymenoptera6 / 15 / 4 / 0.20 / 0.27 / +35 / NS
Isopoda / 13 / 2 / 0.24 / 0.93 / +288 / NS
Lepidoptera 5,7 / 15 / 4 / 0.36 / 0.10 / -72 / 0.01
Hesperidae7 / 35 / 1 / 1.08 / 0.17 / -84 / 0.0009
Noctuidae7 / 22 / 5 / 0.60 / 0.30 / -50 / 0.02
Opiliones / 33 / 3 / 1.28 / 0.80 / -38 / 0.0005
Orthoptera / 82 / 7 / 2.08 / 2.93 / +41 / NS
Diplopoda / 20 / 1 / 0.44 / 0.23 / -48 / NS
Chilopoda / 18 / 2 / 0.32 / 0.13 / -59 / NS
Decapoda / 51 / 1 / 1.12 / 0.57 / -49 / NS
Trophic Category
Herbivores / 100 / 79 / 10.1 / 6.2 / -40 / 0.008
Predators / 100 / 60 / 7.6 / 5.0 / -34 / 0.02
Detritivores8 / 100 / 17 / 3.0 / 3.3 / +10 / NS

1 Prevalence – percent of the total stations at which a group was present.

2 Average number of individuals per pitfall trap across all stations in that habitat.

3 The percentage difference between the mean abundance of a group in uninvaded versus invaded habitat.

4 Results of T-tests.

5 All members of group other than those families sufficiently abundant to be listed independently.

6 Wingless “Parasitica”.

7 Larvae only.

8 Excludes Collembola.