Online Resource 1. List of primers used in the analysis

Oligonucleotide combination / Target / Reference
BuRa-16-1 (5’-AGCACTTTTGTCCGGRAA-3’)/
BuRa-16-2 (5’-AATCCCCAACAACTAGTTG-3’) / 16S gene Burkholderia-Ralstonia / Bauernfeind et al. 1999
GB-F (5’-AGTAATACATCGGAACRTGT-3’)/
GB-R (5’-GGSTTGGCRACCCTCTGTT-3’) / 16S gene Burkholderia-Ralstonia / Caballero-Mellado et al. 2007
GB-F (5’-AGTAATACATCGGAACRTGT-3’)/
GBN2-R (5’-GCTCTTGCGTAGCAACTAG-3’) / 16S gene N2-fixing Burkholderia species (except B. vietnamiensis) / Perin et al. 2006a, Caballero-Mellado et al. 2007
fD1 (5’-AGAGTTTGATCCTGGCTCAG-3’)/
rD1 (5’-AAGGAGGTGATCCAGCC-3’) / 16S gene many Bacteria / Weisburg et al. 1991
Btrop-F (5’-TAATACATCGGAACGTGTCCT-3’)/
Btrop-R (5’-GGCCATATTAGGACCAAGGAT-3’) / 16S gene B. tropica / Wong-Villarreal and Caballero-Mellado 2010
Bunam-F (5’-GTGCGCAAGCGGTGATGC-3’)/
Bunam-R (5’-ATGCAGCACCTGTGTTAT-3’) / 16S gene B. unamae / Wong-Villarreal and Caballero-Mellado 2010
Bkuru-F (5’-ATCGGGGCCAATACCCCTGGT-3’)/
Bkuru-R (5’-CCCCGGCTCTCACCAGGGTTC-3’) / 16S gene B. kururiensis / Wong-Villarreal and Caballero-Mellado 2010
Bxeno-F (5’-TCCGGAAAGAAATCCTCTGCC-3’)/
Bxeno-R (5’-TCCACCTCTCAGCAGACTTCC-3’) / 16S gene B. xenovorans / Wong-Villarreal and Caballero-Mellado 2010
Bsilva-F (5’-GGAAAGCGGGGGATCGAAAGA-3’)/
Bsilva-R (5’-AACCAGCTCTCACCGGTCTTC-3’) / 16S gene B. silvatlantica / Wong-Villarreal and Caballero-Mellado 2010
Btuber-F (5’-CAGCACGGACTTCGGTCTGGT-3’)/
Btuber-R (5’-TCACGGATATTAGCCACGAGG-3’) / 16S gene B. tuberum / Wong-Villarreal and Caballero-Mellado 2010
Bphym-F (5’-AAAACTTCGCCGCCAATACCG-3’)/
Bphym-R (5’-GGCAAGGTTCCGTACATGTCA-3’) / 16S gene B. phymatum / Wong-Villarreal and Caballero-Mellado 2010
Bmimo-F (5’-ATCCTCTGGGTTAATACCCCG-3’)/
Bmimo-R (5’-GCTCTCAGCAGGATTCCGTCC-3’) / 16S gene B. mimosarum / Wong-Villarreal and Caballero-Mellado 2010
Bnodo-F (5’-TCCTGGTGGCTAATATCCGTC-3’)/
Bnodo-R (5’-TTCCACCTCTCAGCGGAATTC-3’) / 16S gene B. nodosa / Wong-Villarreal and Caballero-Mellado 2010
Burkf (5’-GGCGAAAGCCGGATTAATACC-3’)/
CeMuVi-16-2 (5’-CCGRCTGTATTAGAGCCA-3’) / 16S gene B. cepacia complex / Bauernfeind et al. 1999, Ramette et al. 2005
BCR1 (5’-TGACCGCCGAGAAGAGCAA-3’)/
BCR2 (5’-CTCTTCTTCGTCCATCGCCTC-3’) / recA gene B. cepacia complex / Mahenthiralingam et al. 2000
IGK (5’-TACGGYAARGGBGGYATCGG-3’)/
NDR-1 (5’-TTGGAGCCGGCRTANGCRCA-3’) / nifH gene many N2-fixing Bacteria / Poly et al. 2001, Valdes et al. 2005
CBL1 (5’-CCAAAGGACTAACCCA-3’)/
CBL2 (5’-ACGCGATGTCCATCACA-3’) / cblA gene B. cepacia complex / Sajjan et al. 1995
BCESM1 (5’- CCACGGACGTGACTAACA-3’)/
BCSEM2 (5’- CGTCCATCCGAACACGAT-3’) / esmR gene B. cepacia complex / Mahenthiralingam et al. 1997
5’-ACC (5’ACGCCGATCCARCCGCTM-3’)/
3’-ACC (5’TCCAGCGTGCCSTCGTTC3-3’) / acdS gene / Caballero-Mellado et al. 2007