One Shot INV F Competent E. Coliinvitrogen

2 Materials

2 Materials

Bacteria

One Shot INVF competent E. coliInvitrogen

cDNA and plasmides

Human -actin cDNAClontech

Serglycin cDNAKolset, University of Oslo

pcDNA3.1(-)/Myc-His A,B,CInvitrogen

pEGFP-N1Clontech

PCR primersOligon AS, Forskningsparken

Cell lines

HAECATCC

HUVECATCC

MDCK IIATCC

U937-1Dr. K. Nilsson, Uppsala, Sweeden

Cell growth equipment/medium

AmpicillinServa

Culture flasksCostar

DMEM (Dulbecco’s Modified Eagle’s Medium)BioWhittaker

Endothelial Cell Growth Supplement (ECGS)GibcoBRL

Endothelial-SFM MediumGibcoBRL

Fetal calf serum (FCS)BioWhittaker

Gelatin (2 % solution)Sigma

Geneticin (G-418) GibcoBRL,LifeTechnologies

L-GlutamineBioWhittaker

LipofectaminGibcoBRL

LipofectinGibcoBRL

Low Serum Growth Supplement (LSGS)ATCC

Medium 200 (M200)ATCC

OPTI-MEM (serum free medium)GibcoBRL

Penicillin-StreptomycinBioWhittaker

RPMI 1640 (sulphate free)GibcoBRL

RPMI 1640BioWhittaker

Trypsin Neutraliser (R-002)ATCC

TrypsinDifco

Trypsin/EDTA (R-001)ATCC

Chemicals

AgaroseMedProbe

AprotininSigma

ATPPromega

Ba(NO2)2Fluka

Bacto-agarDifco

Bacto-tryptoneDifco

Bacto-yeast extractMerck

Blue DextranSigma

Bromophenol blueBioRad

BSA (bovine Serum albumine), No: A-7906Sigma

CaCl2  2 H2OMerck

CaSO4  5 H2OMerck

CH3COOK (Potassium acetate)Merck

CHAPSSigma

ChloroformMerck

Co(NO3)2  6 H2OMerck

dATPPromega

dCTPPromega

DEPC (dietyl pyrocarbonate)Sigma

dGTPPromega

DMSO (dimethyl sulphoxide)Sigma

dTTPPromega

Dynabeads Oligo (dT)25Dynal

EDTA (Triplex III)Merck

EtBr (ethidium bromide)Sigma

EthanolArcus

FormaldehydePROLABO

FormamideFluka

Glacial acetic acidMerck

GlucoseSigma

GlycerolNorsk Medesinaldepot

GlycineSigma

GuanidineSigma

H2SO4Merck

HClMerck

HEPESSigma

ImidazoleMerck

Insta-gel II Plus, scintillation fluidPackard

KClMerck

KH2PO4Merck

Low melting agaroseInternational Biotehnologies

MethanolMerck

-MercaptoethanolSigma

MgCl2Merck

MOPS (3-N-Morfolino-propanesulfonic acid)Sigma

Na2HPO4Merck

NaClMerck

NaOHKeboLab

NiCl2Merck

PBS/DPBS (phosphate buffered saline)BioWhittaker

PhenolMerck

RNase AWAYMolecular Bio Products

SDS (sodium dodecyl sulphate)Sigma

Sodium acetateMerck

Tris baseSigma

TrisHClSigma

Triton-X100KeboLab

Tween 20KeboLab

X-GalPromega

Enzymes / growth factors

Restriction enzymes from Promega

Enzyme Promega buffer

EcoRIBuffer C

BamHIBuffer C

XbaIBuffer C

XholBuffer C

XhoIBuffer D

Bacteriophage T4 DNA ligasePromega

Chondroitinase ABC lyaseSeikagaku Corporation

Klenow fragment (from E. coli DNA polymerase)Promega

RNAseAQiagen

Taq-polymerasePerkin Elmer

TGF-R&D Systems

Equipment

4-20 % Tris-Glycine gelNovex

DEAE Sephacel (anion exchanger matrix)Pharmacia Biotech

Dynabeads Oligo (dT)25Dynal

Dynal MPCDynal

Hi-Trap chelating column (1 ml)Pharmacia biotech

Hybond-N (nylon membrane)Amersham

Sephadex G-50 FinePharmacia biotech

Superose 6 HR 10/30 columnPharmacia biotech

Transwell culture plates, 12 and 24 mm diameterCostar

Transwell polycarbonate membraneCostar

(Cat. No. 3412, 0,4 mm pore size)

Instruments

1414 Win Spectral Scintillation counterWallac

DNA Speedvac DNA 100Savant, Houm

FluorImagerMolecular Dynamics

FPLC systemPharmacia biotech

Gel dryer Model 583BioRad

Gene Quant RNA/DNA CalculatorPharmacia Biotech

IEC Centra-M CentrifugeHeigar

Megafuge 1.0RHeraeus

PhosphorImagerMolecular Dynamics

VirSonic 50Houm

XCELL (electrophoresis chamber)Novex

Kits

Probe Quant G50 Micro ColumnsPharmacia Biotech

Qiagen Plasmid Maxi Preparation KitQiagen

Size Sep Spun ColumnsPharmacia Biotech

TA Cloning KitInvitrogen

Wizard DNA Clean Up SystemPromega

WizardTM PCR Preps DNA purification SystemPromega

Molecular weight standards

174/RF DNA / HaeIII fragmentsPromega

-DNA / HindIII fragmentsPromega

Rainbow 14C metylated standard (15-200 kDa)Amersham

Radiolabelled chemicals

[35S] Sodium sulphate ( 35S(sulphate))Amersham

[32P]-dCTP (32P(phosphate))Amersham

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2 Materials

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