2 Materials
2 Materials
Bacteria
One Shot INVF competent E. coliInvitrogen
cDNA and plasmides
Human -actin cDNAClontech
Serglycin cDNAKolset, University of Oslo
pcDNA3.1(-)/Myc-His A,B,CInvitrogen
pEGFP-N1Clontech
PCR primersOligon AS, Forskningsparken
Cell lines
HAECATCC
HUVECATCC
MDCK IIATCC
U937-1Dr. K. Nilsson, Uppsala, Sweeden
Cell growth equipment/medium
AmpicillinServa
Culture flasksCostar
DMEM (Dulbecco’s Modified Eagle’s Medium)BioWhittaker
Endothelial Cell Growth Supplement (ECGS)GibcoBRL
Endothelial-SFM MediumGibcoBRL
Fetal calf serum (FCS)BioWhittaker
Gelatin (2 % solution)Sigma
Geneticin (G-418) GibcoBRL,LifeTechnologies
L-GlutamineBioWhittaker
LipofectaminGibcoBRL
LipofectinGibcoBRL
Low Serum Growth Supplement (LSGS)ATCC
Medium 200 (M200)ATCC
OPTI-MEM (serum free medium)GibcoBRL
Penicillin-StreptomycinBioWhittaker
RPMI 1640 (sulphate free)GibcoBRL
RPMI 1640BioWhittaker
Trypsin Neutraliser (R-002)ATCC
TrypsinDifco
Trypsin/EDTA (R-001)ATCC
Chemicals
AgaroseMedProbe
AprotininSigma
ATPPromega
Ba(NO2)2Fluka
Bacto-agarDifco
Bacto-tryptoneDifco
Bacto-yeast extractMerck
Blue DextranSigma
Bromophenol blueBioRad
BSA (bovine Serum albumine), No: A-7906Sigma
CaCl2 2 H2OMerck
CaSO4 5 H2OMerck
CH3COOK (Potassium acetate)Merck
CHAPSSigma
ChloroformMerck
Co(NO3)2 6 H2OMerck
dATPPromega
dCTPPromega
DEPC (dietyl pyrocarbonate)Sigma
dGTPPromega
DMSO (dimethyl sulphoxide)Sigma
dTTPPromega
Dynabeads Oligo (dT)25Dynal
EDTA (Triplex III)Merck
EtBr (ethidium bromide)Sigma
EthanolArcus
FormaldehydePROLABO
FormamideFluka
Glacial acetic acidMerck
GlucoseSigma
GlycerolNorsk Medesinaldepot
GlycineSigma
GuanidineSigma
H2SO4Merck
HClMerck
HEPESSigma
ImidazoleMerck
Insta-gel II Plus, scintillation fluidPackard
KClMerck
KH2PO4Merck
Low melting agaroseInternational Biotehnologies
MethanolMerck
-MercaptoethanolSigma
MgCl2Merck
MOPS (3-N-Morfolino-propanesulfonic acid)Sigma
Na2HPO4Merck
NaClMerck
NaOHKeboLab
NiCl2Merck
PBS/DPBS (phosphate buffered saline)BioWhittaker
PhenolMerck
RNase AWAYMolecular Bio Products
SDS (sodium dodecyl sulphate)Sigma
Sodium acetateMerck
Tris baseSigma
TrisHClSigma
Triton-X100KeboLab
Tween 20KeboLab
X-GalPromega
Enzymes / growth factors
Restriction enzymes from Promega
Enzyme Promega buffer
EcoRIBuffer C
BamHIBuffer C
XbaIBuffer C
XholBuffer C
XhoIBuffer D
Bacteriophage T4 DNA ligasePromega
Chondroitinase ABC lyaseSeikagaku Corporation
Klenow fragment (from E. coli DNA polymerase)Promega
RNAseAQiagen
Taq-polymerasePerkin Elmer
TGF-R&D Systems
Equipment
4-20 % Tris-Glycine gelNovex
DEAE Sephacel (anion exchanger matrix)Pharmacia Biotech
Dynabeads Oligo (dT)25Dynal
Dynal MPCDynal
Hi-Trap chelating column (1 ml)Pharmacia biotech
Hybond-N (nylon membrane)Amersham
Sephadex G-50 FinePharmacia biotech
Superose 6 HR 10/30 columnPharmacia biotech
Transwell culture plates, 12 and 24 mm diameterCostar
Transwell polycarbonate membraneCostar
(Cat. No. 3412, 0,4 mm pore size)
Instruments
1414 Win Spectral Scintillation counterWallac
DNA Speedvac DNA 100Savant, Houm
FluorImagerMolecular Dynamics
FPLC systemPharmacia biotech
Gel dryer Model 583BioRad
Gene Quant RNA/DNA CalculatorPharmacia Biotech
IEC Centra-M CentrifugeHeigar
Megafuge 1.0RHeraeus
PhosphorImagerMolecular Dynamics
VirSonic 50Houm
XCELL (electrophoresis chamber)Novex
Kits
Probe Quant G50 Micro ColumnsPharmacia Biotech
Qiagen Plasmid Maxi Preparation KitQiagen
Size Sep Spun ColumnsPharmacia Biotech
TA Cloning KitInvitrogen
Wizard DNA Clean Up SystemPromega
WizardTM PCR Preps DNA purification SystemPromega
Molecular weight standards
174/RF DNA / HaeIII fragmentsPromega
-DNA / HindIII fragmentsPromega
Rainbow 14C metylated standard (15-200 kDa)Amersham
Radiolabelled chemicals
[35S] Sodium sulphate ( 35S(sulphate))Amersham
[32P]-dCTP (32P(phosphate))Amersham
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2 Materials
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