Oligonucleotides Used in This Work

Table S1

Oligonucleotides used in this work

Name /

Sequence

/ Use
A1F / 5’agttatgagttgggcaaacactt 3’ / Isolation of Ot-eft-2
A1R / 5’GATCAAATAACCATCCGGAAACAA 3’
E1F / 5’gattctttcgtcgctctaac 3’ / Isolation of Ot-eft-3
E1R / 5’TTTCATCAAAATTAAGGCACTCA 3’
E2F / 5’GTTGCTTCTAAGCTCAACTACCA 3’ / Isolation of Ot-eft-4
E2R / 5’TTAATCAAACTAGCCAGAGAATCA 3’
PXT1 / 5’CCTTTCCCATGGTGGCTC 3’ / Primer extension of Ot-eft-1
PXT2 / 5’CGGTGGTGGTGGACTTTC 3’

Table S2

Codon usage of O. tipulae and C. elegans eEF1A genes

O. tipulae / C. elegans /

TOTAL

Aa / Codon / Ot-eft-1 / Ot-eft-2 / Ot-eft-3 / Ot-eft-4 / Ce-eft-3 / Ce-eft-4 / Ot-eft / Ce-eft
n / % / n / % / n / % / n / % / n / % / n / % / n / % / n / %
Ala(A) / GCA
GCC
GCG
GCU
Total / 1
20
0
11
32 / 1.1
62.5
0.0
34.3 / 0
21
0
12
33 / 0.0
63.6
0.0
36.4 / 2
14
1
14
31 / 6.4
45.2
3.2
45.2 / 0
18
0
13
31 / 0.0
58.1
0.0
41.9 / 1
16
0
15
32 / 3.1
50.0
0.0
46.9 / 0
17
0
15
32 / 0.0
53.1
0.0
46.9 / 3
73
1
50
127 / 2.4
57.5
0.8
39.4 / 1
33
0
30
64 / 1.6
51.6
0.0
46.9
Arg(R) / AGA
AGG
CGA
CGC
CGG
CGU
Total / 5
0
0
2
0
11
18 / 27.8
0.0
0.0
11.1
0.0
61.1 / 5
0
0
2
0
11
18 / 27.8
0.0
0.0
11.1
0.0
61.1 / 3
1
0
1
1
12
18 / 16.6
5.6
0.0
5.6
5.6
66.6 / 3
0
0
2
0
13
18 / 16.6
0.0
0.0
11.1
0.0
72.2 / 2
0
1
4
0
12
19 / 10.5
0.0
5.3
21.0
0.0
63.1 / 0
0
1
3
0
15
19 / 0.0
0.0
5.3
15.8
0.0
78.9 / 16
1
0
7
1
47
72 / 22.2
1.4
0.0
9.7
1.4
65.3 / 2
0
2
7
0
27
38 / 5.3
0.0
5.3
18.4
0.0
71.0
Asn(N) / AAC
AAU
Total / 11
3
14 / 78.6
21.4 / 12
3
15 / 80.0
20.0 / 14
2
16 / 87.5
12.5 / 12
3
15 / 80.0
20.0 / 16
1
17 / 94.1
5.9 / 15
2
17 / 88.2
11.8 / 49
11
60 / 81.6
18.3 / 31
3
34 / 91.2
8.8
Asp(D) / GAC
GAU
Total / 16
8
24 / 66.7
33.3 / 19
5
24 / 79.2
20.8 / 21
4
25 / 84.0
16.0 / 17
7
24 / 70.1
29.2 / 14
9
23 / 60.9
39.1 / 13
10
23 / 56.5
43.5 / 73
24
97 / 75.2
24.7 / 27
19
46 / 58.7
41.3
Cys(C) / UGC
UGU
Total / 9
0
9 / 100
0.0 / 9
0
9 / 100
0.0 / 8
1
9 / 88.9
11.1 / 9
0
9 / 100
0.0 / 7
1
8 / 87.5
12.5 / 8
0
8 / 100
0.0 / 35
1
36 / 97.2
2.7 / 15
1
16 / 93.7
6.2
Gln(Q) / CAA
CAG
Total / 0
12
12 / 0.0
100 / 1
12
13 / 7.7
92.3 / 4
7
11 / 36.4
63.6 / 2
11
13 / 15.4
84.6 / 7
6
13 / 53.8
46.1 / 7
6
13 / 53.8
46.1 / 7
42
49 / 14.3
85.7 / 14
12
26 / 53.8
46.2
Glu(E) / GAA
GAG
Total / 9
22
31 / 29.0
10.9 / 9
22
31 / 29.0
10.9 / 4
25
29 / 13.8
86.2 / 9
23
32 / 28.1
71.9 / 7
14
21 / 33.3
66.6 / 7
24
31 / 22.6
77.4 / 31
92
123 / 25.2
74.8 / 14
48
62 / 22.6
77.4
Gly(G) / GGA
GGC
GGG
GGU
Total / 37
2
0
4
43 / 86.0
4.6
0.0
9.3 / 40
0
0
3
43 / 93.0
0.0
0.0
7.0 / 24
13
1
4
42 / 57.1
30.9
2.4
9.5 / 35
3
0
4
42 / 83.3
7.14
0.0
9.5 / 38
0
0
3
41 / 92.7
0.0
0.0
7.3 / 39
0
0
2
41 / 95.1
0.0
0.0
4.9 / 136
18
1
15
170 / 80.0
10.6
0.6
8.8 / 77
0
0
5
82 / 93.9
0.0
0.0
6.1
His(H) / CAC
CAU
Total / 8
2
10 / 80.0
20.0 / 8
2
10 / 80.0
20.0 / 9
1
10 / 90.0
10.0 / 7
3
10 / 70.0
30.0 / 7
4
11 / 63.6
36.4 / 6
5
11 / 54.5
45.4 / 32
8
40 / 80.0
20.0 / 13
9
22 / 59.1
40.9
Ile(I) / AUA
AUC
AUU
Total / 0
28
5
33 / 0.0
84.8
15.1 / 0
27
6
33 / 0.0
81.8
18.2 / 0
23
8
31 / 0.0
74.2
25.8 / 0
28
5
33 / 0.0
84.8
15.2 / 0
31
3
34 / 0.0
91.2
8.8 / 0
26
8
34 / 0.0
76.5
23.5 / 0
106
24
130 / 0.0
81.5
18.5 / 0
57
11
68 / 0.0
83.8
16.2
Leu / CUA
CUC
CUG
CUU
UUA
UUG
Total / 0
15
4
2
0
2
23 / 0.0
65.2
17.4
8.7
0.0
8.7 / 0
15
2
4
0
2
23 / 0.0
65.2
8.7
17.4
0.0
8.7 / 0
12
3
2
0
5
22 / 0.0
54.5
13.6
9.1
0.0
22.7 / 0
12
3
6
0
2
23 / 0.0
52.2
13.0
26.1
0.0
8.7 / 0
13
1
7
0
2
23 / 0.0
56.5
4.3
30.4
0.0
8.7 / 0
11
1
9
0
2
23 / 0.0
47.8
4.3
39.1
0.0
8.7 / 0
54
12
14
0
11
91 / 0.0
59.3
13.2
15.4
0.0
12.1 / 0
24
2
16
0
4
46 / 0.0
52.2
4.3
34.8
0.0
8.7
Lys(K) / AAA
AAG
Total / 4
44
48 / 8.3
91.6 / 2
44
46 / 4.3
95.7 / 10
36
46 / 21.7
78.3 / 4
43
47 / 8.5
91.5 / 1
45
46 / 2.2
97.8 / 1
45
46 / 2.2
97.8 / 20
167
187 / 10.7
89.3 / 2
90
92 / 2.2
97.8
Met(M) / AUG
Total / 1111 / 100 / 11
11 / 100 / 11
11 / 100 / 11
11 / 100 / 10
10 / 100 / 10
10 / 100 / 44
44 / 100 / 20
20 / 100
Phe(F) / UUC
UUU
Total / 18
0
18 / 100
0.0 / 18
0
18 / 100
0.0 / 18
0
18 / 100
0.0 / 18
0
18 / 100
0.0 / 19
0
19 / 100
0.0 / 18
1
19 / 94.7
5.3 / 72
0
72 / 100
0.0 / 37
1
38 / 97.4
2.6
Pro(P) / CCA
CCC
CCG
CCU
Total / 4
12
0
8
24 / 16.7
50.0
0.0
33.3 / 3
12
0
8
23 / 13.0
52.2
0.0
34.8 / 3
11
5
5
24 / 12.5
45.8
20.8
20.8 / 3
12
1
8
24 / 12.5
50.0
4.2
33.3 / 25
0
0
0
25 / 100
0.0
0.0
0.0 / 25
0
0
0
25 / 100
0.0
0.0
0.0 / 13
47
6
29
95 / 13.7
49.5
6.3
30.5 / 50
0
0
0
50 / 100
0.0
0.0
0.0
Ser(S) / AGC
AGU
UCA
UCC
UCG
UCU
Total / 2
1
0
16
3
2
24 / 8.3
4.2
0.0
66.7
12.5
8.3 / 2
0
0
17
3
3
25 / 8.0
0.0
0.0
68.0
12.0
12.0 / 3
2
1
7
9
8
30 / 10.0
6.7
3.3
23.3
30.0
26.7 / 2
2
0
14
3
4
25 / 8.0
8.0
0.0
56.0
12.0
16.0 / 0
0
0
19
0
8
27 / 0.0
0.0
0.0
70.4
0.0
29.6 / 0
0
1
22
1
3
27 / 0.0
0.0
3.7
81.5
3.7
11.1 / 9
5
1
54
18
17
104 / 8.6
4.8
1.0
51.9
17.3
16.3 / 0
0
1
41
1
11
54 / 0.0
0.0
1.8
75.9
1.8
20.4
Stop / UAA
UAG
UGA
Total / 1
0
0
1 / 100
0.0
0.0 / 1
0
0
1 / 100
0.0
0.0 / 1
0
0
1 / 100
0.0
0.0 / 0
0
0
0 / 1
0
0
1 / 100
0.0
0.0 / 1
0
0
1 / 100
0.0
0.0 / 4
0
0
4 / 100
0.0
0.0 / 2
0
0
2 / 100
0.0
0.0
Thr(T) / ACA
ACC
ACG
ACU
Total / 1
20
0
11
32 / 3.1
62.5
0.0
34.4 / 1
17
1
13
32 / 3.1
53.1
3.1
40.6 / 1
19
5
8
33 / 3.1
57.6
15.1
24.2 / 0
12
0
9
21 / 0.0
57.1
0.0
42.8 / 2
19
0
9
30 / 6.7
63.3
0.0
30 / 0
24
0
6
30 / 0.0
80.0
0.0
20 / 3
76
7
43
129 / 2.3
58.9
5.4
33.3 / 2
43
0
15
60 / 3.3
71.7
0.0
25
Trp(W) / UGG
Total / 4
4 / 100 / 4
4 / 100 / 4
4 / 100 / 4
4 / 100 / 4
4 / 100 / 4
4 / 100 / 16
16 / 100 / 8
8 / 100
Tyr(Y) / UAC
UAU
Total / 8
0
8 / 100
0.0 / 8
0
8 / 100
0.0 / 8
0
8 / 100
0.0 / 6
0
6 / 100
0.0 / 8
0
8 / 100
0.0 / 8
0
8 / 100
0.0 / 32
0
32 / 100
0.0 / 16
0
16 / 100
0.0
Val(V) / GUA
GUC
GUG
GUU
Total / 1
31
3
6
41 / 2.4
75.6
7.3
14.6 / 0
32
2
6
40 / 0.0
80.0
5.0
15.0 / 3
26
8
5
42 / 7.1
61.9
19.0
11.9 / 1
18
1
1
21 / 4.8
85.7
4.8
4.8 / 0
24
1
17
42 / 0.0
57.1
2.4
40.5 / 0
28
2
12
42 / 0.0
66.7
4.8
28.6 / 5
119
15
24
163 / 3.1
73.0
9.2
14.7 / 0
52
3
29
84 / 0.0
61.9
3.6
34.5

Table S3

Sequences used for the alignement

gene name / WormBase / nr acc. / contig / Coding region used for alignment

Cbr-eft-3

/ CBG24566 / CAAC01000333 / cb25.NA_191 / complement(join(8308..8451,8092..8260,7225..8045,6921..7178))
Cbr-eft-4 / CBG16828 / CAAC01000078 / cb25.fpc4044 / join(1617543..1617855,1617963..1618783,1618835..1619092)
Cel-eft-3 / F31E3.5 / U40935 / complement(join(7662..7919,7969..8789,8838..9006,9060..9203))
Cel-eft-4 / R03G5.1a / U51994 / join(26515..26827,26978..27798,27848..28105)
Oti-eft-1 / AY928342 / join(732..872,916..1458,1500..1703,1747..2151,2194..2280)
Oti-eft-2 / AY928338 / join(36..176,280..822,864..1100,1295..1402,1444..1707,1750..1836)
Oti-eft-4 / AY928337 / join(27..57,99..208,286..828,870..1106,1300..1407,1448..1798)
Oti-eft-3 / AY928340 / join(41..165,234..292,621..739,779..933,1006..1234,1274..1882,1925..2011)
Ppa-eft-1 / BK006064 / 73..1473
Aca-eft-1 / BK006068 / 51..1448
Ace-eft-1 / BK006070 / 83..1477
Aca-eft-2 / BK006069 / 3..1385
Ace-eft-2 / BK006060 / 86..1483
Gpa-eft-1 / BK006062 / 42..1439
Hgl-eft-1 / BK006063 / 12..1409
Asu-eft-1 / BK006061 / 67..1461
Xin-eft-1 / BK006067 / 61..1461
Xin-eft-2 / BK006059 / 30..1430
Sst-eft-1 / BK006065 / 35..1423
Sst-eft-2 / BK006066 / 28..1416
Ovo-eft-1 / M64333 / 52..1446

Table S4

Pairwise distances derived from RSCU values

CBR3 / CBR4 / CEL3 / CEL4 / OTI1 / OTI2 / OTI4 / OTI3 / PPAC / ANC1 / ACE1 / ANC2 / ACE2 / GPAL / HGLY / ASUU / XIN1 / XIN2 / SST1 / SST2 / OVOL

Cbr-eft-3

/ -
Cbr-eft-4 / 0,142 / -
Cel-eft-3 / 0,181 / 0,161 / -
Cel-eft-4 / 0,178 / 0,159 / 0,174 / -
Oti-eft-1 / 0,382 / 0,334 / 0,396 / 0,434 / -
Oti-eft-2 / 0,365 / 0,314 / 0,381 / 0,410 / 0,086 / -
Oti-eft-4 / 0,337 / 0,334 / 0,359 / 0,372 / 0,131 / 0,144 / -
Oti-eft-3 / 0,483 / 0,502 / 0,514 / 0,567 / 0,372 / 0,388 / 0,327 / -
Ppa-eft-1 / 0,414 / 0,352 / 0,431 / 0,470 / 0,278 / 0,266 / 0,277 / 0,406 / -
Aca-eft-1 / 0,477 / 0,494 / 0,461 / 0,457 / 0,497 / 0,519 / 0,421 / 0,416 / 0,464 / -
Ace-eft-1 / 0,481 / 0,497 / 0,449 / 0,461 / 0,478 / 0,497 / 0,401 / 0,402 / 0,461 / 0,029 / -
Aca-eft-2 / 0,497 / 0,548 / 0,453 / 0,525 / 0,415 / 0,429 / 0,352 / 0,433 / 0,501 / 0,254 / 0,242 / -
Ace-eft-2 / 0,531 / 0,591 / 0,487 / 0,538 / 0,474 / 0,487 / 0,406 / 0,454 / 0,535 / 0,250 / 0,238 / 0,126 / -
Gpa-eft-1 / 0,794 / 0,833 / 0,807 / 0,839 / 0,752 / 0,744 / 0,689 / 0,526 / 0,759 / 0,563 / 0,559 / 0,574 / 0,558 / -
Hgly-eft-1 / 0,716 / 0,755 / 0,689 / 0,756 / 0,757 / 0,741 / 0,684 / 0,536 / 0,768 / 0,526 / 0,522 / 0,531 / 0,517 / 0,274 / -
Asu-eft-1 / 0,786 / 0,815 / 0,793 / 0,823 / 0,761 / 0,776 / 0,711 / 0,540 / 0,787 / 0,532 / 0,541 / 0,475 / 0,493 / 0,353 / 0,368 / -
Xin-eft-1 / 0,820 / 0,810 / 0,803 / 0,816 / 0,779 / 0,817 / 0,748 / 0,629 / 0,843 / 0,607 / 0,579 / 0,640 / 0,636 / 0,547 / 0,503 / 0,570 / -
Xin-eft-2 / 0,826 / 0,816 / 0,809 / 0,822 / 0,779 / 0,817 / 0,748 / 0,620 / 0,843 / 0,609 / 0,582 / 0,643 / 0,639 / 0,548 / 0,497 / 0,561 / 0,017 / -
Sst-eft-1 / 0,685 / 0,718 / 0,606 / 0,668 / 0,834 / 0,825 / 0,785 / 0,806 / 0,928 / 0,632 / 0,634 / 0,661 / 0,625 / 0,845 / 0,662 / 0,871 / 0,813 / 0,815 / -
Sst-eft-2 / 0,821 / 0,855 / 0,748 / 0,817 / 0,955 / 0,945 / 0,922 / 0,912 / 1,053 / 0,749 / 0,751 / 0,765 / 0,732 / 0,882 / 0,702 / 0,915 / 0,866 / 0,868 / 0,185 / -
Ovo-eft-1 / 1,062 / 1,114 / 1,032 / 1,091 / 1,102 / 1,098 / 1,035 / 0,933 / 1,176 / 0,815 / 0,811 / 0,750 / 0,749 / 0,759 / 0,617 / 0,670 / 0,822 / 0,806 / 0,755 / 0,683 / -

Data obtained from the coding region of each of the genes (see Table S3) using the multivariate analysis module of the software CGUA and calculating the distance between the mean RSCU of a pair of sequences.

Table S5

Percent Identity Matrixa for nematodeb eEF1A sequences

Cbr / Cel / Oti / Ppa / Aca / Ace / Sst / Asu / Ovo / Gpa / Hgl / Xin
eft-4 / eft-3 / eft-3 / eft-4 / eft-1 / eft-2 / etf-3 / eft-4 / eft-1 / eft-1 / eft-2 / eft-1 / eft-2 / eft-1 / sst-2 / eft-1 / eft-1l / eft-1 / eft-1 / eft-1 / eft-2
Cbr / eft-4 / ----- / 100.0 / 98.7 / 98.7 / 90.9 / 91.1 / 89.8 / 91.5 / 93.5 / 95.4 / 94.4 / 95.4 / 95.0 / 90.7 / 88.5 / 90.3 / 87.7 / 88.4 / 88.6 / 84.7 / 84.7
eft-3 / 95.4 / ----- / 98.7 / 98.7 / 90.9 / 91.1 / 89.8 / 91.5 / 93.5 / 95.4 / 94.4 / 95.4 / 95.0 / 90.7 / 88.5 / 90.3 / 87.7 / 88.4 / 88.6 / 84.7 / 84.7
Cel / eft-3 / 93.8 / 94..5 / ----- / 100.0 / 90.7 / 90.9 / 90.3 / 91.3 / 92.9 / 94.6 / 93.5 / 94.6 / 94.4 / 89.8 / 87.7 / 89.8 / 87.9 / 88.8 / 88.6 / 84.5 / 84.5
eft-4 / 94.6 / 93.5 / 93.9 / ----- / 90.7 / 90.9 / 90.3 / 91.3 / 92.9 / 94.6 / 93.5 / 94.6 / 94.4 / 89.8 / 87.7 / 89.8 / 87.9 / 88.8 / 88.6 / 84.5 / 84.5
eft-1 / 86.4 / 85.2 / 85.1 / 85.4 / ----- / 99.1 / 95.6 / 98.6 / 89.9 / 90.7 / 90.1 / 90.7 / 90.3 / 89.8 / 87.9 / 88.1 / 86.6 / 88.4 / 87.7 / 83.9 / 83.9
Oti / eft-2 / 86.4 / 85.5 / 85.0 / 85.3 / 96.3 / ----- / 96.0 / 99.1 / 90.1 / 90.9 / 90.3 / 90.9 / 90.5 / 90.0 / 88.1 / 88.3 / 86.8 / 88.6 / 87.9 / 84.1 / 84.1
eft-3 / 81.7 / 81.0 / 80.5 / 80.4 / 85.9 / 86.2 / ----- / 95.6 / 89.0 / 89.8 / 89.0 / 89.8 / 89.6 / 87.7 / 85.7 / 86.6 / 85.4 / 87.1 / 86.0 / 84.1 / 84.1
eft-4 / 86.1 / 84.9 / 84.8 / 85.1 / 95.5 / 95.3 / 86.1 / ----- / 90.1 / 91.1 / 90.3 / 91.1 / 90.5 / 90.2 / 88.3 / 88.6 / 87.0 / 88.8 / 88.1 / 84.5 / 84.5
Ppa / eft-1 / 87.0 / 87.3 / 86.2 / 85.1 / 84.7 / 84.7 / 80.1 / 83.2 / ----- / 93.7 / 93.1 / 93.7 / 93.9 / 89.9 / 87.7 / 90.1 / 87.3 / 89.4 / 89.4 / 84.9 / 84.9
Aca / eft-1 / 86.6 / 86.6 / 86.3 / 85.4 / 82.5 / 81.8 / 79.7 / 81.7 / 86.3 / ----- / 97.8 / 100.0 / 98.4 / 92.6 / 90.5 / 92.9 / 89.8 / 89.6 / 89.9 / 86.2 / 86.2
eft-2 / 84.5 / 84.5 / 84.1 / 83.8 / 81.6 / 81.4 / 79.5 / 80.6 / 86.5 / 88.1 / ----- / 97.8 / 99.1 / 92.0 / 89.8 / 93.3 / 90.1 / 89.0 / 89.4 / 85.6 / 85.6
Ace / eft-1 / 86.6 / 86.5 / 86.3 / 85.4 / 82.6 / 81.9 / 79.8 / 81.7 / 86.2 / 99.5 / 88.1 / ----- / 98.4 / 92.6 / 90.5 / 92.9 / 89.8 / 89.6 / 89.9 / 86.2 / 86.2
eft-2 / 84.4 / 84.1 / 84.1 / 84.0 / 81.7 / 81.6 / 79.4 / 80.8 / 85.2 / 88.5 / 95.8 / 88.5 / ----- / 92.2 / 90.1 / 93.5 / 90.5 / 89.2 / 89.6 / 85.8 / 85.8
Sst / eft-1 / 79.8 / 79.1 / 79.3 / 79.8 / 77.8 / 77.9 / 75.4 / 77.2 / 76.3 / 78.8 / 80.1 / 79.1 / 80.3 / ----- / 97.8 / 91.8 / 88.5 / 90.1 / 89.8 / 85.1 / 85.1
eft-2 / 77.2 / 77.0 / 77.5 / 76.7 / 75.5 / 74.9 / 72.7 / 74.7 / 74.3 / 76.8 / 78.0 / 76.9 / 78.1 / 94.3 / ----- / 89.6 / 86.4 / 88.1 / 87.9 / 83.6 / 83.6
Asu / eft-1 / 76.8 / 77.1 / 75.6 / 77.0 / 75.3 / 75.4 / 74.5 / 75.3 / 77.9 / 79.4 / 79.8 / 79.4 / 80.2 / 75.5 / 73.4 / ----- / 93.1 / 90.9 / 91.1 / 87.1 / 87.1
Ovo / eft-1 / 71.1 / 71.3 / 71.2 / 70.9 / 69.6 / 70.0 / 69.0 / 69.8 / 70.5 / 74.1 / 74.7 / 74.1 / 74.8 / 75.2 / 75.0 / 75.3 / ----- / 88.1 / 88.8 / 86.4 / 86.4
Gpa / eft-1 / 76.5 / 76.8 / 76.5 / 75.8 / 76.5 / 76.8 / 74.5 / 75.7 / 78.7 / 77.8 / 77.5 / 78.0 / 76.9 / 74.1 / 73.3 / 78.8 / 74.7 / ----- / 95.6 / 86.6 / 86.6
Hgl / eft-1 / 76.9 / 77.2 / 76.9 / 76.5 / 75.7 / 76.1 / 73.3 / 75.6 / 78.0 / 78.0 / 77.1 / 78.2 / 76.8 / 75.6 / 74.4 / 78.5 / 74.8 / 85.7 / ----- / 86.6 / 86.6
Xin / eft-1 / 75.0 / 74.7 / 74.7 / 74.2 / 73.4 / 73.2 / 72.0 / 73.6 / 75.3 / 76.5 / 75.7 / 76.4 / 75.0 / 75.0 / 74.4 / 75.5 / 73.0 / 76.4 / 75.8 / ----- / 100.0
eft-2 / 75.0 / 74.8 / 74.8 / 74.3 / 73.5 / 73.3 / 72.0 / 73.8 / 75.3 / 76.5 / 75.8 / 76.4 / 74.9 / 75.0 / 74.4 / 75.5 / 73.1 / 76.4 / 75.9 / 99..7 / -----

aupper half= protein sequences (bold); lower half= nucleotide sequences.

bCbr=Caenorhabditis briggsae; Cel=Caenorhabditis elegans; Oti=Oscheius tipulae; Ppa=Pristionchus pacificus; Aca=Ancylostoma caninum; Ace=Ancylostoma ceylanicum; Sst=Strongyloides stercoralis; Asu=Ascaris suum; Ovo=Onchocerca volvulus; Gpa=Globodera pallida; Hgl=Heterodera glycines; Xin=Xiphinema index.

Figure S1 - Tree constructed using data shown in Table S4, formated for PAUP 4.0. This is an unrooted distance tree. The scale bar shows distance in RSCU differences.