OIE RL for «Taura Syndrome Disease of crustaceans » – «Donald V. Lightner » – «United States of America»

OIE Reference Laboratory Reports
Activities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Taura Syndrome Disease of crustaceans
Address of laboratory / Aquaculture Pathology Laboratory, Veterinary Science and Microbiology, University of Arizona, Tucson, AZ85721USA
Tel.: / 001 520 621-4438
Fax: / 001 520 621-4899
e-mail address: /
website: /
Name (including Title) of Head of Laboratory (Responsible Official): / Donald V. Lightner, Professor
Name(including Title and Position) of OIE Reference Expert: / Donald V. Lightner, Professor
Date of submission to the OIE / 25 January 2013

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:

Each ToR (blueitalicised text)has been placed as a heading covering the group of questions related to it.

Please note the reditalicisedtext is given as guidance and should be deleted from your report and substitute with your data. Examples are based onpast Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:

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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test recommended by the OIE / Total number of test performed last year
Indirect diagnostic tests / Nationally / Internationally
0 / 0 / 0
Direct diagnostic tests / Nationally / Internationally

Standard RT-PCR & nested RT-PCR

/ 533 / 345

Real-time R-T PCR

/ 0 / 56

ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

Tostore and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2.Did your laboratory produce or store imported standard reference reagentsofficially recognised by the OIE or other international bodies?

x Yes No

3.Did your laboratory supply standard reference reagentsto OIE Member Countries?

x Yes No

Type of reagent available / Related diagnostic test / Produced/
stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions
Control positive Infected tissue / RT-PCR / Produced / 1 tube / Madagascar
Control positive DNA plasmid / RT-PCR / Produced / 1 tube / Nicaragua
Control positive DNA plasmid / Realtime RT-PCR / Produced / 1 tube / Indonesia

4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

x Yes No

5.Did your laboratory produce vaccines?

Yesx No

6.Did your laboratory supply vaccines to OIE Member Countries?

Yesx No

ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

Yesx No

8.Didyour laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

Yesx No

ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?

x Yes No

Name of OIE Member Country seeking assistance / Date
(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
Saudi Arabia / 11 January / 2
Saudi Arabia / 19 January / 4
Saudi Arabia / 19 January / 1
Brazil / 23 January / 2
Brazil / 23 January / 4
Brazil / 23 January / 5
Brazil / 23 January / 16
Panama / 24 January / 1
Saudi Arabia / 25 January / 18
Belize / 25 January / 2
Belgium / 25 January / 1
South Africa / 1 February / 3
Saudi Arabia / 14 February / 20
India / 20 February / 4
Saudi Arabia / 29 February / 12
Brazil / 1 March / 14
Brazil / 1 March / 7
Brazil / 1 March / 12
New Caledonia / 2 March / 24
India / 21 March / 3
Norway / 22 March / 1
Belgium / 29 March / 1
New Caledonia / 19 April / 19
Belgium / 20 April / 1
Indonesia / 25 April / 6
Indonesia / 25 April / 6
Belgium / 16 May / 1
Belize / 30 May / 4
New Caledonia / 14 June / 1
New Caledonia / 13 July / 25
Saudi Arabia / 17 July / 30
Brazil / 17 July / 12
Belize / 31 July / 5
India / 1 August / 8
Belize / 6 August / 3
India / 1 August / 8
Saudi Arabia / 20 August / 40
India / 24 August / 3
Belgium / 29 August / 1
Brazil / 13 September / 12
Brazil / 14 September / 12
New Caledonia / 24 September / 1
Belgium / 15 October / 2
Saudi Arabia / 16 October / 16
India / 16 October / 2
Indonesia / 24 October / 3
Indonesia / 24 October / 6
India / 30 October / 6
Belgium / 13 November / 1
India / 26 November / 8
Belize / 3 December / 12
Panama / 4 December / 1
New Caledonia / 5 December / 10
Brazil / 5 December / 12

10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

X Yes No

Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was provided
Madagascar / Annual review of disease status / In loco

ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than your own?

Yesx No

ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12.Didyour Laboratory collect epizootiological data relevant to international disease control?

Yesx No

13.Didyour laboratory disseminate epizootiological data that had been processed and analysed?

Yesx No

14.What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a)Articles published in peer-reviewed journals:...... 3

b)International conferences:...... 2

c)National conferences:...... 0

d)Other:...... 0

Lightner DV, Redman RM, Pantoja CR, Tang KFJ, Noble BL, Schofield P, Mohney LL, Nunan LM, Navarro SA. 2012. Historic emergence, impact and current status of shrimp pathogens in the Americas. Journal of Invertebrate Pathology. 110:174-183.

Aranguren LF, Tang KFJ, Lightner DV. Protection from yellow head virus (YHV)

infection in Penaeus vannamei pre-infected with Taura syndrome virus (TSV). Dis. Aquat.Org. 98: 185–192.

Tang KF, Navarro SA, Pantoja CR, Aranguren LF, Lightner DV. 2012. New genotypes of white spot syndrome virus (WSSV) and Taura syndrome virus (TSV) from the Kingdom of Saudi Arabia. Dis. Aquat.Org. 99: 179–185.

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

X Yes No

a)Technical visits:...... 1

b)Seminars:...... 1

c)Hands-on training courses:...... 2

d)Internships (>1 month):...... 0

Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding country
a / Madagascar / 40
b / Mexico / 200
c / Nicaragua / 5
c / Brazil, Mexico, Vietnam, French Polynesia, the Netherlands, Indonesia, Thailand, Norway / 20

ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16.Does your laboratory have a Quality Management System certified according to an International Standard?

Yesx No

17.Is your laboratory accredited by an international accreditation body?

x Yes No

Test for which your laboratory is accredited / Accreditation body
RT-PCR and Histology for TS disease / USDA APHIS

18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 orManual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

x Yes No

ToR:To organise and participate in scientific meetings on behalf of the OIE

19.Did your laboratory organise scientific meetings on behalf of the OIE?

Yesx No

20.Did your laboratory participate in scientific meetings on behalf of the OIE?

Yesx No

ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

YesX No

22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

YesX No

23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yesx No

ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24.Did your laboratory organiseor participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

x Yes No

Purpose for inter-laboratory test comparisons[1] / No. participating laboratories / Participating OIE Member Countries
TS detection using RT-PCR / 36 / Belize, Colombia, Costa Rica, Ecuador, El Salvador, India, Madagascar, Mexico, Nicaragua, Saudi Arabia, Singapore, Thailand, Vietnam

ToR:To place expert consultants at the disposal of the OIE

25.Did your laboratory place expert consultants at the disposal of the OIE?

x Yes No

Kind of consultancy / Location / Subject (facultative )
Member expert team / Vietnam / Early Mortality Syndrome (EMS)/Acute Hepatopancreatic Necrosis Syndrome (AHPNS)

Annual reports of OIE Reference Laboratories, 20121

[1]See Interlaboratory test comparisons in: Laboratory Proficiency Testing at:
see point 1.3