OIE RL for «Infectious Salmon Anaemia» – «Birgit Helene Dannevig» – «Norway»
OIE Reference Laboratory Reports
Activities in 2012
Address of laboratory / Norwegian Veterinary Institute
Pb 750 Sentrum
N-0106 Oslo, Norway
Tel.: / +47 2321 6000
Fax: / +47 2321 6001
e-mail address: /
website: /
Name (including Title) of Head of Laboratory (Responsible Official): / Gudmund Holstad
Name(including Title and Position) of OIE Reference Expert: / Birgit Helene Dannevig (diseased), Ph.D., Researcher
A new expert, Dr. Knut Falk will be proposed to the OIE this spring.
Date of submission to the OIE / 30.01.13
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:
EachToR(blueitalicised text)has been placed as a heading covering the group of questions related to it.
Please note the reditalicisedtext is given as guidance and should be deleted from your report and substitute with your data. Examples are based onpast Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards
Test recommended by the OIE / Total number of test performed last yearIndirect diagnostic tests / Nationally / Internationally
Histopathology / 21
Direct diagnostic tests / Nationally / Internationally
Immunohistochemistry / 85
Cell culture/IFAT for virus isolation/identification
/ 10 / 1Real-time RT-PCR / 380 / 95
Sequencing for genotyping / 14
ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.
Tostore and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2.Did your laboratory produce or store imported standard reference reagentsofficially recognised by the OIE or other international bodies?
YesX No
3.Did your laboratory supply standard reference reagentsto OIE Member Countries?
X* YesNo
*We have ISAV positive material/isolates and ISAV susceptible cell lines in our laboratory, which we have supplied to laboratories in other countries. I am not aware of whether these are officially recognised by the OIE or other international bodies as standard reference reagents. We have still chosen to include this in the table below.
Type of reagent available / Related diagnostic test / Produced/stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions
ISAV reference strain / ISAV RT-PCR,
Isolation of ISAV in cell culture / Norway / 2x0.5ml / CISA-INIA,
Spain
ISAV reference strain / ISAV RT-PCR,
Isolation of ISAV in cell culture / Norway / 2x0.5 ml / Investigation and Diagnostic Centres and Response
Ministry for Primary Industries, Upper Hutt, New Zealand
ISAV reference strains / ISAV RT-PCR,
Isolation of ISAV in cell culture / Norway / 2x0,5ml / Norwegian College of Fishery Science,
University of Tromsø, Norway
ISAV susceptible ASK-2 cells / Isolation of ISAV in cell culture / Norway / 2 small cell flasks / Investigation and Diagnostic Centres and Response
Ministry for Primary Industries, Upper Hutt, New Zealand
ISAV susceptible SHK-1 cells / Isolation of ISAV in cell culture / Norway / 2 small cell flasks / Immunobiology Laboratory, Department of Zoology, University of Delhi, India
4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?
X** Yes No
**See table above in 3. for specification
5.Did your laboratory produce vaccines?
YesX No
6.Did your laboratory supply vaccines to OIE Member Countries?
YesX No
ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?
YesX No*
*We have previously developed methods for diagnosis of ISAV, but not within the last year.
8.Didyour laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
YesX No
ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?
X Yes No
Name of OIE Member Country seeking assistance / Date(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
New Zealand / 21 May / 119 / 0
10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
X Yes No
Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was providedNew Zealand / Development of ISA diagnostics / Training in our lab
ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?
X Yes No
Title of the study / Duration / Purpose of the study / Partners (Institutions) / OIE Member Countries involved other than your countryCharacterisation of ISA HPR0 virus- and infection in Atlantic salmon / 3 years / Increasing the knowledge of the significance of HPR0 ISAV / Food, Veterinary and Environmental Agency, Faroe Islands
Marine Scotland, Marine Laboratory, Aberdeen, UK / Denmark (Faroe Islands)
United Kingdom
Application of a new principle to combat infectious salmon anemia (ISA) / 3 years / To elucidate whether targeting viral antigens to salmon antigen presenting
cells can be used to
- dissect salmon immune responses important for protection against viruses
- develop an effective vaccine against ISA / Institut National de la Recherche Agronomique, Jouy-en-Josas, France
Syddansk Universitet,Odense C, Danmark / France
Denmark
ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
12.Didyour Laboratory collect epizootiologicaldata relevant to international disease control?
X Yes No
13.Didyour laboratory disseminate epizootiologicaldata that had been processed and analysed?
X Yes No
14.What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a)Articles published in peer-reviewed journals:...... 3
b)International conferences:...... 1
c)National conferences:...... 3
d)Other:...... 2
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?
X Yes No
a)Technical visits:...... 1
b)Seminars:...... 0
c)Hands-on training courses:...... 0
d)Internships (>1 month):...... 0
Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding countrya / New Zealand / 1
ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
16.Does your laboratory have a Quality Management System certified according to an International Standard?
X Yes No
Quality management system adoptedISO 17025
17.Is your laboratory accredited by an international accreditation body?
X* Yes No
*Accreditation of ISAV specific tests are in progress, expected finalised fall 2013. However, the laboratory has a related accredited PCR test for influenza A listed below.
Test for which your laboratory is accredited / Accreditation bodyInfluenza A virus (matrix) – Detection with real-time RT-PCR (ME07_127) / Norwegian Accreditation, member of EA
18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 orManual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
X Yes No
ToR:To organise and participate in scientific meetings on behalf of the OIE
19.Did your laboratory organise scientific meetings on behalf of the OIE?
YesX No
20.Did your laboratory participate in scientific meetings on behalf of the OIE?
YesX No
ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?
YesX No
22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?
YesX No
23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?
ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.
24.Did your laboratory organiseor participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?
X Yes No
Purpose for inter-laboratory test comparisons[1] / No. participating laboratories / Participating OIE Member CountriesThe EU-RL Annual Inter-laboratory Proficiency Test 2012 focused on identification of the pathogens causing the non-exotic and exotic fish diseases listed in Council Directive 2006/88/EC, including ISA / In 2011 41 NRL’s participated.
Report for 2012 not yet available / Arranged by European Union Reference Laboratory for Fish Diseases in Aarhus, Denmark
In 2011 all EU member states participated andalso NRLs from:
Australia, Bosnia and Herzegovina, Croatia, Faroe Islands, Iceland, Israel, Iran, Japan, Norway, P.R. China, Serbia, Switzerland, Turkey and USA
Inter-Laboratory Proficiency Test for Fish viruses - testing of Norwegian commercial laboratories / 4 / The OIE Reference laboratory for ISA, Norway, and 3 commercial laboratories
ToR:To place expert consultants at the disposal of the OIE
25.Did your laboratory place expert consultants at the disposal of the OIE?
X Yes No
Kind of consultancy / Location / Subject (facultative )OIE appointed expert panel / Canada / Audit of OIE Reference Laboratory for Infectious Salmon Anaemia at the Atlantic Veterinary College, Canada
On-going revision of the OIE Manual on ISA / Chapter 2.3.5 Aquatic Health Manual
List of publications, OIE Reference Laboratory of ISA, Norwegian Veterinary Institute, Oslo, Norway
a)
Lyngstad, TM, Kristoffersen AB, Hjortaas MJ, Devold M, Aspehaug V, Larsen RB, Jansen, PA, 2012. Low virulent infectious salmon anaemia virus (ISAV-HPR0) is prevalent and geographically structured in Norwegian salmon farming. Diseases of Aquatic Organisms,101:197-206, 2012
Aamelfot, M., Dale, O.B., Weli, S., Koppang, E.O., Falk, K. (2012) Expression of 4-O-acetylated sialic acids on Atlantic salmon endothelial cells correlates with cell tropism of Infectious salmon anemia virus. J.Virol. 86; 10571-10578.
Olsen, C.M., Braaen, S., Falk, K., Rimstad, E. (2012) Multiple passages of infectious salmon anemia virus in rainbow trout (Oncorhynchusmykiss) did not induce increase in virus load. J.FishDis. 35; 827-838.
b)
Lyngstad, TM, Kristoffersen AB, Hjortaas MJ, Devold M, Aspehaug V, Larsen RB, Jansen, PA, 2012. Phylogeographic analyses of low virulent infectious salmon anaemia (ISA) virus (HPR0) in farmed Atlantic salmon in Norway. Oral presentation.ISVEE 13 2012, 13th International symposium on veterinary epidemiology & economics, 19-24 August 2012.Maastricht, Netherlands
c)
Lyngstad TM, Kristoffersen AB, Hjortaas MJ, Devold M, Larssen RB og Jansen PA. ILA virus (HPRO) i norsk oppdrettslaks – Fylogeografi. Oral presentation. Programkonferansen Havbruk 2012
Markussen, T., Sindre, H., Tengs, T., Hjortaas, M.J., Dale, O.B., Christiansen, D.H., Falk, K. Pyrosekvensering og analyse av ILAV HPR0-positivt gjellemateriale fra Atlantisk Laks. Programkonferansen HAVBRUK 2012, Stavanger, 16.–18. april 2012
Austbø, L., Aas, I.B.,Falk, K., Kønig, M., Weli, S.C., Westad, E., Syed, M., Koppang, E.O. Gjeller og det interbrankialt lymfatiske vevet hos laks – dets rolle i virusinfeksjon. Programkonferansen HAVBRUK 2012, Stavanger, 16.–18.april 2012
d)
Thesis for the degree of Philosophiae Doctor "Tracing transmission pathways for infectious salmon anaemia (ISA) virus", Norwegian School of Veterinary, 2012
Marine Labs, Aberdeen, Scotland.Ultra-deep pyrosequencing of the variable gene regions encoding the hemagglutinin-esterase (HE) and fusion protein (F) from high-pathogenic and low-pathogenic (HPR0) strains of infectious salmon anaemia virus (ISAV).Turhan Markussen, Hilde Sindre, Torstein Tengs, Christine Monceyron Jonassen, Sanela Numanovic, Monika J. Hjortaas, Ole Bendik Dale, Debes H. Christiansen and Knut Falk.
Annual reports of OIE Reference Laboratories, 20121
see point 1.3