OIE RL for « Avian influenza » – « Dr. Ilaria Capua » – « Italy »
OIE Reference Laboratory Reports
Activities in 2012
Address of laboratory / OIE Reference Laboratory for Avian Influenza disease
Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Viale dell’Università 10 – 35020 Legnaro (PD) - Italy
Tel.: / +39-049-8084371
Fax: / +39-049-8084360
e-mail address: /
website: /
Name (including Title) of Head of Laboratory (Responsible Official): / Dr. Ilaria Capua
Director OIE Reference Laboratory for Avian Influenza and Newcastle disease
Name (including Title and Position) of OIE Reference Expert: / Director OIE Reference Laboratory for Avian Influenza and Newcastle disease
Date of submission to the OIE / 31 January 2012
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:
Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.
Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards
Test recommended by the OIE / Total number of test performed last yearIndirect diagnostic tests / Nationally / Internationally
c-ELISA (AI type A) / 1038 tests / 281 tests
AGID / 300 tests / 0 tests
HI (H5) / 27371 tests / 246 tests
HI (H7) / 25785 tests / 222 tests
Direct diagnostic tests / Nationally / Internationally
Virus isolation in SPF embryonated chicken eggs
/ 423 tests / 208 testsVirus isolation in cell culture (MDCK)
/ 29 tests / 87 testsReal-time RT-PCR gene M
/ 9213 tests / 1515 testsReal-time RT-PCR (H5-H7-H9)
/ 529 tests / 501 testsSequencing of AIV genes
/ 381 tests / 981 testsToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.
To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2.Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?
Yes No
3.Did your laboratory supply standard reference reagents to OIE Member Countries?
Yes No
Type of reagent available / Related diagnostic test / Produced/stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions
Positive reference antigen / HI / Produced / 2044 ml / Italy – IZS Public Health Institutes
Positive reference sera / HI / Produced / 249 ml / Italy – IZS Public Health Institutes
Negative reference sera / HI / Produced / 12 ml / Italy – IZS Public Health Institutes
Positive reference antigen / HI / Produced / 12 ml / France - CNRS-Delegation Languedoc
Positive reference sera / HI / Produced / 8ml / France - CNRS-Delegation Languedoc
Positive reference antigen / HI / Produced / 1 ml / Romania - Institute for Diagnosis and Animal Health
Positive reference sera / HI / Produced / 9 ml / Romania - Institute for Diagnosis and Animal Health
Positive reference antigen / HI / Produced / 9 ml / Tunisia- IRVT
Positive reference sera / HI / Produced / 16 ml / Tunisia- IRVT
Negative reference sera / HI / Produced / 1ml / Tunisia- IRVT
Negative reference sera / HI / Produced / 1ml / Algeria – CVL
Positive reference antigen / HI / Produced / 6 ml / Senegal - Pasteur Institute
Positive reference sera / HI / Produced / 6 ml / Senegal - Pasteur Institute
Positive reference antigen / HI / Produced / 21 ml / South Africa -Centre for Respiratory Diseases
Positive reference sera / HI / Produced / 6 ml / South Africa - Centre for Respiratory Diseases
Positive reference antigen / HI / Produced / 72 ml / Proficiency Test Africa 2012 - CVLs of African countries (Cameroun, Central African Republic, Congo, RD Congo, Ethiopia, Gabon, Nigeria, Senegal, Ruanda, Tanzania, Uganda, Botswana)
Positive reference sera / HI / Produced / 64 ml / Proficiency Test Africa 2012 (see above)
Negative reference sera / HI / Produced / 20 ml / Proficiency Test Africa 2012 (see above)
4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?
Yes No
5.Did your laboratory produce vaccines?
Yes No
6.Did your laboratory supply vaccines to OIE Member Countries?
Yes No
ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?
Yes No
8.Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
Yes No
ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?
Yes No
Name of OIE Member Country seeking assistance / Date(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
Saudi Arabia / 5 May / 34 / -
Bangladesh / 14 September / - / 20
Bulgaria / 20 March / - / 5
Cameroun / 30 August / 281 / -
Egypt / 7 August;
26 October / 192 / -
United Arab Emirates / 10 July / 6 / -
France / 3 May / - / 2
Jordan / 14 September / - / 9
Iraq / 17 April;
19 December / 41 / -
Malaysia / 4 April;
21 May / 164 / -
Poland / 17 February / - / 5
10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
Yes No
Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was providedIsrael / To analyze the current epidemiological status on Avian Influenza and Newcastle Disease in Israel and outcome future plans on quality assurance, development and reinforcement of diagnostic tools for the control of AI and ND. / Meeting with local CVO and heads of diagnostic laboratories during the EU TWINNINg PROJECT sivia “Strengthening of Israeli Veterinary Inspection Authorities for Animal Health and Livestock Production”
Egypt / To analyze and improve the vaccination strategy against the HPAI H5N1 epidemic in Egypt. / Meetings with local CVO and Faculties of Veterinary Medicine
Azerbaijan / Biosurveillance of Avian Influenza and Newcastle disease viruses within the Barda region of Azerbaijan / Provision of diagnostic protocols, reference reagents and expertise to the Azerbaijan Republican Veterinary Laboratory in Baku.
ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?
Yes No
Title of the study / Duration / Purpose of the study / Partners (Institutions) / OIE Member Countries involved other than your countryFP7-FLUPIG / 4 years / Pathogenesis and transmission of influenza in pigs. / Universiteit Gent, Istituto Zooprofilattico Sperimentale delle Venezie, Imperial College of Science, Technology and Medicine, The Secretary of State for Environment, Food and Rural Affairs, Philipps Universitaet Marburg, Erasmus Universitair Medisch Centrum, Panstwowy Instytut Weterynaryjny ‐ Panstwowy Instytut Badawczy, HKU ‐ Pasteur Research Centre Limited, Kansas State University, Friedrich Loeffler Institut – Bundesforschungsinstitut
Fuer Tiergesundheit / UK, Italy, Germany, Netherlands, Poland, Hong Kong, USA
FP7-ESNIP III / 3 years / European surveillance network for influenza in pigs / Veterinary Laboratories Agency, Ghent University, AFSSA- LERAPP, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emillia Romagna, Technical University of Denmark, National Veterinary Institute, Pulawy, Laboratorio Central Veterinario-Sanidad Animal,
IDT Biologika Gmbh, Finnish Food Safety Authority, Kimron Veterinary Institute, Central Agricultural Office Veterinary Diagnostic Directorate, Central Veterinary Institute of Wageningen UR, University of Thessaly, Sanger Institute, University of Cambridge, University of Oxford, Freidrich-Loeffler-Institut, Istituto Zooprofilattico Sperimentale delle Venezie,
St. Judes Research Hospital, United States Department of Agriculture, Harbin Veterinary Institute, Merial S.A.S – MSS, Laboraotorios Hipra, S.A, Animal Health Trust, Agri-Food & Biosciences Institute / UK, Belgium, France, Italy, Denmark, Poland, Spain, Germany, Finland, Israel,Hungary, Netherlands, USA, PR China
FP7-PREDEMICS / 5 years / Preparedness, Prediction and Prevention of Emerging Zoonotic Viruses with Pandemic Potential using Multidisciplinary Approaches / Institut Pasteur, Istituto Zooprofilattico Sperimentale delle Venezie,Katholieke Universiteit Leuven,
Université de la Méditerrannée Aix-Marseille 2, Eidgenössisches Volkswirtschaftsdepartment, Imperial College of Science, Technology and Medicine, Agence Nationale de Sécurité Sanitaire, Max Planck Gesellschaft zur Foerderung der Wissenschaften E.V., Fundación Caubet-Cimera,
Swedish Institute for Infectious Disease Control,
Philipps Universität Marburg, Istituto Nazionale Malattie Infettive Lazzaro Spallanzani, University of Edinburgh, Institute for Scientific Interchange Foundation, Health Sciences e Training Foundation / France, Italy, Belgium, Switzerland, UK, Germany, Sweden,
EFSA-FLURISK / 2 years / Development of a risk assessment methodological framework for potentially pandemic influenza strains / Istituto Zooprofilattico Sperimentale delle Venezie (coordinator), Veterinary Laboratories Agency, National Institute for Public Health and the Environment, Pasteur Institute, Ghent University / Italy, UK, Netherlands, France, Belgium
EMIDA ERA-Net Epi-Seq / 3 years / Molecular epidemiology of epizootic diseases using next generation sequencing technology / CODA-CERVA, Friedrich-Loeffler-Institut, Swedish University of Agricultural Sciences, Institute for Animal Health, Istituto Zooprofilattico Sperimentale delle Venezie, University of Glasgow / Belgium, Germany, Sweden, UK
OIE Twinning / 2 years / Implementation of capacity building of the CVL for the early detection and diagnosis of AI and ND and other major viral diseases of poultry / IZSVe, CVL-IVO / Italy, Iran
ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
12.Did your Laboratory collect epizootiological data relevant to international disease control?
Yes No
13.Did your laboratory disseminate epizootiological data that had been processed and analysed?
Yes No
14.What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals:...... 13
b) International conferences:...... 16
c) National conferences:...... 2
d) Other:...... -
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?
Yes No
a) Technical visits:...... 5
b) Seminars:...... 2
c) Hands-on training courses:...... 7
d) Internships (>1 month):...... 6
Type of technical training provided (a, b, c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding countryd / Italy / 5
a / South Korea / 4
a / Poland / 1
d / Nigeria / 1
b-c / Angola / 2
c / Italy / 2
c / Saudi Arabia / 2
c / Iraq / 1
ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
16.Does your laboratory have a Quality Management System certified according to an International Standard?
Yes No
Quality management system adoptedISO 17025
17.Is your laboratory accredited by an international accreditation body?
Yes No
Test for which your laboratory is accredited / Accreditation bodyHI
AGID
Virus isolation in embryonated eggs / Accredia
18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)
Yes No
ToR:To organise and participate in scientific meetings on behalf of the OIE
19.Did your laboratory organise scientific meetings on behalf of the OIE?
Yes No
20.Did your laboratory participate in scientific meetings on behalf of the OIE?
Yes No
Title of event / Date(mm/yy) / Location / Role (speaker, presenting poster, short communications) / Title of the work presented
WHO Vaccine Composition Meeting / 02/12 / Geneva WHO / Speaker / OFFLU project
WHO Vaccine Composition Meeting / 09/12 / Beijing, PR China / Speaker / OFFLU project
ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?
Yes No
- Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?
Yes No
Purpose of the proficiency tests: (validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.) / Role of your Reference Laboratory (organiser/ participant) / No. participants / Participating OIE Ref. Labs/ organising OIE Ref. Lab.European Proficiency Test on AI and ND / Participant / Information available from organiser (AHVLA, UK) / Information available from organiser (AHVLA, UK)
- Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?
Yes No
Title of the project or contact / Scope / Name(s) of relevant OIE Reference LaboratoriesEMIDA ERA-Net Epi-Seq / Molecular epidemiology of epizootic diseases using next generation sequencing technology / Friedrich-Loeffler-Institut
ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.
- Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?
Yes No
Purpose for inter-laboratory test comparisons[1] / No. participating laboratories / Participating OIE Member CountriesProficiency test for AI and Newcastle disease (organiser) / 12 / Cameroun, Central African Republic, Congo, Ethiopia, Gabon, Nigeria, Senegal, Ruanda, Tanzania, Uganda, Botswana
Proficiency test for AI and Newcastle disease (organiser) / 15 / Italy (Italian laboratories belonging to the network of the laboratories participating to the national surveillance for AI)
ToR: To place expert consultants at the disposal of the OIE
25.Did your laboratory place expert consultants at the disposal of the OIE?
Yes No
List of publications of Reference Laboratory (Italy) in 2012 – Avian Influenza
- Capua, I. (2012). Restore ties lost in the arab spring. Nature, 483, 536.
- Capua, I., & Alexander, D. J. (2012) (edited). Control of influenza in animal, in Textbook of Influenza, Wiley, London.
- Capua I., Mercalli A., Pizzuto M. S., Romero-Tejeda A., Kasloff S., De Battisti C., Bonfante F., Patrono L., Vicenzi E., Zappulli V., Lampasona V., Stefani A., Doglioni C., Terregino C., Cattoli G., Piemonti L. (2012). Influenza A viruses grow in human pancreatic cells and cause pancreatitis and diabetes in an animal model. Journal of Virology, epub.
- Dundon, W. G. (2012). Variability among the neuraminidase, non-structural 1 and PB1-F2 proteins in the influenza virus genome. Virus Genes, 44, 363-373.
- Dundon, W. G., Heidari, A., Fusaro, A., Monne, I., Beato, M. S., Cattoli, G., et al. (2012). Genetic data from avian influenza and avian paramyxoviruses generated by the European network of excellence (EPIZONE) between 2006 and 2011 - review and recommendations for surveillance. Veterinary Microbiology, 154(3-4), 209-221.
- Fouchier, R. A., García-Sastre, A., Kawaoka, Y., Barclay, W. S., Bouvier, N. M., Brown, I. H., et al. (2012). Pause on avian flu transmission research. Science, 335(6067), 400-401.
- Fouchier, R. A., García-Sastre, A., Kawaoka, Y., Barclay, W. S., Bouvier, N. M., Brown, I. H., Capua I., et al. (2012). Pause on avian flu transmission studies. Nature, 481(7382), 443.
- Gambaryan, A. S., Matrosovich, T. Y., Philipp, J., Munster, V. J., Fouchier, R. A., Cattoli, G., et al. (2012). Receptor-binding profiles of H7 subtype influenza viruses in different host species. Journal of Virology, 86(8), 4370-4379.
- Kongkamnerd, J., Cappelletti, L., Prandi, A., Seneci, P., Rungrotmongkol, T., Jongaroonngamsang, N., Rojsitthisak P, Frecer V, Milani A, Cattoli G, Terregino C, Capua I., et al. (2012). Synthesis and in vitro study of novel neuraminidase inhibitors against avian influenza virus. Bioorganic & Medicinal Chemistry, 20(6), 2152-2157.
- Kongkamnerd, J., Milani, A., Cattoli, G., Terregino, C., Capua, I., Beneduce, L., et al. (2012). A screening assay for neuraminidase inhibitors using neuraminidases N1 and N3 from a baculovirus expression system. Journal of Enzyme Inhibition and Medicinal Chemistry, 27(1), 5-11.
- Monne, I., Hussein, A. H., Fusaro, A., Valastro, V., Hamoud, M. M., Khalefa, R. A., et al. (2012). H9N2 influenza A virus cocirculates in H5N1 endemically infected poultry population in Egypt. Influenza and Other Respiratory Viruses, 6(4).
- Njaabo, K. Y., Fuller, T. L., Chasar, A., Pollinger, J. P., Cattoli, G., Terregino, C., et al. (2012). Pandemic A/H1N1/2009 influenza virus in swine, Cameroon, 2010. Veterinary Microbiology, 156(1-2), 189-192.
- Pérez, L. J., Dìaz de Arce, H., Cilloni, F., Salviato, A., Marciano, S., Perera, C. L., et al. (2012). An SYBR green-based real-time RT-PCR assay for the detection of H5 haemagglutinin subtype avian influenza virus. Molecular and Cellular Probes, 26(3), 137-145.
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Annual reports of OIE Reference Laboratories, 20121