Additional File 3: Identification of the 67 contigs that were differentially expressed by the BAT 477 (37 contigs) and Pérola genotypes (30), which are tolerant and susceptible to water deficit, respectively, during both investigated developmental stages. Data include contig identification, derived gene products from the GenBank database, species with the highest similarityestimates and corresponding E-values, and the number of times the EST was sampled.

Contigs / Genic product description / Species
/GI number / E-value / *Number of reads
Pérola – Flowering
CL11Contig1 / protein kinase / Phaseolus vulgaris
/166788445 / 3E-79 / 27
CL17Contig1 / hypothetical protein MTR_5g051130 / Medicago truncatula
/357488213 / 4E-39 / 2
CL1Contig100 / S-adenosyl methionine synthase / Phaseolus lunatus
/18157331 / 1E-67 / 11
CL1Contig105 / AMT1;1 ammonium/proton co-transporter / Phaseolus vulgaris
/300119971 / 1E-48 / 2
CL1Contig120 / chloroplast photosystem II 10 kDa protein / Arachis hypogaea
/83776786 / 2E-45 / 13
CL1Contig122 / unknow protein / Oryza sativa
/55168340 / 2E-13 / 2
CL1Contig96 / PREDICTED: chlorophyll a-b binding protein 3, chloroplastic-like / Glycine max
/356532722 / 7E-52 / 2
CL20Contig1 / E2 ubiquitin-conjugating enzyme UBC10 / Brassica napus
/183013548 / 7E-56 / 13
CL2Contig1 / PREDICTED: ethylene-responsive transcription factor 3-like / Glycine max
/356552847 / 3E-22 / 2
CL31Contig1 / cysteine proteinase / Betula pendula
/16076439 / 6E-44 / 9
CL35Contig1 / protein phosphatase 2C (PP2C) / Fagus sylvatica
/7768151 / 1E-36 / 5
CL37Contig1 / hypothetical protein At3g27750 / Arabidopsis thaliana
/49660179 / 4E-15 / 6
CL41Contig1 / PREDICTED: 17.9 kDa class II heat shock protein-like / Glycine max
/356507036 / 9E-64 / 4
CL45Contig1 / PREDICTED: chlorophyll a-b binding protein 3, chloroplastic-like / Glycine max
/356501755 / 3E-61 / 4
CL47Contig1 / class I chitinase / Phaseolus vulgaris
/52548196 / 6E-70 / 3
CL48Contig1 / Quinone oxidoreductase PIG3 / Medicago truncatula
/357471439 / 5E-97 / 3
CL49Contig1 / chaperone / Agave tequilana
/99033707 / 1E-07 / 3
CL50Contig1 / NADPH quinoneoxido reductase-like protein / Solanum tuberosum
/76160992 / 6E-61 / 3
CL56Contig1 / putative cysteine protease / Glycine max
/149393486 / 2E-52 / 3
CL5Contig1 / transcription factor C2H2 / Lotus japonicus
/193237563 / 1E-19 / 3
CL60Contig1 / Triacylglycerol lipase 1 precursor, putative / Ricinus communis
/223542048 / 1E-07 / 2
CL6Contig2 / dehydration responsive element binding protein / Glycine max
/351724471 / 9E-40 / 40
BAT 477 – Flowering
CL14Contig1 / hypersensitive induced reaction protein 1 / Glycine max
/354683205 / 6E-55 / 21
CL1Contig124 / Chlorophyll a-b binding protein 3C-like protein / Medicago truncatula
/357497757 / 2E-23 / 3
CL1Contig125 / PREDICTED: 40S ribosomal protein S17-4 / Glycine max
/356521554 / 4E-44 / 37
CL1Contig126 / PREDICTED: chlorophyll a-b binding protein 3, chloroplastic-like / Glycine max
/356532722 / 2E-35 / 2
CL1Contig127 / invertase/pectin methylesterase inhibitor family protein / Arabidopsis lyrata
/297310623 / 2E-06 / 23
CL1Contig128 / Auxin-repressed protein / Medicago truncatula
/357446689 / 2E-22 / 26
CL1Contig130 / calmodulin 24-like protein / Eperua grandiflora
/289065001 / 2E-26 / 2
CL1Contig137 / PREDICTED: 21 kDa protein-like / Glycine max
/356545276 / 5E-27 / 2
CL1Contig138 / PREDICTED: chlorophyll a-b binding protein 3, chloroplastic-like / Glycine max
/356532722 / 9E-30 / 2
CL1Contig47 / Pectinesterase inhibitor / Medicago truncatula
/357479079 / 2E-13 / 3
CL1Contig48 / Photosystem I reaction center subunit IV A / Medicago truncatula
/357510347 / 3E-10 / 3
CL1Contig62 / PREDICTED: 40S ribosomal protein S16-like / Glycine max
/356524632 / 1E-46 / 3
CL1Contig80 / NAD-dependent isocitratede hydrogenase / Nicotiana tabacum
/3790188 / 3E-37 / 2
CL21Contig1 / chlorophyll a/b binding protein type II / Glycine max
/16805332 / 5E-65 / 14
CL34Contig1 / Oxygen-evolving enhancer protein / Medicago truncatula
/358344003 / 2E-60 / 6
CL38Contig1 / uncharacterized protein LOC100305788 / Glycine max
/351721030 / 3E-32 / 5
CL46Contig1 / nodulin-26 / Glycine max
/310576 / 2,E-26 / 3
CL51Contig1 / S-adenosyl methionine synthase / Phaseolus lunatus
/18157331 / 9E-45 / 3
CL55Contig1 / NADH-ubiquinoneoxidoreductase-likeprotein / Camellia sinensis
/166012639 / 2E-32 / 3
CL57Contig1 / unknown / Medicago truncatula
/388520683 / 3E-25 / 2
Pérola - Grain filling
CL1Contig108 / unknown / Glycine max
/255632628 / 7E-56 / 2
CL1Contig11 / hypothetical protein MTR_8g040260 / Medicago truncatula
/357515297 / 5E-18 / 3
CL1Contig16 / Proteasome subunit alpha type / Medicago truncatula
/357517981 / 3E-45 / 14
CL1Contig17 / Ethylene-responsive transcription factor / Medicago truncatula
/357481437 / 1E-26 / 99
CL1Contig2 / PREDICTED: protein BRICK 1-like isoform 1 / Glycine max
/356496693 / 7E-42 / 205
CL1Contig24 / PREDICTED: imidazole glycerol phosphate synthase hisHF, chloroplastic-like / Glycine max
/356567431 / 3E-87 / 28
CL1Contig35 / unknown / Glycine max
/255626643 / 4E-34 / 3
CL2Contig2 / PREDICTED: ethylene-responsive transcription factor 7-like / Glycine max
/356501564 / 5E-11 / 2
BAT 477 - Grain filling
CL1Contig118 / chloroplast chlorophyll a/b binding protein / Lycoris radiata
/285804077 / 4E-24 / 2
CL1Contig57 / PREDICTED: chlorophyll a-b binding protein CP29.2, chloroplastic-like / Glycine max
/356496185 / 2E-13 / 2
CL1Contig58 / PREDICTED: 40S ribosomal protein S16-like / Glycine max
/356524632 / 9E-35 / 28
CL1Contig60 / Leucine zipper protein / Medicago truncatula
/357491217 / 2E-24 / 22
CL1Contig63 / PREDICTED: proteasome subunit alpha type-7-like isoform 1 / Glycine max
/356502736 / 9E-36 / 2
CL1Contig64 / unknown / Lotus japonicus
/388517649 / 3E-34 / 15
CL1Contig70 / Pectinesterase inhibitor / Medicago truncatula
/357479053 / 1E-18 / 2
CL1Contig75 / PREDICTED: cell wall / vacuolar inhibitor of fructosidase 1-like / Cicer arietinum
/502150782 / 3E-13 / 8
CL1Contig77 / unknown / Glycine max
/255638239 / 1E-06 / 2
CL1Contig79 / RW1-like protein / Arachis hypogaea
/83776790 / 7E-15 / 3
CL1Contig80 / NAD-dependent isocitrate dehydrogenase / Nicotiana tabacum
/3790188 / 3E-37 / 12
CL1Contig84 / Pectinesterase inhibito / Medicago truncatula
/357479053 / 6E-33 / 2
CL1Contig89 / unknown / Medicago truncatula
/388521273 / 7E-13 / 2
CL1Contig91 / PSI reaction center subunit III / Citrus sinensis
/157678952 / 8E-06 / 2
CL28Contig1 / PREDICTED: RING-H2 finger protein ATL66-like / Glycine max
/356539989 / 2E-34 / 9
CL34Contig1 / PREDICTED: oxygen-evolving enhancer protein 1, chloroplastic-like / Glycine max
/356559442 / 4E-61 / 2
CL42Contig1 / PREDICTED: chlorophyll a-b binding protein P4, chloroplastic-like / Glycine max
/356516812 / 5E-57 / 4

* Number ofdifferentially expressedEST's composing eachcontig, in whichidentical sequencesofESTswith redundancy≥5x are equivalentto auniquecontig.