sTable 1
Nra transcriptome: exponential phase
Differentially expressed genes in the nra mutant as determined by whole genome array hybridization and quantitative RT-PCR.
Mean intensity ratio of mutant/wildtypeb
ORFaGeneGene DescriptionRT-PCRc Exp1 Exp2 Average
Increased transcript abundance
spy0024purCphosphoribosylaminoimidazole-succinocarboxamide synthase3.764.944.35N.T.
spy0028purNPhosphoribosylglycinamide formyltransferase2.175.094.22N.T.
-nranegative regulatory protein Nra4.135.925.03 3.94 ± 6.77
-cpacollagen binding protein3.716.435.07 8.42 ± 6.54
spy0127-putative signal peptidase I3.525.394.45N.T.
spy0128-hypothetical protein2.964.623.79N.T.
spy0129srtCSortase C2.964.623.79N.T.
-prtF2fibronectin binding protein, Protein F24.662.423.54N.T.
spy0136-hypothetical protein2.207.594.89N.T.
spy0165nganicotine adenine dinucleotide glycohydrolase precursor10.7415.3213.03N.T.
spy0166-nicotine adenine dinucleotide glycohydrolase inhibitor10.8716.3813.62N.T.
spy0167slostreptolysin O precursor7.9514.2511.10 5.98 ± 7.68
spy0170-hypothetical protein4.1310.117.12N.T.
spy0216ralp3putative regulatory protein - RofA related2.003.762.88N.T.
spy0378hlyXputative hemolysin2.192.092.14N.T.
spy0416-subtilisin-like serine proteinase5.416.756.08N.T.
spy0469-putative 42 kda protein3.535.814.67N.T.
spy0488-hypothetical protein2.327.945.13N.T.
spy0489-hypothetical protein2.145.273.71N.T.
spy0600-conserved hypothetical protein2.015.023.52N.T.
spy0636idhOputative 5-keto-d-gluconate 5-reductase2.763.333.05N.T.
spy0638kdgKputative 2-keto-3-deoxygluconate kinase2.132.182.16N.T.
spy0705-hypothetical protein, phage associated31.9320.8626.39N.T.
spy0710-conserved hypothetical protein, phage associated3.324.914.11N.T.
spy0735ralp4putative regulatory protein - RofA related5.496.055.77 5.91 ± 8.17
spy0940-hypothetical protein2.537.304.92N.T.
spy0946-putative P1-type antirepressor, phage associated3.415.324.37N.T.
spy0952-hypothetical protein9.687.458.56N.T.
spy0997hylP2hyaluronidase, phage associated2.772.612.69N.T.
spy0999-hypothetical protein31.0331.1031.06N.T.
spy1134-putative ABC transporter2.212.992.60N.T.
spy1138-putative thiamine biosynthesis lipoprotein precursor2.123.392.76N.T.
spy1151ldhputative L-lactate dehydrogenase2.195.914.05N.T.
spy1189citFputative citrate lyase, alpha subunit4.092.503.30N.T.
spy1190-conserved hypothetical protein4.002.533.26N.T.
spy1198-putative repressor protein2.188.155.17N.T.
spy1280glmSputative L-glutamine-D-fructose-6-phosphate amidotransferase3.596.204.89N.T.
spy1419-putative repressor protein2.092.862.48N.T.
spy1443-hypothetical protein, phage associated4.366.865.61N.T.
spy1445hylP3hyaluronidase, phage associated6.402.204.30N.T.
spy1461-hypothetical protein2.632.212.42N.T.
spy1541arcCputative carbamate kinase2.672.432.55N.T.
spy1606-putative RNA methyltransferase7.205.116.15N.T.
spy1711-putative PTS system, enzyme IIA component2.072.782.43N.T.
spy1783-hypothetical protein2.9410.236.59N.T.
spy1956-hypothetical protein3.933.153.54N.T.
spy1983sclAcollagen-like surface protein, SclA6.6015.1410.87 3.32 ± 1.70
spy2010scpAC5a peptidase precursor4.455.785.11 2.54 ± 2.13
spy2018emmM protein precursor6.564.965.76N.T.
spy2019mgapositive regulatory protein Mga2.789.396.09N.T.
-sofSerum opacity factor precursor10.839.5010.17 3.22 ± 4.36
-sfbXfibronectin binding protein SfbX precursor20.303.6611.98N.T.
spy2122int4putative integrase19.4544.6032.03N.T.
spy2129-hypothetical protein2.903.563.23N.T.
spy2132-hypothetical protein2.092.382.24N.T.
spy2200hasAhyaluronate synthase44.6121.0532.83 22.23 ± 22.52
spy2201hasBUDP-glucose 6-dehydrogenase68.15121.0694.61 85.25 ± 81.67
spy2202hasCUDP-glucose pyrophosphorylase42.8415.8329.33N.T.
spyM18_0030purHphosphoribosylaminoimidazolecarboxamide formyltransferase2.162.762.46N.T.
spyM18_0339-orf122 phage protein23.9274.4849.20N.T.
spyM18_0348-putative DNA polymerase III delta prime subunit3.352.532.94N.T.
spyM18_0351-hypothetical protein9.7610.9810.37N.T.
spyM18_0358-hypothetical protein5.194.584.89N.T.
spyM18_0388-hypothetical protein, phage associated2.717.745.23N.T.
spyM18_0393speAstreptococcal pyrogenic exotoxin A precursor13.8412.8613.35 2.70 ± 4.24
spyM18_0453-hypothetical protein, phage associated2.717.745.23N.T.
M28_spy1450-putative esterase2.664.103.38N.T.
Reduced transcript abundance
spy0003dnaNbeta subunit of DNA polymerase III0.480.320.40N.T.
spy0021recODNA repair protein RecO0.390.140.26N.T.
spy0035abiRpossible abortive infection phage resistance protein0.450.310.38N.T.
spy0049rplC50S ribosomal protein L30.450.410.43N.T.
spy0077rpsM30S ribosomal protein S130.360.300.33N.T.
spy0078rpsK30S ribosomal protein S110.490.270.38N.T.
spy0183opuAAputative glycine betaine/proline abc transporter0.180.140.16N.T.
spy0184opuABCputative glycine-betaine binding permease protein0.170.370.27N.T.
spy0294oppBoligopeptidepermease0.500.450.48N.T.
spy0384fhuB.1putative ferrichrome ABC transporter0.490.460.48N.T.
spy0517thrSputative threonyl-tRna synthetase0.480.490.48N.T.
spy0657-putative cI-like repressor, phage associated0.480.290.39N.T.
spy0693-putative minor capsid protein, phage associated0.470.420.45N.T.
spy0703-hypothetical protein, phage associated0.480.320.40N.T.
spy0712mf2putative DNase, phage associated0.440.200.32N.T.
spy0742-hypothetical protein, phage associated0.500.270.39N.T.
spy0840rpsP30S ribosomal protein S160.490.260.38N.T.
spy0857mur1.2putative peptidoglycan hydrolase0.350.360.36N.T.
spy0870fmsputative polypeptide deformylase0.440.470.46N.T.
spy0913-putative ribosomal protein S10.500.120.31N.T.
spy0957-hypothetical protein - phage associated0.270.170.22N.T.
spy0963-hypothetical protein0.240.040.14N.T.
spy0967-conserved hypothetical protein - phage associated0.330.160.25N.T.
spy0968-hypothetical protein0.270.500.38N.T.
spy1046-conserved hypothetical protein0.120.010.07N.T.
spy1047-hypothetical protein0.230.040.13N.T.
spy1048-hypothetical protein0.200.020.11N.T.
spy1049-hypothetical protein0.140.100.12N.T.
spy1052-nucleoside diphosphate kinase0.210.210.21N.T.
spy1264-hypothetical protein0.400.270.33N.T.
spy1673-hypothetical protein0.390.330.36N.T.
spy1674-putative ABC transporter0.350.060.21N.T.
spy1675-conserved hypothetical protein0.250.180.21N.T.
spy1830-putative single strand binding protein0.440.290.37N.T.
spy1979skastreptokinase A precursor0.170.270.22 0.29 ± 0.18
spy2215-conserved hypothetical protein0.480.350.41N.T
spyM18_0345-hypothetical protein0.490.440.46N.T.
spyM18_0365-hypothetical protein0.430.410.42N.T.
spyM18_0375-hypothetical protein0.380.330.35N.T.
spyM18_0380-hypothetical protein0.400.450.42N.T.
spyM18_0382-hypothetical protein0.450.340.40N.T.
spyM18_0737-hypothetical protein, phage associated0.260.130.19N.T.
spyM18_0759-hypothetical protein0.470.410.44N.T.
spyM18_0765-hypothetical protein0.500.430.46N.T.
spyM18_1495-putative recombinase - phage associated0.480.290.38N.T.
spyM18_1798-hypothetical protein, phage associated0.170.360.26N.T.
spyM18_1805-putative phage repressor protein0.210.060.13N.T.
spyM3_1314-hypothetical protein0.480.220.35N.T.
spyM3_1321-conserved hypothetical protein0.400.310.36N.T.
spyM3_1323-hypothetical protein0.250.150.20N.T.
M28_spy0994-phage protein0.300.430.37N.T.
M28_spy1864-DNA integration/recombination/invertion protein0.050.000.02N.T.
M5005_spy1042-phage replication protein0.370.390.38N.T.
a ORFs listed according to spy, spyM3_, spyM18_, M28_ spy and M5005_spy numbers for GAS serotype M1 strain SF370, M3 strain MGAS315, M18 strain MGAS8232, M28 strain MGAS6180 and M1 strain MGAS5005 respectively.
b Mutant/wildtype intensity ratios as determined by microarray experiments. Exp1 and Exp2 are two independent experiments. Genes with > twofold changes in both experiments are shown.
c Mutant/wildtype transcript expression ratio determined by real-time RT-PCR from cells grown to exponential phase. Three independent total RNA were used for transcript analyses. Transcripts were normalized relative to gyrA transcript expression and are represented as mean expression ratio ± SD. N.T., Not tested.