Supplemental Material 1
For tables S1-S11:
Column 1: Experimentally identified snoRNAs are indicated by Oa and a contig number. Computationally found snoRNAs are labeled bOa followed by a common name or the contig number of a corresponding paralog (label i).
Column 2: The number of sequence clones presented in the cDNA library.
Column 3: Sanger genomic location.
Column 4: Genbank accession-number and CD-box snoRNA full-length sequences. Capital letters represent sequences from the cDNA library. Lower case letters represent genomic BLAST sequences. cDNAs are extended to presumed full-length.
Column 5: Human orthologs.
Column 6: Human GenBank accession numbers.
Column 7: Human ortholog C/D-box snoRNA.
----- denotes boxC and C'
----- denotes box D and D'
----- denotes stem
Table S 1: Full-length C/D box snoRNAs in introns of ribosomal protein genes (group I: conserved in vertebrates)
Ornithorhynchus anatinus / Homo sapiensname / numb. of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1707 / 23 / chr.11
5855481-5855557(-) / EU094058:
TCAAATGATGACACATTCTCCGGAATCTCTGTACTGCCTCTGATGAAAGTATAATTGAACCCTTTTCCATCTGATGA / C/D box 68 / NR_002450
chr.16 / CGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACATTTGAACCCTTTTCCATCTGATT
Oa1735a / 58 / ultra520
1653851-1653927(+) / EU094059:
tGTACATGATGATAGCTTGGCTCCCTCTACTGAAAATGGTGAGGATAATAGCCATGTCACCCAATAGTATCTGAACg / C/D box 100 / NR_002435
chr.6 / GCTGTACATGATGACAACTGGCTCCCTCTACTGAACTGCCATGAGGAAACTGCCATGTCACCCTTCTGATTACAGC
Oa1785 / 1 / contig14108
20860-20940(+) / EU094060:
gggtctgtgatgatgactttcactttATTTCGAGTCTCACGACCTTGAGATCA / C/D box 99 / NR_000020
chr.19 / GGCCGGTGATGAGAACTTCTCCCACTCACATTCGAGTTTCCCGACCATGAGATGACTCCACATGCACTACCATCTGAGGCCAC
Oa1817 / 3 / ultra497
343854-343916(-) / EU094061:
GTCAATGATGGAAAACTTTCCTGGCTGAGAACAGTGATGTTGTCAAAGGAACCACTGATGCAC / C/D box 42B / NR_000013
chr.17 / GTGCATATGATGGAAAAGTTTTAATCTCCTGACACTTGTGATGTCTTCAAAGGAACCACTGATGCAC
Oa1821 / 1 / contig21556
1584-1686(-) / EU094062:
cggtaatgatgaaattCCACCTCATTGGTCCGTGTTTCTGAGCCACATGATTTGTTCTGGAAGTTCTGACACtg / C/D box 18A / NR_002441
chr.15 / CAGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGATTTTCTCGGATGTTCTGATG
Oa1835 / 8 / contig21556
1585-1686(-) / EU094063:
TTGCCATGATGTCGTAATTTGCGTCTTACTCTGTTCTCAGTGACAGTTGCCTGCTGTCAAGAAGCTGGTACAGAAGGTTGACGAAAAGAAATCTCACTGAGCT / C/D box 16 / NR_002440
chr.15 / TGCAATGATGTCGTAATTTGCGTCTTACTCTGTTCTCAGCGACAGTTGCCTGCTGTCAGTAAGCTGGTACAGAAGGTTGACGAAAATTCTTACTGAGCAA
Oa1853 / 1 / contig16366
6448-6511(+) / EU094064:
TGGCGGTGATGACATTCTTAGGACACCTTTGGATTAACAATGAAAACAATCATCGACTGAGCCA
Oa1857 / 9 / contig4527
32917-33003 / EU094065:
GTTCAGTGATGAGGCACTGGAAAAAGCGCCGGGCACGGCGCCCGACCCCGATGAGGAATCCTTCCTTGCTGCCTTCCTTCTGAGAAC / C/D box 83A / NR_000027
chr.22 / GCTGTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAGCGCCCGAATCGGACAGTGTAGAACCATTCTCTACTGCCTTCCTTCTGAGAACAGC
Oa1942 / 4 / contig4527
32917-33003 / EU094066:
GTTCAGTGATGAGTCGCTGGAAGAAGCGCCGGGCACGGCGCCCGAAACGAATGAGGATACTCTCCTTGCTGCCTTCCTTCTGAGAACG / C/D box 83B / NR_000028
chr.22 / GCTGTTCAGTGATGAGGCCTGGAATGTGCGCTGGGCACAGCGCCCGAGACAGACTGCGGAACCGTTCCTTGTTGCCTTCCTTCTGAGAACAGC
Oa1990 / 4 / contig6062
27473-27543(-)
1 mm
A in cDNA seq
G in genomic seq / EU094067:
TATCGATGATGTACTCGAAAAAAAATGTCTGAACATATGAGTCCGCTAGTGATTTCAACGCACACTGAGAT / C/D box 72 / NR_002583
chr.5 / AGCTTATCAGTGATGTTGTAAAAATAAATGTCTGAACATATGAATGCAGTATTGATTTCAGCATTTAACTGAGATAAGCG
Oa2016 / 1773 / contig11099
20583-20654(+) / EU094068:
TGCAGTGATGTAAGAATTTCTTCACCTGAAAGAACCGTGTCGATCGAACGTACGAGCTTTTTAACCCTGAGC / C/D box 36B / NR_000017
chr.9 / GTTGCAGTGATGTAAAATTTCTTGGCCTGAAATTACTGTGAAGAGTAAAACCGAGCTTTTTAACACTGAGT
Oa2046 / 1 / contig1636
6082-6147(+) / EU094069:
TTGCTGTGATGACATTCTTAGGACACCTTTGGATTAATCGTGAAAATAAATTTCTATTCTGAGCAAC / new C/D / NT_010966
28506170-28506107
chr.18 / TTTCTGGTTGCTGTAATGACAGGACACCTTGGATTGATGGTGAAATAAACCATATCCTAAGAAA
Oa2073 / 3 / no 100% match in Platypus-BLAST / EU094070:
CCAAGTGATGAGACCTTTTGTACAGTTCTGCTGCTGATGTTGAGCGATGACAACGGGTAATCTGAAAC / C/D box 38b / NR_001457
chr.1 / TCTCAGTGATGAAAACTTTGTCCAGTTCTGCTACTGACAGTAAGTGAAGATAAAGTGTGTCTGAGGAGA
Oa2101 / 2 / contig11099
20947-21032(+) / EU094071:
tggcttTTGCAATGATGTATGAATTTCTTCACCTGAAAAAGAATGAAGAGCAAATACACGAGCTTTTTAACCCTGAGCAAAggcca / C/D box 36A / NR_002448
chr.9 / TTGCAATGATGTGAATCTCTCACTGAATTCAACCTTGAAGTGCGAATCCATGAGCTTTTTAACCCTGAGCAA
Oa2121 / 3 / contig14108
27792-27873(+) / EU094072:
GCAGATGATGTCCATTCTCACGATGGTCTTCAGATTGCCCCCCGTGGGCCCTGATGACACCGCCGAAAGGCTCAGCTGATGC / C/D box 35B / NR_001285
chr.19 / TGGCAGATGATGTTTGTTTTCACGATGGTCTTCAGATGCCCACGTGGGCACTGCTGAGAAAGCCACTTGGTAAAACTGATGCCGGAAA
Oa2126 / 27 / ultra497
338094-338166(-)
1 mm
C in cDNA seq
T in genomic seq. / EU094073:
cggATGCAAATGATGAACCGTAGTGACCAAAGCCTGAACCTTGGTGATGAAGCTTGACCACGTGATGCATCcg / C/D box 4B / NR_000009
chr.17 / GGTGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGTCATAATTAACTGACTGCACC
Oa2132 / 13 / ultra497
339354-339428(-) / EU094074:
cagggTGCAGATGATGACATCACAGCGACCAAAGCCTGAGTAAATGATTGCAACCTCACTGGCTGATGCAccctg / C/D box 4A / NR_000010
chr.17 / GGTGCAGATGATGACACTGTAAAGCGACCAAAGTCTGAACAAAGTGATTGGTACCTCGTTGTCTGATGCACC
Oa2151 / 1 / contig16366
4997-5064(+) / EU094075:
CTGCTATGATGACTTTCTTAGGACACCTTTGGATTAACTGTGAAAATACTAAAACATTCTGAGCAGC / C/D box 58A / NR_002571
chr.18 / CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAATAAATTCTGAGCAGC
Oa2156 / 1 / ultra270
437318-437412(+) / EU094076:
ctCACCGTGATGATGGTTCCAAACCATTCGCAGTTTCCACCAGAAAAGTCTTTTTCCTTAATGTTGGCTAAACCTTCCTTGGATGTCTGAGTGag / C/D box 14A / NR_000022
chr.11 / TCACTATGATGATTGGTTGCCAGACATTCGCAGTTTCCACCAGAAATGTTTTTCCTTATGTTGGCCAGTTCTTCCTTGGATGTCTGAGTGAG
Oa2171 / 1 / contig21556
1117-1191(-) / EU094077:
aaagtgatgagattccacttcattggtccgtGTTTCTGAAACTCATGATATGGTGGAAGTTCTGATTTGGCAGAA / C/D box 18B / NR_002442
chr.15 / TCAAAATGATGAGATTCCACTTAATTGGTCCGTGTTTCTGAAACACATGATATTTGTGGAAATTCTGACT
Oa2339 / 3 / contig59
2139260-2139337(+) / EU094078:
gCTCGATGATGATTGTCTCGTGTGATTGTTCTTTTGCAAGAGCAGAATGAACATACCATTTCACCGGCTCTGAGAGCT / C/D box 102 / NR_002574
chr.13 / AGCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACATTTCACCGGCTCTGAAAGCT
Oa2367 / 8 / no 100% match in Platypus-BLAST / EU094079:
TCACGTGATGATACTGACCAGTTCTGCTACTGAAGGGACGGCGATGACACCCTTAGAATCTGAAGC / C/D box 38A / NR_001456
chr.1 / TTCTCGTGATGAAAACTCTGTCCAGTTCTGCTACTGAAGGGAGAGAGATGAGAGCCTTTTAGGCTGAGGAA
Oa2371 / 5 / ultra520
1651535-1651600(+) / EU094080:
TTGAATGATGACTAGATTGTCGGATACCCCTACACTCCAACGTATGAGTGAAAAAAAAGTCTGACA / C/D box 101 / NR_002434
chr.6 / GTTTGAATGATGACTTTAATTGTCGGATACCCCTTCACTCCTTTTATGAGTGAAACATAAGAGTCTGACAAAC
Oa2572 / 7 / chr.7
6590951-6591019(+) / EU094081:
gttggctatgaagataaaatcatgcagattgaagttcCGTGATTGTTTTATACGCTATTCTGAGCCAac / C/D box 54 / NR_002437
chr.8 / TGGCGATGAGGAGGTACCTATTGTGTTGAGTAACGGTGATAATTTTATACGCTATTCTGAGCCGATT
Oa2691 / 1 / ultra497
338426-338492(-) / EU094082:
cgtgcagatgatggaaaagtttttttgCTGACAAAAGTGATGTTAACAAAGGAACCGCTGATGCacg / C/D box 42A / NR_000014
chr.17 / GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAACCACTGAAGTG
Oa2856 / 1 / contig14108
21138-21211(+) / EU094083:
agggccgatgatgctgttcaattacctacattgtttgattttcCTGTGAGAAAACAGCTATATCTGAGGCccct / C/D box 34 / NR_000019
chr.19 / CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCAGCACTGGCTGAGACGC
Oa2949 / 1 / contig11099
20223-20294(+) / EU094084:
TGCAGATGATGATCCATATATTTGCTATCTGAATAGCAGTGATTACAGCCTTACCACCAAGATCGCTGATGC / /
bOaU24 / contig11099
18675(+) / EU094085:
gtgcagatgatgcaaaagaacatccgctgtcagaatggtcgtggtgacacgtcgctaaccaccaagatcgctgatgcac / C/D box 24 / NR_002447
chr.9 / TGCAGATGATGTAAAAGAATATTTGCTATCTGAGAGATGGTGATGACATTTTAAACCACCAAGATCGCTGATGCA
bOaCD32 / contig14108
20541-20620(+) / EU094086:
ggccagtgatgaggaacattcaccatctttcgttcgagtctcgcgactgtgagaccaactccatgcaccgctctgaggcc / C/D box 32A / NR_000021
chr.19 / AGGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGAGTCTCACGGCCATGAGATCAACCCCATGCACCGCTCTGAGACCT
bOaCD36C / contig11099
21406-21479(+) / EU094087:
ccttttgccaatgatgcggagaatttcttcacctgaacgattatgtggccgctttacgatcctgagacaaaagg / C/D box 36C / NR_000016
chr.9 / TTGCCAATGATGGTTAAGAATTTCTTCACCTGAATAAACCATGTGGTCAGCATTGCATCTGAGGCAAA
bOaCD58B / contig16366
4488-4553(+) / EU094089:
gctgcgatgatgaccttcttaggacacctttggattaactgtgaaaacaaggaaattctgagcagc / C/D box 58B / NR_002572
chr.18 / atggCTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAACTACTCTCTGAGCAGCtgtt
bOaU58 / contig16366
6888-6957(+) / EU094090:
tggttgctgtgatgacaatcttaggacacctttggattaaccgtgaaaagaaatatattctgagcaatca / U58 / NR_002573
chr.18 / TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAACTATTCTGAGCAAC
bOaU86 / contig4527
34837-34890(-) / EU094091:
ggccgatgaagggctgagccgagaccccctccccaactgtgctctgctcctgaa / U86 / NR_000026
chr.22 / GGGGTGATGAAGGCCTGCACCTGGTCCCCTCCCCAACTCTGCTCTGCTCCTGAA
bOa2156i / ultra270
436459-436556(+) / EU094092:
cgctcactgtgatgattgcttccataccattcgcagtttccaccagaaaggttttatctttgtgttggctaaaccttccttggatgtctgagtgagtg / /
Table S 2: Full-length C/D box snoRNAs in introns of ribosomal protein genes (group II: conserved in mammals)
Ornithorhynchus anatinus / Homo sapiensname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1774 / 5 / contig819
313833-313915(-) / EU094093:
cgtgggaACGGGTGATGACCACATGTTTCGGTCCCAGATGACGCCGTGTGATTTCACCGTTGCGATTTTCTGACGTTCccacg / U73A / Z83334
chr.4 / GAATAAGTGATGAAAAAAGTTTCGGTCCCAGATGATGGCCAGTGATAACAACATTTTTCTGATGTT
bOaU73B / contig819
314989-315063(-) / EU094197:
gggatacgtgatgacaagatgtttcggtcccagatgacgtcccgtgatttgactgttgcatttttctgacgtccc / U73B / Z83334
chr.4 / GAATGAATGATGACAAAATGTTTCAGTCCCAAATGATACATACTGATTATACCATTATATTTATCCTGACATTC
Table S 3: Full-length C/D box snoRNAs in introns of ribosomal protein genes (group III: specific for platypus)
Ornithorhynchus anatinusname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence
Oa1735b / 38 / contig278044
590-666 (-) / EU094094:
TGTACATGATGATAGCTTGGCTCCCTCTACTGAAAATGGTGAGGATAATAGCCATGTCACCCAATATTATCTGAACg
Oa1781 / 2 / contig211336
5-80(-) / EU094095:
gggatgtgtgatgacaaaatgtttCGGTCCCAAATGACATTCCATGATTTGACTGTTGCATTTTTCCTGACATccc
Table S 4: Full-length C/D box snoRNAs in introns of snoRNa host genes (group I: conserved in vertebrates)
Ornithorhynchus anatinus / Homo sapiensName / Number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / Ortholog in human / NCBI
Acc-No / Sequence
Oa1692 / 87 / ultra42
84867-84937(+) / EU094096:
ATCAGTGATGAGCATATCCCGAACCTGACTTCTTGATGAAAACACTATTATACGGATCTGGCATCTGAGAA / U50` / AB017710
1701-1773
chr.6 / TAATCAATGATGAAACCTATCCCGAAGCTGATAAcctgaagaaaaataagtacggattcggcttctgagatta
Oa1702 / 3 / ultra341
3115312-3115372(-) / EU094097:
CTGTATGATGATGCTGCAAAGACTGATTCAATCATGATGGTCTTATTAGCTCTACCTGATC / U44 / AF141346
1961-2021
chr.1 / CCTGGATGATGATAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTCTAACTGACT
Oa1723 / 1 / contig18615
11213-11278(-)
1 mm:
g is missing in cDNA seq. / EU094098:
TGCAGGGATGATGTAAAAATGAACTCTCTCTTTCTGATGGTTCAGTG(g)GAGAAATTTTACTAATTTCTGAGTAAC / U26 / U40580
1013-1090
chr.11 / tgCTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGGAGAAAACAGAAAATTCTGAGTAGCa
Oa1724 / 10 / ultra341
3116474-3116560 / EU094099:
gagtgcCGCAATGATGACTCTTTATTTGCTACTCTTGACCATGAGAATGACGAGAGCTATTACCACCATTTTGATACTGAGGCACtt / U76 / AF141346
1274-1359
chr.1 / gTGCCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGGATCTTAACCACCATTATCTTAACTGAGGCac
Oa1764 / 110 / ultra341
3112925-3112993(-) / EU094100:
CACAAATGATGACCACATCCTTAATTGAACTCTCTCACTGATATTTGATGAGAATCTAAGGTCTGATGC / U81 / AF141346
3759-3839
chr.1 / tgcagaaTACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACttgatgacaataaaatatctgatattctgca
Oa1803 / 102 / ultra42
85176-85264(+) / EU094101:
cttggtctTGTCTGTGATGATTCTATCCCGAACCTGAACTCCTGTTGAAAAAAAACACAAACGGATCTGGCTTCTGAGATaagaccaag / U50A / AB017710
1992-2066
chr.6 / TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAACTTTTACGGATCTGGCTTCTGAGAT
Oa1805 / 5 / ultra341
3113140-3113217(-) / EU094102:
AACCAATGATGATAAGATTCTGCCAAATGAAAGAGACTGATTTCACCTATAAAACGTTCCTTTTTTTATTCTGAGGTT / U47 / AF141346
3549-3613
chr.1 / AACCAATGATGTAATGATTCTGCCAAATGAAATATAATGATATCACTGTAAAACCGTTCCATTTTGATTCTGAGGTT
Oa1836 / 5 / ultra341
3117487-3117553(-) / EU094103:
ACCTGTGATGAAAACAAGTGTTGGTAGGGACATCTGAGAAAGCTGATGAATGCCAACACAGCTGAGT / U74 / AF141346
245-316
chr.1 / CTGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGACAGTAATGATGAATGCCAACCGCTCTGATGGTGG
Oa1843 / 4 / ultra341
3116198-3116266(-) / EU094104:
gaTACAGTGATGCTTGCATAGTTCAGCAGATGGAACAGTGACGAAATATACCACATAATCTGATGtatc / U77 / AF141346
1617-1688
chr.1 / CAGATACTATGATGGTTGCATAGTTCAGCAGATTTAATCATGAAGAGATGTACTATCTGTCtgatgtatctg
Oa1892 / 9 / chr.7
1217665-1217744(+) / EU094105:
ggcATTAATGATGATTTTTCTTTTTGCCGTTTACCCGGCTGAGTGCTTCTTTGAAGTGGACTTAAATCTGAGAtgccat / /
Oa1907 / 11 / contig18615
8363-8488(-) / EU094106:
TCCTAATGAAGAAAACCAGCACCTGTCTTACTCCTTGTCCTGGCCCCAGAGCCTGCAAAGGCGAACCCACTGGGGTCGGCTGGGGGAGAAGATGATAATCTGTTCCAGCGGGAACAGTCTGAGGGAC / U22 / U40580
3346-3470
chr.11 / TCCCAATGAAGAAACTTTCACATGTCTTACTCTCTGTCCTAGTCCCAGAGCCTGTAAAGGTGAACCACTGGGACTGGCTGGGGGAGAAGAGGAAGATTTGTTCCAGAAGGAACTGTCTGAGGGAT
Oa1925 / 2 / contig18615
10082-10215(-) / EU094107:
gggtACTAATGAGGAAATCCACACCTGTCTTAGCTCCTGTCCAGTTCCAGAGCCTGCAAAGGCGAACCCACTGGAATTGGCAGGGGAGAGAGGAAGATAAAATGTTCCTGAGTGAACAGTCTGATGtgccc / pseudo-gene U22 / U40580
2098-2222
chr.11 / CTCCAATGAGGAGGAAACATCCTGCCTTACCCCTGTTTTAGCCTGGGAACCAGTAATTGTGAACTCACCAGGGTTAACATGAAGAGGGCATGAGAGCTTATTCCATAAGGAATTGTCTGAGACAT
Oa2342 / 1 / contig18615
10369-10449(-) / EU094108:
attgccagatgatttcaatccttattttctgatgctccgtgaggtacagatatcatagCATGTTAGAGTTCTGATGGCAat / U28 / U40580
1684-1760
chr.11 / ctGTCAGATGATTTGAATTGATAAGCTGATGTTCTGTGAGGTACAAAAGTTAATAGCATGTTAGAGTTCTGATGGCA
Oa2376 / 10 / contig18615
9844-9917(-) / EU094109:
gcagTTTCTATGATGATTCTAACTAGCTCACTTTGATAACTAAAATGAAAACCCTTGAACATCTGAGAAactgc / U29 / U40580
2412-2481
chr.11 / cagTTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGAACACCTGAGAAACtg
Oa2527 / 3 / ultra341
3113986-3114069(-) / EU094110:
ctgtgcTGTTCATGATGATTTGTTAAAATCAATGGGAATCTCTCTGAAGAGAATGAAGCCAGTTTTTATACTGAAACagtacag / U79 / AF141346 2555-2635
chr.1 / CTGTTAGTGATGATTTTAAAATTAAAGCAGATGGGAATCCCTCTGAGAAAGAAAATGGAGATTAATCTTAAACTGAAACAG
Oa2528 / 3 / ultra341
3114499-3114570(-) / EU094111:
AGCCAGTGATGACTTTAAATGATAAGAGTAGTGGACAGAAGAGATTTCTGAATACTTGAAACGCTGAGGCTT / U78 / AF141346
2302-2363
chr.1 / TGTAATGATGTTGATCAAATGTCTGACCTGAAATGAGCATGTAGACAAAGGTAcactgaaga
Oa2584 / 4 / contig18615
8683-8742(-) / EU094112:
TTCTGTGATGAATCTATACCGCCCCAGTCTGATCATTGTGACTGAAAGGTAATTCTGAGC / U31 / U40580
2989-3056
chr.11 / CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAAAGGTATTTTCTGAGCTGT
Oa2586 / 1 / chr.7
1218506-1218578(+) / EU094113:
CTTAATGATGATTAAAAAAATATTCGCCGTTTACCCAACTGAAAGTTTTTGATGTGCACTTTTTAATCTGAAA / C/D box 87 / NR_002598
chr.8 / ACAATGATGACTTAAATTACTTTTTGCCGTTTACCCAGCTGAGGTTGTCTTTGAAGAAATAATTTTAAGACTGAGA
Oa2684 / 2 / contig18615
11943-12012(-) / EU094114:
TCTCGGTGATGAAAAATTTTCACAGACCTGTACTGACATCTGTGAGGAAAAAATACTTTTCTGAAGAGAC / U25 / U40580
750-816
chr.11 / TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGATAAATAACTCTGAGGAGA
Oa2985 / ultra341
3113758-3113832(-) / EU094115:
TACAATGATGATAACATAGTTCAGCAGATGAAAAGTGATGAACTCTCATTTTTGTCTTTCGCTCCTATCTGATGT / U80 / AF141346
3078-3155
chr.1 / gatACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTAAGTCTTTCGCTCCTATCTGATGtatc
bOaU27 / contig18615
10644-10704(-) / EU094116:
tgatgaaggtaaaaaagacaagcatatggctgatggcatagtgatgtcactttacttctga / U27 / U40580
1298-1369
chr.11 / ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGATGTCATCTTACTACTGAGAAGT
bOaU30 / contig18615
9591-9655(-) / EU094117:
gatgtgatgaatttcttggaatctcgttcggctgaactctgctgttgagacttggaaatctgatt / U30 / U40580
2651-2720
chr.11 / GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGAGACTCTGAAATCTGATTTTC
bOaU75 / ultra341
3116775-311684(-) / EU094118:
aagcctatgatggttttaagtataagagtagtggacagatgtgatttctgacaactgactactgaggctt / U75 / AF141346
1052-1111
chr.1 / AGCCTGTGATGCTTTAAGAGTAGTGGACAGAAGGGATTTCTGAAATTCTATTCTGAGGCT
Table S 5: Full-length C/D box snoRNAs in introns of genes other than ribosomal protein genes or snoRNA host genes (group I: conserved in vertebrates)
Ornithorhynchus anatinus / Homo sapiensname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1712 / 4 / contig2379
87568-87645(+) / EU094119:
CTCTCCTGATGACTCTTCTCTTCCTGACTGGCTCCAGTGATGAGTAAACCTGAGCCTCTAAGAAGTTGACTGAAGAGG / C/D box 125 / AM413037
chr.22 / ACCCCTGGCAGCCCCTCCTGATGATTCTTCTTCCTGAGCACGCTCATGATGAGCAAACTGAGCCTCTAAGAAGTTGACTGAAGGGGCTGCTTCCCC
Oa1716 / 147 / contig917
32925-33000(-) / EU094120:
gggggAGTTATGATGTATTTAAGTCCTATTCCATTGCTGAAATACAGTGTGGAACACGATGAACTGAACTCctctt / C/D box 98 / NR_003076
chr.10 / GAGTTATGATGTGTGTAAATCCTATTCCATTGCTGAAATGCAGTGTGGAACACAATGAACTGAACTC
Oa1733 / 34 / contig30838
4981-5046(+) / EU094121:
CACAAATGATGAGCCTAAAGGGACTTAATACTGAAACCTGATGTAACTAAATAATATATGCTGATC / U82 / AJ011568
chr.2 / ACAGCACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTTACATTGTAGTGTGCTGATGTGCTGT
Oa1745 / 23 / contig21023
5895-5969(+) / EU094122:
tcCTTCGCTGATGAGTGACTCTTTGACTTTCGTTCTTCTGAGTCTGCTGAAGCCATTTGTCTATTCTGAGAAGGA / C/D box 59B / NR_003046
chr.12 / TATTCCTCACTGATGAGTACGTTCTGACTTTCGTTCTTCTGAGTTTGCTGAAGCCAGATGCAATTTCTGAGAAGG
Oa1760 / 9 / contig32194
2732-2817(-) / EU094123:
ggcAACCGTGATGACAGGTTCAGTCTGTATGTGTAATGGGTTTCATTACTACATGAAGACACCAGTCCCTCTAAGCTGATGttgcc / C/D box 118 / AM413025
chr.14 / TTTCACTGTGGCAACTGTGATGAAAGATTTGGTCTGTATGTAATAGATTTTATTACTAAATGAGGACAACAGTCCCTCTAAACTGATGTTGCCATTTAAAA
Oa1770 / 3 / chr. 3
31449109-31449189(+) / EU094124:
tccagaaGACAGTGATGTGGTATTTTCCAAGCACATATCTGATTGGCATGTGTGGAAATCATTATTTCTGAGTTttctgga / /
Oa1784 / 2 / contig5329
24960-25033(-) / EU094125:
gtgTGCCGTGATGGATTTAACGATCGACACATCATTCCGAAACGAGCGGTGTGGACAGCTAATGACTGAGCacag / C/D box 63 / NR_002913
chr.5 / GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAAAACTAATGACTGAGCACA
Oa1786 / 6 / ultra55
414926-415005(+) / EU094126:
tgcCTGAAATGATGACTATTTCATGTCTCTTCTGAGACTCCTGAAGCTCTGGAGATAGCACTTACCTCTCTGATCAGGCa / /
Oa1791 / 21 / contig14117
4293-4378(-) / EU094128:
ccccgGCCACATGATGCAATAGTCTGAGACGGGAGCGCCACGCGCCAGGACGACCTTCATCTATCACCCTTGACTGATGGCTgggg / /
Oa1811 / 16 / chr.3
31470346-31470417(+) / EU094129:
gaaAGACAATGATGCCTGATTTCCAAGCACATATCTGACCTAATTTGAGGAGAACTCAAGACTGAGTCTTTC / C/D box 121B / AM413032
chr.9 / CTAACTGGAAAAGACAATGATGTTTTATTTCCAAGCACATATCTGAGTTGTATGTGTGGACAGCACTGAGACTGAGTCTTTCCACAGCTAGGA
Oa1814 / 14 / contig17567
10366-10438(-) / EU094130:
CCGCCGTGATGGAACGATTTTTCGTCAACAGCAGTTCACCCCCGTGAGTGTTGATAAGCCTGTGTCTGAGCGA / U56 / X96655
chr.20 / CCACAATGATGGCAATATTTTTCGTCAACAGCAGTTCACCTAGTGAGTGTTGAGACTCTGGGTCTGAGTGA
Oa1815 / 22 / chr. 6
7382941-7383015(+) / EU094131:
GGTCCATGATGAATTTCACGGCGTTGATCGTCCGAAGTGATGATGCATCCTTGTCCTCTAAGAAGTTCTGAGATC / C/D box 61 / NR_002903
chr.X / GCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTTTTGTCCTCTAAGAAGTTCTGAGCTT
Oa1833 / 3 / ultra70
203093-203177(+) / EU094132:
GTCTAATGATGAGGACTCATAGGGCAGATTCTGAGGTGAGCACTGAACTCATCACTGATAATTGTTTGATGCGATCTGAAGACAC / C/D box 90 / NR_003071
chr.9 / AAGTTTTCTAAGTGTCTAATGATGAATTTCATAGGGCAGATTCTGAGGTGAAAATTTAATTCATCACTGATACTCCTACTGTGGAATCTGAAGACACTTGAAAACGT
Oa1927 / 4 / contig7489
18153-18246(-) / EU094133:
CTGCTGTGATGCAATGTTCCAAACCATTCGTAGTTTCCACCTGAAGTCCCGAGACTCATGTTGGCTCAAGTTCCTTCCTTGGATGTCTGAGCGG / /
Oa1928 / 16 / ultra170
93714-93785(-) / EU094134:
GCTACATGATGAATCAAATCAAATCACCATCTTTCGGCTGAGATCGTGATGGATCTGCTTTATTCTGACCAA / C/D box 51 / NR_002589
chr.2 / GTTGCATGATGAATAAAATCAAATCACCATCTTTCGGCTGAGTTCGTGATGGATTTGCTTTTTTCTGATT
Oa1943 / 8 / ultra55
417206-417281(+) / EU094135:
TGAAATGATGAGTTCACTTCATGTCTCTTCTCTGAAATCTTCCCTGGAGACACAATTAACCTCGCTGATCTGATCA / C/D box 111 / NR_003079
chr.16 / CAGCCTGAAATGATGACTCTTTAAAAAATTTCATGTCTCTTCTCTGACATTTTTCTCTGGACACAGTTTTTGCCTTATGAATCTGATCAGGCTG
Oa1976 / 5 / contig12565
11479-11550(-) / EU094189:
tCTGCTGGGATGAACTGCATGTTCAGTCCGTGTTTCACCTGATTCCTGATTACACACATGCACTGAGCAGA / /
Oa1989 / 3 / contig7489
20490- 20583(-) / EU094136:
gaTCGCTGTGATGAGTATTTCCAAAACCATTCGTAGTTTCCACCAGAAGTTGACTAATGATGGCAAAGTACCTTCCTTGGATGTCTGAGCGAtc / /
Oa2032 / 3 / chr.3
31452954-31453033(+) / EU094138:
ccagaaGACAATGATGTGTGATTTCCAAGCACATATCCGATTTCTCCATGTGGAAATCCAACCTCGACTGAGTtttctgg / C/D box 121A / AM13031
chr.9 / CTTAGTCCAGAAAACAATGATGTGGTAATTTCCAAGCACATATCTGATGATTCCATGTGGAATTTAACTACCTGAGTTTCCTGGACAAAGA
Oa2118a / 1 / contig10764835-913(+) / EU094139:
GTCAATGATGCATTCATGTTGAAACTGAATCCAAGTGATTTAACTGAACAATTCGTCACTACCACTGAGAC / new C/D / NT_005403
53352247-53352333
chr.2 / ATTCAATGTTGTCAATGATGCATTCTTATTGGAACTGAATTTAAGTGATCTGACTCATTCGTCACTACCACTGAGACAACATTGAGT
Oa2118b / 2 / no 100% match in Platypus-BLAST / EU094140:
GTCAATGATGCATTCATGTTGAAACTGAATCCAAGTGATTTAACTGGACAATTCGTCACTACCACTGAGAC / C/D box 70 / NR_003058
chr.2 / TTCGTTGTTGTCAATGATGTATTCTTCTTGGAACTGAATCTAAGTGATCTGACTCAATATTCGTCACTACCACTGAGACAACGATGAA
Oa2134 / 7 / contig12565
11070-11142(-) / EU094174:
CCTGCCTTGATGAAATGCATGTTCAGTCCGTGTTTCAGCTGACCACCCTGATTATACACATGCGCTGAGCAGG / C/D Box 126 / AM413038
chr.14 / CTCAGTCATTTACAGTTTGCCATGATGAAATGCATGTTAAGTCCGTGTTTCAGCTGATCAGCCTGATTAAACACATGCTCTGAGCAGACTAAAGCTAG
Oa2337 / 14 / no 100% match in Platpus-BLAST / EU094141:
CTCCGCTGATGACCTTTTCCCAATGACTTTCGTTCTTCTGAGCTTGCTGAAGCCGCTCTTAGGGTCTGAGGAGAAC / C/D Box 59A / NR_002737
chr.12 / CCTTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGAAGCCACATTTAGGTACTGAGAAGG
Oa2364 / 14 / contig14104
10029-1009(-) / EU094142:
CCACATGATGATTAAACATTTCTTCACTTTGACCTGATGTCTACTGAAGAAATCCAGTATCTGACA / /
Oa2369 / 2 / contig1718
52673-52738(-) / EU094143:
gAGAAGTGATGAGGAAACAAATAGTCTTACTGTATAAGTGAAGACATCAGATCCAACTCTGATCtt / new C/D / NT_022517
52665432-
52665502
chr.3 / GCAAGAAATGAAGAAACTAAAATTGGTCTTAGTATTGAAGTGAAGACACTGAGATCCAACTCTGATCTTGC
Oa2372 / 1 / ultra696
25205-25274(+) / EU094144.
GAGCCGTGATGAACTCTTAACATGCCATCTGATTGCCCTTGCTGAAACCCAGAGGCTGTTTCTGAGCATC / C/D box 95 / NR_002591
chr.5 / GCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGC
Oa2470 / 2 / contig1718
52122-52185(-) / EU094145.
aAGCCTATGATGAAAGTTGGAACTGACATACCATGCTGAGTTTGTTCAATCCAACCCTGAGCtt / C/D box 69 / NR_003057
chr.3 / AATGTGAAGCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCCAACCCTGAGCTTCATGTT
Oa2681 / 2 / contig17567
10060-10134(-) / EU094146:
gggTGGAGATGATGAGCCACTTGGTCCTGAACCTAGCGTGAGGGCAAAAGCTGATTTAATGAGCCTGATCCACcc / C/D box 57 / NR_002738
chr.20 / TGGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAATGGAGGCAAAAAAACTGATTTAATGAGCCTGATCC
Oa2717 / 1 / contig5910
19700-19789(-) / EU094147:
ttcaaggtcagtgatgtgttggcatgTATTATCTGAATCTAAAGTTGATGCTTACCATTCCTATAGCTCTAGGATTACTGAGACcttgaa / /
Oa2916 / 1 / ultra696
27900-27971(+) / EU094148:
gggTCCGGTGATGAGATGACGCATGTTGGCCGACCCCTGCTGACGACACGTCCTGCAATTCTGAAGggaccc / /
Oa2977 / 1 / contig46167
6327-6404(+) / EU094161:
gGGAAATGATGATTTCGTGTTTTACCATCTTTCGGGACTGATGTGGAAATGATGATAAACATGTTTCTCTGATTgccc / /
Oa2993 / 1 / contig1718
53553-53619(-) / EU094149:
TGAACTGTGATGAAACTGATCGCTCTGGATACAAGAGTGAAGAATACAAGATCCAACTCTGATTTCA / C/D box 117 / AM413023
chr.3 / TTCAGATTTTGGTTGAAATATGATGAGTGTACAAAATCTTGATTTAAGTGAATGAAAAATTACAAGATCCAACTCTGATTTCAGCCAGAGATC
bOaU14i1 / contig7489
19298-19387(-) / EU094150:
tcgctgtgatgaatgcttccaaaaccattcgtagtttccaccagaaggtgactgatgatggctaagttccttccttggatgtctgagcga / U14 / Y00371
2941-3032
chr.11 / ACTCGCTGTGATGAGTGATTGTTAAACATTCGTAGTTTCCACCAAAAGCTTGGCTAATGATGGCAACACCTTCCTTGGATGTCTGAGCGAGT
bOaU14i2 / contig7489
18803-18904(-) / EU094151:
cttgtcgctgtgatgaaaatgaccccaaaccattcgtagtttccaccagaagttgagacatgtgttggcccaagttccttccttggatgtctgagcgacgag / U14 / Y00371
3370-3456
chr.11 / TCGCTATGATGATGGATTCCAAAACCATTCGTAGTTTCCACCAGAAAGTCTTATGTTGGCCAGTTCCTTCCTTGGATGTTTGAGCGA
bOaU14i3 / contig7489
17502-17597(-) / EU094152:
tggttgctatgatagattccaaaaccatttgtagtttccaccagaagctgacacatgttggcttaaaagttccttccttggatgtctgagcgacta / U14 / Y00371
4200-4290
chr.11 / GGGTCACAATGATGAATGGTCCAAAACATTCGCGGTTTCCACCAGAATTCAAGGTGTTGGCAACTACCTTCCTTGGATGTCTGAGTGACCC
bOaU84 / contig14117
5789-5863(-) / EU094153:
gccacatgatgttttttatcgagacagagcgccctgtgcggagatgacctcgatctatcacccttgactgatggt / U84 / NR_003065
Chr.6 / cggcgGCCATATGATGTTTTCTTTTCGAAAGGTGAGCGCTTTGCGCAGTGATGACCCTCATCTATCACCCTTGACTGATGGCTGctg
bOaU95i1 / ultra696
28253-28316(+) / EU094154:
gcgctgatgatcactaacatgccatctgactgcccttgctgaaatccagaggctgtttctgagc / /
bOACD
45A / contig5910
23420-23513(-) / EU094155:
tttcagggtcaatgatgttttggcatgtattacctgaatctaaagttgatgctaattacctcttcagctctagaattactctgagaccctgaaa / C/D box 45A / NR_002749
chr.1 / GGTCAATGATGTGTTGGCATGTATTATCTGAATCTATTGCTGATGTGTAATAACACTTTAGCTCTAGAATTACTCTGAGACCTT
bOACD
45B / contig5910
21624-21712(-) / EU094156:
ccaaggtcaatgatggttggcatgtattatctgaatctaaagttgatgtttatcaccacattagctctagaattactctgagaccttgg / C/D box 45B / NR_002748
chr.1 / CAAGGTCAATGATGTAATGGCATGTATTAGCTGAATCTAAAGTTGATGTGAGTTCTAAAATTACACTGAGACCTTG
bOACD
45C / contig5910
23935-24011(-) / EU094157:
gtcaatgatgagttggcatgtattctgaatctaaagttgatgtttatcaccatttagctctagaattactctgaggc / C/D box 45C / NR_003042
chr.1 / GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAGTTGATTATTACTACTTTAGCTCTAGAATTACTCTGAGACCT
bOaCD20 / contig30838
1799-1875(-) / EU094158:
tggatatgatgactgattatctgagacaaactgatgaaaccaataaaaattcctctagatagtcaagttctgatcca / C/D box 20 / NR_002908
chr.2 / CTGGATATGATGACTGATTACCTGAGAAATAATTGATGAAATCTCAAGAAAATTCCTCTAGATAGTCAAGTTCTGATCCAG
bOaCD
66i / contig194710
216-299(-) / EU094159:
cctcttcgctgatgactccctgttcgtgccgtcgtgtctgggccactgagacctggtgatggagatgaggatctgaggaagggg / /
bOaCD
111B / Ultra55
413945- 414035(+) / EU094198:
gggaaggcctggaatgatgatccagattcatgtctcttctctgagatttcctctggagaaaatcctgttttttatctgatcgggccttccc / C/D box 111B / AM413027
chr.16 / CTTTCATCAGCCTGAAGTGATGATTCACATTCATGTCTCTTCTCTGATAAATTCTTGAAGAAAATTTTTGTGTGTCTGATCAGGCCTCTAGAGG
bOaCD
117/69i1 / contig1718
54508-54581(-) / EU094160:
gggcaagagatgatgatgatacacatagtcttattgtgacggtgaagacatcagatccaactctgattttgccc / new C/D / NT_022517
52662944-
52663011
chr.3 / GTGAGAAATGATGAGGGTCAACATTCTTCATACCAAAGTGAAGACATGAGATCCAACTCTGAGCTCAC
bOaCD19 / Contig1718
54148-54213(-) / aggaatgaggaaaatatctgatcagtctgatataccactgaagactgtaagatccaactctgacct / C/D box 19 / NR_003047
chr.3 / ACATGGGTGAGGTACGAGGAAACAGTCTGATAGTCACTGAAGACTGATTAGATCCAACTCTGATCTCAGCAAAGCC
Table S 6: Full-length C/D box snoRNAs in introns of genes other than ribosomal protein genes or snoRNA host genes (group II: conserved in mammals)
Ornithorhynchus anatinus / Homo sapiensname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1787 / 9 / contig34618
1583-1660(+) / EU094127:
CTCCGCTGACGAATGCTTGTTTGAGCCATCGTGTCTGGGCCACTGACACCCCGTGATGGAACTGAGGATCTGAGGAGA
Oa2015 / 2 / contig23780
5744-5822(+) / EU094137:
TGGCAGGGATGACACACTTGCCCTCGCATAGATTCTGCTGATGAGAACATCTCTCCGTACTCGACTGTTCTGAGCCAGC / C/D box 85 / NR_003066
chr.1 / GACTGGCAAGGATGATACACACTTGCCCTCACTTAGACTATAGTTCACTGATGAGAGCATTGTTCTGAGCCAGTC
Table S 7: Full-length C/D box snoRNAs in introns of genes other than ribosomal protein genes or snoRNA host genes (group III: specific for platypus)
Ornithorhynchus anatinusname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence
bOaU70i / contig10764
2794-2872(+) / EU094162:
tgttgtcaatgatgcattcttgttggaactgaatcaaaatgatttaattcaacaattcgtcactaccactgagacaaca
bOaU95i2 / ultra696
29002-29074(+) / EU094163:
cagagcgatgaagagttctaacatgccatctgagctgcccctgctgagaccaagaggctgtttctgagctctg
Table S 8: Full-length C/D box snoRNAs in intergenic regions (group I: conserved in vertebrates)
Ornithorhynchus anatinus / Homo sapiensname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1758 / 5 / contig24898
6933-7002(-) / EU094164:
gctgGTTAAGGATGACACTCAATGGTGGACATGTATGGATGACACTTGCGGATATGGGACTGAGACcagc / C/D box 99 / NR_003077
chr.1 / ACTGGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGGATATGGGACTGAGACCAGCTCCTAGG
Oa1765 / 11 / contig21402
4519-4589(+) / EU094165:
gAGCTAGTGATGAGTAAAAGTTATCCCTGTCTGAACAAAATCCTTTGTGGAATTTTGATTTCCTGAGGCTC / R38C / AJ543326
chr.17 / tgGcTGAGCTGAGGATGATTTAAAGTTATCCCTGTCTGAAATGGTATCTTTTGTGAGGAGGTCTGACTTGCTGAGGCTCAGCtg
Oa1797 / 23 / ultra288
3309558-3309626(-) / EU094166:
CTTGCTGTGATGACTTCCCTTGAAACGTGTTAGACTGCTGATGCGAATGATGCCACTTCCAATCTGAGc / U104 / AY349605
chr.17 / GGCCTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGTGATGCGAACTGGAGTCTGAGCCTGCC
Oa1850a / 4 / ultra516
1569155-1569245(+) / EU094167:
gctggtGTATGTGATGACGAAACTTTTTTCCCCATCAGATCGACCATGTTGATCTCAATTCCTCTTTGCCAGGTATTTTCTGATACaccag / C/ box 12B / AM413026
chr.20 / CTTCCTGCTGGCATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCTAACTTTTCTAAGCCAGTTTCTGTCTGATATGCCAGCTTGAGC
Oa1900 / 11 / ultra42
1071457-1071533(+) / EU094168:
CTCAGTGATGAGAACAAACCCCAAAACAGCTGGAATTACCGGCAGAGTCCCCGTGGTGACTCCGGTTTCTCTGAGTG / / / /
Oa2049 / 4 / ultra202
1019603-1019686(+) / EU094169:
ATGCTGTGATGACGTTTTCAACTGTGGTTTCTGTTCACCGACGGTTGAATGAGGACAGATAATTCCTTGGCTGTGTCTGAGCAT / C/D box 92 / NR_003074
chr.2 / TGGTGCTGTGATGATGCCTTAATATTGTGGTTTCGACTCACTGAGAGTAAAATGAGGACCTACAATTCCTTGGCTGTGTCTGAGCACCC
Oa2051 / 1 / no 100% match in Platypus-BLAST / EU094170:
CTCTGTGATGGCCGGCACTGGGCTCTGACCTCCTCTGAGGACACCGTGCGCCCTGGGACTTTGACACCCAGGGAATCTGAC / U88C
or
U88A
or
U88B / NR_003069
chr.19
NR_003067
chr.19
NR_003068
chr.19 / U88C:
CTGGGGCTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGCACCCCAGGACCTTTGACACCTGGGGGTCTGAGGGGCCCCAG
U88A:
CCGGGGCCTCCATGATGTCCAGCACTGGGCTCCGACTGCCACTGAGGACACGGTGCCCCCCGGGACCTTTGACACCCGGGGGTCTGAGGGGCCCTGG
U88B
TTGGGGACCCCGTGATGTCCAGCACTGGGCTCTGACTGCCCCTGAGGACACGGTGCACCCCGGGACCTTTGACATCCGGGGTTCTGAGGGGCCCCAC
Oa2054 / 1 / chr.4
20025608-20025691(-) / EU094171:
TCTCTATGATGTATTCCAATAGATCCTTCTGACCCTCCAGTGTGCATTCCAAAGAAGGGGAAATGAAGAGATGtGTGACTGAGAG / C/D box 62B
or
C/D box 62A
(in human both are 100% identical, 4734 bp between them / NR_003050
chr.9
or
NR_002914
chr.9 / TCTCAGTGATGTAATTCCAATAGATCCTTCTGACCCTCCACTGTGGACTCAATAGCAGGGAGATGAAGAGGACAGTGACTGAGAGA
Oa2111 / 1 / ultra42
1071101-1071181(+) / EU094172:
cCCAGATGATGAGAACCGTGGATAGGAAGTGCCGTCAGATGCGAGAACTGACGATGGATTTTCACGGGGTCTGACTggg / C/D box 49B / NR_003043
chr.17 / cgactgtcCTGATGATACTTGTAATAGGAAGTGCCGTCAGAAGCGATAACTGACGAcgtctaatgtctatctgaccgcagtcg
Oa2116 / 4 / ultra516
1569553-1569641(+) / EU094173:
ctggtGTATATGATGACTGCACTTTTTTCCCCATCAGATCGACCATGTTGATCTCACTACACTATGCCAGGTTCAATCTGATGcaccag / C/D box 12 / NR_003030
chr.20 / GCCTTTGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCTACACTATTGGCCAGTTTTGTCTGATGCATTGGC
Oa2340 / 1 / no 100% match in Platypus-BLAST / EU094175:
CCTTGTGATGTCTAGCACTGGGCTCCGATTCCTTCTGAGGACACTGTGCCCCCTGGGACTTTGACACCCAGGGAGTCTGAGGC / U88C
or
U88A
or
U88B / NR_003069
chr.19
NR_003067
chr.19
NR_003068
chr.19 / U88C:
CTGGGGCTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGCACCCCAGGACCTTTGACACCTGGGGGTCTGAGGGGCCCCAG
U88A:
CCGGGGCCTCCATGATGTCCAGCACTGGGCTCCGACTGCCACTGAGGACACGGTGCCCCCCGGGACCTTTGACACCCGGGGGTCTGAGGGGCCCTGG
U88B
TTGGGGACCCCGTGATGTCCAGCACTGGGCTCTGACTGCCCCTGAGGACACGGTGCACCCCGGGACCTTTGACATCCGGGGTTCTGAGGGGCCCCAC
Oa2872 / 1 / ultra516
1568423-1568512(+) / EU094176:
ctggtGTAAATGATGACTAAACTTTTTTCCCCATCAGATCGACTGTGTTGATCTCATCGCTCtttggccattttcattctgatacaccag / U106 / AY349607
chr.20 / TGTAAATGATGACTTCACTTTTTTCCCCATCAGATCGACAATGCTGACGTCTTATATTTTGCCAGTTAGTTCTGATACA
Oa3060 / 10 / ultra42
1071852-1071938(+) / EU094177:
ggCTGCAATGATGATAACATGGAATATAGGAAGTGCCGTCAGATGCGATAACTGACGATAATCTCTGTACCTAATCTGACCGCAGCt / C/D box 49A / NR_002744
chr.17 / gcttgacTGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGACGAAGACTACTCCTGTCTGATTgcagtcaagt
bOaU88i / contig219929
544-599(-) / EU094178:
nnttccttctgaggacgctgtgccccctggaactttgacacccagggagtctgagggg / U88C
or
U88A
or
U88B / NR_003069
chr.19
NR_003067
chr.19
NR_003068
chr.19 / U88C:
CTGGGGCTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGCACCCCAGGACCTTTGACACCTGGGGGTCTGAGGGGCCCCAG
U88A:
CCGGGGCCTCCATGATGTCCAGCACTGGGCTCCGACTGCCACTGAGGACACGGTGCCCCCCGGGACCTTTGACACCCGGGGGTCTGAGGGGCCCTGG
U88B
TTGGGGACCCCGTGATGTCCAGCACTGGGCTCTGACTGCCCCTGAGGACACGGTGCACCCCGGGACCTTTGACATCCGGGGTTCTGAGGGGCCCCAC
bOaCD65 / ultra42
1072380-1072458(+) / EU094180:
tattggatgatgaaagttaccaaaatagctggaattaccggcagatggaatggtggtgagccatggttttctgaagatg / C/D box 65 / NR_003054
chr.17 / AAGATATCAAATGATGAAATCACCCAAAATAGCTGGAATTACCGGCAGATTGTGTAGTGGTGAACCTATGGTTTTCTGAAGATATCTCTT
bOaR38B / contig21402
5297-5374(+) / EU094181:
gagttgatgatgctactgagttatccctgactgaagaggaatattcctccccttgtggagatcagacttgctgaactt / R38B / AJ543325
chr.17 / gggaGCTGAGTCCATGATGATTTCAAGTTATCCCTGTCTGAAGGCAAAGAAAGGCCTTTCTGTGTGGAATTTGAATATCTGAAACTCAGTttcc
bOaCD6 / ultra29
2671161-2671229(-) / atgttatgatgattggccaaatgttcaactgctctgaagagagtgaatgaaaacagcctttctgaacat / C/D box 6 / NR_003036
chr.11 / GATGTTATGATGATGGGCGAAATGTTCAACTGCTCTGAAGGGGCTGAATGAAAATGGCCTTTCTGAACATC
Table S 9: Full-length C/D box snoRNAs in in intergenic regions (group II: conserved in mammals)
Ornithorhynchus anatinus / Homo sapiensname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
bOaCD5 / ultra29
2674044-2674113(-) / attcaagtgatgacattattgttcaactgctaagtgatgtagatatgaacaaaaaggaattctgaggggt / C/D box 5 / NR_003033
chr.11 / GTTCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAACTAAAACTTAATTCTGACAGAGc
Table S 10: Full-length C/D box snoRNAs in in intergenic regions (group III: specific for platypus)
Ornithorhynchus anatinusname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence
bOa2049i1 / ultra202
1022832-1022915(+) / EU094195:
atgctgtgatgacattttcaatagtggtttctgttcactgacgggtgaatgaggacagataattccttggctgtgtctgagcat
bOa2049i2 / ultra202
1025700-1025783(+) / EU094196:
atgctatgatgatcacttcaactgtggtttcaactcaccgactgttgaatgaggacatgaaattccttggctgtgtctgagcat
Table S 11: Full-length C/D box snoRNAs, genomic locus not assignable (group III: specific for platypus)
Ornithorhynchus anatinusname / number of seq. clones / Sanger genomic location / NCBI Acc-No and sequence
Oa1747 / 4 / contig12555
1026-1099(-) / EU094182:
tATCCGATGATTAAACCACCTTCACTGCTGTTCACATTATGAGCGACTGATTATTTTATGTCTGAATGAGGATA
Oa1759 / 35 / no 100% match in Platypus-BLAST / EU094183:
GGAGACGTGATGTGTCAGTGAAATCTGTCAATCCTCTGAAACCTCCTGAACGACACACCCGTCACTGCTGATCTC
Oa1767 / 83 / contig10522
37932-38024(-) / EU094184:
GTGCGTGATGACAACTTTGGCCGTAGGAAAGCTTGGGGACCGGTTGGTCTTCACGAAGCTCCCTGTGACGGCACTGTCATAGTTACCCTGAAT
Oa1782 / 10 / ultra519
9351480-9351559(-) / EU094185:
gaGCCACATGATGAACCCTGAGACGTCACTAGCATACCGTGCAAAGATGACTCACATCTATCACCCAAGACTGATGGTTC
Oa1829 / 8 / contig29628
3943-4020(+) / EU094186:
cgggCACATGTGATGAATCCATTGTCCGCTGAGCCACGCGGTGACTTGACTAACAAAGTAAACGCTCTGATGTGCccg
Oa1850b / 4 / no 100% match in Platypus-BLAST / EU094187:
GTATGTGATGACGAAACTTTTTTCCCCATCAGATCGGCCATGTTGATCTCAATTCCTCTTTGCCAGGTATTTTCTGATAC
Oa1904 / 1 / contig45376
89-177(+) / EU094188:
gtggaaaatgatgacttggaacttttttccccaacagatcgacagtgttgatccaagaagcattttagCCAAAACCTGTCTGATTCcac
Oa2095 / 1 / chr.1
20654940-20655013(-) / EU094190:
tcgtctgtgaaatttcaagattcTCCGGGGACACCCTGATTCAAGAATTCAGGACCCCATCTAGTTCTGAGCAC
Oa2109 / 1 / no 100% match in platypus-BLAST / EU094191:
TGCCGTGATGCGTTTTTTCCAACACGTCATTCTGAAACCCAAAGTGTGGTGGCTTTTCGACTGAGCA
Oa2137 / 3 / ultra341
3114964-3115023(-) / EU094192:
TACAATGATGTCTTCAAATGTCTGACCTGAAAGTCTATGTAGACAAGCTTGCTTCTGAAA
Oa2712 / 1 / contig254794
669-726(+) / EU094193:
CACAATGATGACCAAATTTTGACGGAATCAACACATGTTGATTGTTATCCCTGGCTGA
Oa2939 / 1 / ultra337
2811205-2811281(-) / EU094194:
ctgaaggtattgcatgatgagacATTCCTTATGGAACCTTTCTGGGCAAGGTCCAACGCTACTGATAAtaccttcag
bOa
CD38A/Bi / ultra56
5367576-5367654(-) / EU094088:
agccttcacgtgatgataatattgtccagttctgctactgaagggacagcggtgacacccttagaatctgaagaaggct
bOaCD62i / contig139675
842-929(-) / EU094179:
gtctctatgatgtgttccaatagatccttctgaccctccactgtgcattccaaagaagggcaaatgaagagatgtgtgactgagagac
For tables S12-S19:
Column 1: Experimentally identified snoRNAs are indicated by Oa and a contig number. Computationally found snoRNAs are labeled bOa followed by a common name or the contig number of a corresponding paralog (label i).
Column 2: The number of sequence-clones presented in the cDNAs library.
Column 3: Sanger genomic location.
Column 4: Genbank accession-number and H/ACA-box snoRNA full-length sequences. Capital letters represent sequences from the cDNA library. Lower case letters represent genomic BLAST sequences. cDNAs are extended to presumed full-length.
Column 5: Human orthologs.
Column 6: Human GenBank accession numbers.
Column 7: Human ortholog H/ACA-box snoRNA.
----- denotes box H and ACA.
Table S 12:Full-length H/ACA-box snoRNAs in introns of ribosomal protein genes (group I: conserved in vertebrates)
Ornithorhynchus anatinus / Homo sapiensname / numb. of seq. clones / Sanger
genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1877
/ 8 / contig1129912723-12853(+) / EU093990:
tttTTGCAAGACATACCCAACACTGCTCTGAGTCTGAGAGATAGTGTGAATCAGTTCTTGCGACAGAATCGTCCTGCTGGAAAGCAGTGGCTCTGTTTTCTGACTTAGTGAGCTTTCCGGTAGGCACAGCT / /
Oa1953
/ 2 / contig59535361-5499(+) / EU093996:
aAACCCTTCCAAAAGCACTCATCTGGCCTCAACCCCGTGACCCGATGAGCCGTCGTGAAAGGGCAGAGCAAGAAACATCGCTTTTGGGTGAAGGAGCAGCAACTTGTGCTGCTTTACTTCAATCGGCGATGAGACAGGC / H/ACA box 21 / NR_002576
chr.17 / CCCCCTTTTAAAAGCACTCAATGGGCCTGTGGCTAATGACCTATTGAGCCGTCAAGAAAGGGGAGAGTGAAAACATCGCTTTTGGGTGAAGTGGCAACATGTGTTGTTTGCTTCAATCGGTGGTGTGACAAGG
Oa1973 / 4 / ultra117
1693908-1694062(-) / EU094008:
cCCGCACACTATTAAAGCTCAGAGCTGAGGCCGGTTCCGGGCCTATGAACTCCTGAGTGTCGGAAGTGTGCCACAGTAAAGCGGGCAGGATTTTCGCTAACACCAGGAGAGCTCGCCTCCGTGACTGGGGTTTGATAGTACTCGCTGCCACACGC / H/ACA box 6 / NR_002325
chr.3 / TGCACACTATTAAAGCTCAGGGTGGAGGCCAGTCTTGGCTCATGAACTTCTGAGTGTCGGAAGTGTGCTATATCAATGGCAGGATTTTCGCTAACACCAGTAGAGCTTGCCTCTATGACTGGAGTTTGGTAGTACTCGCTGCCACATAG
Oa2055 / 2 / contig59
2139607-2139737(+) / EU093991:
cCCCCCCTTTCACTTTGCCAGTTAGACATTTCTCTTGTCTTTATTGGTCATTCAAATGGGGTAAATTAATTTCCTTTTAAAACTCAAGCAAACAAATTTGTTTGTCTTGTATCCTATCAGGGGAGACATTT / H/ACA box 27 / NR_002575
chr.13 / TACCCCCTTTTCACTTTGCCAGTTGGACTTATGTCTTTATTGGTCATTCAAGTGGGGCAAAGGAAATATCCTTTTAAAACTCAGGCAAACTGGGTGTTTGTCTGTATCCTGTCAGAGGAAACAAAT
Oa2102 / 3 / ultra753
21316-21449(+) / EU093992:
tCTGCGAATATTCTCGCTGGCCTGATATGCCCGTGATCAGGTCAGGCTAGACATTCGCTATATTGAGACCCAGCATTGTGTCCCCGGGATTAGTCCTTGCCCTAATCCTGGTTTTGCAAATGCTGGTGACAAAT / H/ACA box 72 / NR_002581
chr.8 / CTGCGAATATTCTCGCTGTTCTGATTTTGTAATAGTCAGGACAGGCTAAACATTCGCTATATTAAGACCATGCATGTGTCCCCAAACCTAGTTCTTTCCCTAGGTCTGGTTTTATAAATGCTGGTGATAAAC
Oa2133 / 1 / contig33729
2470-2610(+) / EU093993:
cCCGCCACCACCGCCACTATACCTGTCTTAAATCCAAGTGACTGGTATTTACCAGTGGTAGGCTTAGAAGAATCTCCAGCTGTaggaagcaggcctctcctggctcatggagagctctgtgccgaaaggctggtaacacga / /
bOa
HACA33 / ultra520
1654200-1654334(+) / EU093995:
taagccagtatacttcatctgcttaactgaacgtgttttgcagaagggatgtgtgaatatgctggcaatagcatagcctgggtacgagccttgaagactctgtagtcattaaggtccctgaaattggcaacatct / H/ACA box 33 / NR_002436
chr.6 / cAAGCCAGCCAATGAATCTGCTTACCTGATTGTGTTTGTGCAGACATACTTTAAAAACTGGCAATAGTAAAGCCATGTTACGAGCCTTAAGGACATTGAAGTCGTTAAGGTCCCTGAGAATGGCTATAACA
bOa
HACA62i / ultra117
1693406-1693566(-) / EU094012:
cccgcacacggttagagcttggagctgaggccggtctccggccgatgaactaccgagtgtaggtcgtgtgccacagcgaaggacggcgggttttttcgctaacgccacgaggccctcggtgggccgacgagtggagtttgatagtgacatctgccacaggt / /
bOaHACA
70 / ultra401
7215-7353(-) / EU093994:
ttatccgcagtcaattaagccgaccggcccttgtctccatcaggggtcggtgtgcaatggctgcggacagcagtcctccctggttgtgtatgctccccgttccctgtacggggtgctctaagggaccttggagacatcc / H/ACA box 70 / NR_000011
chr.X / CCGCAGCCAATTAAGCCGACTGAGTTCCTTTCCTCATGGGGACCCAGTGTGCGATGGCTGCACACAGCAGCTTCCTTGGTAGTGTACGCAGCCTGTTGGTTGTATGGGTTGCTCTAAGGGACCTTGGAGACAGGC
Table S 13: Full-length H/ACA-box snoRNAs in introns of ribosomal protein genes (group III: specific for platypus)
Ornithorhynchus anatinus
name / numb. of seq. clones / Sangergenomic location / NCBI Acc-No and sequence
Oa1858
/ 6 / contig278044(-)182-317 / EU093997:
tAAGCCAGTATACTTCATCTGCTTAACTGAACGTGTTTTGCAGAAGGGATGTGTGAATATGCTGGCAATATCATAGCCTGGGTACGAGCCTTGAAGACTCTGTAGTCATTAAGGTCCCTGAAATTGGCAACATCTC
Oa1968 / 1 / ultra659
12300-12438(+) / EU094036:
ACTGCACCCCCAAGCCCCAAAGATCATTGTTTCCTTCTTCACAATGATCTTATCTGGGGTGCTGACATCAATTGTACCTCGTCGCCTTTCAAGAGCTGTTCGAAGTGCTCCTGAAAGTGAAATGGAAGGTTCAACATAT
Oa2161 / 2 / ultra401
6423-6558(-) / EU093998:
CCCCGCAGACAATTAAGCCGACTGGCCCCTGCCTCTGTCGAGGGCCAGTGTGCAATGGCTGCGCACAGCAAACTCCTCGGTTGTGTACGTGCCCCGTTCCCTGTATGGGGCGCCCTAAGGGACCTTGGAAACAGCA
Table S 14: Full-length H/ACA-box snoRNAs in introns of genes other than ribosomal protein genes (group I: conserved in vertebrates)
Ornithorhynchus anatinus / Homo sapiensname / numb. of seq. clones / Sanger
genomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1744
/ 1 / ultra443356772-357023(+) / EU093999:
aTAACATGCTTCCTTAGATCCACCTttgtggttgcccaccttgaactgagttccgccagttaaactctttgtttctggtagttccagtcaagaggcatccggcaacttctttggttgtacagtcaaaggtgcttgagtcattggcatgtaagagaaatatacctgcatgttagtcatacgttctgattgcaagtgacagcagcattgcgtgtcatttctcacgatcagagcctgactggtgtgcggacactt / H/ACA box 53 / NR_003015
chr.12 / AACATGCTTCCTTAGATCCACCTTTGTGGATGAATCTTGAACTGAGTTCCACTTGTAAACTTCTTGTTTCTTGTGGTTCCAGTAGTCAAAGAAACATCCAGCAACTTTTTTGGTTGTATAGTCAAAGGTGCTTGAGTCATTGGCATGTAAGAGAAATATACCTGCATGTTAGTCTAACGTTCTGATAGAAATGACATGCATTTATGCTGCCATTTGTTACTATCAGGACTCGACTCGTGTGCGGACATTT
Oa1860 / 1 / Contig704
69996-70133(+)
1mm:
G in cDNA seq
A in genomic seq / EU094001:
acCGCACCTGAATCTTTCCCGTGCCTTGCTGGGCAGTGTGGGTTTGGGGACAGATGGTGCTACACTAGCTGCAGAGGAAATCCAGGCAGGTTACTTTCCATTTacattgcaacttgcctctacaactctgccacattt / H/ACA box 55 / NR_002983
chr.1 / GAGCACCTGAATCTTTCCCATTCCTTGCTGCCTCGTGCCGGTGTGGGGACAGATGGTGCTACAGAATGAGCAGAGGAAATCCAGACAGGTTGTTTTCCATTTGTCTTGGGGCCTGTCTCTACAGCTCTGCCACATTT
Oa1867 / 12 / ultra336
7054603-7054735(+) / EU094002:
aACTGCATCTCTTTGACAGGCCTGGGGCAGTTCCATCTGCTTTCGTGGCTCCCACTAAGATGCAAGAAAAAATTCCTCGCGCTTTCTGCCTGCTTGATCTGTGGCAGTCCAGATTGAATAGAGGAAGACAAGT / new H/ACA / Chr.13
NT_024524
26890584-26890449 / AAATGCATCTATTTGACAGACCTGGAGCAGTTGCTATCTGCTGCTATGGTTTCCACCACAGATGCAAGAAGAACATGTCCTTGCGCTTTCCGTCTGTCTAATTGTGGCAGCTGAGATTGAATAGAGGAATACAGGA
Oa1870 / 4 / no 100% match in Platypus-BLAST / EU094003:
ACGCAGGAACTAGTTACAATGTGGCTGTGTGGTGCCGTGTTGTCGTTCCCCCTGCTGAAAGCAAAACGTGTCTCTTTCTATCCCTGTCCGCCTCGATCTACTCCCGGCGGTCAGGGACGCTTGGCTTCACACATGC / H/ACA box 63 / NR_002586
chr.3 / AAAGCAGGATTCAGACTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATAAAGTTGTTTCTTAACTATACCTGTCTGCTATTCTCCTGTAGCAGCCAGGGACGCTTGGTCTCATACATGT
Oa1949 / 1 / ultra336
7053708-7053839(+) / EU094004:
gcCCGCATCTACTGATAGACCGTGAACAGTTCTCCGTTGTTCTTCTGGTTTGCACAATGATGCGAAAGAACACTTCCTCGCACTTTCTGCCTGCCTTGCGTGGCCGCCCAGATTGAATCGGGGAGTACAGCT / H/ACA box 31 / NR_002967
chr.13 / ttCTGCATCCACTGATAGACCTTGAACAATTTACTGTTGTTCTTTTGGTTTGCACTAGGATGCAAAAGAAAGAAATCCCTGCGCTTTCTGTCTGTCTTTGTGGCGGCCCAGATTGAATTGGGGAATACATCT
Oa1957 / 2 / contig172642
496-626(+) / EU094005:
acCCAGCACTTTCACAGGTCCGCCTCTGGTCGTGGGGTCGGGATTTGGTAGCTGGTGCTGAGAGCAAAACCCTCGGGCACTTCCTCGCCCTGGCCTTGGGCCGGTGGCAAGAGTGATCGAAGGGGACAGAG / H/ACA box 30 / NR_002966
chr.16 / HACA 30:
CTGGCACTTTCACAGTTCCTTCCCCAGGCAGTGGGGCCAGGATTTGGTAGCTGGTGCTGAGAGAAAACCCTTGATTGTATTCTTGCCCTGGGATTATACCAGTGGCAACTGTCACTCAATGGGACAGTG
Oa1969 / 1 / ultra170
93312-93448(-) / EU094007:
cCTTCCACAGCTACTGGTCTGCAGCTGTTGTGCTCTGTAGCAGTTGCAGTATTCCATCTGTGGGAAAGAAACTTGTTGACACTGTTCGTTACCCCTTTCTCGGCAACAGAATGGGGTTTTCTGTGCGACAGACAAAT / HACA
box 41 / NR_002590
chr.2 / TTCCACAGCTACTGGTCTGCAGCTGTTCTTATGGTAGCAGTTGTGGCATTCCTCTGTGGGAAAGAAACTGTTAACACAAACACCTCTTTCTTAGCAAAACAGAAAGTGGGTATATATGTGTGACAGACACAA
Oa2002 / 6 / contig8068
29836-29975(-) / EU094017:
gGTGCATAGACGAATTCTGCGTTGGCTTGACTGTGGCCAGTGCAGTCAGACACTATGCTAAAGCATATTCTCTCCAGAAACACTGCTCGTGGTGTGCCGGATCCAGAGTTGGCACCATCCCAGAGAGAACGAGAACATGA / /
Oa2163 / 1 / chr.4
23442933-23443069(+) / EU094009:
GACGGTGATCTTCCTCATTCCTTTCTGGCACTTGAAACCATTGCCCAAAGGATCTCGAAGTCACCAAGAATAAGTTGGAGGTTGCCAAAGGCTTGTTTGAAAAGGTTTATCAAGCCCGACCGAGAACTCTGACATTT / new
H/ACA / Chr.14
AL137818
35515-35648 / AAAGGTGATTCTTTTTATATGCCCTTCTGATCCCAAGTATGGCAGAAGGATATAAAGGTCACCAAGAATAATTTGGAGGTTGCCAAGGACTTATTTAAAAGTGTTTTCAAGTCCGACCGAGAACTCTGACATTT
bOa
HACA22 / contig8068
31542-31676(-) / atcgcacagcgaacacccaagtgtgctctctagttctcttggtctaaattctgtgctaaagcaaacgttgttcttttcctctgcaatgaaaggagtccttttccttttatatgtattcagaaggacagcacacgg / NR_002961
chr.7 / TTGCACAGTGAACACCCAAGTGTGCTTTATAGTTCCCTTGGCTTTGACCCTGTGCTAGAGCATTGCCTGCTCTTCTCCTCTGCATTAAAAGGAATATTTATCCTTTTAAATGTATTCAGAAAGCCAGCACATTA
bOa
HACA28 / chr.1
22307816-22307950(+) / EU094010:
catgcaagtaacactctgtggcagacaccgttgtctgactcaattttgagcttgctataataaaaaaaaaaaagcctaacctattccagtggtctcttttaaatgagaccatctgttatatagactgtgacagtg / HACA
box 28 / NR_002964
chr.14 / AAGCAACACTCTGTGGCAGATGATCAAAACTGTCTGACACAATTTGAGCTTGCTATAGCAAGAAAGTCTAACCTATTCCGGTGTTCTCTCTCCCATGAGACAAGCCGTTATATAGACTTAAACAGT
bOa
HACA36A / ultra519; 9351033-9351168(-) / atctccaaagtgttgagttcaggtcagggtttctcccctgttctggtaatttaactttggaacattggtaatggctagaggaaatgaaagggtggagagtgttgctctacccgtttttaccacctagcgtacaacc / H/ACA box 36A / NR_002969
chr.x / TTCCAAAGTGTTGAGTTCAGTCCAGGGCAGCTTCCCTGTTCTGTTAATTAAACTTTGGGACATTAAAATGGGCTAAGGGAGATGATTGGGTAGAAAGTATTATTCTATTCATTTGCCTCCCAGCCTACAAAA
bOa
HACA56 / ultra519
9347326-9347456(-) / EU094011:
aaggcagatggttatccctctctagtctgatccagtgtctctagagggagtcagtctgcaaaatcaactgggggccctgtttccgtccagcatcatggttctttgatgatggtttcagacctgccatacat / HACA
box 56 / NR_002984
chr.X / CGGCAGACAGTTATCCCTTTCTAGTCTGGCTCGTGGGACTCTAGAGGGAGTCAGTCTGCAACAGTAAGTGGTGAGTTCTTCTGTCCAGCGTCAGTATTTTGATGGTGGCTTTAGACTTGCCAGATAACA
bOa
1860i / contig704
68731-68867(+) / EU094013:
atcgcacctaaatctttcccactcctgtgaccccgtgcgggagtggtgacaaatggtgctacagaatggcagaggaaatccagacaggtcgctacaccaatcatccaggcacctgtccctacaactctgcaacatgt / /
bOa
1964i1 / ultra403
1293229-1293368(+) / EU094014:
gcctgcatggcattggatttatggcaggtcccactctgtgagcctgtcatagtgtactccatgcaagagcaaacagccatcccgaaccattgcccggtcgctgcccgtaggccgagggcactgaagtggatggaacagct / /
bOa
1964i2 / ultra403
1296313-1296452(+) / taaggcacagtttggatttatggcaggctcagtcccatgggcccctcatagtgtggactgtgccagagcaacaaccacccaaaaccattgcctggcctctgtctccgtaggccgcaggcactgatatgggtggcacaaca
Table S 15: Full-length H/ACA-box snoRNAs in introns other than ribosomal protein genes (group II: conserved in mammals)
Ornithorhynchus anatinus
/Homo sapiens
name / numb. of seq. clones / Sangergenomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1809
/ 17 / ultra258257787-257923(-) / EU094015:
tCTTCCTCAGCCTTACTCCAGGGGCTTTCTTGCTCATAGAGTGCCCTGGCATTGCCTGAGGATAAATCAAAAAGCCCATATCCCTCCCCAGTAGGAGCCTGATTTTCCTTTGGGGCCTACAGTTTGGGCTGACACAG / H/ACA box 49 / NR_002979
chr.12 / CTTCCTCAGCCTTACTCCAGGGACTTTTGTTGCCTGTAAAGTGCTCTGGCATTGCCTGAGGATAGATGAGAAAGCACATATCCCTCCCCAGTAAGACGCTGTTTTCTTTTGGGGCCTACAAGTTGAGCTGACAGTA
Table S 16: Full-length H/ACA-box snoRNAs in introns of genes other than ribosomal protein genes (group III: specific for platypus)
Ornithorhynchus anatinus
name / numb. of seq. clones / Sangergenomic location / sequence
Oa1849
/ 3 / ultra72713634-13772(+)
1mm:
C in cDNA seq.
T in genomic seq. / EU094000:
cCTGCATTCTTAAACCCTCTTGGTAGCGTCGCCAGAAGCTGCCAAGATACGAGTGAATGCGACAGGAAACTGCAGATCAATCCAGAATGCCGGGCTTGGCTCCTGTGGCCTGGCATTCTTTCACTAATCTGAAACAGCT
Oa1964
/ 1 / ultra4031297085-1297223(+) / EU094006:
CAGGCATGGCACTGGATTTGTGGGCAGGCCTAGCCCCGTGGCCTGCTCATAGTGTACTCCATGCAAGAGCGACCAGCTGCCCCGAACCATTGCTCGGCCTCTGCCCGTAGTCCTGCGCACTGAAGCGGGCAGCACAGCT
Oa1967
/ 1 / chr. 122311096-22311226(+) / EU094016:
TATGCAAGTAACACTCTGTGGCAGACAATGTTGTCTGACTCAATTTTGAGCTTGCTATAGTAAAAGAAAGGCTAACCTATTCCAGCGGTCGCTTTCAAGTGAGACTGTCTGTTATATAGACTTTGACAGGG
Oa2092 / 1 / ultra519
9348949-9349082(-)
1 mm:
C in cDNA seq
T in genomic seq. / EU094018:
gAAGCAGGCGGTTATCCCTCTCTAGCCTGATCGGGTGGCTCTAGAGGGAGTCAGTCTGCAAAATCAAATGGAAGGCCCTGTTTCTGTCCAGCACCACAGTGCTTTGGTGGTGGTTGCAGACCTGCCATATATgg
Table S 17: Full-length H/ACA-box snoRNAs in intergenic regions (group I: conserved in vertebrates)
Ornithorhynchus anatinus
/Homo sapiens
name / numb. of seq. clones / Sangergenomic location / NCBI Acc-No and sequence / ortholog in human / NCBI
Acc-No / sequence
Oa1813
/ 2 / ultra292670761-2670878(-) / EU094019:
tCTGGTCATTACCAAGGCTATTAGAAGCACAGTTTCTGATTTGCTGTGGACATGACCGTAGAATAACTCTCTCGGTTTTAAATGGCTATATTATTAGCACAAACCTGGAGAGACATGC / H/ACA box 32 / NR_003032
chr.11 / TGGTCATTACCAAGGCTTTTAGAATGCAGTTTCTCATTTGCTGTGGACATGACCATAAAAAAATTTCCCAGTAGGTTTTCTATCTGCTACTTTGCTAGCAATCAGCTTATTGGGAACAGTT
Oa1844 / 10 / ultra29
2675081-2675210(-)
1 mm:
T in cDNA-seq is missing in genomic seq. / EU094020:
aCTAGCACTTCTCTTGTTCTTGTTCAATCTGGGGACGAGGACTTAGAAA(T)AAGTGCTCAGAACAAACCCTCTTTTGCAACCCACAGTTCATGGCACAGTATGAGCTGTGGTACATATAAGGAGGGACACCT / H/ACA box 40 / NR_002973
chr.11 / TGCACTTATGTATGTTTTTGTTTAACGTGGACAAAGACTTACAGATAGGTGCAAAAAATAAATCCTCTTTTGCAACCCAGAACTCATTGTTCAGTATGAGTTTTGATACATATAAGAAGGGATATTA
Oa1861 / 1 / Contig23414
2813-2943(-) / EU094021:
gACCCCTTTAATTGTGCTCCCTTAGAGCTGCTTGTGCTCTAAGGGGCAACACAGGAGGGAATAACATTCCTCTCTTAAACCCAGAGCTGAGGCTAAAAACTCTCCTCTGGATCCTGTCGGGAGGCACACTG / H/ACA box 26 / NR_003016
chr.4 / GTGCCCTTTTAAGGTTGACCCAGTGCTTTAAGAGGCTAACACAGAAGGGTAAAGTAAGTCTCCATAAAACCCAGAGAAGAGACTGGAAAGCTCCTCTTTGGATCCTGTCTGGAGTCACAACT
Oa1886 / 5 / UltraContig84: 2069663-2069796(-) / EU094022:
TTTGCCTTTTGTGTTGCCCGTTCACTTTTGGCTGCTAGTGAATGTGGTGTCAAAAAAGGCGCAAATCAAATCCGTGGCAGCCCTATTTTCACTGGATTCTATTCCTGTGGTGTACTTGCTGCACAGGAACATGG / H/ACA box 13 / NR_002922
chr.5 / AGCCTTTGTGTTGCCCATTCACTTTGGAAACTAGTGAATGTGGTGTCAAAAAAGGCGTAAATTAAACGCTTTGCAGCCTTTTCCTGCCCTTAAATTTGATACCTTTGGTGTAGGAGCTGCATAAGTAACAGTT
Oa1959 / 1 / Contig24898
7264-7393(-) / EU094023:
ccACCCTCTGGATCCCTTTCCCATCAGGTCTGCACACCGACCTGGGTGGTCGTAAATGGAGGGAAAGGAACTGGATGCGGGCCCGAGTGTCTGGTGTGGAGACAGCGGACCAGGCCCGGTCCTCACAAtc / H/ACA box 61 / NR_002987
chr.1 / ATCCTCCTGATCCCTTTCCCATCGGATCTGAACACTGGTCTTGGTGGTCGTAAAAGGAGGAAAAGTAATAGTGAAGCTGGCCTAAATGTTGTAATCTGGTATATGGCATGTGGGCTAGTTTCAGACAGGT
Oa1962
/ 1 / ultra292672281-2672423(-)
1mm
C in cDNA seq.
T in genomic seq / EU094024:
gCTGCACTGTATGGCATCTACACCCAGCAGTCTTTTTCCTGGTGGGGTGTTCAAAAGCCAGTGCTAAAGGAATGCAATATCTGGCATCGTTGGTTTCCTTGCCGTTCGTGCTTGTTAAACCTGGTATTTGTATTGCTACAGGC / H/ACA box 8 / NR_002920
chr.11 / TGCACTGCATGGTATCTGCACTCAGCAGTTTACACCTGCTAGGGTGTTCAAAGGTCAGTGCTATAGAAATTCAGTATCTGGCATCGTTGGTTTTCTTGGCTTTGTGCTTGTTAAACCTGGTATTTCTACTGATACAGTA
Oa2001 / 4 / ultra29
2674266-2674400(-) / EU094025:
ggctTTTGAGGTCTATCCCGATGGGACCTCTCATAGGTCCACGTCGTTGGAAACGCCTCAGAGAAAAAAGTTGTGGTCTCGCTTTACCTACCGAACTGTGTGTTGGATCGGTGGGGGAAAAGCCACAAGACAGTT / HACA
box 18 / NR_002959
chr.11 / GTTGAGGTCTATCCCGATGGGGCTTTTCCTGTAGCCTGCACATCGTTGGAAACGCCTCATAGAGTAACTCTGTGGTTTTACTTTACTCACAGGACTATTGTTAGATCTGTGGGAAGGAATTACAAGACAGTT
Oa2086 / 1 / no 100% match in Platypus-BLAST / EU094026:
CTTATCGAAGCTGCCCCGCTCCGTCCGTGGCGGGTGTAGTCAAAAAGGAGCCCAAAGTGATCTTTTCCTCGACGGTCGCTGTTCGGTTTGCGGTAACCGGCCCGCAACATTTCGGGAAAAGACATGACAC / H/ACA box 16A / NR_003035
chr.1 / TTGGCCCTTATCGAAGCTGCAGCTGCTTCCGCATAGCTGCTGTGGTCAAAAAGGAGCCCAGAGTGACAGTTTTCCTTGACGGTCGCCGTTCTGTTTGTTGTAACTGATCTGCAACATTTTGGGAAAATACAGTT
Oa2150 / 1 / no 100% match in Platypus-BLAST / EU094027:
CCTCCATGACACCTTGGGACCTGCCTCTTGTGGCGGCCTCCCTTCCTAGGCATGGAAGAGCATCAGCTTGTTGATGGCAAAGCTGCCGCCATTGCATTGGTCGCAGATTCTGACTTGTGCAAGCGACAGAAC / H/ACA box 85 / AM413010
chr.15 / H/ACA 85:
CTCCATGTATCTTTGGGACCTGTCAAGTGTGGCAGTCTCCCTTCCTTGCCATGGAAGAGCATATTCTTGTTTACCAGCAAAGCTGTCACCATTTAATTGGTATCAGATTCTGACTTGCACAAGTAACAT
Oa2164
/ 1 / contig852623420-23553(+) / EU094028:
CGACGCCGTCTGAAAGTCGCCCGCGGCCTGACCCGTGGCCGCGAGTGAGACCCCGGCGCCCACAGACACAGGGGCCTAAAGCTTGTCGCCCACCGCCCTGTCGTGTCGGCGACAAAACCCGGGCCCCGACAGGC / H/ACA box 78 / NR_003020
chr.16 / GTTGGTTGAAAATCGCCCCCGGCTTTGGCCGTGGCCGCGGGTGAGATTCGGCGCCCAGAGCCCCCGGGGGCCTCAGCTCACCGCGCGCTGCCCCATGTGCGGCGGTGAAACCCAGGCCCCGACAGGC
Oa2167 / 1 / ultra29
2670484-2670615(-) / EU094029:
aAGAGGTCATTTCAAAGAGGGTTCGTATGGCTGTAAAACCAGCAGCCCTTAACGCGATGACCTAATAGGAACTCTCTACAGGTTACCACTCTCACTAAGTAGTGCACTGTGGTTTCCTCCTGAGACACAGTG / HACA
box 25 / NR_003028
chr.11 / GGGTCATTTCAAAGAGGGCTTATGAGGCTGTGAAACCCAGAGCTCTTAACGCTGTGACCAAAGATGGAAGTTCTCTATAGGAAGCCATAGCACTCCTAATGTTTGGTGCTATGTTTTCCTGAGGAGATATAAAA
Oa2181 / 1 / ultra157
392187-392322(-) / EU094030:
tggTGCCCCTCGAGGCGTTGCAGATGTGGCTGCGGTGCCCCATTTGTGTCATGAGGTGGCAGAGAGGATAAGGCTATGTCTACGCTCCGTGTTCAAAAATTTGTGAGCACGCAGAATGATTAATAGCCTGACAGTT / H/ACA box 17 / NR_002958
chr.9 / ACTGCCCCTAGAGGCGTTGCAGCTGTGGCTGCCGTGTCACATCTGTGTCATTAGGTGGCAGAGATTAGAGAGGCTATGTCTACGCTCAGCGTTCTGCCCCGTGAACGTTTGAATGTTTGATAGTCTCACACTC
bOa1886i / ultra43
553650-553784(-) / EU094031:
tttgccttttgtgttgcccgttcatttttggttactgatgaatgtggtgtcaaaaaaggcgcaaatgaaactcttggcagctctgtttcaccctaggtttccgttcctttggtgcttttgctgcgaggaacatat / /
bOa2150i / chr.2
37937690-37937820(-) / EU094032:
tctccatgacaccttgggacctgcctcttgtggcagcctcccttcctaggcatggaagagcatctgcttgttgatggcaaagctgccaccatttcattggtagcagattctgacttgtgcaagcgacaagg / H/ACA box 24 / NR_002963
chr.4 / ATCTCCATGTATCTTTGGGACCTGTCAGCCGTGGCAGTCTCCCTTCCTAGCCATGGAAGAGCATATCCTTGTTTATTGGCAAAGCTGTCACCATTTAATTGGTATCAGATTCTGACTTGCACAAGTAACATTC
bOa2164i / contig11953
18277-18410(-) / EU094033:
cgacgccgtctgaaagtcgcccgcggcctgacccgtggccgcgagtgagaccccggcgcccacagacacgggggcctaaagcttgtcgcccactgccctgtagtgtcggtgacaaaacccaggccccgacaggc / /
bOa2181i / ultra157
391426-391561(-) / EU094034:
gtatatgcccctagaggcgtacagatggggctgctgtgcctcatttgtgtcatatggtggcatagagcaaaaggctatgtctacgctcggtgttctcagtccatgaacactcagaatgattagtagcctgacaatg / /
bOaHACA1 / ultra29
2671791-2671928(-) / cctgccccattccaaatattcatggacacagttcatgtttgtgtcacatggaacaatgtggctacagtgaaagcacaggttttgcttccatcacagtaaaaataagttactctggtgacaatgagacctgtaacagga / H/ACA box 1 / NR_003026
chr.11 / GCCTCATTCTAGAGAATGGGCACTGTTGATCATGGTGTCCAAAAATAGTTAATGTGGCTAAATTGAGACAGGTTATGCTTCCATCACAGTATGCATATTGCAGTGGTGACAATGAGACCTGTAACATTT
bOa
HACA44 / contig24898
7618-7753(-) / ctcggcatgttcttcaagggccgaggctggacgcagtagcctcggcgtctccaactgcatgccacaacagcctggagagactttgacagcgcagagcctgccgtgcctggtgctgccactcggctctcccacagtt / H/ACA box 44 / NR_002976
chr.1 / CAGCATGTTTCCAAGGGCTGTGGCTGGTCATAGCCATGGGATCTCCAACTGCATGCAAGAGCAACCTGGAAAGACTTTGACAGCGCAGGTCAGTACAATACCTGCAAGCTGCCACTCAGCTTTCCTATAATG
Table S 18:Full-length H/ACA-box snoRNA, genomic locus not assignable (group III: specific for platypus)
Ornithorhynchus anatinus
name / numb. of seq. clones / Sangergenomic location / NCBI Acc-No and sequence
Oa1965 / 1 / no 100% match in Platypus-BLAST / EU094035:
CCCCTCCCACAAAGGCGTGTCTGCAGCCCCGTGTGCCGGCGCGTAAGAACCGGAGGCAGAGAGGCAGGGACGGGAGCGTCCCGGGGGCGACTGGCTCCCTCGTGGGCCCCGGGACCACCCCCCGTCCTACAAGGC
Table S 19: intronic full-length snoRTE H/ACA-box snoRNA (group III: specific for platypus)
Ornithorhynchus anatinus
name / numb. of seq. clones / Sangergenomic location / sequence
Oa1862
/ 1 / ultra27741027-41164(+) / EU094037:
ggTCCCTCAGCGTCGCACCTGGAGAGTTCCCAGTACTCTACCGGTCTCGGCAACGGGAGGGAGAGTCAAACAGAGGCCCGTCCAGTCCATTCCTAGCTTGGGCAGTGGCTAGCGAGTGGCAGGCCATCGGCTACAAGT
Oa1864 / 3 / contig107178
452-591 / EU094038:
aCCCCCTCTCAGAGTCACACGTGGAGAGTTTCCAGTACTCTACCGGTCTCGAATACTGGAGGGAGAGTGAAGCAGAGGCAGATCCATTCCATTACTAGCTTGGGGCAGTGGCTAGTGAGTGGAAGACAATCTGCTACAAAT
Oa1874a / 1 / contig10981
2130-2267 / EU094039:
CCCCCCTCAGCTTCGCACCTGGAGAGTTTCCAGTACTCTACCAGTCTCGGCTAGGGGAGGGAGAGTGAAGCAGAGGCCTACCCATTCCATTCCCAGCTTGGTCCAGTGGCTGGCGAGTGAAGACAATCTGCTACAGGT
Oa1912 / 7 / chr.1
20306401-20306540(+) / EU094040:
aACCCCTCTCAAGGTCACACCTGGAGAATTTTCAGTATTCTACCAGTCTCGACTCTGAGAGGGAGAATCAAGCAAAGGCATACCCATTCCATTCCTAGCTTGGTCAGTGGCTAGTGAGTGGAAAGCAATCTGCCACAAGT
Oa1954a / 1 / chr.2
6474254-6474394(-) / EU094041:
gACCCCTCTCAGGGTCGTACCTGGAGAGTTTCCAGTACTCTACCAGTCTTGACTACGGGAGGGAGAGTCAAGCAGAGGCCTATCCATTCCATTCCTAGCTTGGGCAGTGGCTAGTGAGTGGAAGGCAATCCTGCTACATGT
Oa1954b / 3 / chr.X1
44718101-44718243(+) / EU094042:
tcCCCCTCTCAGGATCTCACCTGGAGAGTTCCCAGTACTCTACCAGTCTCGACTACAGGAGGGAAAGTCAGGCAGAGTCATATCCATTCCATTCCTAGCTTGGGCGGCAGTGGCTAGCGAGTGGAAGGCAATCTGCTACAAGT
Oa1954c / 1 / chr.7
35826545-35826684(+) / EU094043:
agCCCCTCTCAGGGTCGCACCTGGAGAGTTTCCAGTACTCTACCAGTCTCGGCTCCGGGAGGGACAGTCAAGCAGAGGCCTACTCATTCCATTCCTAGTTTGGGCAGTGGCTAGCGAGTGGAAGGCCATCTGCTACAAGT
Oa1954d / 1 / ultra497
89564-89703(-) / EU094057:
agcccctctccaagtcacacctggagagttcccagtactctaccAGTCTCGACTGCGGGAGGGAGAGTCAGGCAGAGGCATACCCATTCCATTCCCAGCTCGGGCAGTGGCTAGCGAGTGGAAGGCAATCTGCTACAAGT
Oa1956a / 3 / chr.4
47749188-47749327(+) / EU094044:
gtCCCCTCTCAGGATTGCACCTGGAGAGTTTCCAGTACTCTACCAGTCTTGGCTATGAGAGGGAGAGCCAAGCAGAGGCCTACCTATTCCATTCCTAGCTTGGGCAGTGGCTAGCGAGTGGGAGGCAATCTGCTACAAGT
Oa1966
/ 6 / ultra5461001642-1001781(-) / EU094045:
cCCCCCTCTCAGAGTCACACCTGGAGAGTTTCCAGTACTCTACCAGTCTCGACTATGGGAGGGAAAGCAAAGCAGAGGCCTACTCATTCCATTCCTTCCTTGGGCAGTGGCTAGTGAGTGGAAGGCAATCTGCTACAAGT
Oa2149
/ 2 / contig71091835-91974(+) / EU094046:
atCCCCTCTCAGCGTCTCACCTGGAGAGTTTCCAGTCCTCTACCAGTTATGACTACAGGAGGGAGAGTCAAGCAGAGGCCTGTCCATTCCATTCCTAGCTTGGCCAGTGGCTAGTTAGTGGAAGACAATCTGCTACAAGt
Oa2166 / 1 / contig144102
416-554 / EU094047:
tAACCTCTCAGGGTCGCACCTGGAGGGTTTCCAGTACCCTACCAGTCTCGGCTATGGGAGGGAGAGTTAAGCAGAGGAATACCCCTTCCATTTCTAGCTTGGCCAGTGGCTAGCAAGTGGAAGACAATCTGCTACAAGT
Table S 20: Full-length snoRTE HACA-box snoRNA, genomic locus not assignable (group III: specific for platypus)
Ornithorhynchus anatinus
name / numb. of seq. clones / Sangergenomic location / NCBI Acc-No and sequence
Oa1874b
/ 2 / contig809519849-19987(+) / EU094048:
tCCCCTCTCAGGGTCACACCTGGAGAGTTTCCAGTACTCTACCAGTCTCAGCTACAGGAGGAAGAGTCAAGCAGAGGCATACCCATTCCATTCCTAGCTTGGCCAGTGGCTAGCGAGTGGAAGGCAATCTGCTACAAGT
Oa1956b / 1 / contig18346
8038-8175(-) / EU094049:
taCCCCTCTCGGTCGCTCCTGGAGAGTTTCCAGTACTCTCTAAGTCTCGGCTATGGGAGGGAGAGTCAAGCAGAGGCCTACCCATTCCATTCCTAGTTTGGGCAGTGGCTGGCGAGTGGAAGGCAATCTGCTACAAGT
Oa1972a / 2 / no 100% match in PlatyBlast / EU094050:
CCCCCCTCAGCTTCGCACCTGGAGAGTTTCCAGTACTCTACCAGTCTCGGCCAGGGGAGGGAGAGTGAAGCAGAGGCCTACCCATTCCATTCCCAGCTTGGCCAGTGGCTGGCGAGTGGAAGACAATCTGCTACAGGT
Oa1972b / no 100% match in PlatyBlast / EU094051:
CCCCCCTCAGCTTCGCACCTGGAGAGTTTCCAGTACTCTACCAGTCTCGGCTAGGGGAGGGAGAGTGAAGCAGAGGCCTACCCATTCCATTCCCAGCTTGGCCAGTGGCTGGCGAGTGGAAGACAATCTGCTACAGGT
Oa2040 / 2 / no 100% match in PlatyBlast / EU094052:
ACCCCTCTGAGTCGCACCTGGAGAGTTTCCAGTACTCTACCAGTCCCATCTGCAGGAGGGAGAGTCAAGCAGAGGCCTACCCACTCCATTTCTAGCTTGGCCAGTGGCTAGGGAGTGAAAGGCAATCTGCTACAAGT
Oa2050b / 1 / Contig7525
5924-16061(+) / EU094053:
tCCCCTCAGGATCGCACCTGGAGAGTTTCCTTTACTCTACCAGTCTTGGCTACGGGAGGGAGAGTCAAGCAGAGGCCTACCTATTCCATTCCTAGCTTGGGCAGTGGCTAGGGAGTGGAAGGCAATCTGCAACAAGT
Oa2052 / 1 / Contig3398
4925-5063(+) / EU094054:
tTCCCTCTCAAGGTCACATCTGGAGAGTTTCCAGTACTCTACCAGTCTCGACTATGGGAGGAAGAGtcaaggagatgcagacccattccatttctagcttggtcaatggctagagagtggaagacaatctgctacaagt
Oa2053 / 1 / no 100% match in PlatyBlast / EU094055:
CACCTCTCAAGGTCACACCTGGCGAGTTTCCAGTACTCTACCAGTCTCGACTGTGGGAGGGAGAGTCAAGCAGAGGCCTACCTATCCATTCTTAGCTTGGCCAGTGGCTAGCGAGTAGAAGGCCATCTGCTACAAGT
Oa2077 / 1 / no 100% match in PlatyBlast / EU094056:
CCCCTCTCAAGGTCGCATCTGGAGAGTTTCCAGTCCTCTTCCAGTCTCGGTTATGGGAGGGAGAGTCAAGCAGAGGCCTACCCATTCCATTCCTAGCTTGGCCAGTGACTAGTGAGTGGCAGGCAATCTGCTACAAA
For Table S21:
Column 1: Experimentally identified RNAs are indicated by Oa and a contig number.
Column 2: The number of sequence-clones presented in the cDNA library.
Column 3: Name of RNA.
Column 4: Sanger genomic location.
Column 5: spliceospmal RNA full-length sequences. Capital letters represent sequences from the cDNA library. Lower case letters represent genomic BLAST sequences. cDNAs are extended to presumed full-length (compared to human).
Table S 21: Full-length U1-U6; U7, U8, U11- U13 cDNAs
name / numberof seq. clones / RNA / Sanger
Genomic location
/ sequenceOa4000 / 18 / U1-1 / ultra324
241465-241629 / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCCGCCATTGCACTTTGGGCTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTG
Oa4001 / 233 / U1-2 / no 100% match in Platypus-BLAST / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCAGCCATTGCACTCCGGTTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTG
Oa4002 / 96 / U1-3 / contig71523
813-976 / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCAGCCATTGCACTTGGGTTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTG
Oa4003 / 76 / U1-4 / 1) ultra324
267582-267745
2) contig203629143-9306
3) ultra324228940-229103
4) contig88272522-2685 / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCCGCCATTGCACTTGGGCTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTG
Oa4004 / 2 / U1-5 / no 100% match in Platypus-BLAST / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCCGCCATTGCACTCGGGCTGTGCTGACCCCTGCGATTGCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGGCTGCGTTCGCGCTCTCCCCTTA
Oa4005 / 52 / U1-6 / 1)ultra324
271978 - 272141
2)contig41309
4983-5146 / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCCGCCATTGCACTCGGGCTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTG
Oa4006 / 339 / U1-7 / 1) contig67940
126-289 (+)
2) ultra324
270087-270250
3) ultra324
230514-230677 / ATACTTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCAGCCATTGCACTCCGGCTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTG
Oa4007 / 2 / U1-8 / no 100% match in Platypus-BLAST / ATACCTACCTGGCAGGGGAGACACCATGATCACGTAGGTGGTTTTCCCAGGGTGAGGCTCAGCCATTGCACTCCGGCTGTGCTGACCCCTGCGATTTCCCCAAATGCGGGAAACTCGACTGCATAATTTGTGGTAGTGGGGGACTGCGTTCGCGCTCTCCCCTA
Oa4008 / 34 / U2-1 / contig18615
536-720 / ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCCGGGAGATGGAATAGGGGCTTGCTCCGTCCACTCCACGCATCGACCTGGTATTGCAGTACCTCCAGGAACGGTGCA
Oa4009 / 20 / U2-2 / 1) contig72942
4134-4318
2) contig6367
323-507 / ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGATTTTTGGAGCCGGGAGATGGAATAGGGGCTTGCTCCGTCCACTCCACGCATCGACCTGGTATTGCAGTACTTCCAGGAACGGTGCA
Oa4010 / 16 / U3-1 / no 100% match in Platypus-BLAST / AAGACTATACTTTCAGGGATCATTTCTATAGTATGTCACTAGAGAAGTGAGAATGAAAGTGTGAAGAGACGAAAGCCACGAGGAGGAGTTTGAGTGTTCTCTCCTGAGCGCGAAGCGGGCTCGCGGTGCTGTTTTGAGCAGCTGCCGTTTGCCATTGATGACCGTTCCTCCTTCTCTTCGGGGAAGGTTGGAGGGAGAGAACACAAGCTGAGTGGTA
Oa4011 / 14 / U3-2 / no 100% match in Platypus-BLAST / AAGACTATACTTTCAGGGATCATTTCTATAGTATGTCACTAGAGAAGTGAGAATGAAAGTGTGAAGAGACGAAAGCCACGAGGAGGAGTTTGAGTGTTCTCTCCTGAGCGCGAAGCGGGCTCGCGGTGCTGCTTCGAGCAGCTGCCGTTTGCCATTGATGACCGTTCCTCCTTCTTTTCGGGGAAGGTTGGAGGGAGAGAACACAAGCTGAGTGGT
Oa4012 / 2 / U3-3 / no 100% match in Platypus-BLAST / AAGACTATACTTTCAGGGATCATTTCTATAGTATGTCACTAGAGAAGTGAGAATGAAAGTGTGAAGAGACGAAAGCCACGAGGAGGAGTTTGAGTGTTCTCTCCTGAGCGCGAAGCGGGCTCGCGGTACTGCTTTGAGCAGCTGCCGTTTGCCATTGATGACCGTTCCTCCTTCTCTTTGGGGAAGGTTGGAGGGAGAAAACACAAGCTGAGTGGT
Oa4013 / 2 / U3-4 / no 100% match in Platypus-BLAST / AAGACTATACTTTCAGGGATCATTTCTATAGTATGTCACTAGAGAAGTGAGAATGAAAGTGTGAAGAGACGAAAGCCACGAGGAGGAGTTTGAGTGTTCTCTCCTGAGCGCGAAGCGGGCTCGCGGTaCTGCTTTGAGCAGCTGCCGTTTGCCATTGATGACCGTTCCTCCTTCTCTTCGGGGAAGGTTGGAGGGAGAAAACACAAGCTGAGTGGT
Oa4014 / 23 / U3-5 / ultra324
232849-233064 / AAGACTATACTTTCAGGGATCATTTCTATAGTATGTCACTAGAGAAGTGAGAATGAAAGTGTGAAGAGACGAAAGCCACGAGGAGGAGTTTGAGTGTTTTCTCCTGAGCGCGAAGCGGGCTCGCGGTGCTGCTTTGAGCAGCTGCCGTTTGCCATTGATGACCGTTCCTCCTTCTCTTCGGGGAAGGTTGGAGGGAGAAAACACAAGCTGAGTGGT
Oa1766 / 26 / U4 c / 1) contig6023
12989-13133
2) contig6023
14483-14627 / AGCTTTGCGCAGTGGCAGTATCGTAGCCAATGAGGTTTATCCGAGGCGCGATTATTGCTAATTGAAAACTTTTCCCAATACCCCGCCATGACGACTTGAAATATAGTCGGCATTGGCAATTTTTGACAGTCTCTACGGAGACTGA
Oa1842 / 9 / U4 atac / contig239988
37-166 / AACCGTCCTTCTCTTGGGGTGGCGCTACTGTCCGATGAACGTTTAGTGAGGACAGTACTGCTAACGCTCAGGCAACACACTCGCACCTGCTAGGGCTCCGCCTTAACCGGGTGCAATTTTTGGAACCTTT
Oa3278 / 3 / U5-1 / contig2611
50679-50795 / ATACTCTGGTTTCTCTTCAGATCGTAAAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACGATTATGAGTTTGTAACTAATTTTTTGAGGCCTTGCTCCGGCGAGGCT
Oa3273 / 169 / U5-2 /
1) contig26893
9546-9662
2) contig176032879 -2995 / CCATATACTCTGGTTTCTCTTCAGATCGCATAAATCTTTCGCCTTTTACTAAAGATTTCCGTGGAGAGGAACAGTTATGAGTTTGTAACTAATTTTTTGAGGCCTCGTTTTTACGAGGCT
Oa2128 / 2 / U6 /
1) ultra428
215359-215466
2) ultra428179870-179977 / gtgctcgcttcggcagcacatataCTAAAATTGGAACGATACAGAGAAGATTAGCATGGCCCCTGCGCAAGGATGACACGCAAATTCGTGAAGCGTTCCATATTTTTA
Oa2466 / 6 / U7 / no 100% match in Platypus-BLAST / AGTGTTACAGCTCTTTTAGAATTTGTCTAGTAGGTTTTCCGGCGTTGGCTGGGAAGCCCTTC
Oa1948 / 5 / U8 / contig3446
43964-44101
1 mm:
A in cDNAseq
G in genomic seq / ATTATTGGTAGGTTATATCCTCACCTGTTTCTCCTTCGGGAGAGTGGATATATGATGATTGGAGTTCGCATGACACGTGATTAGTGTCTCCGCGACGATTGGGGAGCTGACAACACCCCAATTGTTCCTATCTGATTC
Oa2165 / 1 / U11 / contig29779
11220–11354
Parentheses for sequence not present in Sanger / AAAAAGGGCTTCTGCCGTGCGTGGCACACGGGAAGCGGCGGGGTCCCCTCAGCCGTTTCTCGTGCGGAATCGACATCAAGAGATTTCGGAAGCACAATTTTTGTCCCCGGGGCCGCTGGTGATCGCTTGTCCTGG(CGC)
Oa2180 / 1 / U12 / contig35671
6538-6687 / ATGCCTTAAACTTATGAGTAAGGAAAATAACGATTCGGGGTAACGCCCGAATCCTCACTACGCATGTGAGAAGAATTTTTGAGCGGGTACAGGTTGTCCCTAAGGTGACCCGCTTACTTCGCGGGATGCCTGGGTGTCGCGATCTGCCCG
Oa1793
/ 22 / U13 / chr.57566008-7566104 / atcctttcggttcattAAAAATGATGATTGGGTTGCCACGCGCCTGCGTGAGATGTGCCGCCCTTTTGTTACGCATGCGCACATTACCCTTCTGATC
For table S22
Column 1: Experimentally identified microRNAs are indicated by Oa and a contig number.
Column 2: The number of sequence clones presented in the cDNA library.
Column 3: Name of microRNA.
Column 4: Sanger genomic location and contig number.
Column 5: microRNA precursor full-length sequences. Capital letters represent sequences from the cDNA library. Lower case letters represent genomic BLAST sequences. cDNAs are extended to presumed full-length precursor (compared to human).Letters in bold case show the full-length microRNA.
Red letters: New microRNAs
Table S 22: Full-length microRNA cDNAs
name / numberof seq. clones / RNA / Sanger
Genomic location
/ sequenceOa1727 / 10 / miR 125b / contig534
330615-330702 / tgcgcccctctcaaTCCCTGAGACCCTAACTTGTGAtgtttcccgtttaaatccacgggttaggttcttgggagctgtgagttgtgcc
Oa1728 / 5 / miR 125a / no 100% match in Platypus-BLAST / TCCCTGAGACCCTTTAACCTGTG
Oa1772 / 5 / miR 128b / contig290019
29-110 / tgagccgctggattcggggccgatacacggtctgaggggtttacgagtcTCACAGTGAACCGGTCTCTTTTtcagttgctcc
O1819a / 4 / miR 100 / contig534
284952-285034 / agcctgttgccacaAACCCGTAGATCCGAACTTGTGGtgatattccacacaagcttgtgtctataggtatgtgtctgtgggcg
Oa1819b / 3 / miR 99a / ultra41
2045351-2045427 / agttggcataAACCCGTAGATCCGATCTTGTGgtgaagtgaaatacacaagctcgcctctctgggtctgtgtcagtg
Oa1832 / 4 / paralog to miR 100/99a / no 100% match in Platypus-BLAST /
CACCCGTAGATCCGAACTTGCGA
Oa1905 / 1 / miR 189miR 24 / contig22293
5777-5845 / cctccAGTGCCTACTGAGCTGATATCagttctcgttgacccactggctcagttcagcaggaacaggagt
Oa2099 / 1 / miR 221 / ultra222
5162752-5162846 / attcaggtttgaggcatgaacctggcatacaatgtagaattctgtatttgttaagcaacagCTACATTGTCTGCTGGGTTTCaagctacctggaa
Oa2100 / 1 / miR 16 / ultra336
5246367-5246441 / gtacctTAGCAGCACGTAAATATTGGCGttaagactctaaaagtatctccagtattgactgtgctgctgaagtaa
Oa2124 / 2 / miR 181a / chr. 4
46540188-46540277 / atggttgcttcagtgAACATTCAACGCTGTCGGTGAGTttggaattaaagtagaaaccatcgaccgttgattgtaccctgcggcaaccac
Oa2190 / 4 / miR 92b / no 100% match in Platypus-BLAST / TATTGCACTCGTCCCGGCCTC
Oa2273 / 1 / miR 148a / ultra231
1160632-1160701 / tgaggcaaagttctgtgacactcagactctgattatgatagaagTCAGTGCACTACAGAACTTTGTctcc
Oa2316 / 2 / miR 137 / chr 4
52507090 - 52507174 / gactctcttcggtgacgggtattcttgggtggataatacggattacgttgTTATTGCTTAAGAATACGCGTAGTCgaggagagta
Oa2350 / 2 / miR 30b / contig510
36011-36098 / accgagttcgagttcaTGTAAACATCCTACACTCAGCTgtgacccacccagcggctgggagggggatgtttgcttcaaccgacttgga
Oa2363 / 3 / miR 150 / contig7207
1845-1913 / gcccccTCTCCCAACCCTTGTACCAGAgcataaactgaacccctggtacggggccgtgggagaggggga
Oa2433 / 1 / miR 128a / contig290019
30-110 / tgagccgctggattcggggccgatacacggtctgaggggtttacgagtCTCACAGTGAACCGGTCTCTTTTtcagttgctc
Oa2551 / miR 146b / contig29185
8165-8234 / tggctcTGAGAACTGAATTCCATAGGCTGTgagttgatgaggatgccctagggattcagttccacagccc
Oa2569 / 1 / miR 135a / contig26295
4021-4106 / gccccgctgtgctcTATGGCTTTTTATTCCTATGTGAtcctgctgctcactcatatagggattggagccgtgcaacacggaggggg
Oa2577 / 1 / miR 145
miR RG-109-var1 / ultra 279
180929-181011 / accgtgtctcaggtccagttttcccaggaatacataggtgctgagatggggATTCCTGGAAATACtgttcttgaggtcatggc
Oa2597 / 2 / miR-26a / Contig8967
32520-32606 / gggctgcgacctggTTCAAGTAATCCAGGATAGGCTgtgtgcgctcagattggcctgttctcggttacttgccccagggggcggcca
Oa2619 / 1 / miR-126a*
miR-126a / ultra157
304113-304197 / agctggcaactgccCATTATTACTTTTGGTACGCGctgtgacgcttcaaactcgtaccgtgagtaataatgcgtggctggcagca
Oa2641 / 1 / miR 338 / no 100% match in Platypus-BLAST / CCAGCATCAGTGATTTTGTTGA
Oa2677 / 1 / miR 223 / contig3675
13366-13458 / cgcagtgccgcgctccgtgtatttgacaagctgagttggacactccgtgtggttgagtgtCAGTTTGTCAAATACCCTAagtgaggcattgct
Oa2811 / 1 / miR 91
miR 17
miR 18
miR 19a
miR 20
miR 19b
miR 92 / ultra284
6728094-6728180
ultra284
6728237-6728323
ultra284
6728451–6728534
ultra284
6728763–6728839
ultra284
6728879-6728951 / agactgcactcctcgcgatggcagacgacagtagcccc(miR91bprecursor)cgtcagaataatgt(miR91)caaagtgcttacagtgcaggtagtggtatgtagaatct(miR17)actgcagtgaaggcacttgtagcattatgttgacacctgccacagaaagcctcgttgggccttaacttgtacggtatgctggtttttcaag(miR18bprecursor)tgctttttgttc(miR18)taaggtgcatctagtgcagatagtgaagtagattagcatctactgccctaagtgctccttctggcataagaagttatgttttcgtccagtaactccaaatgtaagcaagtgtatttagtgggcttttggggggggggggcggggggagagggtggtggttttggggtggtcttgtttttgtttgtttttttttttttttgtt(miR19bprecursor)tgcagctctctgttagttttgcatagttgcactacaagaagaatgtagt(miR19a)tgtgcaaatctatgcaaaactgacggtggcctgctatttcctacatcgaggggtttgagtccttttttaaactcttttcttgccgccagtagcgaggtgtcggtccggcttctttctgatgacagctcctgtagcac(miR20)taaagtgcttatagtgcaggtagtgtttagttatctactgcattatgagcacttaaagtcctgctggctgtagagcttcaccttcagcgtgtctttcaatcaagcggtcttttaattgagcgctgt(miR19bprecursor)tctatggttagttttgcaggtttgcatccagctgtatggtactctgc(miR19b)tgtgcaaatccatgcaaaactgactgtggcagtgaagtccgttccaaagccaggaaaagtcaaacccct(miR92precursor)ttctacgcaggttgggatcagttgcaatgctttgtttgtctatgg(miR92)TATTGCACTTGTCCCGGCCTGTtgaggttgctggggatcgtggccgaaagatgttaaaatgtggcaggtaactcaacgagttaattctgtggttcagtgctctagggggacggggccatagccgaccctctggct
Oa2884 / 1 / new miR
miR 222 / ultra222
5162137-5162214 / cccctcaatCGCTCAGTAGTCAGTGTAGATcctgtattctaatcagcagctacatctggctactgggtctctgagggt
OA2909 / 1 / new miR
miR 490 / chr. 10
8756544-8756636 / agttcacggttcgacgCCATGGATCTCCAGGTGGGtcaaggttagagacgcaccaacctggaggactccatgctgtcgagctgatcacgagca
Oa2931 / 3 / miR 132 / no 100% match in Platypus-BLAST / TAACAGTCTACAGCCATGGTCGAT
Oa2940 / 1 / miR 7
new miR / ultra666
6525-6634 / agtggacgttggtctagttctgtgtggaagactagtgattttgttgtttgtagataactaaatcgaCAACAAATCGCAGTCTGCCATATggcacagaccatgcctctaca
Oa2971 / 1 / miR 23a / contig6812
2755-2837(+) / ccgccggcggctggggttcctggtgatgcgattttcttgtgctcgccaaaATCACATTGCCAGGGATTTCCAactggcggcgt
Oa2999 / 2 / miR-30c / contig1501
64389-64463 / acaggtaCTGTAAACATCCTACACTCTCAGCtgtagatgtgagaaagctgggagaaggctgtttactctctctgc
Oa3026 / 3 / miR 160
miR 124a / contig82070
3765-3875 / GgcgccgagatgggagactctgcctctcCGTGTTCACAGCGGACCTTGATTTAATGTCATACAATTAAGGCACGCGGTGAATGCCAAgagcggagccccggatcggcgcg
For table S23:
Column 1: Experimentally identified RNAs are indicated by Oa and a contig number.
Column 2: The number of sequence-clones presented in the cDNA library.
Column 3: Name of Y-RNA.
Column 4: Sanger genomic location.
Column 5: Y-RNA full-length sequences from the cDNA library.
Table S 23: Full-length Y cDNAs
name / numberof seq. clones / Y-RNA / Sanger
genomic location
/ sequenceOa1694 / 59 / Y5 / chr.4
8366163-8366253 / AGTTGGTCCGAGAGTTGTGGGTTATTGTTACATTTGATTCGAATGGAACATTGTCTCCCCCCACAACTGAGTTTGACTGGCTTTGCTTTTC
Oa1740 / 34 / Y1 / chr. 4
8382416-8382528 / CGGCTAGTCCGAAGGTAGTGAGTTATCACAATTGATTGTTCACAGTCAGTTACAGATCGATATCCCTGTTCTCTCTTCCTCCCACCTTCTCACTACCGTATTTGACTAGTCTT
Oa2078 / 7 / Y3 / chr.4
8374777-8374876 / GGCTGGTCCGAGTGCAGTGGAATTTATAATTAATTGATCACAGTCAGTTACAGATTTCTTTGTTCCTTCTCCACTCCCACTGCTTCACTTGACTAGTCTT
Oa2147 / 6 / Y4 / chr.4
8369863-8369954 / GGCTGGTCCGATAGTAGTGGGTGCCCAGAACTCTTTAATATAGTTTCACTAAACTTGGTATATTCCACCTCACTGCTAAACTTGACTGGCCT
For table S24:
Column 1: Experimentally identified 7SL RNAs are indicated by Oa and a contig number.
Column 2: The number of sequence-clones presented in the cDNA library.
Column 3: Name of RNA.
Column 4: Sanger genomic location.
Column 5: 7SL RNA full-length sequences. Capital letters represent sequences from the cDNA library. Lower case letters represent genomic BLAST sequences. cDNAs are extended to presumed full-length (compared to human 7SL).
Table S 24: Full-length 7SL cDNAs
name / numberof seq. clones / RNA / Sanger
genomic location
/ sequenceOa1911 / 4 / 7SL / chr. 4
32318640-32318939 / CGCCGGGCGCGGTGGCGCGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGCTCAGGAGTTCTGGGCTGCAGTCCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGTATCAATATGGTGATCCTCCGGGAGCGGAGGGTTACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCCGATCAGTAGTGGGATCGCGCCTGTGAATAGTTGCTGCAGTCTAGCCTGGGCAAGATAGTGAGACACAGTCTTT
Oa2517 / 1 / 7SL / chr.4
32323659-32323957 / gccgggcgcggtggcgcgcgcctgtagtcccagctactcgggaggctgaggctggaggatcgcttgagctcaggagttctgggctgcagtccgctatgccgatcgggtgtccgcactaagttcggtatcaatatggtgatcctccgggagcggagggttaccaggttgcctaaggaggggtgaaccggcccaggtcggaaacggagcaggtcaaaactcccgtgccgatcagtagtgggatcgcgcctgtgaatagtTGCTGCAGTCTAGCCTGGGCAAGATAGTGAGACACGGTCTCT
sequence: lower case: extrapolated cDNAs to presumed full-length (compared to human 7SL)
For table S25.1:
Column 1: Experimentally identified mitochondrial tRNAs are indicated by Oa and a contig number.
Column 2: The number of sequence-clones presented in the cDNA library.
Column 3: Name of tRNA.
Column 4: GenBank accession numbers.
Column 5: tRNA full-length sequences. Lower case letters represent genomic BLAST sequences. Capital letters represent sequences from the cDNA library. Lower case letters represent genomic BLAST sequences. cDNAs are extended to presumed full-length (compared to human). [CCA]: cDNA specific, not found by BLAST.
Table S 25.1: Annotated mitochondrial full-length tRNAs
name / numberof seq. clones / RNA / NCBI
genomic location / sequence
Oa1669 / 339 / tRNA-Val / X83427 Ornithorhynchus anatinus mitochondrial DNA
1016-1081 / CAAAATGTAGCTTAATCCAAAGCACTCAGCTTACACCTGAAAGATATTTTACATAAATCATTTTGA[CCA]
Oa1698 / 119 / tRNA Phe / X83427 Ornithorhynchus anatinus mitochondrial DNA
1-72 / GCACTCGTAGCTTAAACTCTTAAAGCAATACACTGAAAATGTTTAGATGATTCGTAACTGAACCCGAGCGCA[CCA]
Oa1704 / 15 / tRNA Ile / X83427 Ornithorhynchus anatinus mitochondrial DNA
3764-3832 / agaaatatgtCTGACAAAAGAGTTACATTGATAGCGTAAATTATAGAGGTTAAAATCCTCTTATTTCTA[CCA]
Oa1713 / 113 / tRNA Gly / X83427 Ornithorhynchus anatinus mitochondrial DNA
9457-9526 / ATTTCTCTAGTATCAATTAGTACAAGTGACTTCCAATCATTAAGTTTTGGTCTTAACCCAAAGAGAAATA[CCA]
Oa1714 / 70 / tRNA-Asp / X83427 Ornithorhynchus anatinus mitoch. DNA
7006-7073 / GAAGTGTTAGTTAATGAATAACATAGCCTTGTCATAGCTAAATTACAGACTACAATCTGTACACTTTA[CCA]
Oa1717a / 60 / tRNA Lys / X83427 Ornithorhynchus anatinus mitochondrial DNA
7762-7827 / CATAAAGAAGCTATTGCAGCAGTAACCTTTTAAGTTAAAGATTGAGATACACTTTCTCCTTTATGA[CCA]
Oa1732 / 153 / tRNA-Ser / X83427 Ornithorhynchus anatinus mitochondrial DNA
11678-11735 / CGAGAGAGAAATAAGAACTGCTAATCCTTAACTTCATGCCTAACCACATGACTCTactt
Oa1746 / 30 / tRNA Gln / X83427 Ornithorhynchus anatinus mitochondrial DNA
3901-3830 / TAGAGTATAGTGTAATTGAGAACACAGAAGTTTTTGAAGCTTGAGATGTAGGTTTAAATCCTATTATTCTAG[CCA]
Oa1780 / 35 / tRNA Leu / X83427 Ornithorhynchus anatinus mitochondrial DNA
2644-2718 / attAAGGTGACAGAGACCGGTAATTGTGTAAAACTTAAGCTTTTATAGTCAGAGGTTCAAATCCTCTCCTTAATA[CCA]
Oa1807 / 46 / tRNA Arg / X83427Ornithorhynchusanatinus mitochondrial DNA
9872-9940 / AGGTGATTAGTCTAGACAAGACTATTGATTTCGGCTCAATTAACCCTGGTATTAATCCATGATCACCTA[CCA]
Oa1903 / 33 / tRNA Trp /
X83427 Ornithorhynchus anatinus mitoch. DNA