File No: NA/635October 1999

NATIONALINDUSTRIALCHEMICALSNOTIFICATIONAND ASSESSMENTSCHEME

FULLPUBLICREPORT

2-cyclohexyl propanal

ThisAssessmenthasbeencompiledinaccordancewiththeprovisionsoftheIndustrialChemicals(NotificationandAssessment)Act1989(theAct)andRegulations.ThislegislationisanActoftheCommonwealthofAustralia.TheNationalIndustrialChemicalsNotificationandAssessmentScheme(NICNAS)isadministeredbytheNationalOccupationalHealthandSafetyCommissionwhichalsoconductstheoccupationalhealthsafetyassessment.TheassessmentofenvironmentalhazardisconductedbytheDepartmentoftheEnvironmentandtheassessmentofpublichealthisconducted bythe Department of Health and Aged Care.

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Director

Chemicals Notification and Assessment

FULL PUBLIC REPORT

27 October, 2000

NA/635Page 1 of 24

NA/635

FULLPUBLICREPORT

2-cyclohexyl propanal

1.APPLICANT

Kao(Australia)MarketingPtyLtdof103YerrickRoadLAKEMBANSW2195hassubmittedastandardnotificationstatementinsupportoftheirapplicationforanassessmentcertificate for 2-cyclohexylpropanal.

2.IDENTITY OFTHE CHEMICAL

ChemicalName:2-cyclohexyl propanal

ChemicalAbstracts Service

(CAS) Registry No.:2109-22-00

Other Names:PollenalII

Trade Name:PollenalII

Molecular Formula:StructuralFormula:

C9H16O

Molecular Weight:

140.2

MethodofDetection

and Determination:ultraviolet-visible (UV/Vis), infrared (IR), nuclear

magnetic resonance (NMR) and mass spectroscopy;gasliquid chromatography(GLC)

SpectralData:UV/Vis: the notified chemical in ethanol exhibited anabsorbance peak at 220 nm

IR: the 10 strongest peaks were 2 926, 2 853, 2 697,

1 725, 1 449, 1 399, 1 375, 1 001, 889 and 401 cm-1;

the peak at 2 926 was ascribed to CH, that at 2 697 toCH(C=O) and that at 1 725 to C=O

NMR:1H-NMR was provided

Comments on Chemical Identity

The notified chemical is a well defined simple aldehyde containingacyclohexylmoiety. Thenewchemicalalsocontains up to 4%of unidentifiedimpurities.

The notifier provided comprehensive spectroscopic data -IR, UV/Vis, NMR and massspectroscopy- on the new chemical which maybe used to identifythe material.AGLC alsoaccompanied the notification.

3.PHYSICALAND CHEMICALPROPERTIESAppearance at 20°C

and 101.3 kPa:clear colourless, non-viscous liquid

Boiling Point:196.5-200.0°C at 1025 mbar

Specific Gravity:0.915

Vapour Pressure:0.0817 0.0065 kPa at 25°C

Water Solubility:423 mg/Lat 20°C

Henry’s Law Constant:27.04 Pa/m3/mole - see notes below.

PartitionCo-efficient

(n-octanol/water):logPow= 2.95 at 20C - see notes below

Hydrolysis as a Function

ofpH:no significanthydrolysis at pH 4 and 7, some hydrolysisat pH 9- see notes below.

Adsorption/Desorption:logKoc= 2.49 byQSAR calculations – see notes below

DissociationConstant:no data provided - see notes below

Surface Tension:notsurface active - see notes below.

Fat Solubility:totallymiscible - see notesbelow.

FlashPoint:91°C (closed cup)

Flammability:not flammableAutoignitionTemperature:188°CExplosive Properties: not explosive

Comments on Physico-ChemicalProperties

Watersolubilitywasdeterminedbystirringanexcessofthetestsubstancewith100mLofdistilledwater at 30 oCfor 1, 2 and 3 days,equilibratingfor1dayat20C,thenseparating theaqueousandnonaqueouslayersbycentrifugation.Thecontentofthenewchemicalintheaqueousphasewasthendetermined bygaschromatography.Therewaslittledifferencebetweentheresultsforthosesolutionspreparedbystirringfor1,2or3dayspriortoequilibration(solubilityat20oCwasdeterminedas432,432and405mg/L,respectively),whichindicate that the reported solubilityis reliable.

TheHenry’sLawconstantwasdeterminedfromthemolecularweight,measuredvapourpressureandwatersolubilityusingtheequation-H=MW(g/mole)xVapourPressure(Pa)/Water solubility(g/L).

Thedegreeofhydrolysiswasdeterminedat50oCatpH4,7and9overa5daytestperiod.After2.4hoursthedegreeofhydrolysiswasrespectively0.7%,1.8%,and10.9%,andafter5days9.5%,10.5%and61.9%.AlthoughhydrolysisisslowatpH4and7,itissignificantlyhigheratpH9.However,theresultsindicateahalf-lifeofbetweenonedayandoneyearforthechemicalat25oCandpH9,andgreaterthanoneyearatpH4and7.Consequentlythecompoundisnotexpectedtoexhibitsignificanthydrolysisunderambientenvironmentalconditions.

Then-octanol/waterpartitioncoefficientwasdeterminedusingtheshakeflaskmethod,withanalysesofboththeaqueousandorganicphasesperformedbygaschromatography.ThedeterminedvalueofLogPowindicatesthenewchemicalhashighaffinityforhydrocarbon-likeenvironments.Massbalancecalculationsonthequantitiesofnewchemicalpartitionedintothen-octanolandwaterphasesgaverecoveriesinexcessof96.8%,whichindicatesthatthe method used was appropriate for this determination, and the overall result is reliable.

LogKocwascalculatedfrom the value ofLogPowusingtherelationshipLogKoc=0.81xLogPow+0.10.Thisrelationshipisappropriateforpredominantlyhydrophobiccompounds.ThevalueforLogKocof2.49indicatesthatthechemicalmaypartitionintotheorganiccomponentofsoilsandsediments,andbecomeassociatedwiththesematerials.However,this tendencymaybe reduced because of the highwater solubility.

Thecompoundcontainsnofunctionalitiescapableofreadilydissociatinginaqueousmedia.The notifier indicated that dissociation constant data were not applicable.

Thenewchemicaliscompletelymiscibleinfatat37C(experimentalreportsubmitted),whichisinaccordwiththepredominantlyhydrocarbonnatureofthematerialandthehighvalue for LogPow.

Thematerialismarginallysurfaceactive,withthesurfacetension(Method84/449/EEC(A5)ofanaqueoussolutioncontainingapproximately90%ofthetestsubstancesaturationof60.6mN/m at 20.5oC (water = 72.66 mN/m).

Calculationsbasedonthemolecularstructureusingthequantitativestructureactivityrelationships(QSAR)oftheUSEnvironmentProtectionAgencyASTERdatabase(reference

2)furnishedthefollowingestimatesforenvironmentallyrelevantphysico-chemicalparameters.Wherecomparisonwithdatasuppliedbythenotifierispossible,theagreementisreasonable.

ASTER DATA (all calculatedusing QSAR)

PropertyQSAR estimate

Boiling Point:185C

Vapour Pressure:0.728 mm of Hg(90.05 Pa)

Water Solubility:374 mg/L

Henry’sConstant:35.9 Pa/m3/mole

log Kow:2.73

log Koc:2.82

Hydrolytic degradation halflife:hydrolysis is unlikely

4.PURITY OFTHE CHEMICAL

Degree ofPurity:
Toxic or Hazardous / 98.6% (range: 95.0 – 99.9%)
Impurities:
Chemical Name / CAS No. / Weight %
2-cyclohexyl propionic acid / not provided / 1.0%
unknown / 4.0%

Additives/Adjuvants:

Chemical NameCAS No.Weight %

2,6-ditertiarybutyl4-hydroxytoluene128-37-0 0.05%

5.USE,VOLUMEAND FORMULATION

Thenotifiedchemicalisto be used as a fragrance enhancer in household, toiletryand

cosmetic products.Itwillbeimportedatarateof0.5tonnesinthefirstyearincreasingto1.7tonnes/year bythe year 2002.

Thenotifiedchemicalwillbeimportedinsealed,unbreakablelacqueredsteeldrumsof32or200 Lcapacitymixed with other ingredients.

Thenotifiedchemicalisblendedintoaformulatedperfume,whichisthenincorporatedintohousehold products at a level of approximately0.1%.

6.OCCUPATIONALEXPOSURE

Thenotifierstatesthatitwillneedonlyonewatersideworker,forklifttruckdriverandtruckdrivertohandleclosedcontainersofthenotifiedchemical.Duringtransportorstorageofdrumscontainingthenotifiedchemicaloccupationalexposuremayoccurintheeventofaccidental spillage.

Thechemical,inliquidform,iseithermanuallyorautomaticallychargedtoamixertobeblendedintoaformulatedperfumeataconcentrationof0.1–5%whichisthenautomaticallyfilledintocontainers.Thebatchsizeswerestatedtobe25kg,50kg,100kg,500kgor1tonne.Thenotifierstatesthatqualitycontroltesting(sampling,analysisandodourevaluation)takes2to3minutes/day/personoronepersonatotalof6to9minutes/day.Compounding(blending)whichiseitherautomatedormanual,involvesasingleworkerfor2to3minutes/day.Discharge(fillingofcontainers)isanautomatedprocesssaidtoinvolveoneworkerfor5minutesperday.Duringadditionofthenotifiedchemicaltothemixerforproductionoftheperfumeformulation,exposuretospillsispossible.Someexposuretotheformulationcouldoccurduringqualitycontroltesting.Thenotifierstatesthatcuffedbutylrubber gloves, goggles, plastic face shields, aprons and bootsarewornduringtheseprocesses.Thenotifieralsostatesthatthereshouldbelocalexhaustventilationinplaceandsplashprooffillingdevicestopreventcontactwiththenotifiedchemical.Themixingvesselsarestatedtoretain0.05%oftheformulation(maximum0.5kgperbatch)sothatexposuretosmallamounts of the chemical duringsolvent washingis possible.

Exposuretospillsispossibleduringadditionoftheperfumeformulationtootheringredientsfollowedbyblendingintohouseholdproductsanddispensingsuchproductsintocontainers.Localexhaustventilationisinplaceoverthemixingvessels.Theperfumeformulationisaddedtothemixingvesseleithermanuallyorautomaticallybyasingleworker.Fillingofcontainers,suchascartons,plasticbottles,plasticfilmisanautomaticormanualprocess.Thisisdoneatanumberofcustomersitesalthoughthedetailsarenotavailable.Theperfume in incorporated into household products at approximately0.1%.

7.PUBLIC EXPOSURE

Thenotifiedchemicalwillenterthepublicdomainashouseholdproduct(egdetergents,toiletries,cosmetics)containingthenotifiedchemicalatalowconcentration(approximately0.1%).Althoughthepublicwillmakedermalandinhalationalcontact,andpossiblyeyecontact(eg.whileusingshampooscontainingthenotifiedchemical)withthenotifiedchemical,exposureislikelytobenegligiblebecauseofthelowconcentrationofthenotified

chemicalintheproducts.Thepotentialforpublicexposuretothenotifiedchemicalduringtransport, reformulation and use or from disposal is assessed as negligible.

8.ENVIRONMENTALEXPOSURERelease

Thenewproductisusedtoprepareperfumeblends,whicharesubsequentlyincorporatedinto

soaps,detergents,fabricsoftenersandotherhouseholdproducts,andmaycontainbetween

0.04and0.23%ofthechemical.Thenotifierindicatedthattheseproductionactivitieswouldbeperformedbyanumberofdifferentcompanies.However,itisexpectedthatproductionwilltakeplaceinpurposeconstructedfacilities,andthenotifiermadethefollowingestimatesinrespectofreleasetotheenvironmentduringperfumeblendingandmanufactureofthefinalproducts.

Thenotifierindicatesthatduringblendingoftheperfumemixture,0.05%ofthe newchemicalislostthroughwashingoutthemixingvessels,andonanannualbasisthisamountstoamaximumlossof0.85kg.Itwasalsostatedthatmaterialreleasedintheformulatingplantsas a result of equipmentwashing(andpresumablyanyspillage) is sent with other wastetoon-sitetreatmentfacilitieswhichmayincludeunitoperationssuchasdissolvedairflotationandgranulatedcarbonfilters.Itisstatedinthesubmissionthat94%(annuallyaround0.80kg)ofthenewchemicalwouldberemovedfromthewastewaterbythistreatmentandbecomeincorporatedintothesolidwastestreamandincinerated.Thetreatedwastewater,containingtheremaining6%(annuallyaround50g)ofchemicalispresumablydischarged to the sewer systems.

Itwasindicatedinthesubmissionthatnoliquidwastestreamsareproducedduringproductionofthesoap,detergentandotherconsumerproductsintowhichtheperfumeblendisadded,butthataround0.01%ofthenewchemical(annually170g)maybelostasaconsequenceofsteamcleaningthemixingvesselsatproductchangeover.Presumablythiswouldalsobesenttothewatertreatmentplantwhere94%(annually160g)wouldbecomeincorporated in solid residuals and be incinerated.

Noreferencetothequantitiesofchemicallikelyto belost andreleasedas aresult ofaccidentalspillagewasmadeinthesubmission.However,thisassessmentestimatesthat1%oftotalimportquantitycouldbelostthroughaccident,whichamountstoanannualreleaseofaround 17 kg.If these spills arecleanedupwithwateranddivertedtowastewatertreatmentatthemanufacturingsitewhere94%ofthechemicalisremovedandincinerated,anestimated

1.0 kgof chemical could be released from the manufacturingsites to sewage.

Thenotifierstatedthattheemptysteeldrumsoftheimportedchemicalwouldbesentforrecycling.However, it is possible that the emptycontainerswouldbeplacedintolandfill,andalthoughnoestimatesoftheamountofresidualchemicalleftinthedrumswaspresentedintheapplication,thisassessmentestimatesthisat0.05%oftheimportquantity,oraround0.85kgper annum.

However,thenewchemicalisafragranceenhancerforuseindomesticcleaningproducts,andconsequentlyallwillbeeventuallyreleasedintotheenvironmentasaconsequenceof

normalproductusage.Itisexpectedthatthisreleasewouldbeprimarilytotheseweragesystem,althoughbecauseofthemeasuredvapourpressureof81.7Paat25oC,muchwouldbe expected to volatilise andenter the atmosphere.

Emptycontainersoftheconsumerproductsarelikelytocontainsomeresidualunusedproduct;thesepackageswouldbediscardedwithdomesticgarbageandbedisposedoftolandfill.

Fate

ThenotifierprovidedalaboratoryreportontheassessmentofthebiodegradationofthenotifiedchemicalconductedinaccordancewiththeOECDTestGuidelineTG301D(ClosedBottleTest).Theresultsofthistestindicated11%lossofinitialchemicaloxygendemand(COD)ofthetestmaterialafter28days,andaccordinglythenotifiedchemicalcannotbeclassed as readilybiodegradable.

Allthenewchemicalwilleventuallybereleasedintotheenvironment,andthemajoritycouldbeexpectedtobedischargedintoseweragesystems.However,oncereleasedinthismanner,the relativelyhighvapour pressure indicatesthatasignificantfractionwouldpartitionintotheatmosphere.TheSimpleTreatModel(EuropeanCommission1996Part2)maybeusedtoestimatepartitioningintodifferentcompartments,fortheproportionofchemicalwhichreaches the sewage treatment plant (ie is notvolatilisedorotherwisedestroyedduringpassagetotheplant).BasedonaHenry’sLawConstantof27.04Pa/m3/mole,aLogKowof2.95andthecompoundnotbeingbiodegradable,themodelindicatesthatthechemicalisexpectedtopartition into the air,water andsewer sludge compartmentsasfollows -

AIRWATERSEWERPLANT

SLUDGE 44%50% 6%

MackayLevel1calculationsfromtheASTERdatabase(USEnvironmetalProtectionAgency1998)indicatethatwhenreleasedtotheenvironmentthechemicalwouldpartitionintothevarious compartments as listed below –

Atmospheric compartment92.08%

Water compartment7.31%

Soil compartment0.31% Aquatic biota compartment0%

TheMackaymodelassumesanequilibriumisestablishedbetweenallphases.Intheenvironmentanequilibriumstatewillnotbereachedaschemicalwhichreachestheatmospherewillbeeffectivelyremovedfromthesystem(bydiffusionintotheatmosphereorblownawaybywind).Consideringtheassumptionsandapproximationsinherentinboththesemodels,particularlyinrespectofthesignificantlydifferentHenry’sLawConstantandpartitioncoefficientusedineachmodel,thedifferencebetweenthetwosetsofresultsisnotsurprising. Both methodologies indicate significant partitioningto the atmosphere.

Oncereleasedtotheatmosphereitisconsideredthatthechemicalwouldbequicklydecomposedthroughphotolyticallypromotedfreeradicalreactions.Hence,overtimethesediment/water andwater/airpartitioning willbedriventowardthelossofthechemicaltotheatmosphere.Intheatmosphereitislikelythatthesubstancewillberapidlydegradedthroughreactionwithhydroxylradicals(throughhydrogenabstractionmechanisms).AcalculationbasedonthemethodsdescribedinOECDEnvironmentalMonographsNo61indicatesthatinthetropospherethenewchemicalwouldreactwithinthismanner,witharateconstantestimatedas22.74x10-12cm3/molecule/sec.Rateconstantsofthisorderareindicativeoffastdegradation(OECDEnvironmentalMonographNo61),andthecompoundisnotexpected to persist in the atmosphere.

ThenewchemicalishydrophobicincharacterwithLogPow=2.95,andestimatedLogKoc=

2.49.Consequentlywhenreleasedintothesewersystemsomewouldbeexpected toassociatewiththeorganiccomponentoftheparticulatematterpresentintherawsewage,andeventuallybecomeincorporatedintosediments.Hereitwouldbeslowlydegradedthroughbiological and abiotic processes to water, carbon dioxide and methane.

Theresidualchemical,whichisdisposedoftolandfillwithinemptydrums,discardedconsumerpackagingorwithresidualsolidsderivedfromwatertreatmentattheproductionfacilities,wouldalsobeexpectedtovolatiliseandentertheatmosphere.However,somechemicalmayremainadsorbedtosoilparticles,andinthissituationwouldbeexpectedtobeslowlydestroyedbysimilarmechanismstothoseoperatinginsediments.Any wastematerialcontainingthenotifiedchemicalplacedintocompostfacilitiescouldalsobeexpectedtobedestroyedthrough aerobic and anaerobicbiologicaldegradationprocesses.Incinerationofthematerial would produce water vapour and oxides of carbon.

TheASTERcalculationsmentionedabovealsoestimateabioaccumulationfactorof58forthecompoundinfish(fatheadminnow).Thisisalowvalueforthisparameterindicatinglittlepotentialforbioaccumulation.Althoughthenewchemicalishydrophobic,itisvolatileandisconsequentlynotexpectedtohaveprolongedresidencetimesintheaquaticcompartmentor to bioaccumulate.

9.EVALUATION OFTOXICOLOGICALDATA

9.1Acute Toxicity

Summary ofthe acute toxicity of2-cyclohexyl propanal

TestSpeciesOutcomeReference

acute oral toxicityratLD505 000 mg/kgfor

males

LD502 000 mg/kgforfemales

(Allan, 1992a)

acute dermal toxicityratLD502 000 mg/kg(Allan, 1992b)

acute inhalationtoxicity

ratLC50 5.32 mg/L(Jackson, 1994)

skin irritationrabbitslight to moderate irritant(Liggett, 1992a)

eye irritationrabbitslight irritant(Liggett, 1992b)

skin sensitisationguinea pigsensitiser(Parcell, 1992)

9.1.1Oral Toxicity (Allan, 1992a)

Species/strain:rat/Sprague-Dawley

Number/sex of animals:5/sex(group 1)

5 females (group 2)

Dose:

5 000 mg/kg(group 1)

2 000 mg/kg(group 2)

Observation period:14 days

Method of administration:gavage

Clinical observations:piloerectionwasobservedinallratsandincreased

salivationinratstreatedwith5000mg/kgwithinfiveminutesofdosing;afterday1abnormalgaitwasobservedinallrats;hunchedposture,lethargy,decreasedrespiratoryrate,ptosisandpalloroftheextermitieswereobservedinratsdosedwith5000mg/kg;prostrationwasobservedinonedeadfemale;recoveryofrats,asjudgedbyexternalappearanceandbehaviour,wascomplete:byday2forratsdosedwith2000mg/kg;byday3forallbut one rat dosed at5000mg/kg;andday4foronefemaledosedat5000mg/kg;bodyweightgainwas not affected bytreatment

Mortality:2 females from group1

Morphological findings:none

Test method:Directive84/449/EEC(OJNo.L251)PartBMethodB.1(EuropeanEconomicCommunity,1984)

LD50:5 000 mg/kgfor males

2 000 mg/kgfor females

Result:thenotifiedchemicalwasofverylowacuteoraltoxicityin rats

9.1.2Dermal Toxicity (Allan, 1992b)

Species/strain:rat/Sprague-Dawley

Number/sex of animals:5/sex

Observation period:14 days

Method of administration:thenotifiedchemical,aviscousliquid,wasspread

evenlyoveranareaofapproximately25cm2andcovered with an occlusive dressingfor 24 hours

Clinical observations:nosigns of systemic reaction to treatment; well

definederythemainninerats,slighterythemainoneratandslightoedemainmaleratswereobserved on day2; all animals recovered byday5

Mortality:none

Morphological findings:none

Test method:Directive84/449/EEC(OJNo.L251)PartBMethodB.3(EuropeanEconomicCommunity,1984)

LD50:2 000 mg/kg

Result:the notified chemical was of low acute dermaltoxicityin rats

9.1.3Inhalation Toxicity (Jackson, 1994)

Species/strain:rat/Sprague-DawleyNumber/sex of animals:5/sex(test and control groups)Observation period: 14 days

Dose(Exposure Concentration)5.32 mg/L

Method of administration:snout onlyexposure to (liquid) aerosol (87%within

respirable range) for 4 hours

Clinical observations:asoiledappearanceofthefurwasnotedonday0

fortestandcontrolanimals;exaggeratedrespiratorymovements,staggeringgaitandpoorgroomingnoteduptoday3;brownstainingaroundthesnout,jawsandaroundtheeyeswerepersistentafterday3;foodconsumptionofthetestgroupslightlyreduced on day1

Mortality:none

Morphological findings:
Test method: / none
OECDguidelineTG403(Organisationfor
EconomicCo-operationandDevelopment,1995-
1996)
LC50: / > 5.32 mg/L
Result: / the notified chemical was of very low acuteinhalation toxicityin rats
9.1.4 / Skin Irritation (Liggett, 1992a)
Species/strain: / rabbit/New Zealand White
Number/sex of animals: / 3/females
Observation period: / 12 days
Method of administration: / 0.5mLofthenotifiedchemicalappliedundera

625mm2 gauzepadunderocclusivedressingfor4 hours

Draize scores:(Draize, 1959)of unirrigated eyes

Timeaftertreatment(days)

Animal#

Erythema

*123456789

111222221a0a0a

21122222a1a0a0a

31122222a1a1a0a

Oedema

11111111100

21122221100

31122221110

seeAttachment1forDraizescalesa=sloughingofepidermis *=approximately30minutesafterremovalofthedressing

Test method:Directive84/449/EEC(OJNo.L251)PartBMethodB.4(EuropeanEconomicCommunity,1984)

Result:veryslighterythemawasobservedinallthreeanimals30minutesafterremovalofthedressing;well-definederythema wasseen in allthree animalsfromday2to6andcontinuedasveryslighterythemauptoday7andinoneanimaluptoday

8;veryslightoedemawasobservedinallthreeanimals30minutesafterremovalofthedressingwhichpersisteduptoday7inoneanimal;slightoedemawasobservedintwoanimalsfromday2to5andcontinuedasveryslightoedemauptoday7andday8(oneanimal);desquamationofthestratumcorneum(sloughing)wasseenin2animalsfromday6andinallthreeanimalsfromday7today10

thenotifiedchemicalwasamoderateskinirritantin rabbits

9.1.5Eye Irritation (Liggett, 1992b)

Species/strain:rabbit/New Zealand WhiteNumber/sex of animals: one male and two femalesObservation period: 7days

Method of administration:0.1 mL of the notified chemical into the

conjunctival sac of one eye

Test method:Directive84/449/EEC(OJNo.L251)PartBMethodB.5(EuropeanEconomicCommunity,1984)

Result:dullingofthecorneawasobservedin2animalsonehourafterinstillation;scatteredanddiffuseareasofcornealopacitywasobservedin2animalsonday1andpersisteduptoday3inoneanimal;noiridaleffectswereobserved;allrabbitsexhibitedmildtomoderaterednessandchemosisoftheconjunctiva1hrpost-instillationwhichpersistedtoday1or2;noothereffectswereseenup to 7 dayspost-instillation

the notified chemical was slight eye irritant inrabbits

9.1.6Skin Sensitisation(Parcell, 1992)

Species/strain:guinea pig/Dunkin-Hartley

Number of animals:20 test; 10 controls

Induction procedure:3pairsofintradermalinjectionsinthescapularregion as follows:

-Freund’s Complete Adjuvant (FCA)diluted 1:1 with water;

-notifiedchemical,10%(v/v)inAlembicol D

-notified chemical, 10% (v/v) in FCAand Alembicol D 1:1

sevendaysaftertheabovetreatment,topicalinductionwasperformedbyapplyinga8cm2filterpapersoakedwith0.4mLofthenotifiedchemicaltothesamescapularregionunderocclusivedressingfor48hours;controlanimalsweresimilarlytreated but without the notified chemical

Challenge procedure:14 days after topical induction, test and control

animalswerechallengedusingnotifiedchemical,at 75% and 40% (v/v) in Alembicol D

anteriorsiteontheflankofeachtestanimalwastreatedwith0.2mLofthenotifiedchemical,75%(v/v)inAlembicolDandtheposteriorsitewastreatedwith0.2mLofthenotifiedchemical,40%(v/v)solutioninAlembicolDunderocclusivedressingfor24hours;controlanimalsweresimilarlytreated but without the notified chemical

Challenge outcome:

Challenge

Test animalsControl animals

concentration24 hours*48 hours*24 hours48 hours

75% (v/v) inAlembicol

40% (v/v) inAlembicol

**20/2020/200/100/10

20/2019/200/100/10

* timeafterpatchremoval

**numberofanimalsexhibitingpositiveresponse

Challenge outcome:marked persistent erythema and oedema were

observedinalltestanimalonthechallengesitesat24 and 48 hours after patch removal

Test method:Directive84/449/EEC(OJNo.L251)PartBMethodB.6(EuropeanEconomicCommunity,1984)

Result:the notified chemical was a skin sensitiser in

guinea pigs

9.2Repeated Dose Toxicity (Edwards, 1992)

Species/strain:rat/Sprague-DawleyCrl:CD(SD)

Number/sex of animals:5/sex/group

Method of administration:thenotifiedchemicalincornoilwasadministered

bygavage

Dose/Study duration::control:0mg/kg/dayfor 28 days

low dose: / 15 /  / 
mid dose: / 150 /  / 
highdose: / 1 000 /  / 

Water consumption:a relative increase in water consumption was

observedinhighdosegroupmales(12.6%)andfemales(24.5%);atlowerdosegroupsthiseffectwas not remarkable

Clinical observations:occasional salivation (with greater frequency in

males)inmiddoserats;salivationinhighdoseratswaspersistentfromday2;waddlinggaitwasobservedinhighdoseratsaccompaniedbyaslightlethargy;occasionalpilo-erectionandthinappearance observed in highdose females

Clinical

chemistry/Haematologyclinicalchemistry:statisticallysignificantincreases

inglobulinlevelsaccompaniedbyanincreaseintotalproteinlevelsinmales;adecreaseinglutamicoxaloacetictransaminase(GOT)inhigh dosegroup;relativeincreaseinglucoseinhighdosefemales;alltheabovechangesincomparisontohistoricalrangewasconsideredtobeincidental;athighdoseadecreaseinchloride(p<0.01)inbothmaleandfemales,whichinfemales,wasaccompaniedbyincreasedphosphorousions;two(2/5)individualchlorideionvaluesamongfemalesandone(1/5)amongmaleswereslightlybelowthebackgroundrangeandmayindicatetreatmentrelatedeffects

haematology:statisticallysignificantincreaseinthrombotesttimeandvariationinmean cellvolumewereobservedinhighdosemalesbutwerenotdosedependent;decreasedeosinophillevelsobservedinhighdosefemaleswerewithinthehistorical range

Body/Organ weights:statisticallysignificantreductioninmean

bodyweightgaininhighdosemales;infemalesastatisticallyincrease was observed atweek 2only;

significantlylowerabsolutespleenweightsobservedinhighdosemalesandsignificantlyincreasedabsoluteandrelativekidneyweightsobservedinhighdosefemales;itwasarguedthatthemajorityofindividualvalueswerewithinthehistorical backgroundrange

Macroscopic findings:smallprostatesandminimalcontentsin seminal

vesicles in highdose males

Histopathology:focaltubularbasophiliaand/orcorticalscarringofthekidneyisthehighdosegroupwereconsideredbackground events

Test method:Directive84/449/EEC(OJNo.L251)PartBMethodB.7(EuropeanEconomicCommunity,1984)

Result:thetargetorganwasidentifiedastheblood,withchangesjudgedtobeofminorimportanceandadaptiveinnature;thenoobservableeffectlevel(NOEL)wasjudgedtobe15 mg/kg/dayonthebasis of salivation in rats at mid and highdose.

9.3Genotoxicity

9.3.1Salmonella typhimuriumReverse Mutation Assay (Jones Kitching, 1992)Strains: TA 1535, TA 1537, TA 1538, TA 98 and TA 100Concentration range: 0, 1.5, 5, 15, 50 and 150 g/plate

Test method:OECD guideline TG 471 (Organisation for

EconomicCo-operationandDevelopment,1995-1996)

Result:toxicityofthenotifiedchemicalwasnotedat500and5000g/platesothetopdoseinthemaintestwasreducedto150g/plate;noincreaseinthenumberofprototrophic(histidine-independent)backmutantswasobserveduptothetopdoseinthepresenceorabsenceofmetabolicactivationprovidedbyAroclor-inducedratlivermicrosomalpreparations(S9fraction);negativeandpositivecontrols gave the expected responses.

9.3.2Chromosomal Aberrations inHumanLympocytes in vitro(Jones et al., 1992)

Cells:lymphocytesfromhealthyhumanmaledonors,stimulatedto dividewith phytohaemagglutinin(PHA)

Treatmentregime:Aroclor1254-inducedratlivermicrosomal

preparations(S9fraction)wereaddedtoculturestreated for 16 hours with 0, 78.1, 321.5 and

425 g/mL orfor24hourswith0,19.5,312.5,625and5000g/mLofthenotifiedchemical;cultureswithout S9 fraction were treated at 0, 39.1, 156.3or

312.5 g/mLfor16hours;100cellswereexamined per dose level

Test method:OECDguidelineTG473(OrganisationforEconomicCo-operationandDevelopment,1995-1996)

Result:noincreaseinthenumberofchromosomalaberrations(eitherincludingorexcludinggaps)occurredinculturestreatedfor16hours(withorwithoutS9fraction)withthenotifiedchemicalrelativetocontrols;therewasastatisticallysignificantand reproducable increase in thenumberchromosomalaberrationsinculturestreatedat625

µg/mlfor24hoursinthepresenceofS9mix;inarepeattestof625µg/mlto5000µg/ml,therewasastatisticallysignificantincreaseinaberrantcellsatalldoses;positiveandnegativecontrolsgavetheexpected responses

thenotifiedchemicalwasclastogenicinhumanlymphocytes in the presence of S9 mix

9.3.3Micronucleus Assay in the Bone MarrowCells ofthe Mouse (Proudlock, 1992)

Species/strain:mice/CD-1

Number and sex of animals: 40/sex; 3groups

Doses:1920mg/kg;mitomycinC(12mg/kg)aspositivecontrolandaqueous1%methylcelluloseasnegative control

Method of administration:intragastric gavage;

Test method:similartoOECDguidelines474andEECannexVcommittee No. L251B

Result:noreductionintheratioofpolychromaticerythrocytesorsignificantincreaseinpolychromaticerythrocyteswereobservedatanydoselevelwhenobservedat24,48and72hourtimepoints

thenotifiedchemicalhasnotshownanyevidenceof causingchromosome damage

9.4Overall Assessment ofToxicological Data

Thenotifiedchemicalwasofverylowacuteoraltoxicity(males)andinhalationtoxicity(LD505 000mg/kgfororaltoxicity;LC505.32mg/Lforinhalationtoxicity)andoflowacuteoraltoxicity(females)anddermaltoxicity(LD502000mg/kg)inrats.Itwasaslighttomoderateskinirritantandaslighteyeirritantinrabbits. Itwas askin sensitiser in guinea pigs.

Clinical signs of persistent salivationwereobservedina28-dayoralrepeatdosestudyin rats at mid and highdoses.The NOELwas 15 mg/kg/day.

ThenotifiedchemicalwasclastogenicinhumanlymphocytesinthepresenceofS-9mixbutwasnotmutagenicinbacteriainvitroorinvivointhemousemicronucleusassay.Basedonthesetestresultsthenotifiedchemicalisconsideredtobeweaklygenotoxic.

Thenotifiedchemicalwouldbedeterminedtobeahazardoussubstanceaccordingtothe NOHSCApproved Criteria forClassifyingHazardousSubstancesintermsofskinsensitisationandpersistentskinirritation(NationalOccupationalHealthandSafetyCommission, 1994a).

10.ASSESSMENT OFENVIRONMENTALEFFECTS

Thenotifierprovidedthefollowingecotoxicitydatainsupportoftheirapplication.Theecotoxicitytests were performed in accordance withOECD TestGuidelines.

TestSpeciesResults(Measured)

AcuteToxicity[OECD 203]

Oncorhynchus mykiss

(Rainbow trout)

LC50(96 h) = 3.2 mg/LNOEC (96 h) = 1.5 mg/L

AcuteImmobilisation[OECD 202 Part 1]

Daphnia magnaEC50(48 h) = 1.2 mg/LNOEC(48 h) = 0.6 mg/L

Thetestsonrainbowtroutwereperformedusingsolutionsofthetestmaterialmadeupindechlorinatedwater.Stocksolutionsofthetestwereautomaticallydispensedintothe20Ltestvesselsatarateof0.35mL/h,whilethemediumitselfwascontinuouslyrenewedatapproximately118mL/min.Thetestswereconductedovera96hourperiodatacontrolledtemperatureof14oC.Fivesolutionsofthechemicalwithmeanmeasuredconcentrationsof0.8,1.5,2.8,4.8and9.0mg/Lweretested,togetherwithonecontrol.Solutionanalysiswasconductedbyextractionwithdichloromethanefollowedbygaschromatographicdeterminationoftheextractedtestchemicalonwatersamplestakenat0,24and96hoursaftercommencementofthetests.Themeasuredresultswere(withoneexception)alwayswithin 25% of the nominal concentrations.

Tenfishweretestedateachconcentration,andduringtheseteststhepHofthetestsolutionswasalwaysbetween7.1and7.2,whiledissolvedoxygenconcentrations(DOC)werealwaysbetween 10.1 and 10.3 mg/L.

Thetestsresultsindicatethatthenotifiedchemicalismoderatelytoxictotherainbowtroutwith a 96 hourLC50of3.2mg/LdeterminedusingthemethodofThompsonandWeil(1952).TheresponseslistedintherawdataweresuchthatProbitanalysiswasnotpossible,butthisassessment places the 96 hour LC50 between 2.8 and 4.8 mg/L.

Theacuteimmobilisationtestsondaphniawereperformedusingsolutionsofthetestmaterialmadeupindechlorinatedwater.Astocksolutionofthetestmaterial(containingTween80detergentandacetonetoassistsolubility)wasseriallydilutedandusedinastaticnonrenewalsystemovera48hourperiodatacontrolledtemperatureof21oC.Ninesolutionsofthechemicalwithnominalconcentrationsof0.32,0.58,1.0,1.8,3.2,5.8,10,18and32mg/Lweretested,togetherwithonecontrol.Solutionanalysiswasconductedonmediumsamplestaken at 0 and 48 hours, byextraction with dichloromethane followedbygaschromatographicdeterminationoftheextractedtestchemical.Therelevantmeasuredconcentrationswere0.6,2.1, 4.4 and 7.6 mg/L.

Tendaphniaweretestedinduplicateateachconcentration.DuringtheseteststhepHofthetestsolutionswasalwaysbetween7.1and7.2,whileDOClevels(measuredforthecontrolonly)werealwaysbetween8.0and8.9mg/L.Thecriterionfordecidingonimmobilisationwasiftheanimalswereunabletoswimaftergentleagitationofthetestvessel.Thetestsresultsindicatethatthenotifiedchemicalismoderatelytoxictodaphniawitha48hourEC50of 1.2 mg/Ldetermined usingthe method of Thompson and Weil (1952). Theresponseslistedin the raw data were such that Probit analysis wasnotpossible,butthisassessmentplacesthe48 hour EC50between 0.6 and 2.1 mg/L.

Thenotifierdidnotprovidelaboratoryreportsontheeffectofthechemicalondaphniareproductionoralgalgrowth,sincethesestudieshadnotbeenconducted.However,thisdeficiency was acknowledged and some calculated estimates comparing the Predicted

EnvironmentalConcentration(worstcasescenario)withPredictedNoEffectConcentration(taken as 1/1000 of the daphnia EC50) were presented.

TheQASRcalculationsoftheASTERdatabase(USEnvironmetalProtectionAgency1998)also furnished a predicted LC50= 8.7 mg/Lfor acute toxicityto Fathead minnow.

11.ASSESSMENT OFENVIRONMENTALHAZARD

Themajorityofthenewchemicalisaningredientofdomesticcleaningproductsandmostofthematerialwouldeventuallybereleasedintodomesticseweragesystemsasaconsequenceofproductuse.However,duetothevolatilityofthematerial,ahighproportionisexpectedtoenter the atmosphere.

Theecotoxicitydataindicatesthatthenewchemicalismoderatelytoxictotheaquatictestspecies.However,basedonmaximumestimatedannualimportsof1.7tonne,allofwhichiseventuallyreleasedtosewer,thedailyreleaseonanationwidebasisis8.2kg/day. Assuminganationalpopulationof18,000,000andthateachpersoncontributesanaverage150L/daytooverallsewageflows,thepredictedconcentrationinsewageeffluentonanationwidebasisisestimatedas1.7g/L.Whenreleasedtoreceivingwaterstheconcentrationisgenerallyunderstoodtobereducedbyafurtherfactorofatleast10,sothePredictedEnvironmentalConcentrationisaround0.17g/L.Thisisnearlyfourordersofmagnitudelessthanthedemonstratedchronictoxicitytothedaphnia(EC50=1.2mg/L),themostsensitivespeciesagainstwhich the new chemicalwas tested.

ThechemicalishydrophobicwithLogPow=2.95,indicatingsignificantaffinityfortheorganiccomponentofsoilsandsediments.TheSimpleTreatandMackayLevel1calculationsmentionedabovealsoindicatethatduetotherelativelyhighvapourpressuremuchofthechemicalwouldpartitionintotheatmosphereandbedestroyedby reactionswithhydroxyfreeradicals.Nevertheless,itislikelythatsomeofthechemicalwouldbecomeboundtosoilsandsediments,andhereisexpectedtobeslowlydegradedtowater,carbondioxideandmethanethroughbiologicalprocesses.Thesemechanismswouldoperatetocontinuouslyremovethechemicalfromtheaqueouscompartment,sooverallenvironmentalconcentrations would be unlikelyto increase with prolonged release of the chemical.

The above considerations indicate minimal hazardtotheenvironmentwhenthenewchemicalisusedasacomponentofdomesticproductsinthemannerindicatedbythenotifier.However,itshouldbeappreciatedthatthenewcompoundwillbeusedinperfumeformulationswithtwosimilarchemicals(NICNASsubmissionsNA/633andNA/634),whichwillbepresentintheproductsatsimilarlevels.Consequentlythesafetymarginforenvironmentalhazardwillbereducedbyafactorofapproximately10.AlgaeandchronicdaphniatestsareonlyavailableforthechemicaladdressedinthereportonNA/634,wherethechronictestshowsthatchemicalNA/634islesstoxicthantheacuteresultforeitherthepresent chemical or NA/633.

12.ASSESSMENTOFPUBLICANDOCCUPATIONALHEALTHANDSAFETYEFFECTS

Onthebasisofthesubmittedtoxicologicaldatathenotifiedchemicalisunlikelytoexhibitacuteorsubacutesystemictoxicity.However,itmaybeaslighttomoderateskinirritantandslighteyeirritant.Itisastrongskinsensitiserandisweaklygenotoxic.ThenotifiedchemicalwouldbeclassifiedashazardousaccordingtotheNOHSCApprovedCriteriaforClassifyingHazardousSubstancesintermsofskinsensitisationandpersistentskinirritation.ItwouldwarranttheriskphrasesR38IrritatingtoskinandR43Maycausesensitisationbyskin contact.The number and type of genotoxic studies conducted are insufficienttoconsidera hazardous substances classification for genetic effects.

Theriskofadversehealtheffectstoworkersinvolvedintransportandstorageisconsideredto be minimal except in thecaseofaccidentalspillage,wheretheremaybeaslightriskofeyeorskinirritationandastrongchanceofskinsensitisationifexposureisrepeated.Thereisariskofeyeorskinirritancyorskinsensitisationduringmanualadditionofthenotifiedchemical to vessels used for formulatingperfume for household products, duringsamplingforqualitycontrolpurposesandduringsystemmaintenance.Theriskoftheseeffectsislikely tobehighestduringhandlingoftheundilutedchemicalforexamplewhenaddingtomixingvessels.Theriskofirritationismuchreduced,buttherealpossibilityofskinsensitisation(andrespiratorysensitisation)remainsforworkershandlingtheformulatedperfume.Thenotifierstatesthattheconcentrationofthechemicalintheformulatedperfumeisupto5%,whichwouldbeahazardoussubstancebasedonskinsensitisation,wherethecutoffconcentrationis1%.Theriskofeyeorskinirritationduringautomaticpackagingislikelytobenegligiblehowever,giventhatthematerialisastrongsensitisersensitisingreactionsmaydevelopiftheexposuretothechemicaloccurs.Thenotifierstatesthatworkerswilluseorganicsolventresistantgloves,suchasbutylrubbergloves,andsafetyglasses;processeswilloccurunderlocalexhaustventilation.Itiscriticalthatallfeasiblestepsaretakentoreduce dermal and inhalation exposure to the notified chemical.

Thereisaslightriskofeyeorskinirritationandarealriskofsensitisationforworkersinvolvedinmanualadditionoftheperfumeformulationtovesselsusedtoblendhouseholdproducts.Oncetheenduseproductsaremixed,theriskofirritationandsensitisationresultingfromexposuretothenotifiedchemicalisverylowasitispresentinverysmallamounts (0.1%).

Workersconductingqualitycontroltesting,machinemaintenanceandpackagefillingwillbeatan equivalentrisk and need to take precautions to avoid contamination with the chemical.

Given thestrongsensitisingeffectsseeninexperimentalanimals,thisassessmentrecommendsthatworkerswhohavebecomesensitisedshouldnotcontinuetohandlethechemical in the workplace.

Althoughmembersofthepublicwillmakedermalandinhalationalcontactandpossibleeyecontactwiththenotifiedchemical,exposureislikelytobenegligiblebecauseofthelowconcentration of the notified chemicalinconsumerproducts(approximately0.1%).Althoughthenotifiedchemicalisaslighttomoderateskinirritant,aslighteyeirritant,askinsensitiserandisweaklygenotoxic,thesehazardsareunlikelytobesignificantbecauseofthelowconcentration of the notified chemical in the products.

13.MATERIALSAFETY DATA SHEET

TheMSDSforthenotifiedchemicalwasprovidedinaccordancewiththeNationalCodeofPracticeforthePreparationofMaterialSafetyDataSheets(NationalOccupationalHealthand SafetyCommission, 1994b).

ThisMSDSwasprovidedbytheapplicantaspartofthenotificationstatement.Itisreproducedhereasamatterofpublicrecord.Theaccuracyofthisinformationremainstheresponsibilityof the applicant.

14.RECOMMENDATIONS

Tominimiseoccupationalexposuretothenotifiedchemicalthefollowingguidelinesandprecautions should be observed:

  • SafetygogglesshouldbeselectedandfittedinaccordancewithAustralianStandard(AS)1336(StandardsAustralia,1994)tocomplywithAustralian/NewZealandStandard (AS/NZS) 1337 (Standards Australia/Standards New Zealand, 1992);
  • IndustrialclothingshouldconformtothespecificationsdetailedinAS2919(Standards Australia, 1987);
  • ImpermeableglovesshouldconformtoAS/NZS2161.2(StandardsAustralia/Standards New Zealand, 1998);
  • AlloccupationalfootwearshouldconformtoAS/NZS2210(StandardsAustralia/Standards New Zealand, 1994);
  • Spillageofthenotifiedchemicalshouldbeavoided.Spillageshouldbecleaneduppromptlywith absorbents which should then be put into containers for disposal;
  • Good personal hygiene should be practised to minimise the potential for ingestion;
  • A copyof the MSDSshould be easilyaccessible to employees.
  • Sensitised workers should not continue to handle the chemical in the workplace.
  • ThenotifiedchemicalmayberecommendedtotheNationalOccupationalHealthandSafetyCommissionforconsiderationforinclusionintheNOHSCListofDesignatedHazardous Substances.

15.REQUIREMENTSFOR SECONDARY NOTIFICATION

UndertheAct,secondarynotificationofthenotifiedchemicalshallberequiredifanyofthecircumstancesstipulatedundersubsection64(2)oftheActarise.Also,ifannualimportsofthenewmaterialexceed7tonnes(fourtimesthepresentestimates),wewillrequirefulltestresultsandreportsontheeffectsofthechemicalondaphniareproductionandtheinhibitionof algalgrowth.

16.REFERENCES

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AllanSA(1992)PollenalII:AcuteDermalToxicitytotheRat,ProjectNo.91944D/KSP176/AC, Huntingdon Research Centre Ltd,Cambridgeshire,England.

EdwardsJA (1992)PollenalII:Twenty-EightDayOralToxicityStudyintheRat,ProjectNo.KSP 181/920454, Huntingdon Research Centre Ltd,Cambridgeshire,England.

EuropeanCommission(1996)TechnicalGuidanceDocumentinSupportofCommissionDirective93/67/EEConRiskAssessmentforNewNotifiedSubstancesandCommissionRegulation (EC) No 1488/94 on Risk Assessment for ExistingSubstances - PARTIII.

EuropeanCommission(1996)TechnicalGuidanceDocumentinSupportofCommissionDirective93/67/EEConRiskAssessmentforNewNotifiedSubstancesandCommissionRegulation (EC) No 1488/94 on Risk Assessment for ExistingSubstances - PART II.

JonesE,AkhurstLC,HowellA,etal.(1992)PollenalII:MetaphaseChromosomeAnalysisofHumanLymphocytesCulturedinvitro,ProjectNo.KSP183/920370,HuntingdonResearch Centre Ltd,Cambridgeshire,England.

JonesEetal(1992)PollenalII:BacterialMutationAssay,ProjectNo.KSP182/911593,Huntingdon Research Centre Ltd,Cambridgeshire,England.

LiggettMP(1992)PollenalII:SkinIrritationtotheRabbit,ProjectNo.911010D/KSP177/SE, Huntingdon Research Centre Ltd,Cambridgeshire,England.

LiggettMP(1992)PollenalII:EyeIrritationtotheRabbit,ProjectNo.911009D/KSP178/SE, Huntingdon Research Centre Ltd,Cambridgeshire,England.

NationalOccupationalHealthandSafetyCommission(1994a)ApprovedCriteriaforClassifyingHazardousSubstances[NOHSC:1008(1994)].AustralianGovernmentPublishingService, Canberra.

NationalOccupationalHealthandSafetyCommission(1994b)NationalCodeofPracticeforthePreparationofMaterialSafetyDataSheets[NOHSC:2011(1994)].AustralianGovernment PublishingService, Canberra.

OrganisationforEconomicCo-operationandDevelopment(1992)OECDEnvironmentalMonographsNo.61,"TheRateofPhotochemicalTransformationofGaseousOrganicCompounds in Air Under Tropospheric Conditions",OECD,Paris, France.

ParcellBI(1992)PollenalII:SkinSensitisationintheGuineaPig,ProjectNo.920037D/KSP 179/SS, Huntingdon Research Centre Ltd,Cambridgeshire,England.

StandardsAustralia(1987)AustralianStandard2919-1987,IndustrialClothing.StandardsAssociation of Australia, Sydney.

StandardsAustralia(1994)AustralianStandard1336-1994,EyeprotectionintheIndustrialEnvironment. Standards Association of Australia, Sydney.

StandardsAustralia/StandardsNewZealand(1992)Australian/NewZealandStandard1337-1992,EyeProtectorsforIndustrialApplications.StandardsAssociationofAustralia/Standards Association of New Zealand,Sydney/Wellington.

StandardsAustralia/StandardsNewZealand(1994)Australian/NewZealandStandard2210-1994,OccupationalProtectiveFootwear.StandardsAssociationofAustralia/StandardsAssociation of New Zealand,Sydney/Wellington.

StandardsAustralia/StandardsNewZealand(1998)Australian/NewZealandStandard2161.2-1998,Occupationalprotectivegloves,Part2:Generalrequirements.StandardsAssociation of Australia, Sydney.

Thompson WR &Weil CS (1952) Biometrics, 8 : 51-54.

USEnvironmentProtectionAgency(1998)ASTEREcotoxicityprofile:Ethyl2-cyclohexylpropionate-[CASNo2511-00-4],ProjectNo.UnitedStatesEnvironmentalProtectionAgency,OfficeofResearchandDevelopment,NationalHealthandEnvironmentalEffectsResearch Laboratory,Mid-Continent EcologyDivision.