Cell line: HES2 p42 + 4

Morphology of HES: large tight colonies, little diff

Morphology of feeders: sparse, OK

Apoptosis: nd - + ++ +++ ++++ minimal

Mitosis: nd - + ++ +++ ++++

Antibody / Intensity / Distribution / % of colonies / % of cells within colony / Comments
1. A2B5 / -
2. NCAM / -
3. GCTM2 / +++ / PC / 100 / 10 / Large colonies positive at edge
Smaller cells positive throughout
4. TG343 / +++ / PC / 100 / 90 / Large colonies positive at edge
Smaller cells positive throughout
5. SSEA1 / + / CS / 10 / ??
6. SSEA3 / -
7. SSEA4 / -
8. TG30 / +++ / CS / 100 / 90
9.Thy1 / +++ / CS / 100 / 100
10. W6/32 / - / -
11.TRA-1-81 / +++ / PC / 100 / 60 / Large colonies at periphery, small colonies positive throughout
12. TRA-1-85 / +++ / CS, cyto / 100 / 100
13. TRA-2-49 / +++ / CS / 100 / 80
14. TRA-2-54 / +++ / CS, cyto / 100 / 100
15. GD2 / -
16. GD3 / +++ / Cyto, granular ? mito / 100 / 90 / Cross react with feeders
17. TRA-1-60 / +++ / PC / 100 / 80 / Small colonies positive throughout large colonies stronger at periphery
18. iso control

Intensity: - (negative); + (faint); ++ (weak); +++ (strong); ++++ (very strong)

Distribution: cs (cell surface); pc (pericellular); cyto (cytoplasmic); sk (cytoskeletal)

nd: not determined

Cell line: HES2p 42 + 9

Morphology of HES: small to intermediate tight colonies, slight degree of differentiation

Morphology of feeders: ok sparse

Apoptosis: nd - + ++ +++ ++++

Mitosis: nd - + ++ +++ ++++

Antibody / Intensity / Distribution / % of colonies / % of cells within colony / Comments
1. A2B5 / -
2. NCAM / -
3. GCTM2 / +++ / PC / 100 / 70 / Small colonies positive throughout
4. TG343 / +++ / PC / 100 / 60 / Small colonies positive throughout. Large colonies positive at edge
5. SSEA1 / -
6. SSEA3 / -
7. SSEA4 / -
8. TG30 / ++ / CS / 100 / 50
9.Thy1 / ++ / CS / 100 / 70
10. W6/32 / -
11.TRA-1-81 / +++ / PC / 100 / 60 / Small colonies positive throughout. Large colonies positive at edge
12. TRA-1-85 / +++ / CS / 100 / 90
13. TRA-2-49 / - / CS / 100 / 60
14. TRA-2-54 / +++ / CS / 100 / 100
15. GD2 / -
16. GD3 / -
17. TRA-1-60 / +++ / PC / 100 / 20 / Small colonies positive throughout
18. iso control / -

Intensity: - (negative); + (faint); ++ (weak); +++ (strong); ++++ (very strong)

Distribution: cs (cell surface); pc (pericellular); cyto (cytoplasmic); sk (cytoskeletal)

nd: not determined