Supplementary data

Supplementary Table S1 Primers for quantitative RT-PCR analysis

Gene / Forward primer (5′–3′) / Reverse primer (5′–3′)
OsIRL1 / GTGGACACCTTAACCCTGCTG / TGTCTAGCTTATGCTCCTCGTTC
OsIRL2 / GGTGCTTTCATCCGTATGTACAGA / AGGAATCAGCGAAGGTAAACACAC
OsIRL3 / ATCGTGAACAAAGGCGTGGA / TTCCGTTCTTCCTCGAGCAAG
OsIRL4 / GCATGACCGGATAGACAATGC / AAGCCCCAAGTCATGAGCAGA
OsIRL5 / TGCTGCAAGAGGAACAAACCA / TGCAACAACCAAACAGCGAG
OsIRL6 / CCTGGAGTCCATTTATCCCCA / CCGCGGATAAAATGAGGAGAA
OsIRL7 / CGTCAGCTTCCCCAAAACCTA / TGGTCCATCGAGATTGGATTG
OsIRL8 / TGAGTGCATTCGAGCAACCA / CCACAGCCCCATACTTCTTCA
Ubiquitin / AACCAGCTGAGGCCCAAGA / ACGATTGATTTAACCAGTCCATGA


Supplementary Table S2 Collection of T-DNA or Tos17 insertion lines of OsIRL genes in rice

Gene / Insertion line / Insertion position / Mutagen / Source
OsIRL1 / 03Z11DQ39 / intron / T-DNA / Rice Mutant Database (http://rmd.ncpgr.cn/)
OsIRL3 / SHIP_ZSF5943 / exon / T-DNA / T-DNA Insertion Population (http://ship.plantsignal.cn/links.do)
OsIRL6 / NE8549 / exon / Tos17 / NIAS Rice Tos17 Insertion Mutant Database
OsIRL7 / ND4567 / intron / Tos17 / NIAS Rice Tos17 Insertion Mutant Database
OsIRL7 / NC0535 / exon / Tos17 / NIAS Rice Tos17 Insertion Mutant Database
OsIRL8 / 3A-13807 / exon / T-DNA / POSTECH, Rice T-DNA Insertion Sequence Database (http://signal.salk.edu/cgi-bin/RiceGE/)


Supplementary Table S3 List of mutant alleles and primers for genotyping

Gene / Mutant / Line / Genotyping primer (5′–3′)
OsIRL1 / osirl1 / 03Z11DQ39 / F: CAACCAGAGCATACGCACAA
R: CCACTTCACCGTACCCACAT
T: AATCCAGATCCCCCGAATTA
OsIRL3 / osirl3 / SHIP_ZSF5943 / F: GTGACTGATTGGTGCAACTCC
R: TGCTGTGCTACCCCTACTCG
T: ATAGGGTTTCGCTCATGTGTTGAGC
OsIRL6 / osirl6 / NE8549 / F: CCTGTGCTGCTACCGATTTT
R: GCGACTACCCTTGTTCTTCA
T: ATTGTTAGGTTGCAAGTTAGTTAAGA
OsIRL7 / osirl7-1 / ND4567 / F: AGAAGCAATGAACGACTCCA
R: GGGTACACGATGACACGACA
T: TTGTTAGGTTGCAAGTTAGTTAAGA
osirl7-2 / NC0535 / F: ATCTAGCATAGCCAACACGG
R: ATTGCAGGCAAATGGTAAGT
T: TTGTTAGGTTGCAAGTTAGTTAAGA
OsIRL8 / osirl8 / 3A-13807 / F: CATTCCATGCAATTTTGTGG
R: AACGCCTGTAATCGTCATCC
T: ttggggtttctacaggacgtaac

Supplementary Fig.1. Confirmation of T-DNA or Tos17 insertion sites

Genotyping for each T-DNA or Tos17 insertion line by performing two sets of PCRs, one using a pair of gene-specific primers (F and R) and the other using the gene-specific primer (F) and a corresponding T-DNA border primer (T). a, b, c, d, e, and f represent the genotyping of insertion lines for osirl1, osirl3, osirl6, osirl7-1, osirl7-2, and osirl8, respectively. M, homozygous for T-DNA insertion; H, heterozygous; W, wild-type.

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