Molecular biology mobile lab supply list

Supplies / Quantity suggested / Quantity included / Quantity needed
1-200 ul tips / 8 boxes
200-1000 ul tips / 8 boxes
p20 micropipettes / 8
p200 micropipettes / 8
p2000 micropipettes / 8
Small gloves / 1 box
Medium gloves / 1 box
Large gloves / 1 box
1.5 ml tube rack / 8
PCR tube rack / 8
0.5 ml tubes / 1 bag
1.5 ml tubes / 1 bag
Boiling caps / 1 bag
Heat block base / 1
1.5 ml tube holder for heat block / 2
15 ml tube holder for heat block / 2
Thermometer / 1
E-gel power bases and adapters / 6
2% E-gels / 1 box
Blue light box / 1
Handheld UV light / 1
Digital camera & memory card / 1
Camera hood / 1
Timer / 1
Microcentrifuge / 2
Permanent markers / 8
Waste containers / 10

Human DNA labs (PTC, Alu, Mitochondrial)

Supplies / Amount per student / 30 students / 60 students / 100 students
Paper cups / 1 / 30 / 60 / 100
15 ml conical tubes / 1 / 30 / 60 / 100
Bottle of water / 1 bottle for 50 students / 1 / 2 / 2-3
Tubes of salt (4.5g) / 1 tube per bottle of water / 1 / 2 / 2-3
Chelex / 100ul / 3 ml / 6 ml / 100 ml
PCR beads in tubes / 1 / 30 / 60 / 100
Relevant primer mix / 23 ul / 690 ul / 1.4 ml / 2.3 ml
SYBR-safe dye / 2 ul / 60 ul / 120 ul / 200 ul
DNA ladder / 10 ul / 10 students / 40 ul / 60 ul / 100 ul
1.5 ml tubes / 3 / 90 / 180 / 300
E-gels / 1 per 9 students / 4 / 7 / 11
water / 2 ul / 60 ul / 120 ul / 200 ul
Styrofoam container and frozen pack / 1 per teacher / 1 / 1 / 1

PTC specific

Supplies / Amount per student / 30 students / 60 students / 100 students
HaeIII enzyme / 1 ul/student / 30 ul / 60 ul / 100 ul
PTC paper and control / 1 each per student / 30 / 60 / 100
50% glycerol / 1 ul/student / 30 ul / 60 ul / 100 ul

GM Foods lab

Supplies / Amount / 30 students 2/group
Round-up spray bottle / 1 bottle / school / 1
WT and RR seeds or leaf tissue / 6-8 seeds of each type / 6-8 seeds
Soil containers and soil / 4 / 4
Sample foods / Small bag of each type
Pestles / 1 per sample or group / 15
Edwards buffer / 1ml/sample or group / 15 ml
Isopropanol / 400 ul / sample or group / 6 + ml
TE or water / 100 ul / sample or group / 1.5 + ml
PCR beads in tubes / 2 tubes / sample or group / 30
Tubulin primer mix / 23 ul / sample or group / ~400 ul
35S primer mix / 23 ul / sample or group / ~400 ul
DNA ladder / 10 ul / 4 groups / 150 ul
SYBR-safe / 2 ul / PCR, 4 ul per group / 60 + ul
E-gels / 1 gel / 4 groups / 4 gels
1.5 ml eppendorf tubes / 3 / sample or group / 45-50
Styrofoam container and frozen pack / 1 / school / 1

Transformation lab

Supplies / Amount
Per group / 30 students 4/group
Bacterial starter culture / 1 / school / 1
pGREEN plasmid / 10 ul / 80 ul
Liquid LB / 500 ul / 4 ml
LB plates / 2 +some for starter / 24
LB AMP plates / 2 / 18
Calcium Chloride / 500 ul / 4 ml
Inoculating loop / 2 / 16
Cell spreader / 2 / 16
15 ml snap cap tube / 2 / 16