Microinjection of DNA Or Protein Solution in Cells
cells
DMEM (cuture media)
FCS/DCS (culture media)Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of supervisor / Laura M. Machesky / Assessment Number* / 2035Signature
Assessor / Juana Magdalena / Date of Assessment / 28/02/2002
Signature
Notes A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1 / LOCATION OF THE WORK ACTIVITY / 8th Floor labs2 / PERSONS WHO MAY BE AT RISK
List names where possible
Stewart Sharp, Celine Costa, Isabel Olazabal, Laura Machesky, Dave Knecht
3 / ACTIVITY ASSESSED / Microinjection of DNA or protein solution in cells
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT** / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
cells / Cultures of 10 ml / Biological hazard / See biological COSHH
(Signalling to Cell Motility)
DMEM (cuture media) / 500 ml / Non hazardous
FCS/DCS (culture media) / 25 ml per 500 ml of DMEM / caution / it is recommended that reagents and sera are purchased from suppliers who issue certificates of analysis or results of quality control (QC) testing with
each batch of products
Hepes buffer / Stock concentration of 1M
(50 µl of the stock concentration in
5 ml of culture media)
Final concentration of 10 mM / Caution Toxicology not determined / Avoid contact with skin and eyes as a precaution.
Plasmid DNA preparation / 3 µl (of 1 mg/ml DNA prep) in
100 µl of water / Non hazardous
Fluorescent labelled dextran / Use 5 µl of (1 mg per ml) / Non hazardous
5 / INTENDED USE
Give brief details and attach protocol/instructions
Microinject a plasmid DNA preparation and/or labelled dextran into the cells (grown on coverslips)
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
Accidental breakage of microinjector glass capillaries could cause glass to enter the eye or wound skin
Increased pressure in the microinjector whilst changing the capillarities may also cause them to break
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Unacceptable risk must be prevented by following precautionary measures
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Eye protection must be worn in addition to lab coat and gloves
This will prevent any fllying glass splinters from entering the eye
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
All staff should be trained before using the microinjector, if capillaries are unproperly mounted, they may break and cause injury
10 / MONITORING
Performance of control measures,
Personal exposure / Health Surveillance
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
The capillaries will be disposed of in a sharp safe container and send for incineration
Buffers may be washed down the sink with plenty of running water
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
28.02.2007
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Carefully sweep up glass splinters and discard into sharpsafe
Mop up any spills with absorbant material and discard in a yellow bin for incineration, wash down the area with household bleach or Virkon
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
If glass gets in the eye seek medical advice
In case of spillage on skin, wash with copious amounts of water
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
Carefully sweep up glass splinters and discard into sharpsafe
Mop up any spills with absorbant material and discard in a yellow bin for incineration, wash down the area with household bleach or Virkon
CONTACT PHONE
10.10.00
* Prefix T is used for Teaching Assessment Number.
** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
*** Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.