Supplementary Information
Metatranscriptome analysis reveals hydraulic fracturing wastewater microbial communityactivity
Amit Vikram1, Daniel Lipus1, and Kyle Bibby1,2*
1Department of Civil and Environmental Engineering, and 2Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA
*Corresponding author
Running title: Metatransriptomic analysis of produced water
Supplementary Methods
Geochemical analysis of hydraulic fracturing samples
Temperature and conductivity were assessed on site for FWT and HP2 samples.Temperature and total dissolved solids (TDS)for HP2 and FWT samples were measured on-site and found to be 23.8oC and 11.60 mS for HP2 and 19.1oC and 19.7mS for FWT. Sampling circumstances did not allow on-site temperature and TDS measurements for HP1 samples; however, the air temperature during sampling was approximately 1oC. pH was measured using a Thermo Fisher Education pH meter (Thermo Fisher Scientific, Pittsburgh, PA). TDS concentrations were determined using a Fisher Scientific Accumet AP75 Conductivity/TDS meter (Thermo Fisher Scientific, Pittsburgh, PA).If necessary,produced water samples were filtered through a 0.45 μm membrane filter to remove solids, which may interfere with atomic absorption and ion chromatography analysis. Cation concentrations were measured using a Perkin Elmer Atomic Absorption Spectrometer 1100 (Perkin Elmer, Bridgeville, PA). Anion concentrations were determined using Thermo Scientific ICS-1100 Ion Chromatograph (Thermo Fisher Scientific, Waltham, MA).
16S rRNA Analyses
For 16S rRNA reads, the sequencing reads were processed using QIIME version 1.7.0[1]. Sequences were demultiplexed and quality trimmed (Phred score 20). Operational Taxonomic Units (OTU) were picked using the pick_closed_reference_otus.py python script using UCLUST [2] against the 2013 GreenGenes corset gg_99_otus.fasta reference database with a 97% sequence similarity[3]. To remove bias introduced through varying number of sequences, 5000 sequences successfully assigned to OTUs were randomly selected for each sample and used for downstream analysis. Average microbial abundance data values were calculated based on OTU data from both replicates. Observed Species, Chao1 and Shannon were calculated from OTU tables.
Fig. S1. Normalized transcript abundances of detected functional categories in (A) FWT, (B) HP1 and (C) HP2. X-axis spacing provided for resolution. Panel A shows selected genes involved in sulfate reduction (red), stress response (green) and methanogenesis (brown) in FWT.
Fig. S2. Domain level distribution of HP1, HP2 and FWT metatranscriptomes
Fig. S3. Phylum level distribution of HP1, HP2 and FWT metatranscriptomes
Table S1. Sampling details and chemical parameters of the collected produced water samples
Sample / Truck (FWT) / Pond (HP2) / Dec Pond (HP1)Sampling date / Jun-14 / Jun-14 / Dec-13
pH / 6.53 / 7.27 / 7.35
Concentration (ppm)
TDS / 52500.0 / 5300.0 / 18500.0
Calcium / 6360.0 / 4850.0 / 1691.0
Sodium / 18300.0 / 1720.0 / 5272.0
Barium / 62.5 / 5.0 / 14.6
Strontium / 727.0 / 39.2 / 1051.3
Iron / 18.3 / 0.9 / 4.2
Magnesium / 449.0 / 40.0 / 193.0
Manganese / 2.0 / BDL / 0.3
Chloride / 37600.0 / 3400.0 / 13867.0
Sulfate / 3.7 / 71.5 / 66.5
Temp / 19.1 / 23.8 / N.A.
Table S2. List of the organisms used to create Methenyl-tetrahydromethanopterin cyclohydrolase(mch) database
Organism name / Entrez GeneID / Organism name / Entrez GeneIDArchaeoglobus fulgidus DSM 4304 / 1485158 / Methanococcus maripaludis C6 / 5737466
Archaeoglobus profundus DSM 5631 / 8739662 / Methanococcus maripaludis C7 / 5328181
Archaeoglobus veneficus SNP6 / 10395144 / Methanococcus maripaludis S2 / 2762185
Burkholderia xenovorans LB400 / 4007071 / Methanococcus maripaludis X1 / 10982765
Ferroglobus placidus DSM 10642 / 8778108 / Methanococcus vannielii SB / 5325664
Halalkalicoccus jeotgali B3 / 9418706 / Methanococcus voltae A3 / 9275599
Haloarcula hispanica ATCC 33960 / 11051034 / Methanocorpusculum labreanum Z / 4794649
Haloarcula hispanica N601 / 17828457 / Methanoculleus marisnigri JR1 / 4847398
Haloarcula marismortui ATCC 43049 / 3128337 / Methanohalobium evestigatum Z-7303 / 9347253
Halobacterium salinarum R1 / 5953669 / Methanohalophilus mahii DSM 5219 / 8983305
Haloferax mediterranei ATCC 33500 / 13028756 / Methanolobus psychrophilus R15 / 13845733
Haloferax volcanii DS2 / 8924970 / Methanomethylovorans hollandica DSM 15978 / 14407338
Halogeometricum borinquense DSM 11551 / 9992833 / Methanopyrus kandleri AV19 / 1476726
Halomicrobium mukohataei DSM 12286 / 8411342 / Methanoregula boonei 6A8 / 5411392
Halophilic archaeon DL31 / 11094524 / Methanosaeta thermophila PT / 4462075
Halopiger xanaduensis SH-6 / 10796011 / Methanosalsum zhilinae DSM 4017 / 10822984
Haloquadratum walsbyi DSM 16790 / 4194249 / Methanosarcina acetivorans C2A / 1473598
Halorhabdus tiamatea SARL4B / 16486852 / Methanosarcina barkeri str. Fusaro / 3624695
Halorhabdus utahensis DSM 12940 / 8383420 / Methanosarcina mazei Go1 / 1480995
Halorubrum lacusprofundi ATCC 49239 / 7400450 / Methanosarcina mazei Tuc01 / 14656073
Haloterrigena turkmenica DSM 5511 / 8742812 / Methanosphaera stadtmanae DSM 3091 / 3855482
Halovivax ruber XH-70 / 14376579 / Methanosphaerula palustris E1-9c / 7272003
Mesorhizobium ciceri biovar biserrulae WSM1271 / 10119751 / Methanospirillum hungatei JF-1 / 3925006
Methanobrevibacter smithii ATCC 35061 / 5216913 / Methanospirillum hungatei JF-1 / 3923282
Methanobrevibacter sp. AbM4 / 15798695 / Methanothermobacter thermautotrophicus str. Delta H / 1471181
Methanocaldococcus fervens AG86 / 8366030 / Methanothermococcus okinawensis IH1 / 10773748
Methanocaldococcus jannaschii DSM 2661 / 1452545 / Methanothermus fervidus DSM 2088 / 9961969
Methanocaldococcus sp. FS406-22 / 8804480 / Methylobacillus flagellatus KT / 4000913
Methanocaldococcus vulcanius M7 / 8513704 / Methylobacterium radiotolerans JCM 2831 / 23619178
Methanocella arvoryzae MRE50 / 5143581 / Methylomicrobium alcaliphilum 20Z / 11360471
Methanocella arvoryzae MRE50 / 5143584 / Natrialba magadii ATCC 43099 / 8823024
Methanocella conradii HZ254 / 11972129 / Natrinema pellirubrum DSM 15624 / 14334027
Methanocella conradii HZ254 / 11971188 / Natrinema sp. J7-2 / 13353014
Methanocella paludicola SANAE / 8681767 / Natronobacterium gregoryi SP2 / 14209527
Methanocella paludicola SANAE / 8681138 / Natronococcus occultus SP4 / 14402090
Methanococcoides burtonii DSM 6242 / 3997983 / Natronomonas pharaonis DSM 2160 / 3702899
Methanococcus aeolicus Nankai-3 / 5327209 / Rhodopirellula baltica SH 1 / 1792000
Methanococcus maripaludis C5 / 4928850 / Salinarchaeum sp. Harcht-Bsk1 / 16181594
Table S3: RPKM values and putative identification of Methanococcus maripaludis S2 genes expressed in FWT
Gene symbol / RPKM / Gene Name / SP PIR KEYWORDSaEF-1 alpha / 1340.95 / elongation factor 1-alpha
aEF-2 / 303.92 / Elongation factor 2 / complete proteome, cytoplasm, elongation factor, GTP-binding, nucleotide-binding, protein biosynthesis
aIF2 gamma / 457.59 / translation initiation factor IF-2 subunit gamma
aksA / 228.69 / MmarC5_1522; MMP0153; MmarC6_0797; MmarC7_1153 / Acyltransferase, complete proteome, transferase
alaS / 111.50 / Alanyl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, nucleotide-binding, protein biosynthesis, RNA-binding, tRNA-binding
arsA / 191.84 / MMP0163 / complete proteome, hydrolase
aspC / 375.50 / Aspartyl-tRNA synthetase / Aminoacyl-tRNA synthetase, Aminotransferase, ATP-binding, complete proteome, cytoplasm, ligase, magnesium, metal-binding, nucleotide-binding, protein biosynthesis, transferase
aspS / 176.40 / Aspartyl-tRNA synthetase
atpA / 169.62 / V-type ATP synthase alpha chain / ATP synthesis, ATP-binding, complete proteome, Hydrogen ion transport, hydrolase, ion transport, nucleotide-binding, transport
atpB / 310.62 / V-type ATP synthase beta chain / ATP synthesis, complete proteome, Hydrogen ion transport, ion transport, transport
atpI / 225.44 / MMP1039 / complete proteome, hydrolase
atpK / 2530.06 / MMP1040 / complete proteome, hydrolase, signal
bioF / 238.55 / MMP1574 / Acyltransferase, complete proteome, pyridoxal phosphate, transferase
carB / 112.47 / MMP1013 / complete proteome, ligase
cobA / 3687.60 / MMP0966 / complete proteome, methyltransferase, transferase
cofD / 1249.03 / LPPG:FO 2-phospho-L-lactate transferase / complete proteome, magnesium, transferase
cofE / 2887.88 / F420-0:gamma-glutamyl ligase / complete proteome, GTP-binding, ligase, manganese, nucleotide-binding, potassium
DP2 / 78.18 / DNA polymerase II large subunit / complete proteome, DNA replication, DNA-binding, DNA-directed DNA polymerase, exonuclease, hydrolase, multifunctional enzyme, nuclease, nucleotidyltransferase, transferase
ehaJ / 566.36 / MMP1457 / complete proteome
eno / 231.01 / Enolase / complete proteome, cytoplasm, glycolysis, lyase, magnesium, metal-binding, phosphoprotein, Secreted
flaC / 295.80 / MMP1669 / complete proteome, flagellum
frdA / 266.33 / Fumarate reductase/succinate dehydrogenase, flavoprotein subunit
fruB / 899.10 / MMP1385 / complete proteome, oxidoreductase
fruG / 1352.66 / MMP1384 / 4fe-4s, complete proteome, iron, iron-sulfur, metal-binding, oxidoreductase, signal
ftr / 292.09 / MMP1609 / Acyltransferase, complete proteome, transferase
ftsZ1 / 643.54 / Cell division protein ftsZ / cell cycle, cell division, complete proteome, GTP-binding, hydrolase, nucleotide-binding, septation
ftsz2 / 181.36 / MMP1500 / cell division, complete proteome, GTP-binding, nucleotide-binding
fwdB / 330.61 / Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B / complete proteome, methanogenesis, oxidoreductase, selenium, selenocysteine, tungsten
glgP / 339.74 / MMP1220 / complete proteome, glycosyltransferase, transferase
glnA / 148.49 / Glutamine synthetase / ATP-binding, complete proteome, cytoplasm, ligase, nucleotide-binding
gltB / 259.79 / MMP0081 / complete proteome, oxidoreductase
gltX / 636.71 / Glutamyl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, nucleotide-binding, protein biosynthesis
guaA_1 / 2667.88 / GMP synthase subunit A
hdrA_1 / 540.47 / Heterodisulfide reductase, subunit A
hdrA_2 / 1158.32 / Heterodisulfide reductase, subunit A
hdrB1 / 219.79 / MMP1155 / complete proteome, oxidoreductase
hdrB2 / 338.66 / MMP1053 / complete proteome, oxidoreductase
hdrC1 / 630.52 / MMP1154 / complete proteome, oxidoreductase
hemL / 310.17 / Glutamate-1-semialdehyde 2,1-aminomutase / complete proteome, cytoplasm, Isomerase, porphyrin biosynthesis, pyridoxal phosphate
hisB / 579.20 / Imidazoleglycerol-phosphate dehydratase / amino-acid biosynthesis, complete proteome, cytoplasm, Histidine biosynthesis, lyase
hisC / 1040.86 / Histidinol-phosphate aminotransferase / amino-acid biosynthesis, Aminotransferase, complete proteome, Histidine biosynthesis, pyridoxal phosphate, transferase
hisF / 243.14 / Imidazole glycerol phosphate synthase subunit hisF / amino-acid biosynthesis, complete proteome, cytoplasm, Histidine biosynthesis, lyase
hisS / 1220.36 / Histidyl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, nucleotide-binding, protein biosynthesis
hmd / 373.95 / MMP0127 / complete proteome, methanogenesis, one-carbon metabolism, oxidoreductase
HMmA / 6507.53 / MMP0386 / complete proteome
hsp60 / 813.44 / MMP1515 / ATP-binding, Chaperone, complete proteome, nucleotide-binding
hypD / 153.22 / MMP0289 / complete proteome
hypE / 462.33 / MMP0274 / complete proteome
ileS / 320.66 / Isoleucyl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, metal-binding, nucleotide-binding, protein biosynthesis, zinc
ilvB / 338.66 / Acetolactate synthase / amino-acid biosynthesis, branched-chain amino acid biosynthesis, complete proteome, magnesium, metal-binding, thiamine pyrophosphate, transferase
ilvC / 267.38 / Ketol-acid reductoisomerase / amino-acid biosynthesis, branched-chain amino acid biosynthesis, complete proteome, NADP, oxidoreductase
ilvD / 702.72 / Dihydroxy-acid dehydratase / 4fe-4s, amino-acid biosynthesis, branched-chain amino acid biosynthesis, complete proteome, iron, iron-sulfur, lyase, metal-binding
infB / 129.28 / Probable translation initiation factor IF-2 / complete proteome, GTP-binding, Initiation factor, nucleotide-binding, protein biosynthesis
korA / 1489.22 / MMP0003 / complete proteome, oxidoreductase
korB / 415.90 / MMP1316 / complete proteome, oxidoreductase
leuA / 150.37 / MMP1063 / Acyltransferase, complete proteome, transferase
leuC / 624.72 / MMP1149 / complete proteome, lyase
leuS / 104.15 / Leucyl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, nucleotide-binding, protein biosynthesis
lysC / 943.52 / Aspartokinase / amino-acid biosynthesis, complete proteome, kinase, transferase
manB / 328.35 / MMP1372 / complete proteome, Isomerase, magnesium, metal-binding
mcrA / 718.88 / MMP1559 / complete proteome, oxidoreductase
mcrB / 896.98 / MMP1555 / complete proteome, oxidoreductase
mcrG / 1949.77 / MMP1558 / complete proteome, oxidoreductase
mer / 964.98 / MMP0058 / complete proteome
MMP0020 / 1791.86 / Putative nickel-responsive regulator / complete proteome, DNA-binding, metal-binding, nickel, Transcription, transcription regulation
MMP0033 / 189.34 / MMP0033 / ATP-binding, complete proteome, DNA-binding, nucleotide-binding, Transcription, transcription regulation
MMP0040 / 341.94 / MMP0040 / complete proteome
MMP0050 / 881.82 / Riboflavin synthase, beta subunit
MMP0072 / 981.11 / MMP0072 / complete proteome
MMP0083 / 215.11 / MMP0083 / 4fe-4s, complete proteome, iron, iron-sulfur, metal-binding
MMP0093 / 1128.86 / 50S ribosomal protein L21e / complete proteome, ribonucleoprotein, ribosomal protein
MMP0103 / 553.14 / Pyridoxal biosynthesis lyase pdxS / complete proteome, lyase, pyridoxal phosphate
MMP0176 / 546.83 / MMP0176 / ATP-binding, cell division, complete proteome, nucleotide-binding
MMP0213 / 225.77 / MMP0213 / complete proteome
MMP0249 / 2737.19 / Ribosomal L37 protein
MMP0281 / 648.69 / MMP0281 / complete proteome
MMP0291 / 642.04 / MMP0291 / complete proteome
MMP0325 / 811.06 / Glyceraldehyde-3-phosphate dehydrogenase / complete proteome, cytoplasm, glycolysis, NAD, NADP, oxidoreductase
MMP0335 / 561.16 / MMP0335 / complete proteome
MMP0346 / 295.80 / MMP0346 / complete proteome
MMP0381 / 1092.62 / MMP0381 / complete proteome
MMP0382 / 318.22 / MMP0382 / ATP-binding, complete proteome, nucleotide-binding
MMP0389 / 6034.26 / MMP0389 / 4fe-4s, complete proteome, iron, iron-sulfur, metal-binding
MMP0443 / 966.65 / SSU ribosomal protein
MMP0608 / 510.18 / MMP0608 / complete proteome
MMP0667 / 750.87 / SSU ribosomal protein S2
MMP0686 / 405.23 / 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase / amino-acid biosynthesis, aromatic amino acid biosynthesis, complete proteome, lyase, Schiff base
MMP0694 / 452.25 / MMP0694 / complete proteome
MMP0708 / 700.18 / UPF0264 protein MMP0708 / complete proteome
MMP0720 / 404.49 / MMP0720
MMP0788 / 276.19 / MMP0788 / complete proteome, signal
MMP0865 / 118.19 / MMP0865 / Aminotransferase,complete proteome,pyridoxal phosphate,transferase
MMP0890 / 207.06 / MMP0890 / ATP-binding,complete proteome, helicase, hydrolase, nucleotide-binding
MMP0940 / 1613.33 / MMP0940 / complete proteome
MMP1024 / 451.23 / MMP1024 / ATP-binding, complete proteome, DNA-binding, nucleotide-binding
MMP1036 / 945.89 / MMP1036 / complete proteome
MMP1091 / 1720.01 / MMP1091 / complete proteome, nucleotidyltransferase, transferase
MMP1116 / 2017.15 / MMP1116 / complete proteome, glycosidase, hydrolase
MMP1122 / 562.99 / MMP1122 / complete proteome
MMP1135 / 1630.88 / MMP1135 / complete proteome,
MMP1197 / 696.87 / MMP1197 / complete proteome, signal, transmembrane, transport
MMP1199 / 232.90 / MMP1199 / complete proteome
MMP1207 / 1239.03 / 30S ribosomal protein S6e / complete proteome, ribonucleoprotein, ribosomal protein
MMP1211 / 663.77 / UPF0219 protein MMP1211 / complete proteome
MMP1215 / 134.64 / Probable cobyric acid synthase / cobalamin biosynthesis, complete proteome, glutamine amidotransferase
MMP1218 / 585.05 / MMP1218 / complete proteome
MMP1236 / 892.16 / UPF0280 protein MMP1236 / complete proteome
MMP1250 / 856.05 / MMP1250 / complete proteome
MMP1265 / 280.07 / Glutamyl-tRNA(Gln) amidotransferase subunit E / ATP-binding, complete proteome, ligase, nucleotide-binding, protein biosynthesis
MMP1268 / 261.07 / MMP1268 / complete proteome
MMP1289 / 890.11 / Ribosaomal protein L10E
MMP1305 / 401.85 / MMP1305 / complete proteome
MMP1314 / 348.44 / MMP1314 / complete proteome
MMP1345 / 751.44 / MMP1345 / complete proteome, transferase
MMP1348 / 704.83 / MMP1348 / complete proteome
MMP1356 / 280.96 / MMP1356 / complete proteome
MMP1359 / 129.14 / MMP1359 / complete proteome
MMP1368 / 526.80 / 30S ribosomal protein S7P / complete proteome, ribonucleoprotein, ribosomal protein, RNA-binding, rRNA-binding
MMP1387 / 388.17 / UPF0103 protein MMP1387 / complete proteome
MMP1392 / 115.00 / UPF0027 protein MMP1392 / complete proteome
MMP1400 / 2011.42 / MMP1400 / complete proteome
MMP1406 / 3007.36 / Protein translation factor SUI1 homolog / complete proteome, protein biosynthesis, translation regulation
MMP1418 / 1140.50 / LSU Ribosomal protein L18P
MMP1442 / 1382.85 / MMP1442 / complete proteome, DNA-binding, Transcription, transcription regulation
MMP1447 / 1921.71 / MMP1447 / complete proteome
MMP1490 / 259.28 / L-seryl-tRNA(Sec) kinase / ATP-binding, complete proteome, kinase, nucleotide-binding, transferase
MMP1510 / 1306.03 / Asp-tRNA Asn/Glu-tRNA Gln amidotransferase subunit A
MMP1525 / 394.16 / MMP1525 / complete proteome
MMP1539 / 709.91 / MMP1539 / complete proteome
MMP1546 / 275.42 / LSU Ribosomal protein L2P
MMP1611 / 925.57 / MMP1611 / complete proteome
MMP1615 / 4646.38 / MMP1615 / complete proteome
MMP1626 / 217.77 / MMP1626 / complete proteome, signal
MMP1640 / 408.72 / S-adenosylmethionine synthetase / ATP-binding, complete proteome, magnesium, nucleotide-binding, one-carbon metabolism, transferase
MMP1709 / 1647.65 / LSU Ribosomal protein L44E
MMP1712 / 372.49 / MMP1712 / complete proteome, DNA-binding, Transcription, transcription regulation
MMP1713 / 210.72 / MMP1713 / complete proteome
mtd / 875.47 / F420-dependent methylenetetrahydromethanopterin dehydrogenase / complete proteome, methanogenesis, one-carbon metabolism, oxidoreductase
mtrA / 276.57 / Tetrahydromethanopterin S-methyltransferase subunit A / cell membrane, cobalt, complete proteome, membrane, methanogenesis, methyltransferase, one-carbon metabolism, transferase, transmembrane
mtrD / 281.26 / Tetrahydromethanopterin S-methyltransferase subunit D / cell membrane, complete proteome, membrane, methanogenesis, methyltransferase, one-carbon metabolism, transferase, transmembrane
mtrH / 345.71 / Tetrahydromethanopterin S-methyltransferase subunit H / complete proteome, methanogenesis, methyltransferase, one-carbon metabolism, transferase
nrdD / 172.18 / MMP0227 / complete proteome, oxidoreductase,
or900 / 348.44 / MMP1565 / complete proteome, methyltransferase, transferase
P1 / 1770.05 / LSU Ribosomal protein L12A
porC / 1750.05 / MMP1507 / complete proteome, oxidoreductase, pyruvate
ppsA / 247.78 / MMP1094 / complete proteome, pyruvate, transferase
pth2 / 567.32 / Peptidyl-tRNA hydrolase / complete proteome, cytoplasm, hydrolase
purB / 368.76 / MMP0971 / complete proteome, lyase
purM_2 / 1770.05 / AIR synthase related protein: Phosphoribosylformylglycinamidine cyclo ligase
purT / 540.34 / Phosphoribosylglycinamide formyltransferase 2 / ATP-binding, complete proteome, lyase, magnesium, metal-binding, nucleotide-binding, purine biosynthesis, transferase
pycA / 176.30 / MMP0341 / complete proteome, ligase, pyruvate
pycB / 504.62 / MMP0340 / complete proteome, ligase, pyruvate
radA / 239.75 / DNA repair and recombination protein radA / 3d-structure, ATP-binding, complete proteome, DNA damage, DNA recombination, DNA-binding, nucleotide-binding
RNaseP RNA / 3318.84 / RNaseP RNA
rpl15 / 2269.29 / 50S ribosomal protein L15e / complete proteome, ribonucleoprotein, ribosomal protein
rplA / 723.74 / LSU Ribosomal protein L1P
rplN / 1247.69 / LSU Ribosomal protein L14P
rpoA1 / 1168.43 / DNA-directed RNA polymerase / complete proteome, DNA-directed RNA polymerase, nucleotidyltransferase, Transcription, transferase
rpoB1 / 831.52 / DNA-directed RNA polymerase / complete proteome, DNA-directed RNA polymerase, hydrolase, nucleotidyltransferase, Transcription, transferase
rpoB2 / 199.13 / MMP1361 / complete proteome, DNA-directed RNA polymerase, nucleotidyltransferase, transferase
rpsE / 587.41 / SSU ribosomal protein S5P
rpsL / 1420.23 / SSU ribosomal protein S12
rpsS / 1092.62 / SSU ribosomal protein S19P
rrnA16S / 26154.95 / RNA_39
rrnA23S / 48766.87 / RNA_38
rrnB16S / 27442.43 / RNA_41
rrnB23S / 48789.32 / RNA_40
rrnB5S / 2020.16 / RNA_47
rrnC16S / 252417.89 / RNA_44
rrnC23S / 48744.43 / RNA_42
rrnD5S / 1731.57 / RNA_37
secY / 199.78 / Preprotein translocase secY subunit / complete proteome, protein transport, translocation, transmembrane, transport
serS / 622.95 / Type-2 seryl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, metal-binding, nucleotide-binding, protein biosynthesis, zinc
smc1 / 55.78 / MMP1397 / complete proteome
SRP RNA / 756.74 / RNA_49
sucC / 365.71 / MMP1105 / complete proteome, ligase
sucD / 191.40 / MMP0955 / complete proteome, ligase, phosphoprotein, tricarboxylic acid cycle
tgtA / 493.57 / 7-cyano-7-deazaguanine tRNA-ribosyltransferase / complete proteome, glycosyltransferase, metal-binding, transferase, tRNA processing, zinc
thiC / 362.72 / Thiamine biosynthesis protein thiC / complete proteome, thiamine biosynthesis
thrC / 355.10 / MMP0135 / complete proteome, lyase
top6A / 842.88 / MMP1437 / complete proteome, Isomerase
top6B / 166.36 / MMP0989 / complete proteome, Isomerase
topA / 107.41 / MMP0956 / complete proteome, Isomerase
trm1 / 236.64 / N(2), N(2)-dimethylguanosine tRNA methyltransferase / complete proteome, methyltransferase, s-adenosyl-l-methionine, transferase, tRNA processing
uvrA / 81.34 / MMP0729 / ATP-binding, complete proteome, nucleotide-binding
valS / 424.05 / Valyl-tRNA synthetase / Aminoacyl-tRNA synthetase, ATP-binding, complete proteome, cytoplasm, ligase, nucleotide-binding, protein biosynthesis
vhcA / 257.82 / MMP0823 / complete proteome, metal-binding, nickel, oxidoreductase
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