Meiosis Pipe Cleaner Lab
Background: Meiosis is the division of the nucleus (and the chromosomes inside of it) in sex cells. One cell divides to form 4 cells with a lower chromosome number. In males, the process will lead to the formation of sperm cells, and in females, meiosis leads to the formation of one egg cell at a time.
Objective: To model the various stages of Meiosis using two different colors of pipe cleaners.
Materials:
- 2 strands of different color pipe cleaner (2 long strands of each color 2 short strands of each color )
- beads of two different colors
- Colored pencils
- yarn
PART ONE: make a cell with chromosomes
Step 1: Make a cell membrane by placing your yarn in a large circle.
Step 2: Make chromosomes. Link 2 of the long strands of the same color together by twisting the pipe cleaners together (the twist should represent the CENTROMERE)
Step 3: Repeat for small strands of all colors. Once completed with this step you should have two large double chromosomes, and two small double chromosomes.
Step 4: Now add a blue bead to the bottom right leg of your blue chromosomes (X’s) and add a pink bead to the bottom right leg of your pink chromosomes (X’s) These beads represent a section of DNA (gene)
Step 5:Make sure that your homologous chromosomes have found one another and are placed next to one another in the cell. Hint: homologous chromosomes are the same size
1. What are homologous chromosomes?
2. How many sets of homologous chromosomes did you make?
3. Is this cell diploid or haploid? Why?
4. What is the total number of chromosomes at this point? (hint: count the number of X’s)
5. Where do your homologous chromosomes originally inherit (come) from?
6. Draw and label this picture as PROPHASE 1 Label: HOMOLOGOUS CHROMOSOMES, CENTROMERES, TELL ME WHICH COLOR PIPE CLEANER IS MATERNAL AND WHICH IS PATERNAL
PART TWO: Meiosis 1
Step 6: Next you need to demonstrate crossing over. Remove the small blue bead from the bottom of the large blue chromosome and remove the small pink bead from the bottom of the large pink chromosome. Then place the blue bead on the pink chromosome and the pink bead on the blue chromosome. (Repeat for small chromosomes!) Place the homologous chromosomes side by side in the middle of the cell. Draw and label this as Metaphase 1
Step 7: Pull one homologous chromosome to one side of the cell (the right) and the other homologous chromosome to the other side of the cell (the left). Repeat for the other set of homologous chromosomes. You should now have 2 chromosomes on each side of the cell. Draw and label this as Anaphase 1
Step 8: Begin to divide your one yarn cell into 2 by pinching the membrane in slightly. You should have two chromosomes on one side and two chromosomes on the other. (The chromosomes in each daughter cell should be made of different sizes of pipe cleaners!!)
Draw and label this as Telophase 2
PART THREE MEIOSIS 2:
Step 8: Finish dividing the cell membrane into 2 cells by cutting the yarn in half and separating the two cells. This is cytokinesis. Draw and label this as Prophase 2. Label: SISTER CHROMATIDS, CENTROMERES, DAUGHTER CELLS
Step 9: Line up 2 the chromosomes in each cell vertically down the middle. Draw and label this as Metaphase 2.
Step 10: Pull the sister chromatids apart (breaking the bond made by the twisting of the pipe cleaners) and to opposite sides of the cell. Draw and label this as ANAPHASE 2.
Step 11: Pinch the membrane (yarn) in to divide your 2 cells into 4. Draw and label this as TELOPHASE 2. Make sure each sister chromatid is going to be in a separate cell. Cut the yarn to complete cytokinesis.
Analysis Questions: answer in complete sentences
7. How many chromosomes did your original parent cell have (at the beginning of the lab)?
8. Was this parent cell diploid or haploid? How can you tell?
9. How many chromosomes did each of your daughter cells have (at the end of the lab)?
10. Were the daughter cells diploid or haploid? How can you tell?
11. If human somatic cells have 46 chromosomes, how many chromosomes do human gametes have?
12. Are human gametes haploid or diploid?
13. Are human somatic cells haploid or diploid?
14. Are fertilized eggs haploid or diploid?
Conclusion: What are the two types of cell divison? Compare and contrast. Why is it important to have 2 types of cell division?