Measurements of Five Shell Parameters on 61 Specimens of Pisidiumspp. As Described in Section

Appendix I

List of specimens analyzed in the present study. All specimens sequenced in the course of this study hold an individual four-digit DNA collection number of the University Giessen (marked in bold and listed in column ‘code’). Sequences that were taken from GenBank are marked with GB (*Lee & Ó Foighil, 2003; #Guralnick, 2005; §Steiner, unpublished,N/A – Locality data not available). The locality code (‘Loc’) refers to the codes in Figure 2 and is given only for specimens found in the lakes Ohrid (O) and Prespa (P) as well as within the surrounding watershed (W).

Species / Specimen code / Locality / Locality code / Latitude
(in °) / Longitude (in °) / GenBank Accession #
Eupera cubensis / GB01* / Cuba, Havana / AY093549
E. platensis / GB02* / Argentina ,Buenos Aires / AF152026
Musculium argentinum / GB08* / Argentina ,Buenos Aires / AF152034
M. japonicum / GB05* / Japan, EhimePrefecture / AY093550
M. lacustre / GB03* / Germany / AF152035
M. miyadii / GB02* / Russia, Kuril Islands / AY093553
M. partumeium / GB06* / USA, Michigan / AF152036
M. securis / GB09* / USA, Michigan / AF152033
M. transversum / GB01* / USA, Michigan / AY093554
Pisidium adamsi / GBCU* / USA, Michigan / AY093556
GBCX* / USA, Michigan / AF152031
P. amnicum / 5002 / Macedonia, Struga, LakeOhrid shore / O1 / 41.17400 / 20.67643 / EU559086
5003 / Albania, Tušemišta, LakeOhrid shore / O11 / 40.90095 / 20.69390 / EU559087
5004 / Macedonia, Misleschwbo, LakeOhrid shore / O2 / 41.16758 / 20.72645 / EU559088
5052 / MontenegroLake Skutari at CrnojevicaRiver, / 42.308 / 19.087 / EU559089
P. annandalei / 5244 / India, Pithoragarh / 29.55900 / 80.30533 / EU559090
P. casertanum Type I / 5045 / Macedonia, north of Misleschwbo, creek / W4 / 41.18526 / 20.72862 / EU559091
5130 / Germany, Müllrose / 52.24666 / 14.45416 / EU559092
P. casertanum Type II / 5043 / Albania, Tušemišta, wetland / W5 / 40.89623 / 20.71040 / EU559093
5044 / Albania, Tušemišta, wetland / W5 / 40.89623 / 20.71040 / EU559094
5050 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559095
6020 / Spain, Picos de Europa / 43.15388 / –4.84222 / EU559096
6021 / Spain, Picos de Europa / 43.15388 / –4.84222 / EU559097
GB36§ / N/A / DQ062615.1
GB39§ / N/A / DQ062612.1
P. cf. casertanum / 4882 / Macedonia, Kalishta, LakeOhrid shore / O4 / 41.14380 / 20.64993 / EU559098
5015 / Macedonia, North of Struga, DrimRiver / W1 / 41.34999 / 20.62245 / EU559099
5016 / Macedonia, North of Struga, DrimRiver / W1 / 41.34999 / 20.62245 / EU559100
5017 / Macedonia, North of Struga, DrimRiver / W1 / 41.34999 / 20.62245 / EU559101
5051 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559102
P. clarkeanum / 3513 / Nepal, Lamahi / 27.83527 / 82.53972 / EU559103
6018 / Nepal , Ghatgai w Sauraha / 27.55194 / 84.36639 / EU559104
6022 / Nepal, Haripur / 26.55467 / 86.98433 / EU559105
P. compressum / GBAO# / USA / AY957812
GBAP# / USA / AY957811
GBCQ* / USA, Michigan / AY093560
P. dubium / GBDB* / USA, Michigan / AF152027
P. edlaueri / 3523 / Macedonia, Sveti Zaum, LakeOhrid littoral / O8 / 40.93980 / 20.77822 / EU559106
4853 / Macedonia, Sveti Zaum, LakeOhrid littoral / O8 / 40.93980 / 20.77822 / EU559107
4854 / Macedonia, Sveti Zaum, LakeOhrid littoral / O8 / 40.93980 / 20.77822 / EU559108
4858 / Macedonia, Sveti Zaum, LakeOhrid littoral / O8 / 40.93980 / 20.77822 / EU559109
5014 / Macedonia, Struga, LakeOhrid shore / O1 / 41.17400 / 20.67643 / EU559110
5054 / Macedonia, Ohrid town, LakeOhrid littoral / O5 / 41.10111 / 20.80465 / EU559111
5055 / Macedonia, Sveti Naum, LakeOhrid littoral / O9 / 40.91746 / 20.73573 / EU559112
5062 / Macedonia, Kalishta; LakeOhrid littoral / O3 / 41.14998 / 20.66333 / EU559113
P. fallax / GB59# / Colorado, USA / AY957868
GBAC# / USA, Colorado / AY957824
GBAK# / USA, Colorado / AY957816
P. ferrugineum / GB43# / USA / AY957884
GB47# / USA / AY957880
GB49# / USA / AY957878
P. globulare / 5131 / Germany, Uhyst / 51.33000 / 14.48250 / EU559114
P. hallae / GB96# / USA / AY957832
GBAS# / USA / AY957808
GBAV# / USA / AY957805
P. henslowanum / 4801 / Macedonia, Kazan, LakePrespa littoral / P2 / 40.93828 / 20.94855 / EU559115
4802 / Macedonia, Kazan, LakePrespa littoral / P2 / 40.93828 / 20.94855 / EU559116
4861 / Macedonia, Kazan, LakePrespa shore / P3 / 40.93665 / 20.94414 / EU559117
4862 / Macedonia, Kazan, LakePrespa shore / P3 / 40.93665 / 20.94414 / EU559118
4863 / Macedonia, Kazan, LakePrespa shore / P3 / 40.93665 / 20.94414 / EU559119
5011 / Macedonia, Struga, LakeOhrid shore / O1 / 41.17400 / 20.67643 / EU559120
5012 / Macedonia, Struga, LakeOhrid shore / O1 / 41.17400 / 20.67643 / EU559121
5013 / Macedonia, Struga, LakeOhrid shore / O1 / 41.17400 / 20.67643 / EU559122
P. hibernicum / GBCN* / Germany, Heiliges Meer / AY093563
P. insigne / GB89# / N/A / AY957838
P. japonicum / GBCF* / Japan, NaganoPrefecture / AY093571
P. kuiperi / 5243 / Nepal, Tortong / 27.52333 / 87.90833 / EU559123
P. lilljeborgi / 5136 / Norway, Tangstad/Vestvågöy / 68.25166 / 13.65750 / EU559124
GB27§ / N/A / DQ062624
GB46# / USA / AY957881
P. maasseni / 3527 / Macedonia, Konsko, LakePrespa shore / P4 / 40.91137 / 20.98983 / EU559125
3528 / Macedonia, Konsko, LakePrespa shore / P4 / 40.91137 / 20.98983 / EU559126
3529 / Macedonia, Konsko, LakePrespa shore / P4 / 40.91137 / 20.98983 / EU559127
4807 / Macedonia, Island Golem Grad, LakePrespa shore / P6 / 40.87103 / 20.98772 / EU559128
4808 / Lake Prespa, Macedonia / P6 / 40.87103 / 20.98772 / EU559129
4886 / Macedonia, Island Golem Grad, LakePrespa shore / P5 / 40.87187 / 20.98952 / EU559130
4949 / Macedonia, Island Golem Grad, LakePrespa shore / P6 / 40.87103 / 20.98772 / EU559131
P. milium / GB26§ / N/A / DQ062625
GB72# / USA, Colorado / AY957855
GB73# / USA, Colorado / AY957854
GB74# / USA, Colorado / AY957853
GB75# / USA, Colorado / AY957852
GB76# / USA, Colorado / AY957851
GB77# / USA, Colorado / AY957850
GB78# / USA, Colorado / AY957849
GB79# / USA, Colorado / AY957848
GB80# / USA, Colorado / AY957847
GB82# / USA, Colorado / AY957845
GB83# / USA, Colorado / AY957844
GB84# / USA, Colorado / AY957843
GB85# / USA, Colorado / AY957842
GB86# / USA, Colorado / AY957841
GB87# / USA, Colorado / AY957840
GBCM* / Germany, Heiliges Meer / AY093564
GBDA* / USA, Michigan / AF152028
P. moitesserianum / GB24§ / N/A / DQ062627
P. nevillianum / 3510 / Nepal, Lamahi / 27.83527 / 82.53972 / EU559132
3511 / Nepal, Lamahi / 27.83527 / 82.53972 / EU559133
P. nitidum / 3323 / Germany, Zarrentin / 53.58138 / 10.95250 / EU559134
5133 / Germany, Zarrentin / 53.58138 / 10.95250 / EU559135
GB20§ / N/A / DQ062631
GB21§ / N/A / DQ062630
GB69# / USA, Colorado / AY957858
GB70# / USA, Colorado / AY957857
GB71# / USA, Colorado / AY957856
GBCK* / Germany, Heiliges Meer / AY093566
P. nipponense / GBCL* / Japan, GunmaPrefecture / AY093565
P. obtusale / 6026 / Germany, Craula / 51.08027 / 10.48500 / EU559136
6027 / Germany, Craula / 51.08027 / 10.48500 / EU559137
6028 / Germany, Craula / 51.08027 / 10.48500 / EU559138
P. parvum / GBCG* / Japan, EhimePrefecture / AY093570
P. personatum / 4867 / Macedonia, Belina, LakeOhrid littoral / O7 / 41.05781 / 20.79838 / EU559139
4952 / Macedonia, Novo Selo, swamp / W2 / 41.31838 / 20.81576 / EU559140
4953 / Macedonia, Novo Selo, swamp / W2 / 41.31838 / 20.81576 / EU559141
4954 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559142
5046 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559143
5047 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559144
5048 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559145
5049 / Macedonia, Pretor, LakePrespa shore / P1 / 40.97319 / 21.05432 / EU559146
P. pseudosphaerium / 5132 / Germany, Uhyst / 51.33000 / 14.48250 / EU559147
P. supinum / 6019 / Germany, Erpe/Heidemühle / 52.48727 / 13.64439 / EU559148
GB03§ / N/A / DQ062648
GB05§ / N/A / DQ062646
P. subtruncatum / 3525 / Macedonia, Konsko, LakePrespa shore / P4 / 40.91137 / 20.98983 / EU559149
5000 / Macedonia. Struga. LakeOhrid shore / O1 / 41.17400 / 20.67643 / EU559150
5005 / Macedonia. Struga.Drim river / W3 / 41.20516 / 20.67119 / EU559151
5006 / Macedonia. Struga.Drim river / W3 / 41.20516 / 20.67119 / EU559152
5058 / Macedonia. Struga.Drim river / W6 / 40.91393 / 20.74226 / EU559153
4881 / Macedonia. Kazan. LakePrespa shore / P3 / 40.93665 / 20.94414 / EU559154
5059 / Macedonia. Sveti Naum. feeder springs / W6 / 40.91393 / 20.74226 / EU559155
6005 / Italy. Caserta / 41.09786 / 14.33066 / EU559156
6006 / Italy. Caserta / 41.09786 / 14.33066 / EU559157
6009 / Italy. Caserta / 41.09786 / 14.33066 / EU559158
6010 / Italy. Caserta / 41.09786 / 14.33066 / EU559159
6015 / Italy. Caserta / 41.09786 / 14.33066 / EU559160
P. s. recalvum / 4864 / Macedonia. Belina. LakeOhrid littoral / O6 / 41.05795 / 20.79929 / EU559161
4865 / Macedonia. Belina. LakeOhrid littoral / O6 / 41.05795 / 20.79929 / EU559162
P. sterkianum / GBCW* / Argentina. Buenos Aires / AF152032
P. tenuilineatum / 5056 / Albania. Tušemišta. wetland / W5 / 40.89623 / 20.71040 / EU559163
P. variabile / GB60# / USA. Colorado / AY957867
GBAX# / USA. Colorado / AY957803
GBAZ# / USA. Colorado / AY957801
P. ventricosum / GB90# / USA. Colorado / AY957837
GB92# / USA. Colorado / AY957836
GB93# / USA. Colorado / AY957835
P. waldeni / 5140 / Norway. Davggejavrre / 70.50638 / 29.11638 / EU559164
Sphaerium corneum / GB17 / Lithuania. Vilnius / AY792320
S. fabale / GB15* / USA. Michigan / AF152039
S. novaezealandiae / GB07* / New Zealand / AF152047
S. nucleus / GB05* / Ukraine / AY093573
S. occidentale / GB08* / USAMichigan / AF152046
S. rhomboideum / GB16* / USAMichigan / AF152038
S. simile / GB14* / USAMichigan / AF152040
S. striatinum / GB09* / USAMichigan / AF152045
S. tasmanicum / GB06* / Australia. New South Wales / AF152048

Appendix II

Measurements of five shell parameters on 61 specimens of Pisidiumspp. as described in section Materials & methods. Collection numbers refer to ‘specimen code’ in AppendixI.‘Thickest height’ refers to the height of the shell at point of its largest width. All measurements are given in micrometers [µm].

Collection number / Total height / Hinge height / Width / Thickest height / Length
4797 / 2627.21 / 2539.36 / 2113.03 / 1531.79 / 3107.18
4799 / 2506.69 / 2433.88 / 2194.28 / 1435.99 / 3177.69
4801 / 2886.39 / 2824.43 / 2072.31 / 1669.42 / 3580.39
4802 / 2714.88 / 2649.05 / 1960.74 / 1590.91 / 3363.63
4807 / 2002.25 / 1987.61 / 1357.44 / 1223.14 / 2380.46
4808 / 2371.91 / 2334.74 / 1272.73 / 1603.31 / 2608.75
4811 / 2557.86 / 2508.68 / 2149.05 / 1613.80 / 3022.18
4861 / 2597.21 / 2555.95 / 1747.93 / 1462.81 / 3295.46
4862 / 2779.59 / 2699.94 / 1815.75 / 1666.86 / 3410.55
4863 / 2093.24 / 2043.89 / 1233.47 / 1239.67 / 2462.61
4864 / 2184.36 / 2126.61 / 1574.65 / 1272.83 / 2555.68
4865 / 2781.04 / 2702.48 / 1942.19 / 1809.95 / 2990.09
4866 / 2603.33 / 2485.64 / 1967.13 / 1473.23 / 2901.77
4867 / 1335.48 / 1300.61 / 956.88 / 799.91 / 1648.77
4871 / 2603.63 / 2485.45 / 2084.05 / 1614.99 / 3188.19
4872 / 2877.81 / 2798.30 / 2223.69 / 1754.57 / 3443.61
4873 / 2196.55 / 2129.70 / 1569.41 / 1323.49 / 2701.87
4874 / 2581.77 / 2506.96 / 1860.22 / 1581.07 / 3064.85
4875 / 2253.41 / 2247.82 / 1707.93 / 1465.89 / 2773.81
4876 / 1952.96 / 1952.96 / 1308.17 / 1256.40 / 2441.75
4886 / 1640.50 / 1640.50 / 977.70 / 983.61 / 1947.32
4952 / 1646.69 / 1632.23 / 1095.05 / 1002.07 / 1999.91
4953 / 1479.34 / 1456.61 / 904.96 / 909.09 / 1764.47
4954 / 2938.76 / 2880.71 / 2011.10 / 1909.79 / 3653.51
4999 / 2473.92 / 2351.10 / 2312.22 / 1620.06 / 3083.85
5000 / 2171.74 / 2171.74 / 1595.81 / 1380.32 / 2478.39
5001 / 2045.45 / 2045.45 / 1508.26 / 1262.40 / 2423.85
5002 / 3256.26 / 3167.68 / 2355.37 / 2150.83 / 4411.16
5003 / 4481.52 / 4295.91 / 3269.68 / 2821.50 / 5690.75
5004 / 3741.91 / 3633.65 / 2537.76 / 2349.48 / 5056.95
5005 / 1826.45 / 1781.57 / 1295.73 / 1138.48 / 2292.39
5007 / 2121.35 / 2070.64 / 1531.68 / 1305.57 / 2594.44
5011 / 2924.29 / 2846.44 / 2408.11 / 1860.34 / 3677.37
5012 / 2349.99 / 2277.20 / 1669.42 / 1479.34 / 3022.96
5013 / 2231.11 / 2180.23 / 1546.46 / 1362.14 / 2898.76
5014 / 2043.85 / 1968.27 / 1434.27 / 1351.64 / 2323.57
5015 / 3373.97 / 3277.09 / 2235.63 / 2340.92 / 4221.92
5016 / 3165.98 / 3130.32 / 1844.16 / 2123.13 / 4028.53
5017 / 3022.27 / 3000.83 / 1809.83 / 2002.00 / 3669.05
5043 / 2157.03 / 2136.36 / 1264.46 / 1462.81 / 2659.19
5044 / 2280.82 / 2227.07 / 1504.45 / 1444.88 / 2926.79
5046 / 2669.42 / 2607.67 / 1721.07 / 1626.04 / 3312.22
5047 / 2061.19 / 2045.60 / 1200.41 / 1268.60 / 2490.21
5048 / 1971.55 / 1927.39 / 1177.69 / 1322.33 / 2458.45
5049 / 1933.80 / 1919.58 / 1182.58 / 1195.30 / 2390.67
5052 / 3025.23 / 2963.64 / 1801.65 / 2008.61 / 3963.81
5053 / 1922.23 / 1871.79 / 1203.85 / 1187.21 / 2203.95
5056 / 1849.17 / 1832.76 / 1340.95 / 1111.57 / 2212.53
5058 / 2106.61 / 2022.61 / 1669.58 / 1330.68 / 2657.85
5059 / 1876.03 / 1859.51 / 1361.57 / 1266.53 / 2362.22
5060 / 2592.43 / 2525.55 / 2155.85 / 1541.99 / 3082.64
5062 / 1921.56 / 1876.11 / 1378.11 / 1252.13 / 2149.65
5064 / 1888.00 / 1839.03 / 1335.23 / 1256.49 / 2101.32
5129 / 3947.32 / 3863.61 / 2460.06 / 2553.22 / 5162.99
5132 / 2248.45 / 2243.18 / 1585.14 / 1433.21 / 2746.84
5133 / 2659.74 / 2659.74 / 1434.62 / 1747.53 / 2446.06
5135 / 2106.52 / 2069.44 / 1430.40 / 1365.16 / 2411.49
5136 / 2971.60 / 2870.85 / 2194.46 / 1768.67 / 3468.24
5140 / 2001.73 / 1906.87 / 1470.33 / 1277.56 / 2362.99
5229 / 2126.53 / 2126.53 / 1290.06 / 1378.74 / 2433.63
5243 / 1901.80 / 1901.80 / 1057.60 / 1146.31 / 2469.51