Materials and Methods s21

Electronic Supplementary Material 1

Article title: Ecosystem-scale rates of primary production within wetland habitats of the northern San Francisco Estuary.

Journal name: Wetlands

Author names: Risa A. Cohen1, 2*, Frances P. Wilkerson2, Alexander E. Parker2 and Edward J. Carpenter2

Affiliation and email of corresponding author:

1Department of Biology

Georgia Southern University

PO Box 8042

Statesboro, GA 30460, USA

Email:

2Romberg Tiburon Center for Environmental Studies

San Francisco State University

3152 Paradise Dr.

Tiburon, CA 94920, USA
ESM 1. Statistics output

A. Two-factor ANOVA results for phytoplankton primary production.

df F p

Phytoplankton production

Site 5 199.65 <0.0001

Time 7 49.33 <0.0001

Site x Time 35 40.92 <0.0001

B. Kruskal Wallis with the Scheirer-Ray-Hare extension results for water column nutrients, and phytoplankton chlorophyll a concentration.

df H p

Nitrate (NO3-)

Site 5 62.58 <0.001

Time 7 35.08 <0.001

Site x Time 35 45.04 0.1<p<0.5 (n.s.)

Silicate (Si(OH)4)

Site 5 67.16 <0.001

Time 7 39.01 <0.001

Site x Time 35 36.49 0.1<p<0.5 (n.s.)

Orthophosphate (PO4-)

Site 5 75.80 <0.001

Time 7 15.80 0.025<p<0.05

Site x Time 35 48.06 0.05<p<0.1 (n.s.)

Ammonium (NH4+)

Site 5 32.02 <0.001

Time 7 31.44 <0.001

Site x Time 35 75.10 <0.001

Phytoplankton Chlorophyll a

Site 5 35.43 <0.001

Time 7 78.46 <0.001

Site x Time 35 97.34 <0.001

C. Nested ANOVA results for microphytobenthos production and chlorophyll a concentration, and low marsh vegetation production and chlorophyll a concentration.

df F p

Microphytobenthos

Production

Site 5 33.62 <0.0001

Plot[Site] 12 0.78 0.67

Component Variance Component Estimates Percent of Total

Site 227.42 27.14

Plot[Site] -5.91 -0.71

Residual 616.37 73.56

Chlorophyll a

Site 5 11.70 <0.0001

Plot[Site] 12 1.33 0.20

Component Variance Component Estimates Percent of Total

Site 3147.40 16.94

Plot[Site] 217.36 1.17

Residual 15217.03 81.89

Low Marsh Vegetation

Production

Site 4 41.07 <0.0001

Plot[Site] 10 0.45 0.92

Component Variance Component Estimates Percent of Total

Site 1232700 16.99

Plot[Site] -113695 -1.57

Residual 6137334 84.58

Chlorophyll a

Site 4 9.31 <0.0001

Plot[Site] 10 1.04 0.40

Component Variance Component Estimates Percent of Total

Site 1048.88 8.77

Plot[Site] 15.74 0.13

Residual 10893.45 91.10