Margarita Hadjistylli .George K. Roderick .Nathalie Gauthier

SupplementaryMaterial

Title:First report of the Sub-Saharan Africa 2 species of the Bemisia tabacicomplex in the Southern France

Margarita Hadjistylli .George K. Roderick .Nathalie Gauthier

-N. Gauthier 

UMR (INRA/IRD/Cirad/Montpellier SupAgro) Centre de Biologie pour la Gestion des Populations, 755 avenue du Campus Agropolis, CS 30016, F-34988 Montferrier-sur-Lez, France.

e-mail:

-George K. Roderick

Department of Environmental Science, Policy and Management, 130 Mulford Hall, University of California, Berkeley, CA 94720-3114, USA.

e-mail:

-Margarita Hadjistylli

Department of Environmental Science, Policy, and Management, 130 Mulford Hall, University of California, Berkeley, CA 94720-3114, USA

Department of Environment, Ministry of Agriculture, Rural Development and Environment, 1498 Lefkosia, Cyprus

e-mail: ,

Fig. 2. Principal CoordinatesAnalysis (PCA)performed in the Excel-based program GenAlEx 6.1 (Peakall Smouse 2012) using individual microsatellite genotypes (30 femalewhitefliesfromeachsamplegenotyped at 13 loci,Hadjistylli2010). In black squares are genotypes of the F27 sample (Med species) whereas in grey circles are genotypes of the SSA2 species sample. In between the two clusters are the admixed genotypes of the 5 individuals that likely represent hybrids between the twospecies.

Fig. 3. Evolutionary relationships of Bemisia tabaci species complex taxa.

The Neighbor-Joining phylogenetic tree was constructedwith 705 mtCOI sequences, namely the 7 mtCOI sequencesfrom the present study (KP293730- KP293736), 91 homologous sequences from different B. tabaci species collected in the Mediterranean basin (KF870484- KF870574, Gauthier et al. 2014) and all the 607 COI sequences retrieved from including sequences from all members of the B. tabaci species complex as well as related whitefly species used as outgroups.Evolutionary analyses were conducted in MEGA6(Tamura et al. 2013).

The phylogeny containing all 705 mtCOI sequences is shown on the left and the section highlighted in red which clustered the 7 sequences generated in this study is magnified on the right. Bootstrap values over 74 are shown at thecorresponding nodes of that section of tree.

References

Gauthier, N., Clouet, C., Perrakis, A., Kapantaidaki, D., Peterschmitt, M. & Tsagkarakou, A. (2014). Genetic structure of Bemisia tabaci Med populations from home range countries inferred by nuclear and cytoplasmic markers: impact on the distribution of the insecticide resistance genes. Pest Management Science, 70, 1477-1491

Hadjistylli, M. (2010).Global genetics and evolution of invasive biotypes in the whitefly complex Bemisia tabaci. Phd dissertation, University of California, Berkeley.

Peakall, R. & Smouse, P.E. (2012). GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28, 2537–2539.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725-2729.