CURRICULUM VITAE
DR. MUHAMMAD AJMAL
Personal Data:
Father’s Name: / Chaudhry Muhammad Ashraf
Date and Place of Birth: / 31stDec 1975, Gujrat, Pakistan
Nationality: / Pakistani
Home Address: / Ch. M. Hanif Street No. 1, NaiAbadi,
DhokeGujran, Near IqbalMasjid, Chakra Road,
Rawalpindi
Mobile: / +923005347366
Email: / ,
Present Position:
Senior Scientific Officer, Department of Bio Sciences, COMSATS Institute of Information Technology, Park Road, ChakShahzad, Islamabad, Pakistan, since 27th June 2013.
Previous Experience:
Administrator PCR Laboratories, Shifa College of Medicine, ShifaTameer-e-Millat University, Sector H-8/4, Islamabad, From 14th February 2005 to 15th August 2013 (8½ Years).
Academic Record:

Ph.D.(Molecular Genetics),2013Netherlands

M.Phil.(Molecular Biology/Biochemistry),2005Pakistan

M.Sc.(Biochemistry),2001Pakistan

B.Ed.(Biology, chemistry), 1998Pakistan

B.Sc.(Botany, Zoology and Chemistry),1995Pakistan

F.Sc.(Pre-Medical),1994Pakistan

Matric(Science),1991Pakistan

PhD Thesis: “Genetic Basis of Inherited Eye Diseases”. Online link to access PhD thesis: ()

M. Phil Thesis: “Genetic Mapping of The Candidates of Hereditary Deafness Genes”.

M.Sc. Research Report: “Correlation of Serum Cholesterol and Sugar with Age and Gender”.

Conferences and courses attended:

Real Time PCR Workshopheld on 12thAug 2005 at Shifa College of Medicine, Islamabad Pakistan, conducted by BioFlux Corporation, Tokyo, Japan.

BICMAPE Conferenceheld from 28thJul 2009 to 29thJul 2009 at COMSATS Institute of Information Technology, Abbottabad, Pakistan.

Eye Genetics Course:(Bologna, Italy) (28thSep 2011 to 1stOct 2011).

NGS Course: Genomic resequencing:variant detection and interpretation in a diagnostic context (Nijmegen, Netherlands) (4thApr 2012 to 5thApr 2012).

Dutch Ophthalmology PhD Students (DOPS) conference:(Nijmegen, Netherlands) (13thApr 2012 to 14thApr 2012).

Technical Expertise

Next Generation Sequencing, Polymerase Chain Reaction (PCR), Real-Time PCR, Sanger Sequencing, Diagnostic Virology, Homozygosity Mapping, Disease Gene Identification, Poly Acrylamide Gel Electrophoresis (PAGE), Agarose Gel electrophoresis, RFLP and ARMS techniques, Molecular Cloning, Extraction of Plasmid and Genomic DNA from Prokaryotes, Extraction of Genomic DNA from Eukaryotes etc.

Bioinformatic Tools:Easylinkage for linkage studies, VectorNTI and BioEdit for Sanger Sequencing Data Analysis, IGV for Exome Sequencing Data Analysis, Alamut mutation detection software, Online Tools; HomozygosityMapper, Mutation Taster, Mutalyzer, Primer3, NEB Cutter, NetGene2, UCSC genome browser, Ensembl genome browser, Galaxy project etc.

Statistical Data Analysis: SPSS, MS Excel, R packages.

Computer Application: MS Office Package, Corel Draw, End Note, Reference Manager, Adobe photoshop etc.

Administrative Skills

Problem Solving related to the diagnostics and research, handling of critical issues, communication, staff management, organization, job prioritization, information handling etc.

Publications:

  1. Shafique S, Siddiqi S, Schraders M,Oostrik J, Ayub H,Bilal A, Ajmal M, Seco CZ, Strom TM,Mansoor A, Mazhar M, Shah STA, Hussain A, Azam M, Kremer H,Qamar R. Genetic Spectrum of Autosomal Recessive Non-Syndromic Hearing Loss in Pakistani Families. (Under revision).
  2. Ajmal M, Khan MI, Neveling K, Khan YM, Azam M, Waheed NK,Hamel C,Ben-Yosef T,De Baere E, Koenekoop RK, Collin RWJ, Qamar R, Cremers FPM.A missense mutation in the splicing factor gene DHX38 is associated with early-onset retinitis pigmentosa with macular coloboma. J Med Genet 2014; (doi: 10.1136/jmedgenet-2014-102316.).Impact Factor = 5.703
  3. Khan MI,Azam M, Ajmal M, Collin RWJ,den Hollander AI,Cremers FPM, Qamar R. The molecular basis of retinal dystrophies in Pakistan. Genes 2014;176-195. Impact Factor = 0.000
  4. Cremers FPM, den Dunnen JT, Ajmal M, HussainA, PreisingMN, DaigerSP, QamarR.Comprehensive Registration of DNA Sequence Variants Associated with Inherited Retinal Diseases in Leiden Open Variation Databases. Hum Mutat. 2014; 35:147-148.Impact Factor = 5.213
  5. Ajmal M, Khan MI, Neveling K, Tayyab A, Jaffar S, Sadeque A, Ayub H, Abbasi NM, Riaz M, Micheal S, Gilissen C, Ali SHB, Azam M, Collin RWJ, Cremers FPM,Qamar R. Exome sequencing identifies a novel and a recurrentBBS1mutation in Pakistani families with Bardet-Biedl syndrome.Mol Vis2013; 19:644-653.Impact Factor = 1.987
  6. Ahmad W, Whittal RA, Riaz M, Putt W,Ajmal M, Sadeque A, Ayub H, Qamar R, Humphries SE. The genetic spectrum of familial hypercholesterolemia in Pakistan.ClinicaChimicaActa2013; 421:219-225.Impact Factor = 2.850
  7. Khan MI,Ajmal M, Micheal S, Azam M, Hussain A, Shehzad A, Venselaar H, Bokhari H, de Wijs IJ, Hoefsloot LH, Gill M, Waheed NK,Collin RWJ, den Hollander AI,Qamar R,Cremers FPM. Homozygosity mapping identifies genetic defects in four consanguineous families with retinal dystrophy from Pakistan.Clin Genet2013;84 (3):290-293.Impact Factor = 4.247
  8. Ajmal M, Khan MI, Neveling K, Khan YM, Ali SHB, Ahmed W, Iqbal MS, den Hollander AI, Collin RWJ, Qamar R, Cremers FPM. Novel mutations inRDH5cause fundusalbipunctatus in two consanguineous Pakistani families.Mol Vis2012; 18:1558-1571.Impact Factor = 1.987
  9. Ajmal M, Khan MI, Micheal S, Ahmed W, Shah A, Venselaar H, Bokhari H, Azam A, Waheed NK, Collin RWJ, den Hollander AI, Qamar R, Cremers FPM. Identification of recurrent and novel mutations inTULP1in Pakistani families with early onset retinitis pigmentosa.Mol Vis2012; 18:1226-1237.Impact Factor = 1.987
  10. Ahmed W,Ajmal M, Sadeque A, Whittall RA, Rafiq S, Putt W, Khawaja A, Imtiaz F, Ahmed N, Azam M, Humphries SE, Qamar R. Novel and recurrentLDLRgene mutations in Pakistani hypercholesterolemia patients.Mol Biol Rep2012; 39(7):7365-7372.Impact Factor = 2.506
  11. Satti R, Mustafa F, Khan MI, Haq TS, Khan ZU, Zubair M, Rasool STU, Azam M,Ajmal M, Qamar R. Prevalence of Hepatitis C Virus in Urban Ghettos of Twin Cities.Pakistan J Zool2012; 44(4):937-943.Impact Factor = 0.309
  12. Ajmal M, Ahmed W, Akhtar N, Sadeque A, Khalid A, Ali SHB, Ahmed N, Azam M, Qamar R. First report of a nonsense triple nucleotide polymorphism (TNP) in the low density lipoprotein receptor gene in familial hypercholesterolemia.Genet Test Mol Biomarkers2011; 15(9):601-606.Impact Factor = 1.444
  13. Azam M, Collin RWJ, Malik A, Khan MI, Sadeque A, Shah STA, Shah AA, Hussain A,Ajmal M,Arimadyo K, Cremers FPM, Qamar R, den Hollander AI. The genetic basis of retinitis pigmentosa in Pakistan.ArchivOpthalmol2011; 129(10):1377-1378.Impact Factor = 3.826
  14. Ahmed W, Malik M, Khan AA, Saeed I, Sadeque A, Kaleem U,Ajmal M, Azam M, Qamar R. Role of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) polymorphism in Myocardial Infarction.Mol Biol Rep(2011): 38(4):2541-2548.Impact Factor = 2.506
  15. Ajmal M, Ahmed W, Sadeque A, Ali SHB, Bokhari SH, Ahmed N, Qamar R. Identification of a recurrent insertion mutation in theLDLRgene in a Pakistani family with autosomal dominanthypercholesterolemia.Mol Biol Rep(2010).37(8): 3869-3875.Impact Factor = 2.506
  16. Ayub H, Khan MI, Micheal S, Akhtar F,Ajmal M, Shafique S, Ali SHB, den Hollander AI, Ahmed A, Qamar R. Association ofeNOSandHSP70gene polymorphisms with glaucoma in Pakistani cohorts.Mol Vis(2010);16:18-25.Impact Factor = 1.987
  17. Azam M, Collin RWJ, Khan MI, Shah STA, Qureshi N,Ajmal M, den Hollander AI, Qamar R, Cremers FPM. A novel mutation inGRK1causes Oguchi disease in a consanguineous Pakistani family.Mol Vis2009; 15:1788-1793.Impact Factor = 1.987

Total Impact Factor of 16 research papers = 41.045