Liyun LiuCURRICULUM VITAE10/12/06
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Liyun LiuCURRICULUM VITAE10/12/06
PROFESIONAL POSITIONS
2006-present:
Assistant Professor
Department of Biology
CaliforniaStateUniversity
Northridge, California
2004-2006:
Research Associate
Department of Molecular and Cellular Biology
HarvardUniversity
Cambridge, Massachusetts
1999-2004:
Postdoctoral Fellow
Department of Molecular and Cellular Biology
HarvardUniversity
Advisor: Professor J. WOODLAND HASTINGS
Research Area: Biochemistry, protein crystallography and molecular biology of bioluminescence in dinoflagellates
EDUCATION
1991-1999: Ph.D. in Evolutionary and Environmental Biology
Department of Biology, University of Louisiana, Lafayette, Louisiana
Advisor: ProfessorTHOMAS C. PESACRETA
Dissertation Title: Myosin cDNA sequences of corn (Zea mays, Panicoideae): expression in tissues and phylogenetic implications.
1990-1991:Graduate Program in Biology
Department of Biology, University of Maryland, College Park, Maryland
Advisors:Professors STEVEN HUTCHESONand DAVID STRANEY
1986-1990:Doctoral Degree of Natural Sciences
Institute of Botany, Chinese Academy of Sciences, Beijing, China
Advisor: Professor FU-XIONG WANG
Project: Cytological and biochemical Studies of Self-incompatibility in Brassica napus L.
1983-1986:M.S. in Botany,NankaiUniversity, Tianjin, China
1979-1983:B.S. in Biology, Hunan Teacher’s College, Changsha, China
TEACHING EXPERIENCES
Fall semester 2006, Instructor, CaliforniaStateUniversity, Northridge, Courses taught:
BIOL 572: Recombinant DNA Technique
BIOL 572L:Recombinant DNA TechniqueLAB
2000-2005, Department of Molecular and Cellular Biology, HarvardUniversity
Supervision of ten undergraduates in laboratory research
1993-1999, Teaching Assistant, MicroscopyCenter, University of Louisiana, Lafayette
Course taught: Optical and Electron Microscopy in Biological Sciences
RESEARCH EXPERTIES and INTERESTS
2006-present, California State University, Northridge
Genomics and proteomics of dinoflagellatesand malaria parasites
1999-2006, HarvardUniversity
Molecular cloning and bioinformatic analysis of luciferase genes of dinoflagellates
Biochemical characterization and mutagenesis of recombinant dinoflagellate luciferases
Expression of dinoflagellate luciferases in bacteria for X-ray protein crystallography
Crystallization of bacuolovirus-expressed dinoflagellate luciferin-binding protein
Microarray analysis of cell density-dependent gene expression in yeast
Study of functions of the FLT-3 kinase and MAPKAP2 kinase in leukemia
1993-1999, the University of Louisiana
Isolation of myosin genes from corn and onion
Production of plant myosins for generating antibodies in rabbits
Purification of myosins from onion by chromatography
Immunolocalization ofmyosins in plant cells by light and electron microscopy
PUBLICATIONS
1) FULL-LENGTH ARTICLES
11. Liu, L. and Hastings, J.W. Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillons occur as two separate genes in photosynthetic species.Proc. Natl. Acad. Sci. USA (in press).
10. Liu, L. and Hastings, J. W. (2006). Novel and rapidly diverging intergenic sequences between tandem repeats of the luciferase genes in seven dinoflagellate species. J. Phycology42: 96-103.
9. Hastings, J. W., Liu, L., and Schultz, W. (2005). Dinoflagellate bioluminescence and its circadian regulation. InBioluminescence (John Lee, Editor), the Digital Photobiology Compendium (Dennis P. Valenzeno, Editor).
8.Liu, L., Schultz, L. W., and Hastings, J. W. (2005). pH regulation of luciferase activity in dinoflagellates involves a novel enzymatic mechanism. In Bioluminescence & Chemiluminescence: (A. Tsuji, M. Maeda, L.J. Kricka, and P. E. Stanley, Eds.). p.15-19. World Scientific Publ., London.
7. Schultz, L. W., Liu, L., Cegielski, M., and Hastings, J. W. (2005). Crystal structure of a pH-regulated luciferase catalyzing the bioluminescent oxidation of an open tetrapyrrole. Proc. Natl. Acad. Sci. USA 102: 1378-1383.
6. Yang, X., Liu, L., Sterngberg, D., Tang, L., Galinsky, I., DeAngelo, D., and Stone, R. (2005). The FLT3 internal tandem duplication mutation prevents apoptosis in interleukin-3-deprived BaF3 cells due to protein kinase A and ribosomal S6 kinase 1–mediated BAD phosphorylation at Serine 112. Cancer Research 65: 7338-7347.
5. Liu, L., Wilson, T., and Hastings, J. W. (2004). Molecular evolution of dinoflagellate luciferases, enzymes with three catalytic domains in a single polypeptide. Proc. Natl. Acad. Sci. USA101: 16555-16560.
4. Liu, L., Im, H., Cegielski, M., LeMagueres, P., Schultz, L. W., Krause, K. L., and Hastings, J. W. (2003). Characterization and crystallization of active domains of a novel luciferase from a marine dinoflagellate. Acta Crystallogr. D59: 761-764.
3. Okamoto, O. K., Liu, L., Robertson, D. L., and Hastings, J. W. (2001). Members of a dinoflagellate luciferase gene family differ in synonymous substitution rates. Biochemistry 40: 15862-15868.
2. Li, L., Liu, L*., Hong, R., Robertson, D. L., and Hastings, J. W. (2001). N-terminal intramolecularly conserved histidines of three domains in Gonyaulax luciferase are responsible for loss of activity in the alkaline region. Biochemistry 40: 1844-1849.(* joint first author).
1. Liu, L., Zhou, J., and Pesacreta, T. C. (2001). Maize myosins: diversity, localization, and function. Cell Motility and the Cytoskeleton 48: 130-148.
2) ABSTRACTS AND MEETING REPORTS
9. Liu, L. and Hastings J.W. (2004). Phylogenetic analysis of dinoflagellate luciferase genes from seven species: a possible role for conserved nucleotides in the circadian regulation of protein synthesis. Bioluminescence & Chemiluminescence: 12th International Symposium, Yokahama, Japan, supplement.
8. Schultz, W., Cegielski, M., Liu, L., and Hastings, J.W. (2004). Structural studies of a luciferase from the marine dinoflagellate, Lingulodinium polyedrum. Abstracts of the 32nd Annual Meeting of American Society for Photobiology, Seattle, WA.
7. Krause K, Le Mageres1, P., Im, H., Schultz, W., Hastings, J.W., and Liu, L. (2003). Three-dimensional structure analysis of the D2 domain of Lingulodinium luciferase. Abstracts of the 31st Annual Meeting of American Society for Photobiology, Baltimore, MD.
6. Hastings, J.W., Liu, L., Krause, K., Im, H., and Schultz, W. (2002). Structural studies of the catalytic domains from a dinoflagellate luciferase. Abstracts of the 30th Annual Meeting of American Society for Photobiology. Quebec City, Canada.
5. Okamoto O.K., Liu, L.,Robertson, D., and Hastings, J.W. (2001). Molecular structure of the luciferase gene from the dinoflagellate Pyrocystis lunula. Abstracts of the 29th Annual Meeting of American Society for Photobiology, Chicago, IL. p. 34.
4. Liu, L. and Hastings, J.W. (2001). Structural requirements for enzymatic activity in a single catalytic domain of a dinoflagellate luciferase. Abstracts of the 29th Annual Meeting of American Society for Photobiology, Chicago, IL. p. 31.
3. Liu, L., Robertson, D., and Hastings, J.W. (2000). N-terminal histidines are responsible for the decrease in Gonyaulax luciferase activity at pH 8.0. Bioluminescence & Chemiluminescence: 11th International Symposium, Asilomar, CA. p. 60.
2. Liu, L. and Pesacreta, T.C. (1997). Multiple myosins from maize. Plant Physiology 114: 855.
1. Liu, L. and Pesacreta, T.C. (1994).Diversity of monocotyledon myosin genes. Molecular Biology of the Cell (Suppl.) 5: 277a
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