Microelectrode Techniques for Cell Physiology
33rd Workshop Aug 31-13 September 2016
Wednesday 31st 5.45 Registration 6.00 Film ‘The squid and its giant nerve fibre’
Thursday 1 9.00 Introduction David Ogden
11.00 Electronics - The Electronics team
Friday 2 9.00 Voltage Clamp techniques Sandy Harper
10.00 Patch clamp Techniques David Ogden
11.30 Whole cell patch clamp recording Boris Barbour
2.00 Single Electrode Discontinuous Voltage Clamp Alasdair Gibb
3.00 Ion-selective microelectrodes Juha Voipio
4.30 Fluorescent Indicators Martin Thomas
6.00 Transgenic and viral labelling of neurons Jon Bradley
Saturday 3 9.00 Computers and Data Analysis John Dempster
10.30 Software control of patch clamp amplifiers Hubert Affolter
Experiments Block 1 Saturday-Tuesday
6.00 Capacitance measurements Gregor Zupancic
Sunday 4 FREE
Monday 5 9.00 Conventional and Confocal Microscopy Brad Amos
10.30 Microscopy Demonstrations- Brad Amos/ Noah Russell
6.00. Extracellular Recording Clement Lena
Tuesday 6 9.00 Amperometric recording Liz Seward
11.00 Multi-electrode arrays in network analysis Ed Mann
Demonstrations: Amperometry - Liz/Hubert/Tom Carter
6.00 Channel reconstitution and bilayer recording Alan Williams
Evening ‘staff change-over’ Social ALL WELCOME
Wednesday 7 9.00 Permeabilised patch recording Martin Puskarjov
Experiments Block 2 Wednesday-Friday
Demonstration Permeabilised Patch recording
6.00 Microelectrodes as sensors Peter Smith
Thursday 8 9.00 Cell labelling techniques Dave Becker
Possible visit to National Marine Aquarium, back for 1pm lunch
Demonstrations - Multi electrode arrays –Tommas Ellender, Chris Brady
6.00 Electrolyte solutions David Ogden
Friday 9 9.00 Dynamic clamp of membrane conductance Stephen Brickley
Demonstration Dynamic conductance clamp - Stephen Brickley
6.00 Channel evolution and electrical excitability:
from unicells to plants and animals Colin Brownlee
Saturday 10 9.00 Photolysis David Ogden
Experiments Block 3 Saturday-Tuesday
6.00 Membrane voltage and optogenetic probes Thomas Knopfel
Sunday 11 FREE
Monday 12 9.00 Optimising voltage and patch clamp techniques Reiner Polder
Demonstration; Filtering and signal processing - Reiner Polder
Demonstration: Channel Rhodopsin in C elegens – Fernando Calohorro, Patricia Isquierdo
6.00 Analysis of single channel records David Colquhoun
Tuesday 13 9.00 Interpretation of single channel data David Colquhoun
Demonstration: Channel Rhodopsin in C elegens – Fernando, Patricia
6.00 Whole cell recording and transfection in the brain Troy Margrie
Farewell Dinner