KLEU College of Pharmacy, Belagavi
B.Pharm V-Semester (RS2) (2015-16)
Pharmaceutical Biotechnology- Multiple Choice Questions
SI. No. / Content / AnswerUnit- I/ Applied Microbial Technology
1. / Unicellular organism:
A)Yeast
C) Protozoa / B) Bacteria
D) Virus
2 / Viruses are:
A) Parasite
C)Obligate intracellular parasite / B) Saprophite
D) Obligate intracellular saprophite
3 / Alcohol is produced form:
A)Yeast
B)Mold / C)Bacteria
D)Actinomycetes
4 / The Gram stain and acid-fast stain are based on chemical properties of the:
A) Plasma membrane
C)Cell walls / B)Outer membrane
D) Periplasmic space
5 / Primary screening of organic acid and organic amine producing organisms identified by:
A) pH indicating dye
C) Dilution method / B) Crowded plate technique
D) Gradient plate technique
6 / Microorganism isolation is:
A) Purification of culture
C)Separationof asinglecolony / B)Introduction of inoculums
D) To grow microorganisms on surface
7 / Streptomycin is produced from:
A)Streptomyces griseus
C) Streptomyces aureofaciens / B)Micromonospora purpurea
D) Streptomyces erythreus
8 / Pencillin is produced from the organism:
A)Penicillium chrysogenum
C) Penicillium griseofulvin / B) Cephalosporium acrimonium
D) Streptomyces venezuelae
9 / Streptomycin is an:
A)Aminoglycoside
C) Quinolones / B)Beta lactam antibiotics
D) Macrolide
10 / Actinomycetes are:
A)Eukaryotes
B)C) Quinolones / C)Beta lactam antibiotics
D)Macrolide
11 / Thebest mediumfortheproduction of Penicillin is
A) Nutrient agar / B)Cornsteepliquor
C)Sulfitewasteliquor / D) Whey
12 / Penicillin is recovered from the fermentation broth by:
A)Precipitation
C) Chromatography / B)Solvent extraction
D)Adsorption on charcoal
13 / Riboflavin is produced from:
A)Clostridium butyricum
C) Saccharomyces cerevisae / B)Acetobacter suboxydans D)Torula lactosa
14 / Penicillin is discovered by:
A)Robert koch
C) Louis pastuer / B)Edward jenner
D)Alexander fleming’s
15 / Vitamin c is produced by fermentation using the microorganism:
A)Acetobacter suboxydans
C) Saccharomyces pombe / B)Aerobacter aerogenes
D) Saccharomyces cerevisiae
16 / Penicillin is sterilized by:
A)Moist heat
C) Autoclave / B)Dry heat
D) Chemical agent
17 / Streptomycin is produced from:
A)Molds
C) Virus / B)Protozoa
D) Actinomycetes
18 / Industrial production of streptomycin involves:
A)Anaerobic submerged technique
C) Anaerobic Surface culture / B)Aerobic submerged technique
D) Aerobic surface culture
19 / Highest yield of streptomycin requires:
A)Nitrogen source
C) Carbon source / B)Meat extract
D) Minerals
20 / Proteolytic activity of Streptomyces griseous releases :
A)Ammonia
C) Calcium / B)Carbon
D) Glucose
21 / Streptomycin is available as:
A)Sulphate form / B) Hydrochloride form
C) Sodium salt form / D) Base form
22 / Industrial production of streptomycin involves:
A)Anaerobic submerged technique / B)Aerobic submerged technique
C) Anaerobic Surface culture / D) Aerobic surface culture
23 / Enzymes responsible for alcoholic fermentation:
A)Ketolase / B) Zymase
C) Peroxidase / D) Oxidase
24 / Which type of spores are produced sexually?:
A)Conidiospores / B) Sporangiospores
C) Ascospores / D) Chalmidospores
25 / In the citric acid production, the pH to be maintained in the fermentor is:
A) 3.5 / B)5.0 to 6.0
C) 8.0 to 9.0 / D) 1.0 to 6.0
26 / Cobalt is added as precursor in the fermentation of :
A)Vitamin B 12 / B) Vitamin C
C) Vitamin B 6 / D) Vitamin D
27 / The required temperature for the production of citric acid is:
A) 10oC – 80oC / B) 30oC – 50oC
C) 20oC – 50oC / D) 25oC – 30oC
28 / Theby-productduringstreptomycin production is:
A) VitaminA / B)Vitamin B2
C)Proline / D) VitaminB 12
29 / β-lactum ring is present in:
A) Erythromycin Diskplate method / B) Streptomycin
C)Penicillin / D) Tetracyclines
30 / Aspergillus niger is used generally for the production of:
A) Ethanol / B) Penicillin
C) Citric acid / D) Lactic acid
31 / The raw material for citric acid production is:
A) Corn / B) Molasses
C) Starch / D) Whey
32 / Citric acid is produced in aerobic conditions by the fungi:
A) Aspergillus niger / B) Saccharomyces
C) Agaricus bisporus / D) Chrysogenum notatum
33 / Phages are:
A) Bactria / B) Fungi
C) Protozoa / D) Virus
34 / Citric acid is used as:
A) Acidulant / B) As an antioxidant
C) As preservative / D) Pickling agent
35 / In the production of ethanol industrially the yeast used is:
A) K.pneumoniae / B) Kluyreromyces fragilis
C) S. cerevisiae / D) Aspergillus niger
36 / Clostridium acetobutylicum is used for the production of:
A) Acetone – Butanol / B) Ethanol
C) Vitamin-B12 / D) citric acid
37 / Citric acid is used as:
A) Flavoring agent in food / B) As an antioxidant
C) As preservative / D) Pickling agent
38 / The most common substrate used in distilleries for the production of ethanol is:
A) Molasses / B)Corn meal
C) Soya meal / D) Ground gram
39 / Industrial Production of Vitamin-B12 is from:
A) Propionibacterium sps. / B)Pseudomonas sps.
C)Pseudomonas sps.+ Propionibacterium sps. / D) Candida sps
40 / The penicillin produced in large scale submerged fermentation is:
A) Penicillin A / B)Penicillin G
C) Penicillin D / D) PAP
41 / The pH to be maintained for the production of penicillin is:
A) 7.5 / B)6.5
C) 8.0 / D) 5.0
Unit _II / Enzyme Technology
42 / Support material used for immobilization of enzyme by adsorption method:
A) Cellulose / B) Gelatin
C) Alginate / D)Glutaraldehyde
43 / Advantage of immobilization of enzyme:
A) High rate of reaction / B) less stable
C) Continuous use / D)Low rate of reaction
44 / Medium containing all nutrient s in excess but controlling one nutrient is referred as :
A) Turbidostat / B) Chemostat
C) Continuous culture / D) batch culture
45 / Methodsusedtogetimmobilizedenzymes:
A) Adsorption / B)Absorption
C)Chemical reaction / D) Ion exchange
Unit –III/Bioprocess Technology
46 / First Pasteur conducted fermentation experiments in:
A) Milk / C) Fruit juices
C) Fruit juices / D) Milk and fruit juices
47 / Fixed bed fermentor is also known as:
A) Immobilized cell fermentor / B) Fed batch fermentor
C) Tower fermentor / D) Cyclone column fermentor
48 / Tower fermentors are used for the production of:
A) Enzymes / B) Beer
C) Antibiotic / D) Vitamins
49 / Downstream processing involves:
A) Purification / B) extraction
C) Production / D) extraction and purification
50 / Upstream processing involves:
A) Development & production / B) medium selection
C) Production / D) recovery of product
51 / The capacity of laboratory fermentor is:
A) 12-15 lt / B)2 lt
C) 500 lt / D) 10 lt
52 / The overheating of fermentor during fermentation is controlled by
A) Cooling agent / B)Steam
C) Cool air / D) Air bubbling
53 / In fermentor the top portion left without broth is called:
A) Shaft / B )Impeller
C) Sparger / D) Head space
54 / The purification and recovery of product after fermentation is called:
A) Upstream processing / B)Downstream processing
C) Surface fermentation / D) Submerged fermentation
55 / Production of desirable product in the fermentor is called:
A) Upstream processing / B)Downstream processing
C) Surface fermentation / D) Submerged fermentation
56 / Batch fermentation is also called:
A) Open system / B)Fed batch
C) Closed system / D) Continuous fed batch
57 / Antifoam agent is
A) Silicon compound / B) Corn oil
B)Soyabean oil / C)All the above
Unit- IV/Genetic Engineering
58 / Restriction endonuclease is employed for cutting:
A) A single stranded DNA / B) Double stranded DNA
C) RNA fragment Anaerobic Surface culture / D) mRNA culture
59 / Restriction endonucleases are:
A) Used in genetic engineering for uniting two DNA molecules / B) Used for in vitro DNA synthesis
C) Present in mammalian cells for degeneration of DNA of dead cells / D) Synthesized by bacteria for their defense
60 / Structure of DNA was given:
A) Kornberg / B) Nirenberg
C) Watson and Crick / D) Holley and Nirenberg
61 / The basic unit of a nucleic acid is
A) Pentose sugar / B) Nucleoid
C) Nucleoside / D) Nucleotide
62 / The enzyme taking part in joining two ends of DNA is:
A) Ligase / B) Polymerase
C) Gyrase / D) Helicase
63 / In callus culture, roots can be induced by the supply of:
A) Auxin and no cytokinin / B) Higher concentration of auxin and lower concentration of cytokinin
C) Higher concentration of cytokinin and lower concentration of auxin / D) Both auxin and cytokinin in equal proportions
64 / In double helix of DNA, the two DNA strands are:
A) Coiled around a common axis / B) Coiled around each other
C) Coiled differently / D) Coiled over protein sheath
65 / It is now possible to breed plants and animals with desired characters through:
A) Genetic engineering / B) Chromosome engineering
C) Ikebana technique / D) Bonsai technique
66 / Plasmids are vectors for gene cloning because they
A) Replicate freely outside bacterial cells / B) Self replicate in bacterial cells
C) Can be multiplied in cultures / D) Can be multiplied in laboratories using enzymes
67 / DNA is composed of repeating units:
A) Ribonucleosides / B) Deoxyribonucleosides
C) Ribonucleotides / D)Deoxyribonucleotides
68 / Thegene transfer occurs by:
A) Transformation / B) Transduction
C) Conjugation / D) Cellfusion
69 / Gene is segment of:
A) RNA / B) DNA
C) RNA or DNA / D) Both DNA and RNA
70 / Bacterial plasmid contains:
A) RNA / B) RNA + protein
C) DNA / D) Photosynthetic structures
71 / Choose the correct statement:
A) DNA is hereditary material / B) RNA is hereditary material
C) DNA is hereditary material but where it is absent RNA can function as hereditary material / D) Both DNA and RNA are hereditary materials
72 / In recombinant DNA technology a plasmid vector is cleaved by:
A) Modified DNA ligase / B) A heated alkaline solution
C) The same enzyme that cleave the donor DNA / D) The different enzyme other than that cleave the donor DNA
73 / The most common plasmid vector used in genetic engineering is:
A) PBR 328 / B) PBR 322
C) PBR 325 / D) PBR 330
74 / Eco RI is an
A) Ligase / B) Polymerase
C) Restriction enzyme / D) Gyrase
75 / Extranuclear genetic material is found in:
A) Plastid and nucleus / B) Mitochondria and plastids
C) Nucleus and cytoplasm / D) Mitochondria and nucleus
76 / The molecular formulae of deoxyribose sugar and ribose sugar respectively are:
A) C5 H10 O4 and C5H10O6 / B) C5 H10 O4 and C5H10O5
C) C5 H10 O5 and C5H10O4 / D) C5 H10 O5 and C6H10O4
77 / DNA differs from RNA in:
A) Presence of deoxyribose sugar / B) Presence of thymine base
C) Property of replication / D) All the above
78 / RNA contains:
A) Hexose / C) Fructose
B) Ribose / D) Glucose
79 / Similarity between DNA and RNA is that both have:
A) Similar sugars / C) Similar pyrimidines
B) Similar mode of replication / D) Polymers of nucleotides
80 / A small, circular DNA molecule used as a vector to transmit foreign DNA is :
A) Plasmid / B) Liposome
C) Ring chromosome / D) Primer
81 / The first drug produced using recombinant DNA technology was:
A) Streptokinase / B) Insulin
C) Erythropietin / D) Human growth hormone
82 / The polymerase chain reaction is used to:
A) Create millions of copies of an interesting piece of DNA / B) Make more copies of DNA polymerase
C) Speed the rate of DNA replication in cells / D) Copy protein into RNA
83 / Genetic engineering manipulates gene products at the level of:
A) The protein / B) Amino acids
C) DNA / D) RNA
84 / A molecule that consists of a piece of DNA from one organism combined with the DNA from a member of another species is called:
A) Transgenic DNA / B) Bioengineered DNA
C) Recombinant DNA / D) Restricted DNA
85 / The functions of plasmid are:
A) DNA replication / B) Protein synthesis
B) Protein synthesis / D) Genetic engineering
86 / Annealing in genetic engineering is:
A) Joining of DNA / B) Cutting of DNA
C) Joining of RNA / D) Slicing of RNA
87 / Which of the following bacteria is not a source of Restriction endonuclease
A)Haemophilus influenzae / B)Escherichia coli
C)Agarobacterium tumefaciens / D)Bacillus amyloli
88 / The first step in polymerase chain reaction (PCR) is
A) Denaturation / B) Primer extension
D)Annealing / E)Cooling
89 / What is another name for a restriction enzyme?:
A) Vector / B)Plasmid
C)Restriction endonucleases / D) Restriction sequence
Unit-V/Biologicals and Biopharmaceuticals
90 / In tissue / bacterial culture nutrients are sterilized through:
A) Water bath at 200° C / B) Dry air oven at 200° C
C) Dehumidifier / D) Autoclave
91 / In tissue culture, callus can be induced to form shoot or root by altering the ratio of:
A) Auxin to cytokinin / B) Cytokinin to ethylene
C) Auxin to gibberellin / D) Gibberellin to cytokinin
92 / Genetically engineered bacteria are being used in commercial production of:
A) Melatonin / B) Testosterone
C) Human insulin / D) Thyroxin
93 / A totipotent cell means:
A) An undifferentiated cell capable of developing into a system or entire plant / B) An undifferentiated cell capable of developing into an organ Testosterone
C) An undifferentiated cell capable of developing into complete embryo / D) Cell which lacks thecapability differentiate into an organ or system
94 / Callus is
A) Tissue that forms embryo / B) An insoluble carbohydrate
C) Tissue that grows to form embryoid / D) Unorganized actively dividing mass of cells
95 / Development of shoot and root in tissue culture is determined by:
A) Cytokinin and auxin / B) Enzymes
C) Temperature / D) Plant nutrients
96 / Raising of plants from a small tissue in culture is known as:
A) Macro production / C) Tissue culture
B) Micro propagation / D) Mass production
97 / Which of the following is not tool of genetic engineering?:
A) Vectors / C) Foreign DNA
B) Enzymes / D) GMO
98 / Industrially important Antibiotic producing organisms shall be isolated by:
A) Diskplate method / C) Directplatemethod
B) Serialdilutionmethod / D) Crowded platemethod
99 / Vaccines are preparedfrom killed microbes, they are
A) Inactivated(killed)vaccine / C) Autogenous vaccine
B) Attenuatedvaccines / D)Toxoids
100 / Vaccines prepared from toxins and chemicals are:
A) Cellular vaccines / C) Attenuatedvaccines
B) Sub-cellular vaccines / D) Heterologous vaccines
101 / Hybridoma technique was first discovered by:
A) Kohler and Milstein / C) Herelle
B) Robert Koch / D) Land Steiner
102 / The capacity of a given strain of microbial species to produce disease is known as:
A) Pathogen / C) Infection
B) Virulence / D) Disease
103 / Term vaccine was coined by:
A)Robert Koch / B)Needham
B) Pasteur / D) Land Steiner
104 / The bacterial cells are at their metabolic peak during:
A) Lag phase / C) Stationary
B) Log / D) Decline
105 / Toxins or enzymes which are not produced by streptococcus pyrogens:
A) Hyaluronidase / C) Hemolysin
B) Phosphate / D) Streptokinase
106 / Attenuation means:
A) Killing of the bacteria (microorganism) / C) Inactivation of bacteria
B) More activating the bacteria / D) killing and inactivation of bacteria
107 / The method used to maintain a continuous culture:
A) Chemostat / C) Thermostat
B) Autostat / D) Chemostat and thermostat
108 / The organisms which can grow both in presence and absence of oxygen:
A) Aerobes / C) Faculative anaerobes
B) Anaerobes / D) Strict aerobes
109 / In which medium the hydridoma cells grow selectively?:
A)Polyethylene glycol / C) Hypoxanthine aminopterin thyminine
B) phosphoribosyltransferase / D) Molasses
110 / The enzymes which are commonly usedin genetic engineering are:
A) Exonuclease and ligase / C) Ligase and polymerase
B) Restriction endonuclease and polymerase / D) Restriction endonuclease and ligase
111 / A successful hybridoma was produced by fusing:
A) Plasma cells and plasmids / C) Myeloma cells and plasmids
B) Plasma cells and myeloma cells / D) Plasma cells and bacterial cell
112 / Plasmids are ideal vectors for gene cloning as:
A) They can be multiplied by culturing Hyalauronidase / C) They can replicate freely outside the bacterial cell
B) They can be multiplied in the laboratory using enzymes / D) They are self replicating within the bacterial cell of the DNA
113 / Self-replicating, small circular DNA molecules present in bacterial cell areknown:
A) Plasmids / C) Plasmomeros
B) Cosmids / D) Plastids
114 / Monoclonal antibodies are produced by:
A) Hybridoma technology / C) Fermentation Technology
B) Biotechnology / D) Biosynthesis
115 / Enzymes are chemically:
A) Lipids / C) Carbohydrates
B) Proteins / D) Fats
116 / Hybridoma technique is used for:
A) Monoclonal antibodies / C) Proteins
B) Polyclonal antibodies / D) Antigens
117 / Phyto-pharmaceuticals are produced from:
A) Bacteria / C) Plants
B) Fungi / D) Animals
118 / Protoplast fusion involves:
A) Fusion of DNA / C) Fusion of cells without cell wall
B) Fusion of nucleus / D) Fusion of Cells with cell wall
119 / Enzymes used in the Protoplast isolation:
A) Pectinase and Cellulase / C) Amylase and pectinase
B) Catalase and Lipase / D) Zymase and Amylase
120 / Bio-pharmaceuticlas are:
A) Proteins or nucleic acids / C) Amino acids or antibiotics
B) Lipids or carbohydrates / D) Amino acids or fats
121 / Biopharmaceuticals currently produced by genetic engineering in either:
A) E coli or animal cell / C) Virus or animal cell
B) Plant or animal cell / D) E coli or virus
122 / Erythropoietin is secreted by:
A) Cells in macula densa / C)Juxtaglomerularcells
B)Cells in the proximal tubules / D) Cells in peritubular capillary bed
Unit- VI/Medicinal Plant Biotechnology and Animal Cell Technology
123 / The enzymes required to obtain wall-free / naked protoplasts are:
A) Cellulase and proteinse / B)Cellulase and Pectinase
C) Cellulase and Amylase / D) Amylase and Pectinase
124 / Polyethylene glycol is:
A) Fusogenic material / B)Electro fusion stimulant
C) Callus stimulant / D) differentiation stimulant
125 / Plant tissue culture technique is a redefined method of:
A) Vegetative propagation / B)Asexual reproduction
C) Hybridization / D)Selection
126 / Somatic hybridization is achieved through:
A) Grafting / B)Protoplast fusion
C) Conjugation / D) Recombinant DNA technology
127 / The first transgenic crop was:
A) Pea / B)Tobacco
C) Flax / D) Cotton
128 / The technique of hybridoma formation is from:
A) Spleen cell – Myeloma cell / B)Spleen cell – Spleen cell
C) Myeloma cell – Myeloma cell / D) kidney cell - Myeloma cell
129 / The transgenic animals are those which have:
A) Foreign DNA in some of their cells / B)Foreign DNA in all of
C) Foreign RNA in all of their cells / D) Foreign RNA in some of their cells
130 / Polyethylene glycol method is used for
A) Energy production from sewage / B)Gene transfer without a vector
C) Seedless fruit production / D) Biodiesel production
131 / Which part would be most suitable for raising virus free plants for micropropagation
A)Meristem / B)Node
C)Bark / D)Vascular tissues
132 / Transgenic animals are for the improvement in quality of
A)Milk / B)Meat
C)Egg / D)All the above
133 / The first crop plant sequenced is,
A) Rice / B)Tobacco
C) Maize / D) Cotton
134 / DNA finger printing was developed by
A)Francis Crick / B)Khorana
C)Alec Jeffery / D)James Watson
Unit-VII /Proteomics and Genomics
135 / The proteome is
A) only useful to be studied in conjunction with the phenomena / B)refers to the entire complement of proteins
C) is what functional genomics is primarily interested in understanding / D) is now most commonly studied using RNA microarrays
136 / A Map Unit refers to
A) the relative distance between genes on a chromosome / B) the chromosomes that exchange parts during meiosis
C) the percentage of recombination / D) A and C
137 / Genetics is the science of
A)Genes / B)Heredity
C) the variation of organisms / D) genes, heredity & the variation of organisms
138 / Genomics is the study of
A)genetic patterns of genome of species / B)genes
C)genes synthesizing proteins / D)genes as diagnostic tools
E)
139 / Proteomics is the study of
A)codons / B)proteins
C)proteins and genes / D)genes
140 / Human genome project was completed in
A)January 2003 / B)April 2013
C)April 2003 / D)January 2013
141 / Proteomics was coined by
A)Robert Koch / B)Marc wilkins
C)Thomas Rederick / D)Elizabeth Bugie
142 / Genomics was coined by
A)Marc wilkins / B)Alexander Fleming
C)Thomas Rederick / D)Albert Schatz
Unit-VIII / Nanotechnology and Nanomedicine
143 / Who coined the word 'nanotechnology'?
A) Eric Drexler / B) Richard Feynmann
C) Sumio Tijima / D) Richard Smalley
144 / Which formulation contains nanoparticles prepared by using biologically processed metal ores.
A)Homeopathic medicines / B) Modern antibiotics
C)Ayurvedic 'Bhasmas' / D)Modern cosmetics
145 / The processing of separation, consolidation and deformation of materials by one atom or one molecule is called as:
A) Biotechnology. / B) Physics.
C) Nanobiotechnology. / D)Chemistry.
146 / Which is the surface science and physical chemistry that focuses on fabrication of structures in carbon, silicon or other materials?
A) Wet nanobiotechnology / B) Computational nanobiotechnology
C) Drug nanobiotechnology / D) Nan biotechnology
147 / Which metal is used with nanoparticles for antibiotic delivery?
A) Gold / B) Titanium
C) Zinc / D) Silver
148 / Nano shells are used in the treatment of which of the following disease?
A) Alzheimer’s / B) Cancer
C) HIV / D)Parkinson’s
149 / A Nano biological recognition component, which is involved in interacting with the analyte molecule is called as:
A) Biosensor / B) Probe
C) Nanobiosensor / D) Quantum Dots
150 / Tiny semiconductor Nanoparticles with fascinating light-emitting properties are called as:
A) Nanoparticles / B) Nanopores
C) Bucky Balls / D) Quantum Dots