Jordan University of Science and Technology

Faculty of Science and Arts

Department of Chemistry

Second Semester 2007

Course Syllabus

Course Information
Course Title / Advanced Chromatographic Methods of Analysis
Course Code / CHEM 732
Prerequisites
Course Website
Instructor / Dr. Yahya R. Tahboub
Office Location / D3L0
Office Phone # / 23588
Office Hours / Sun, Tue, Th 8-10 am, Mon, Wed 11 am-1 pm
E-mail /
Teaching Assistant(s)
Course Description
The ultimate goal of this course is to give the student the skills required for method development employing popular chromatographic techniques. The course will cover in depth separation equilibria and operational aspects of separation. Problem solving employing gas chromatography and liquid chromatography. Employing mass spectrometry in identification and detection of separating analytes. Environmental and biomedical applications
Textbook
Title / The Essence of Chromatography
Author(s) / C. F. Pool
Publisher / Elsevier
Year / 2003
Edition
Book Website
Other references
Assessment
Assessment / Expected Due Date / Percentage
First Exam / 2nd week of March / 20
Second Exam / 4th week in April / 20
Final Exam / 1st week of June / 40
Assignments / Project on Applications of Chromatography / 20
Participation
Attendance
Course Objectives / Percentage
1.  Separation Equilibria / 15%
2.  Operational Aspects of Separation / 15%
3.  Gas Chromatography: Principles, Instrumentation and Applications / 20%
4.  Liquid Chromatography: Principles, Instrumentation and Applications / 20%
5.  Mass Spectrometry as Universal Detector in Chromatography / 10%
6.  Applications
Teaching & Learning Methods
Class periods will be a mixture of theory, analysis, demonstration, and discussion. I believe in the active-learner approach. You are required to read.
Learning Outcomes: Upon successful completion of this course, students will be able to
Related Objective(s) / Reference(s)
1 / Have knowledge of life inside the chromatographic column including theoretical treatment / Pool, Ch 2-3, Handouts
2 / Have deep knowledge of GC and solving analytical problems involving GC / Pool, Ch 4, Handouts
3 / Have deep knowledge of LC and solving analytical problems involving LC / Pool, Ch. 5, Handouts
4 / Have knowledge of theory and instrumentation of mass spectrometry and its employment in chromatographic detection including MALDI-TOF / Handouts
1-4 / Method development employing chromatographic separations especially with mass spectrometric detection / Handouts
Useful Resources
1.  L. R. Snyder, J. J. Kirkland and J. L. Glajch, Practical HPLC Development, Wiley 1997.
2.  H. J. McNair and J. M. Miller, Basic Gas Chromatography, Wiley 1998.
3.  J. H. Gross, Mass Spectrometry: A Text Book, Springer-Verlag 2004
4.  J and M. Rose, Mass Spectrometry for Chemists and Biochemists, Cambridge University Press 1996.
5.  C. Dass Practice of Biological Mass Spectrometry, Wiley 2001.
Course Content
Week / Topics / Chapter in Textbook (handouts)
1 / Validation of Analytical Methods / Handout
2-3 / Separation Equilibria: Thermodynamics of separation, auxiliary equilibria, acid-base equilibria, complexation equilibria., kinetics of separation / Pool, Ch 2, Handouts
4-6 / Operational Aspects of Separation: Methods of separation, chromatographic separations, resolution, evaluation of needed performance to achieve separation (band broadening), multi-component separations / Pool, Ch 3, Handouts
7-8 / The Separation Process in Gas Chromatography, Injection Methods and Detection Methods / Pool, Ch 4, Handouts
9-10 / The Chromatographic Process in Liquid Chromatography. Injection and Detection in Liquid Chromatography, / Harris, Ch 25, Handouts
10 / Principles of Mass Spectrometry and Fragmentation Process / Handouts
12 / Type of Mass Spectrometers / Handouts
13 / Gas Chromatography-Mass Spectrometry / Handouts
14 / Liquid Chromatography-Mass Spectrometry / Handouts
15 / Method Development Employing Gas Chromatography / Handouts
16 / Method Development Employing liquid Chromatography / Handouts
Assignments / A set of problems will be assigned at somewhat irregular intervals but will always be due before class on each due date. Policy on late homework: late homework will not be accepted without a serious or compelling reason.
Exams / There will be two exams and a comprehensive final. If an exam is missed for a documented serious and compelling reason (e.g., signed doctor's excuse), a make-up exam will be held at a time and place of the instructor's discretion. Equation sheets will be provided during the exams. Please bring only pencils and calculators to the exams.
Tentative Exam Schedule:
1. Two Majors (Class Tests): 2 x 20% = 40%
I. First Exam: Tuesday March (2nd week), 2007.
II. Second Exam: Tuesday April (4th week), 2007.
III. Project 20%
2. Final Exam: May 26-June 4, 2007 40%
TOTAL 100%
Academic dishonesty of any form will not be tolerated. University policies on cheating and plagiarism (see Students' Guide) will be strictly enforced.
Attendance / You are required to attend all lectures. Please see me if you have an extended illness or family emergency. You must initial the attendance sheet on the side blackboard every day so that I have a quick check of who is missing.
Workload / …………….
Graded Exams / …………….
Participation / No points, but students are highly encouraged to participate in the discussion.
Laboratory / None
Projects / None
Disclaimer / The instructor reserves the right to make changes to this course and its administration as reasonable and necessary.