Supplementary material

Aryl hydrocarbon receptor (AhR) inducers and estrogen receptor (ER) activities in surface sediments of Three Gorges Reservoir, China evaluated with in vitro cell bioassays

Jingxian Wang a,b,c,*, Toine F.H. Bovee d, Yonghong Bi c, Silke Bernhöft b, Karl-Werner Schramm a,b*

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Table S1 Concentrationa and the Calculated TEQs for PCDD/Fs, PCBs and PAHs in sediments from Three Gorges Reservoir

Chemical / TEF/REPc / WZ / MP / WS / BD / GJB
2378-TCDD / 1 / 0.03 / n.d.b / 0.03 / 0.08 / 0.16
12378-PeCDD / 1 / 0.10 / 0.08 / 0.11 / 0.15 / 0.1
123478-HxCDD / 0.1 / 0.09 / 0.11 / 0.20 / 0.27 / 0.12
123678-HxCDD / 0.1 / 0.10 / 0.16 / 0.16 / 0.24 / 0.14
123789-HxCDD / 0.1 / 0.09 / 0.09 / 0.27 / 0.24 / 0.21
1234678-HpCDD / 0.01 / 1.19 / 1.5 / 1.85 / 2.06 / 1.8
OCDD / 0.0001 / 20.51 / 39 / 47.26 / 54.00 / 44.6
2378-TCDF / 0.1 / 0.26 / 0.2 / 0.34 / 0.59 / 0.28
12378-PeCDF / 0.05 / 0.16 / 0.18 / 0.32 / 0.28 / 0.3
23478-PeCDF / 0.5 / 0.22 / 0.16 / 0.25 / 0.33 / 0.2
123478-HxCDF / 0.1 / 0.32 / 0.46 / 0.30 / 0.61 / 0.37
123678-HxCDF / 0.1 / 0.31 / 0.4 / 0.22 / 0.50 / 0.29
123789-HxCDF / 0.1 / 0.05 / 0.11 / 0.16 / 0.16 / 0.11
234678-HxCDF / 0.1 / 0.26 / 0.39 / 0.30 / 0.55 / 0.34
1234678-HpCDF / 0.01 / 1.83 / 2.1 / 1.87 / 3.04 / 1.5
1234789-HpCDF / 0.01 / 0.27 / 0.3 / 0.23 / 0.32 / 0.25
OCDF / 0.0001 / 4.48 / 4.8 / 7.17 / 9.08 / 6.6
TEQPCDD/F / 0.43 / 0.41 / 0.53 / 0.79 / 0.6
PCB-77 / 0.0001 / 5.6 / 2.6 / 2.5 / n.d. / 4.6
PCB-81 / 0.0001 / n.d. / 0.18 / n.d. / n.d. / 0.2
PCB-126 / 0.1 / n.d. / 0.15 / n.d. / n.d. / 0.26
PCB-169 / 0.01 / n.d. / 0.13 / n.d. / n.d. / 0.21
PCB-105 / 0.0001 / 5.1 / 3.6 / 3.4 / 5.1 / 13.8
PCB-114 / 0.0005 / 0.6 / 0.48 / 0.4 / 0.6 / 1.5
PCB-118 / 0.0001 / 11.5 / 6.7 / 6.5 / 8.1 / 26.9
PCB-123 / 0.0001 / 1.2 / 0.47 / 0.7 / 1.0 / 2.6
PCB-156 / 0.0005 / 1.6 / 0.96 / 0.7 / 1.0 / 5.7
PCB-157 / 0.0005 / 0.6 / n.d. / 0.3 / 0.5 / 2
PCB-167 / 0.00001 / 0.9 / 0.47 / 0.4 / 0.7 / 1.9
PCB-189 / 0.0001 / 0.5 / n.d. / 0.2 / 0.3 / 0.64
TEQPCB / 0.004 / 0.02 / 0.002 / 0.002 / 0.04
B[a]A / 1.4E-06 / 19.6 / 14.4 / 33.3 / 25.9 / 24.3
Chry / 1.6E-06 / 30.2 / 22.9 / 49.1 / 37.1 / 40.6
B[b]F / 0.000004 / 51.6 / 34.2 / 37.7 / 30.7 / 22.3
B[k]F / 0.00011 / 11.5 / 7.9 / 29.7 / 22 / 11.9
B[a]P / 1.3E-06 / 23.1 / 13.9 / 35.9 / 23.5 / 22.9
I[cd]P / 0.000013 / 19.1 / 12.3 / 25.3 / 17.5 / 17.9
D[ah]A / 0.000004 / 6.5 / 4.1 / 9.2 / 5.2 / 7.2
TEQPAH / 1.85 / 1.26 / 3.96 / 2.92 / 1.79
Chem-TEQd / 2.28 / 1.69 / 4.49 / 3.71 / 2.43

a Concentration is expressed as ng/g dw for PAH; pg/g dw for PCDD/Fs, PCBs, and TEQs.

b n.d. means not detected and was not included in the calculation of TEQ.

c TEF/REP values were obtained from references Van den Berg et al., 1998; Villeneuve et al., 2002; Nakata et al., 2003; Qiao et al., 2006.

d Chem-TEQ is the sum of the TEQPCDD/F, TEQPCB, and TEQPAH.

Fig. S1 Dose-response of micro-EROD assay with H4IIE rat hepatoma cells (72 hours incubation) to 2,3,7,8-TCDD standards.

Fig. S2 Dose-response of micro-EROD assay with H4IIE rat hepatoma cells (72 hours incubation) to sediment extracts.

References

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