Jie Cui Ming Li Lin Qiu Jiashu Cao Li Huang

Stable expression of exogenous imported sporamin in transgenic Chinese cabbage enhances resistance against insects

Plant Growth Regulation

Jie Cui﹒Ming Li﹒Lin Qiu﹒Jiashu Cao﹒Li Huang

Author for correspondence,Li Huang, Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China.e-mail:

Supplementary Table 1 Segregation analysis of the PCR-tested T1 progeny population of transgenic Chinese cabbage ‘Youdonger’ (Brassica campestris ssp. chinensis var. communis cv. Youdonger).

Line / Number of positive individual / Number of negative individual / Segregation ratio
(P:N) / Segregation ratio tested / χ2 Value / P-Value
T1-2 / 23 / 7 / 3.3 : 1 / 3 : 1 / 0.023 / 0.878
T1-3 / 25 / 8 / 3.1 : 1 / 3 : 1 / 0.005 / 0.943
T1-4 / 19 / 7 / 2.7 : 1 / 3 : 1 / 0.028 / 0.868
T1-10 / 26 / 9 / 2.9 : 1 / 3 : 1 / 0.05 / 0.946
T1-13 / 20 / 6 / 3.3 : 1 / 3 : 1 / 0.029 / 0.865

All χ2 Value indicate significant fit to the tested ratio (P=0.05).

Supplementary Table 2 Segregation analysis of the PCR-tested T1 progeny population of transgenic Chinese cabbage ‘Shanghaiqing’ (Brassica campestris ssp. chinensis var. communis cv. Shanghaiqing).

Line / Number of positive individual / Number of negative individual / Segregationratio
(P:N) / Segregation ratio tested / χ2 Value / P-Value
T1-2 / 21 / 8 / 2.6 : 1 / 3 : 1 / 0.053 / 0.819
T1-4 / 24 / 8 / 3.0 : 1 / 3 : 1 / 0 / 1
T1-5 / 25 / 9 / 2.8 : 1 / 3 : 1 / 0.019 / 0.891
T1-9 / 24 / 7 / 3.4 : 1 / 3 : 1 / 0.051 / 0.822

All χ2 Value indicate significant fit to the tested ratio (P=0.05).

Supplementary Table 3 Segregation analysis of the PCR-tested T2 progeny population of transgenic Chinese cabbage ‘Youdonger’ (Brassica campestris ssp. chinensis var. communis cv. Youdonger).

Line / Number of positive individual / Number of negative individual / Segregation ratio
(P:N) / Segregation ratio tested / χ2 Value / P-Value
T2-2-1 / 19 / 7 / 2.7 : 1 / 3 : 1 / 0.028 / 0.868
T2-2-2 / 28 / 0
T2-2-3 / 17 / 6 / 2.8 : 1 / 3 : 1 / 0.008 / 0.927
T2-2-4 / 24 / 9 / 2.8 : 1 / 3 : 1 / 0.043 / 0.835
T2-2-5 / 32 / 0
T2-2-6 / 19 / 6 / 3.2 : 1 / 3 : 1 / 0.008 / 0.931

All χ2 Value indicate significant fit to the tested ratio (P=0.05).

Supplementary Table 4 Segregation analysis of the PCR-tested T2 progeny population of transgenic Chinese cabbage ‘Shanghaiqing’ (Brassica campestris ssp. chinensis var. communis cv. Shanghaiqing).

Line / Number of positive individual / Number of negative individual / Segregation ratio
(P:N) / Segregation ratio tested / χ2 Value / P-Value
T2-9-1 / 20 / 7 / 2.9: 1 / 3 : 1 / 0.007 / 0.935
T2-9-2 / 23 / 7 / 3.3: 1 / 3 : 1 / 0.023 / 0.878
T2-9-3 / 19 / 6 / 3.2: 1 / 3 : 1 / 0.008 / 0.931
T2-9-4 / 31 / 0
T2-9-5 / 25 / 8 / 3.1: 1 / 3 : 1 / 0.005 / 0.943
T2-9-6 / 23 / 8 / 2.9: 1 / 3 : 1 / 0.005 / 0.941

All χ2 Value indicate significant fit to the tested ratio (P=0.05).

Supplementary Fig. 1 Transgenic Chinese cabbages(Brassica campestrisssp.chinensis) with Hyg resistance were obtained by Agrobacterium tumefaciens mediated method. A, Sowing Seeds; B,Seedling for 4-5 d; C and D, Cotyledon-hypocotyl explants during pre-culture; E, Co-culture; F, differentiating culture; G-H, Resistant adventitiousbuds or plants of ‘Youdong’er’ and ‘Shanghaiqing’ respectively.

Supplementary Fig. 2 Segregation analysis of T1 progeny population derived from transgenic Chinese cabbage‘Youdonger’ (Brassica campestris ssp. chinensis var. communis cv. Youdonger) line T0-2 inserted by sporamin. M: molecular marker, P+: positive control pCAMBIA1300PMSN-PMCN plasmid, WT: wild type plants, 1-30: T1 progeny plants of line T0-2.

Supplementary Fig. 3 Segregation analysis of T1 progeny population derived from transgenic Chinese cabbage ‘Shanghaiqing’ (Brassica campestris ssp. chinensis var. communis cv. Shanghaiqing) line T0-9 inserted by sporamin. M: molecular marker, P+: positive control pCAMBIA1300PMSN-PMCN plasmid, WT: wild type plants, 1-30: T1 progeny plants of line T0-9.

Supplementary Fig. 4Sporamin expression in T1 progeny population of transgenic Chinese cabbage (Brassica campestris ssp. chinensis var. communis)was verified by semi-quantitative RT-PCR analysis. (A) Sporamin expression in T1-2 population of transgenic ‘Youdonger’. (B) Sporamin expression in T1-9 population of transgenic ‘Shanghaiqing’. WT: wild type plants, numbers indicate the different T1 progeny plants

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