JEANNETTE N. STANKOWSKI

Home:Work:

7221 Highway 70 South, Apt. 624465 21st Avenue South

Nashville, TN37221MRB III, Room 8150

(615) 636-5583Nashville, TN37232

(615) 936-5548

EDUCATION

VanderbiltUniversity, Nashville, Tennessee

Doctoral Degree Candidate, Neuroscience Graduate Program

Thesis Title: Role of HSP70 Interacting Protein CHIP in Determining Cell Fate Following Acute Ischemic

Injury.

Relevant Coursework:

  • Bioregulation
  • Cellular and Molecular Neuroscience
  • Systems Neuroscience
  • Neuroanatomy
  • Advanced Molecular Neurobiology
  • Neuroscience Foundations
  • Independent Research

DrewUniversity, Madison, New Jersey

Bachelor of Arts, Dual major in Biology and French, May 2004, GPA: 3.6/4.0

Relevant Coursework:

  • Cell and Molecular Neurobiology
  • Anatomy and Physiology of Neural Systems
  • Research in Biology
  • Medical Bacteriology
  • Virology
  • Vertebrate Morphogenesis
  • Behavior and Experience: An Intro to Psychology
  • Chemistry (Inorganic and Organic)
  • Physics

Activities:

  • Board of Visitors
  • Beta Beta Beta (Biology National Honor Society)
  • Pi Delta Phi (French Honor Society).

HONORS AND AWARDS

  • Society for Neuroscience 2009 National Chapter Graduate Student Travel Award, August 2009
  • VanderbiltKennedyCenter Travel Award for VKC Affiliates, March 2009
  • Neurotoxicology Specialty Section of Society of Toxicology Conference Award, July 2008
  • VanderbiltKennedyCenter Travel Award for VKC Affiliates, January 2008
  • Vanderbilt University Neuroscience Departmental retreatbest poster by a graduate student in cellular and molecular neuroscience Award, June 2007
  • Neuroscience Graduate Student Travel Grant, October 2006
  • Vanderbilt Predoctoral Training Fellowship, 2006-2008
  • Novartis Science Award for Excellence in Neuroscience, April 2004 – DrewUniversity
  • Dean’s List, (7 out of 8 semesters) – DrewUniversity
  • Everett Balch Endowed Scholarship, 2003-2004 – DrewUniversity
  • Drew Recognition Award, 2001-2004 – Drew University
  • Richard and Pat Fowler Scholarship, 2000-2004 – DrewUniversity

TECHNIQUES

  • Cell Culture (neural cell lines and primary neuronal cultures)
  • Immunoblotting
  • Immunofluorescence
  • Proteasome activity assays
  • Detection of glutathione levels using the HPLC methodology
  • DNA/RNA extraction from cells and tissues
  • Extraction and Generation of DNA from transformed E.coli
  • Transfections (Nucleofection, Lipid-based transfections)
  • Generation of Stable Cell lines
  • Small interfering RNA methodology
  • Protein carbonyl assays using the OxyBlot methodology
  • Biotin-avid capture and immunoblotting methodology
  • Generation and maintenance of hippocampal brain slices
  • Induction of acute injury (oxidative and denaturing) in neural cell lines and primary neurons
  • In vitro toxicology assays (LDH assay, MTT assay)

PUBLICATIONS

Stankowski, J.N., Zeiger, S.L. and McLaughlin, B. Mitochondrial status and oxidative damage. Cell Culture Techniques. Editor: Aschner, M. [In preparation]

Stankowski, J.N., Codreanu, S.G., Liebler, D.C. and McLaughlin, B. Effects of the E3 ubiquitin ligase CHIP on protein oxidation and antioxidant status. [In preparation]

Stankowski, J.N.,and McLaughlin, B. Role of Hsp70 interacting protein CHIP in determining cell fate following ischemic injury. [In preparation; to be submitted November 2009].

Stanwood, G.D., Leitch, D.B., Savchenko, V., Wu, J., Fitsanakis, V.A., Anderson, D.J., Stankowski, J.N., Aschner, M., and McLaughlin, B. (2009) Manganese Exposure is Cytotoxic and Alters Dopaminergic and GABAergic Neurons within the Basal Ganglia. Journal of Neurochemistry.

Stankowski, J.N., Leitch, D.B., Aschner, M., McLaughlin, B. and Stanwood, G.D. (2008) Selective Vulnerability of dopaminergic systems to Manganese: Relevance to occupational exposure. Neurotoxicology and Teratology , 30: 259.

ABSTRACTS

  • POSTER: Role of HSP70 Interacting Protein CHIP in Determining Cell Fate Following Ischemic Injury. Stankowski, J.N., Cohen, E., Codreanu, S.G., Liebler, D.C. and McLaughlin, B.M. Annual Meeting of the Society for Neuroscience (2009).
  • TALK: Selective Vulnerability of Dopaminergic Systems to Manganese: Relevance to Occupational Exposure. Stankowski, J.N., Leitch, D., Aschner, M., McLaughlin, B.M. and Stanwood, G.D. Thirty-second Annual Meeting of the Neurobehavioral Teratology Society (2008).
  • POSTER: Role of HSP70 Interacting Protein CHIP in Determining Cell Fate Following Ischemic Injury. Stankowski, J.N. and McLaughlin, B.M. Annual Meeting of the Society for Neuroscience (2006).
  • POSTER: Interaction between HSP70 and CHIP Leads to Protection Against Cell Death. Stankowski, J.N. and McLaughlin, B.M. Molecular Chaperones and Heat Shock Response in ColdSpringHarbor (2006).

CONFERENCES

  • Society for Neuroscience Conference, October 15-21, 2009
  • VanderbiltKennedyCenter Science Day, January 29, 2009
  • Thirty Second Annual Meeting of the Neurobehavioral Teratology Society, June 28-July 2, 2008
  • VanderbiltKennedyCenter Science Day, November 27, 2007
  • Society for Neuroscience Conference,October 13-18, 2006
  • ColdSpringHarbor Laboratory, Conference for Molecular Chaperones and the Heat Shock Response, May 3-7, 2006

PROFESSIONAL MEMBERSHIPS

  • Vanderbilt Kennedy Center Graduate Student Affiliate, November 2007-current
  • Member of Middle Tennessee Chapter Society for Neuroscience, June 2006-current
  • Member of Society for Neuroscience, May 2006-current

ADDITIONAL EXPERIENCE

United Nations Volunteers,St. Louis, MO02/05-07/05

Translator

Translated documents in the field of Economics from English into German; Translated documents in the field of Global Issues and Economics from English into French.

DrewUniversity, Madison, NJ, 01/04-03/04

Laboratory Technician

Prepared materials for all biology laboratories; Animal care.

DrewUniversity, Madison, NJ09/03-12/03

Teacher Assistant

Worked in the laboratory to prepare required materials for Prof. Knowles’ neurobiology class and

assisted during laboratory periods.

Human Genetic Research Institute, Oberhausen, Germany, Summer 2003

Internship, Genetic Analyst

Examined metaphase chromosomes to determine chromosomal abnormalities in embryos; Examined

FISH probes for the presence of Trisomy 13, 18, and 21.

DrewUniversity, Madison, NJ, 09/02-05/03

Peer Tutoring Program, Tutor (French, Physics), Typist

Worked on one-to-one basis with tutees and made material comprehensible to tutees.

LANGUAGES/INTERESTS

Native German speaker (German Abitur); fluent inEnglish, French and Polish. Enjoy traveling, languages, running, dancing and skiing.

REFERENCES

Dr. BethAnn McLaughlin, Assistant Professor of Neurology and Pharmacology.

EMAIL: ; PHONE: (615) 936-3847.

Dr. Pat Levitt, Provost Professor of Neuroscience, Psychiatry and Pharmacy.

EMAIL: ; PHONE: (323) 442-2144.

Dr. Mark Wallace, Associate Professor of Hearing and Speech; Director, Vanderbilt Brain Institute.

EMAIL: ; PHONE: (615) 936-6709.