Intron Analysis of Crmine. up Is CDS Sequence ,Down Is Genome Sequence

Supplements

Figure 1S

Thesequences information of CrMinE CDNA. The primers for RT-PCR are paired with N-end and C-end sequences.

Figure 2S

Intron analysis of CrMinE. up is CDS sequence ,down is genome sequence.

Figure 3S

Quantitative localization Analysis of CrMinE:EGFP. An average data of three

examined samples for each time point is presented. P<0.05.

Figure 4S

Immuno-blot analysis was done with polyclonal anti-GFPantibodies.CrMinE:EGFP were expressed inArabidopsis mesophyll protoplasts. 2.5μg or 5 μg ofproteins extracted from wide type protoplasts and CrMinE:EGFPprotoplastswere applied to each lane for SDS-PAGE andimmuno-blot analysis.

Gene information for construction of a phylogenetic tree of MinE：

>gi|116794432|gb|ABK27141.1| unknown [Picea sitchensis]

MMAFQPTTTSRLDIHPPLLLRNPAFFSKVDRFKCSTSFVRDCKSMLPLQMPMQLHLALGRYHVGKSHRCYGVGDDGLSNVSITQDIDDFICKAVNMNFFERLNWAWNILFPRKLKRTSNAEIAKQRLKMILFSDRCAVTDDAKRKIVNNIVGALSDFVEIDSEDKVQLNVSADPDLGTVYSVTVPVRRVKPEYQEYSEGFGDIRNLQYKDISGQVSAFDIKLDYPSPNDGKA

>gi|743865881|ref|XP_010904930.1| PREDICTED: cell division topological specificity factor homolog, chloroplastic-like isoform X1 [Elaeis guineensis]

MAISGDLKAFATFASYHPKHPLRSVLSSSKVQLGRFLNVGSNDLKITPKWPYMETPTSNTAHNGHQCFGFGANKLSSTTITQDAEGILLDAINMNFFDRLSLAWRILFPTRTARRNSNARIAKQRLKMILFSDRCAVSDEARKKIVRNIIEALSEFVEIDSQDKVQLNVSTDPDLGTVYSVTVPVRRVKPEYQDSEEDYRGKITGVEYKDTGETSGTVDVTFDFFIPNEK

>gi|769811173|ref|XP_006844856.2| PREDICTED: cell division topological specificity factor homolog, chloroplastic [Amborella trichopoda]

MAIVPSTYFENTITMISSSASFSALSNKRPSLSPSTLFSHPSIFIPSYSTKVGFPGNPIRGMHTFGHIPKWPCVAVESHHPQQISHQSFSLGDNLSSSTTSQEAEGLLLDAVKMSFFERLSLAWKILFPTIVKKSTNAKIAKQRLKMILFSDRCAVSDEAKRKIVSNIVGALSNFVEIESQDKVQLSVSTDPDLGTVYSVTVPVRRVKPEYQDSGDSYRGISNIEFKDTGEKSGSVDVRFDFFLPANEE

>gi|685273363|ref|XP_009127725.1| PREDICTED: cell division topological specificity factor homolog, chloroplastic-like [Brassica rapagl]

MAISGNLRISASLISPYHHHPKCLSLPSSKVDFISNVANSLETQKVSISSKNKRGEAKVLARNATGDYELSPSPVQQEVESFLLNAINMSFFDRLNIAWKIIFPSHASRRSSNARIAKQRLKMILFSDRCAVSDEAKRKIVNNIVHALSDFVEIESEEKVQLNVSTDGDLGTIYSVTVPVRRVKPEYQDVDEAGSITNVEYKDTLDGSVDVRFDFYVPE

>gi|548847347|gb|ERN06531.1| hypothetical protein AMTR_s00058p00104170 [Amborella trichopoda]

MHTFGHIPKWPCVAVESHHPQQISHQSFSLGDNLSSSTTSQEAEGLLLDAVKMSFFERLSLAWKILFPTIVKKSTNAKIAKQRLKMILFSDRCAVSDEAKRKIVSNIVGALSNFVEIESQDKVQLSVSTDPDLGTVYSVTVPVRRVKPEYQDSGDSYRGISNIEFKDTGEKSGSVDVRFDFFLPANEE

>gi|672132963|ref|XP_008790079.1| PREDICTED: cell division topological specificity factor homolog, chloroplastic-like isoform X2 [Phoenix dactylifera]

MAISGDLKAFGTFSTYHPKHPLRPALSSSKLGRFLNAGSNDLRITPKWPYIMTPTSNTVHNSHRCFVFGGNKLSSTTITQDAEGILLDAINMNFFDRLSLAWRILFPTRTARINSNARIAKQRLKMILFSDRCAVSDEARKKIVRNIIEALSEFVEIDSQDKVQLNVSTDADLGTVYSVTVPVRRVKPEYQDSEEDYQGKITGIEHKDTGETSGTVDVTFDFFIPSGK

>gi|672132961|ref|XP_008790078.1| PREDICTED: cell division topological specificity factor homolog, chloroplastic-like isoform X1 [Phoenix dactylifera]

MAISGDLKAFGTFSTYHPKHPLRPALSSSKVQLGRFLNAGSNDLRITPKWPYIMTPTSNTVHNSHRCFVFGGNKLSSTTITQDAEGILLDAINMNFFDRLSLAWRILFPTRTARINSNARIAKQRLKMILFSDRCAVSDEARKKIVRNIIEALSEFVEIDSQDKVQLNVSTDADLGTVYSVTVPVRRVKPEYQDSEEDYQGKITGIEHKDTGETSGTVDVTFDFFIPSGK

>gi|17511220|dbj|BAB79236.1| AtMinE [Arabidopsis thaliana]

MAMSSGTLRISATLVSPYHHHHRNRLSLPSSSSKVDFTGFISNGVNSLETQKCTPGLAISRENTRGQVKVLARNTGDYELSPSPAEQEIESFLYNAINMGFFDRLNLAWKIIFPSHASRRSSNARIAKQRLKMILFSDRCDVSDEAKRKIVNNIIHALSDFVEIESEEKVQLNVSTDGDLGTIYSVTVPVRRVKPEYQDVDEAGTITNVEYKDTRDGSVDVRFDFYVPE

>gi|674937286|emb|CDX95938.1| BnaA07g27930D [Brassica napus]

MAITGNLRISASLLTPYHQHPKCLSLPSSSKVDFSGFVSSGVNILEPYKCPPGVSRAQTKVLARNTGGDYELSPSPAEQEVESFLLNAINMSFFDRLNLAWKIIFPSHASKRSSNARIAKQRLKMILFSDRCAVSDEAKRKIVNNIVHALSDFVEIESEEKVQLNVATDGDLGTIYSVTVPVRRVKAEYQDVDEVGSITNVDYKDTLDGSVDVRFDFYVPE

>gi|351722701|ref|NP_001236997.1| plastid division regulator MinE [Glycine max]

MAISGDLRVSATLPLYRSHSPPLRTSSQSSPKVDFHRFLNRACSISEFTPKCPYLTIVRCNMRGYCKPVAAVLGGPKFTSNSVSQETENFLLDAVKMNFFERLNLAWKIIFPSATSRKNSNARIAKQRLKMILFSDRCEVSDEAKRKIVSNVVRALSDFVEIESQDKVQLSVSADTDLGTIYSVTVPVRRVKPEYQEMDEFGTITNVEYKDTGETSGSVDVTFDFYVPDERS

>gi|566191250|ref|XP_002315021.2| chloroplast division family protein [Populus trichocarpa]

MAISGDLRVSATLASYSKHPPRCSLPPSNSKVEFLGFLNGGCGTSQNMLKWPGGVFDSRKMHGHFKRSAGIAEEYQLSSTAINQEAERLLLSAINMSLFERLNLAWRIIFPSPTQRKSSNARIAKQRLKMILFSDRCAVSDEAKRKIVNNIVHALSEFVEIESQDKVQLSVTTDTDLGTVYSVTVPVRRVKPGYQEEESGSITNIEYKDTGENSGSVDVRFDFYLPDERTRF

>gi|1002310555|ref|XP_015618636.1| PREDICTED: cell division topological specificity factor homolog, chloroplastic [Oryza sativa Japonica Group]

MAMATSISAGFGGEPGAVLAPSASVLPAPRRRNPATSSKAQFSSFPRGRSCNIMLTPKHLGIEHQSCSKSSIQTFALSRNDFSPIAQEVEGFLHNIVNMGFLDRLKLAWKIIFPAPSIKENSNANIAKQRLKMILFSDRCEVSDEAKKKIVENIVEALSEFVEIESRDNVQVDISTDAGLGTVYSVTVPVRRVKPEYQESEEQYRGKIVGVDFKDTGETSGSVDVTFDFFVPNKNY

>gi|71089835|gb|AAZ23774.1| plastid division regulator MinE [Hordeum vulgare]

MATATAISCRVSGEASAILAPSASSLPAPLRRAANSKGCFRAFPRGTSSRLMLTLGRLRVERQSCLVRSSACSALSRRDFSPVTQDMDGFLHSIVNMGFLDRLKLAWEIIFPAPTIHENTNASIAKQRLKMILFSDRCEVSDEAKKKIVESVVEALSEFVEIESRDNVQVDISTDAGLGTVYSVTVPVRRVKPEYQESEEQYGGKIVGVDFKDTGESSGNVDVTFDFYVPNENH

>gi|40217512|dbj|BAD05165.1| MinE [Zea mays]

MAMSIFVGGDSGALIAPSASSLRPPFRLVAGPKGHFCTFPHGASSNHSRLCVQHQRCSRSSTQNYALSRKDFSPITQDVEGFLHNVVNMGFLDRLKLAWKIMFSAPTTKDTSNANIAKQRLKMILFSDRCEVSDEAKKKIVENIIEALSEFVEIESRDNVQVDISTDAGLGTVYSVSVPVRRVKPEYQESEEQYRGKIVGVDFKDTGESSGSVDVTFDFFVPNEN

>gi|117557337|gb|AAQ19801.2| MinE [Chlamydomonas reinhardtii]

MEALRCQSRVLGAGHAAATASAVRRPALSLVSHSVRTPVQRQAAYYETDLPLANIAKATRTVPKAPKPTGNAVNDLVTKLQLCFKIFFPDEEPKVNVTPKEEVERRLKMVLVADRCGMSPNSLGELKRTIARALQDFVELESEDAIEVNISDDPGLGTVYCVAIPVRRVKAESRFAEGRLEAGPDGVVLEWDPTDLHSDPSSRFPLGC

>gi|4775296|emb|CAB42593.1| putative MINE protein [Auxenochlorella protothecoides]

MATLLQQGTFAPHRSWSGRKGTRRVSKPTLNRLHVRSSSKAGAGPVSDAHLAHLRNAGHPVPEAPGLQGFVAKLKAAWQIFFPEKPPVLTPKDEGKNRLRMILVADRCGITPDSLTGMRESIVQAVSAYVDIETEEEIEVNLSTDPELGTIYSVAVPVRRVKSRRIGGVDTSEDGKIIVKWDPKDPNSDPSDQFPFGV

>gi|499306832|ref|WP_010997607.1| cell division topological specificity factor [Nostoc sp. PCC 7120]

MILELLDKLFLRTPDTSRSHVKRRLQLVIAHDRAGLDPETLEKMRKEILDIVCRYVEVEPDGLEFALESNQRTTALIANLPIRRVKPELPIFDETSQ

>gi|501378444|ref|WP_012410010.1| cell division topological specificity factor [Nostoc punctiforme]

MIELLEKLFSRGPDSSRTQVKRRLQLVIAHDRADLDPQTLEKMRKEILDVVCRYVEIETEGLEFSLESNQRTTALIANLPIRRVKGAIPEWERGDK

>gi|499370275|ref|WP_011057853.1| cell division topological specificity factor [Thermosynechococcus elongatus]

MISELLERLFTRTPPSRTTAKERLKLVLAHDRTALTPEILDNLRRDILEVVSRYVELETEGLAVSLESDQRTTALIANLPIKRLKLSPEDAPLPENFPPENEAKER

>gi|11467634|ref|NP_050686.1| septum site-determining protein (chloroplast) [Guillardia theta]

MITEFFERLFLSNKGSREDVKRRLKLVLAHDRSTLNASTLEKMREEILLVVSKYVELDTDSLEFSIRTDSKMTALIANLPIRRILKDI

>gi|499176302|ref|WP_010873889.1| cell division topological specificity factor [Synechocystis sp. PCC 6803]

MILELIERLFSRSGKNSGEDARRRLKLVIANDRSGLSPEMMEEMRREIVEVVSRYVEIDPGEMEFSLESDQRMTALIANLPVRRVRRTKAKSEAQES

>gi|499441366|ref|WP_011128830.1| MULTISPECIES: cell division topological specificity factor [Synechococcus]

MTLQDLIDKLLGRQPASADTARQRLQLVLAHDRSDLNPELLDQMRREILEVVSRYVEIDLSEGDVSLETEDRVTALVANLPIRRTL

>gi|499443557|ref|WP_011131021.1| cell division topological specificity factor [Prochlorococcus marinus]（1）

MATTLRDILDKLLGRQPASAKTARERLQLVLAHDRSDLSPELLDQMRREIFEVVAKYVEIDLEEGEVSLETEDRMTALVANLPIKRSQAKAVSSQENGASSQEAVSSQESVSTPGAME

>gi|499437053|ref|WP_011124517.1| MULTISPECIES: cell division topological specificity factor [Prochlorococcus]

MTLRDILNKLMGRQPASAAKARERLQLVLAHDRTDLSPDLLEQMREEILSVVAKYVEIDFEEGAVSLETEDRMTALVANLPIKRTLSGEIKIKEQSSADNSAKAVS

>gi|499444493|ref|WP_011131957.1| cell division topological specificity factor [Prochlorococcus marinus]（2）

MMTLRDLINKLLGRETASANTARERLQLVLAHDRVDMSSLTTDLLDKMRKEILDVVAKYVEIDFEEVAVSLETEDRMTALVANLPIKRTLTGEIEFKKNEDTQKKK

>gi|499454528|ref|WP_011141992.1| cell division topological specificity factor [Gloeobacter violaceus]

MVFDLLERLFNRNKTGSATVAKDRLKMVLAVDRTEIAPQTIEQIRKEILDVIVRYFEIDENEKFDVTLERERGSTAIIANVPIRRIRPEHI

>gi|693256681|ref|WP_032359843.1| cell division topological specificity factor [Escherichia coli]

MALLDFFLSRKKNTANIAKERLQIIVAERRRSNAEPHYLPQLRKDILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK

>gi|488175532|ref|WP_002246740.1| cell division topological specificity factor [Neisseria meningitidis]（2）MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLRKELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV

>gi|488144257|ref|WP_002215465.1| cell division topological specificity factor [Neisseria meningitidis]（1）

MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLRKELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV

>gi|445973557|ref|WP_000051412.1| cell division topological specificity factor [Helicobacter pylori]

MSLFDFFKNKGSAATATDRLKLILAKERTLNLPYMEEMRKEIIAVIQKYTKSSDIHFKTLDGNQSVETIEVEIILPK

>gi|499189857|ref|WP_010887397.1| cell division topological specificity factor [Deinococcus radiodurans]

MFGWFKGRRSKETLKDRLELVLAYDRAKIAPGKVEALRNDLLEVVQKYFPSGNSKVEVEQDGDKVVLMASISIEEASLSTEEESAAEDKSDKPSA

>gi|7524871|ref|NP_045873.1| cell division topological specificity factor (chloroplast) [Chlorella vulgaris]

MVELNFTQKQKQFLKDRKSLKSLVNQHLASDLGETSLSQYLDEFQILYNRFFCFYFFSYGENVFCSEEIFSSQLKNGFFPTKTKDEILSSPALNVIFSEKDFLLLKNIRIFLGMDSNNFFLTEELFN

>gi|971509751|dbj|GAQ88458.1| hypothetical protein KFL_004300090 [Klebsormidium flaccidum]

MSASTCARGGLASCSNRVHGTQARTDKPDGLPSLRHPLRIHFATHSGLKCLHFPRNPEPPHSVSGKHSPAQASTGRKAAKPKPEVAEAALEEDRPPRSFWEKLVAAFEIFFPPAQVQEAPRQRAKQRLRMILVADRCSVSPTSVSQIKKAIVESLSNFVEIEQEDKVDLNITTDPNLGTIYSVSVPVRRVKPEFDIQTEYENKEFYFDSDGEQIRSFDLSFEDMQPDVVGSKDGASVPAPKLPDAVQRRRGPVLPDAPFVDGRGPPDGPT

>gi|255078228|ref|XP_002502694.1| plastid division regulator [Micromonas commoda]

MQANTRARVGASRRASVAVRADYDAGERRGRDTTFGMRQQMLEKKDKGADTSSFWGKVKAAFAIFFPPSEEETARLEAKKRLRMILVADRCAMSGAAMSLMKTRIVEVVSDFVDVDEELGVEVSMSQDPEVTGTMYAVSIPVRGVKPQFDVENETYGWDEEPMYDDDVWEKSESDK

>gi|302761908|ref|XP_002964376.1| hypothetical protein SELMODRAFT_405578 [Selaginella moellendorffii]（1）

MLRSVHCTGRIGGCLAEDRNFSFPGSRKLPPRSARLSSRFRSSPAQPIRALEQDLQHQRLDIEEEKRSGGFLERLARAWEILFPPEDTAAARPFASNAEIAKQRLKMILISDRCSITDDAKKKIVTNIVGALADFVEIESEEKVQLNVLADPELGTVYSVTVPVRRVKPEYQEFSKEIEQGLEYQPVGDSGARTVAIKLEES

>gi|302786540|ref|XP_002975041.1| hypothetical protein SELMODRAFT_415331 [Selaginella moellendorffii]（2）

MEHMSLHGYIERAHFQIHTGFRRLTPRAKRNSTRCSVSHQEQSCSNRVPTFWERLLLAWQVLFPPSNSSAETSANIAKQRLKMVLIADRCAVSDAAKRMIVSNVVGAMSHFVEVESEEKVQLNVTTDSELGTIYSITIPVRRVKPEYQLSSKDLEGVELAEGLTCNIVLDPSREP

>gi|548847347|gb|ERN06531.1| hypothetical protein AMTR_s00058p00104170 [Amborella trichopoda]

MHTFGHIPKWPCVAVESHHPQQISHQSFSLGDNLSSSTTSQEAEGLLLDAVKMSFFERLSLAWKILFPTIVKKSTNAKIAKQRLKMILFSDRCAVSDEAKRKIVSNIVGALSNFVEIESQDKVQLSVSTDPDLGTVYSVTVPVRRVKPEYQDSGDSYRGISNIEFKDTGEKSGSVDVRFDFFLPANEE

>gi|145355309|ref|XP_001421906.1| predicted protein [Ostreococcus lucimarinus CCE9901]

MKMGRATRATTRTRAIANEAQTSEELNFWGKMRQAYRIFFPPSAEENARNEAKKRLRMILVADRCTMSESSMDEMKAKIMTVVGEFVDVDDSQEVDVSMNTDEELGTMYAVSIPVKRVKAEYDAENDAYMLGAQFVYDDENVWDDE

>gi|545372651|ref|XP_005650774.1| hypothetical protein COCSUDRAFT_12561, partial [Coccomyxa subellipsoidea C-169]

AGNGKAPAGGLADFARKLRAAWQIFFPPPVKDISPKEEGKNRLRMILVADRCGMNPASLHDMKHSIVRAVQDYVDIEAEELIEVNISTDVDLGTIYSVALPIKRVKPVARVPMEDDVDVDGITLRWDSDDPESDPSSEFPFGC

>gi|926777542|ref|XP_013897245.1| chloroplast division site-determinant MinE [Monoraphidium neglectum]

MRAQSSANCTTVASSCTPAAPRQQPPRARASRLTTAAASIGARGGAATAAAPPGGRQRERTLTLGTGELTATSSAVGTSINYRIRRDGTGGGGVKDFLDKLWLVWQIFFPERQPDTTPKDGAKQRLRMILVADRCGLSPSGLSEMKKNILRALEEFVDIETEEEIDVSISIQPDLGTIYCVAVPVKRVKPEARMGLDGGADGMDVFEWDPEDKESDPSSRFPMGC

gi|168018071|ref|XP_001761570.1| predicted protein [Physcomitrella patens]（1）

MCAVASRSHRVHCSTVVPLPLGSWKVNKRPCGFSSFPDKHLVGGRVRIFQVPGADSDICDNSQNRKLSPCRAQFDERQPSRMNNEEAFSSEEKEESFFERLVRAWAIIFPVKSNPSSNASIAKQRLKMILISDRCSVSDDAKRRIVTNIVGALSDFVEIESEDKIQLNVSSDPDLGTVYSVTVPVRRVRPQYQEFSHELVNTELRSFDYEEEEGNFRMVDIRFEYPDVFHKEVVPDAFQDDDDDVQGSEDGSTSNLPDS

>gi|168001327|ref|XP_001753366.1| predicted protein [Physcomitrella patens]（2）

MCVVAVRSLRVHCPAVVSLPLESWKVNKNLCHFLPSPDKHRVGSRLKISQVPNANLDRWDSPHNRRLPPCRAHFDEEQLSRSNYPNNAEGFPGEEKESFLERLARAWAILFPAKSNPSSNASIAKQRLKMILISDRCSVNDDAKRRIVTNIVGALSDFVEIESEDKVQLNVSSDPDLGTVYSVTVPVRRVRPQYQEFSHELVNTELRTFDYEEVEGNFRMVDIRFEHPDVFHQDVVPDGFQDNSDVQESEDGSAPNV

>gi|168059874|ref|XP_001781925.1| predicted protein [Physcomitrella patens]（3）

MLQSAQGPSARVHFLRHAHYRNMSRCIAMKSRVENSRADFSTGNEEDEITGGKSQTFMERITRAWSVLFPAKAKPSSNKDVAKQRLKMILISDRCTVNEEAKKKIVTNIVGALSDFVEIQSEEKVQFNVTSDADLNTVCSVTVPVRRVRPQYQEFSRDLVHSELEAFDCEEKEGSFRMVDIRFERPDVEGIDV