SCHA 319
Separations Laboratory
Spring 2007 (20063)
InstructorsMeeting times:
Dr. Paul RosenbergSections: 40 Monday (2-6), 42 Tuesday (8-12)
Mr. Ian CawthraySections: 41Monday (2-6), 44 Thursday (8-12)
Ms. Luciana OliveriaSections: 43Tuesday (8-12), 44 Thursday (8-12)
Lab will be held in 08-A335. We will also use the instrument room (A143) but A335 will we the home lab with your drawer assignment.
ATTENDANCE:.
Attendance to the laboratory is mandatory. If you are absent from your scheduled lab period you must notifiy your instructor and you will be required to make up your work. If you habitually arrive late for the laboratory, or leave your lab partners to complete the laboratory, you will automatically fail the course. YOU MUST WEAR SAFETY GOGGLES AT ALL TIMES!
GRADING.:
3 full lab reports45%
3 short reports25%
lab notebook10%
evaluation of experimental ability20%
Lab books will be collected at the end of the quarter. The lab reports are due
7 days after completion of the experiment. ALL LAB REPORTS MUST BE HANDED IN AT THE BEGINNING OF LAB MW DETNTION. Late lab reports will be severely penalized.
SCHA 319 Lab Rotation Schedule
Laboratory experiments
A) Extraction of Mandelic AcidROOM - 08 – A335
B) Column Chromatography of Food Dyes(short report)ROOM - 08 – A335
C) Size Exclusion Chormatography/LC-MS (short report)ROOM - 08 – A143 (MW Detn)
D) HPLC of ExcedrinROOM - 08 – A143
E) GC with a Mass Selective DetectorROOM - 08 – A143
F) Solid Phase Extraction of Caffeine (short report)ROOM - 08 – A335/A143
YOU MUST BE PREPARED BEFORE YOU ENTER THE LABORATORY. NO TIME EXTENSIONS ARE POSSIBLE ON ANY OF THE INSTRUMENTS. YOU ARE REQUIRED TO HAVE A PROTOCOL WRITTEN UP IN YOUR NOTEBOOK BEFORE YOU ENTER THE LABORATORY.
Schedule for all MW Detntions. Groups and group numbers will be assigned the first week of lab.
Group 1 / Group 2 / Group 3 / Group 4 / Group 5 / Group 6 / Group 7 / Group 8Week 1 3/12 / Check In / Check In / Check In / Check In / Check In / Check In / Check In / Check In
Week 2 3/19 / Solvent Extraction / Solvent Extraction / Solvent Extraction / Solvent Extraction / Solvent Extraction / Solvent Extraction / MS / HPLC
Week 3 3/26 / MS / HPLC / TLC / TLC / TLC / TLC / Extraction / Extraction
Week 4 4/2 / HPLC / MS / TLC / TLC / TLC / TLC / TLC / TLC
Week 5 4/9 / MW DETN / MW DETN / MS / HPLC / SPE / SPE / TLC / TLC
Week 6 4/16 / TLC / TLC / HPLC / MS / MW DETN / MW DETN / SPE / SPE
Week 7 4/23 / TLC / TLC / MW DETN / MW DETN / MS / HPLC / Off / Off
Week 8 4/30 / SPE / Off / SPE / Off / HPLC / MS / MW DETN / MW DETN
Week 9 5/7 / SPE / SPE / HPLC / MS
Week 10 5/14 / Check-Out / Check-Out / Check-Out / Check-Out / Check-Out / Check-Out / Check out / Check-Out
Lab Reports are due one week after the completion of the lab.
SCHA 319
Spring 20063
Lab Report Format
All reports should be submitted in Word format and will be submitted on paper. The reports will also be submitted as Word documents to You will need to register at this web page. Our Class ID is 1842643and the password is luiscolon
LATE LAB REPORTS WILL BE SEVERELY PENALIZED!!!!!
Full lab reports are to consist of the following:
1)ABSTRACT - a one paragraph summary of the entire experiment. This includes basic theory, fundamental procedure, final results and brief conclusions. See examples in the journal ANALYTICAL CHEMISTRY. In brief – tell what you set out to do, how you did it and what the results were. (15 points)
2)INTRODUCTION - A presentation of background information. This section will include a short theory section, (2 - 3 paragraphs) that demonstrates your understanding of the experiment. (20 points)
NOTE: Plagiarism will not be tolerated. If a student is found to have plagiarized their report from either the chemical literature or from a current or past student will receive a grade of F for the course.
3)EXPERIMENTAL - only modifications to protocol need to be mentioned. Otherwise it should be a one line statement, "The experimental procedure was performed as per handout." (5 points)
4)DATA - A clear presentation of all information gathered in the laboratory. Tables and graphs are necessities and should be formatted and labeled in standard format. (20 points)
5)CALCULATIONS - A sample of any numerical calculations, presented in a logical fashion.
(10 points)
6)RESULTS/DISCUSSION - The most important portion of the report. Clearly explain your data and demonstrate how it does or does not fit the theory. Accuracy and precision are important here as is a presentation of reasonable sources of error, a correctable error is not reasonable. Explain your experiment completely and succinctly! (20 points)
7)Answers to questions in lab handout. Although you might have covered them already please Include them here. (10 points)
These reports should be very concise and to the point.