Identification of Mir-199A-5P in Serum As Noninvasive Biomarkers for Detecting and Monitoring

Identification of miR-199a-5p in serum as noninvasive biomarkers for detecting and monitoring osteosarcoma

Supplementary Table 1. Differentiallyexpressed miRNAs in pre-operative osteosarcoma serum samples compared to healthy control serum samples determined by TLDA.

miRNA / Ct of healthy control / Ct of pre-operative osteosarcoma / ΔCt (pre-operative - control)
hsa-miR-198 / 40 / 15.39674 / -24.6033
hsa-miR-208b / 40 / 20.58845 / -19.4115
hsa-miR-675 / 40 / 23.43217 / -16.5678
hsa-miR-1233 / 40 / 24.64177 / -15.3582
hsa-miR-412 / 40 / 25.40381 / -14.5962
hsa-miR-130a / 40 / 25.64371 / -14.3563
hsa-miR-31# / 40 / 25.64864 / -14.3514
hsa-miR-365 / 40 / 25.68048 / -14.3195
hsa-miR-663B / 40 / 25.76969 / -14.2303
hsa-miR-874 / 40 / 26.58571 / -13.4143
hsa-miR-450b-5p / 40 / 26.67483 / -13.3252
mmu-miR-379 / 40 / 27.51486 / -12.4851
hsa-miR-549 / 40 / 29.78017 / -10.2198
hsa-miR-548G / 40 / 30.49524 / -9.50476
hsa-miR-582-3p / 40 / 31.62882 / -8.37118
hsa-miR-222# / 40 / 31.77401 / -8.22599
hsa-miR-199a-5p / 34.35248 / 26.51492 / -7.83756
hsa-miR-888 / 40 / 33.72916 / -6.27084
hsa-miR-424# / 40 / 33.73022 / -6.26978
hsa-miR-548J / 40 / 33.74674 / -6.25326
hsa-miR-219 / 21.72982 / 26.74239 / 5.012574
hsa-miR-302c / 23.94207 / 29.51788 / 5.575815
hsa-miR-1274A / 18.98554 / 24.60655 / 5.621015
hsa-miR-183 / 25.01077 / 30.64012 / 5.629349
hsa-miR-133b / 23.03574 / 28.6739 / 5.638162
hsa-miR-625# / 20.98978 / 26.6612 / 5.67142
hsa-miR-886-3p / 27.95824 / 33.64481 / 5.686569
hsa-miR-301 / 28.01681 / 33.70892 / 5.692108
hsa-miR-218-1# / 21.886 / 27.58015 / 5.694154
hsa-miR-320B / 22.95512 / 28.64946 / 5.694335
hsa-miR-1183 / 24.94727 / 30.64184 / 5.694563
hsa-miR-410 / 34.03893 / 39.73606 / 5.697127
mmu-miR-374-5p / 22.9964 / 28.69538 / 5.698982
hsa-miR-1179 / 30.99432 / 36.69757 / 5.703249
hsa-miR-181a-2# / 25.98357 / 31.68739 / 5.703813
hsa-miR-99b / 22.01707 / 27.72108 / 5.704014
hsa-miR-370 / 24.99092 / 30.69637 / 5.705444
hsa-miR-361 / 25.99186 / 31.70077 / 5.708912
hsa-miR-542-3p / 34.02685 / 39.73606 / 5.709209
hsa-miR-181a / 21.99351 / 27.70372 / 5.710206
hsa-miR-101 / 24.98224 / 30.70112 / 5.718884
hsa-miR-30c / 17.97858 / 23.70172 / 5.723135
hsa-miR-193b / 23.96318 / 29.68653 / 5.72335
hsa-miR-618 / 22.96696 / 28.69533 / 5.728366
hsa-miR-29c / 25.98351 / 31.71431 / 5.730802
hsa-miR-106b / 21.96285 / 27.70922 / 5.746372
hsa-miR-1274B / 15.92335 / 21.67105 / 5.747697
hsa-miR-423-5p / 23.96158 / 29.70958 / 5.747997
hsa-let-7d / 20.9832 / 26.73242 / 5.749224
hsa-miR-340 / 23.97322 / 29.724 / 5.750778
hsa-miR-20a / 18.97075 / 24.72248 / 5.751731
hsa-miR-335 / 24.96772 / 30.72275 / 5.755029
hsa-miR-362 / 26.0057 / 31.76184 / 5.756149
hsa-miR-92a / 17.98431 / 23.74149 / 5.757179
hsa-miR-127 / 22.95538 / 28.71518 / 5.759794
hsa-miR-548a / 28.92534 / 34.68528 / 5.759941
hsa-miR-744# / 31.05878 / 36.82548 / 5.766704
hsa-miR-744 / 21.87909 / 27.64811 / 5.769014
hsa-miR-484 / 15.9082 / 21.67731 / 5.769112
hsa-miR-183# / 29.97447 / 35.74739 / 5.772917
hsa-miR-152 / 22.93759 / 28.71289 / 5.775298
hsa-let-7g / 20.96651 / 26.74295 / 5.776445
hsa-miR-504 / 13.6866 / 19.47679 / 5.79019
hsa-miR-576-3p / 25.91845 / 31.71087 / 5.792423
hsa-miR-362-3p / 33.92996 / 39.73606 / 5.806095
hsa-miR-374 / 20.92729 / 26.74693 / 5.819637
hsa-miR-204 / 24.88745 / 30.72133 / 5.833876
hsa-miR-200c / 23.06939 / 29.65879 / 6.589404
hsa-miR-99b# / 27.06883 / 33.68075 / 6.611913
hsa-miR-296 / 26.06418 / 32.71186 / 6.647676
hsa-miR-411 / 33.05787 / 39.73606 / 6.678192
hsa-miR-224 / 28.04752 / 34.73989 / 6.692373
hsa-miR-500 / 28.92502 / 35.63506 / 6.710037
hsa-miR-1267 / 20.97648 / 27.69034 / 6.71386
hsa-miR-720 / 15.95548 / 22.67072 / 6.715238
hsa-miR-758 / 25.99135 / 32.71179 / 6.720442
hsa-miR-367 / 32.9871 / 39.73606 / 6.748957
hsa-miR-30b / 17.97558 / 24.72723 / 6.751643
hsa-miR-1301 / 32.98415 / 39.73606 / 6.751903
hsa-miR-375 / 21.92673 / 28.68083 / 6.754109
hsa-miR-328 / 22.93042 / 29.68829 / 6.757875
hsa-miR-125b / 22.95031 / 29.7267 / 6.776397
hsa-miR-885-5p / 20.86743 / 27.6459 / 6.778474
hsa-miR-508 / 24.97501 / 31.75892 / 6.783914
hsa-miR-302a / 32.93213 / 39.73606 / 6.803932
hsa-miR-1260 / 19.02058 / 26.70261 / 7.682023
hsa-miR-132 / 22.9909 / 30.68921 / 7.698309
hsa-miR-20a# / 32.0185 / 39.73606 / 7.717557
hsa-miR-433 / 27.99561 / 35.73347 / 7.737863
hsa-miR-452 / 31.98136 / 39.73606 / 7.754701
hsa-miR-543 / 26.91858 / 34.6758 / 7.757229
hsa-miR-493 / 31.95473 / 39.73606 / 7.78133
hsa-miR-185 / 21.91743 / 29.71585 / 7.798425
hsa-miR-429 / 30.98038 / 39.73606 / 8.755677
hsa-miR-518a-3p / 30.95695 / 39.73606 / 8.779106
hsa-miR-142-3p / 26.02901 / 35.65677 / 9.627765
hsa-miR-369-3p / 30.01134 / 39.73606 / 9.724722
hsa-miR-203 / 29.9813 / 39.73606 / 9.75476
hsa-miR-517c / 29.97427 / 39.73606 / 9.761791
hsa-miR-512-3p / 29.01049 / 39.73606 / 10.72557
hsa-miR-125a-3p / 28.98728 / 39.73606 / 10.74878
hsa-miR-518e / 28.98329 / 39.73606 / 10.75277
hsa-miR-591 / 28.94996 / 39.73606 / 10.7861
hsa-miR-335# / 21.94863 / 32.7995 / 10.85087
hsa-miR-215 / 28.87094 / 39.73606 / 10.86511
hsa-miR-32 / 28.02569 / 39.73606 / 11.71037
hsa-miR-486-3p / 27.01434 / 39.73606 / 12.72172
hsa-miR-326 / 26.84722 / 39.73606 / 12.88884
hsa-miR-1 / 26.06369 / 39.73606 / 13.67237
hsa-miR-381 / 21.24196 / 35.74936 / 14.5074
hsa-miR-34a / 24.92975 / 39.73606 / 14.80631
hsa-miR-144 / 24.92715 / 39.73606 / 14.80891
hsa-miR-22# / 24.87597 / 39.73606 / 14.86009
hsa-miR-302d# / 24.84456 / 39.73606 / 14.8915
hsa-miR-505 / 24.07648 / 39.73606 / 15.65958
hsa-miR-107 / 23.94321 / 39.73606 / 15.79285
hsa-let-7e / 19.15034 / 39.73606 / 20.58572

Supplementary Table 2. Differentiallyexpressed miRNAs in pre-operative osteosarcoma serum samples compared to post-operative osteosarcoma serum samples determined by TLDA.

miRNA / Ct of pre-operative osteosarcoma / Ct of post-operative osteosarcoma / ΔCt (pre-operative - post-operative)
hsa-miR-337-5p / 13.68981 / 38.75712 / -25.0673
hsa-miR-198 / 15.39674 / 38.75712 / -23.3604
hsa-miR-216a / 17.63624 / 38.75712 / -21.1209
hsa-miR-193a-3p / 18.58956 / 38.75712 / -20.1676
hsa-miR-504 / 19.47679 / 38.75712 / -19.2803
hsa-miR-382 / 20.43196 / 38.75712 / -18.3252
hsa-miR-208b / 20.58845 / 38.75712 / -18.1687
hsa-miR-675 / 23.43217 / 38.75712 / -15.325
hsa-miR-216b / 24.4995 / 38.75712 / -14.2576
hsa-miR-412 / 25.40381 / 38.75712 / -13.3533
hsa-miR-219-1-3p / 25.5002 / 38.75712 / -13.2569
hsa-miR-130a / 25.64371 / 38.75712 / -13.1134
hsa-miR-31# / 25.64864 / 38.75712 / -13.1085
hsa-miR-365 / 25.68048 / 38.75712 / -13.0766
hsa-miR-103 / 25.69028 / 38.75712 / -13.0668
hsa-miR-376b / 26.43716 / 38.75712 / -12.32
hsa-miR-199a-5p / 26.51492 / 38.75712 / -12.2422
hsa-miR-874 / 26.58571 / 38.75712 / -12.1714
hsa-miR-26a-1# / 26.66803 / 38.75712 / -12.0891
hsa-miR-450b-5p / 26.67483 / 38.75712 / -12.0823
hsa-miR-219 / 26.74239 / 38.75712 / -12.0147
hsa-let-7g / 26.74295 / 38.75712 / -12.0142
hsa-miR-130a# / 27.4823 / 38.75712 / -11.2748
hsa-miR-708 / 27.5039 / 38.75712 / -11.2532
mmu-miR-379 / 27.51486 / 38.75712 / -11.2423
hsa-miR-218-1# / 27.58015 / 38.75712 / -11.177
hsa-miR-30a-3p / 27.6896 / 38.75712 / -11.0675
hsa-miR-1305 / 27.71055 / 38.75712 / -11.0466
hsa-miR-15b / 27.80883 / 38.75712 / -10.9483
hsa-miR-330-5p / 28.56167 / 38.75712 / -10.1955
hsa-miR-133b / 28.6739 / 38.75712 / -10.0832
hsa-miR-628-3p / 28.74788 / 38.75712 / -10.0092
hsa-miR-144# / 28.83036 / 38.75712 / -9.92676
hsa-let-7d / 26.73242 / 36.02627 / -9.29385
hsa-miR-302c / 29.51788 / 38.75712 / -9.23924
hsa-miR-377# / 29.65744 / 38.75712 / -9.09968
hsa-miR-539 / 29.69341 / 38.75712 / -9.06372
hsa-miR-15a# / 29.7067 / 38.75712 / -9.05043
hsa-miR-589 / 29.7596 / 38.75712 / -8.99752
hsa-miR-99a / 29.81754 / 38.75712 / -8.93959
hsa-miR-16-2# / 30.45328 / 38.75712 / -8.30384
hsa-miR-548G / 30.49524 / 38.75712 / -8.26188
hsa-miR-183 / 30.64012 / 38.75712 / -8.117
hsa-miR-145# / 30.65704 / 38.75712 / -8.10009
hsa-miR-132 / 30.68921 / 38.75712 / -8.06791
hsa-miR-370 / 30.69637 / 38.75712 / -8.06076
hsa-miR-95 / 30.71208 / 38.75712 / -8.04504
hsa-miR-136# / 30.71438 / 38.75712 / -8.04274
hsa-miR-625 / 30.74117 / 38.75712 / -8.01596
hsa-miR-148b / 30.77105 / 38.75712 / -7.98607
hsa-miR-214# / 31.59508 / 38.75712 / -7.16204
hsa-miR-582-3p / 31.62882 / 38.75712 / -7.1283
hsa-miR-339-5p / 31.6669 / 38.75712 / -7.09022
hsa-miR-340# / 31.67861 / 38.75712 / -7.07852
hsa-miR-361 / 31.70077 / 38.75712 / -7.05635
hsa-miR-603 / 31.70412 / 38.75712 / -7.053
hsa-miR-487b / 31.70705 / 38.75712 / -7.05007
hsa-miR-576-3p / 31.71087 / 38.75712 / -7.04625
hsa-miR-1244 / 31.71626 / 38.75712 / -7.04087
hsa-miR-213 / 31.76 / 38.75712 / -6.99712
hsa-miR-362 / 31.76184 / 38.75712 / -6.99528
hsa-miR-222# / 31.77401 / 38.75712 / -6.98311
hsa-miR-205 / 27.87677 / 34.76563 / -6.88886
hsa-miR-363 / 32.64032 / 38.75712 / -6.1168
hsa-miR-92a-1# / 32.68024 / 38.75712 / -6.07688
hsa-miR-654-3p / 32.68592 / 38.75712 / -6.0712
hsa-miR-376c / 32.75842 / 38.75712 / -5.99871
hsa-miR-335# / 32.7995 / 38.75712 / -5.95762
hsa-miR-532 / 33.64203 / 38.75712 / -5.1151
hsa-miR-27a# / 33.68351 / 38.75712 / -5.07361
hsa-miR-888 / 33.72916 / 38.75712 / -5.02797
hsa-miR-424# / 33.73022 / 38.75712 / -5.02691
hsa-miR-548J / 33.74674 / 38.75712 / -5.01039
hsa-miR-204 / 30.72133 / 25.64274 / 5.078583
hsa-miR-1183 / 30.64184 / 24.78054 / 5.861296
hsa-miR-193a-5p / 31.65958 / 25.78467 / 5.874907
hsa-miR-323-3p / 34.65065 / 28.74063 / 5.910025
hsa-miR-1267 / 27.69034 / 21.75241 / 5.937935
hsa-miR-545 / 33.69098 / 27.7526 / 5.938375
hsa-miR-1260 / 26.70261 / 20.73958 / 5.963029
hsa-miR-508 / 31.75892 / 25.7676 / 5.991325
hsa-miR-302a / 39.73606 / 33.60513 / 6.130925
hsa-miR-9# / 35.75344 / 28.87447 / 6.878976
hsa-miR-542-3p / 39.73606 / 32.80105 / 6.935009
hsa-miR-1289 / 39.73606 / 32.79256 / 6.943495
hsa-miR-548L / 39.73606 / 32.76498 / 6.971073
hsa-miR-433 / 35.73347 / 27.80042 / 7.933057
hsa-miR-548c-5p / 39.73606 / 31.73999 / 7.996065
hsa-miR-369-3p / 39.73606 / 30.73021 / 9.005852
hsa-miR-548d / 39.73606 / 30.72693 / 9.009126
hsa-miR-744# / 36.82548 / 27.81148 / 9.014001
hsa-miR-515-3p / 39.73606 / 29.85393 / 9.882125
hsa-miR-100# / 39.73606 / 29.80135 / 9.934709
hsa-miR-455 / 39.73606 / 29.79167 / 9.944389
hsa-miR-591 / 39.73606 / 29.79102 / 9.945034
hsa-miR-519d / 39.73606 / 29.74139 / 9.994663
hsa-miR-125a-3p / 39.73606 / 29.7138 / 10.02226
hsa-miR-653 / 39.73606 / 28.84072 / 10.89534
hsa-miR-488 / 39.73606 / 28.7493 / 10.98675
hsa-miR-518b / 39.73606 / 27.68547 / 12.05058
hsa-miR-34a / 39.73606 / 26.82449 / 12.91156
hsa-miR-1285 / 39.73606 / 25.69899 / 14.03706

Supplementary Figure 1. Overview of the design strategy.