Alignment of Zfh-1 family members. Below this is an alignment of the zinc finger regions of these proteins.

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

5 15 25 35 45 55

Human_Nil2 -----MADGP RCKRRKQANP RRNNVTNYNT VVETNSDSDD EDKLHIVEEE SVTDA---AD

Human_O60315 MKQPIMADGP RCKRRKQANP RRKNVVNYDN VVDTGSETDE EDKLHIAEDD GIANP---LD

Dros_Zfh1 ---MLSCLAP SSSRFGQEDT IIQQSMPSTS PFAMQFPSLA STLLHHNQSP KHSNPGSSGI

Celeg_F28F9.1 ------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

65 75 85 95 105 115

Human_Nil2 CEGVP------EDDLPTDQT VLPGRSSE-- -REGNAKNCW ED------DRKEGQ

Human_O60315 QETSPASVPN HESSPHVSQA LLPREEEEDE IREGGVEHPW HNNEILQASV DGPEEMKEDY

Dros_Zfh1 QDAHPNQPGA AADAFLVKCT QCHKRFPEYQ SLSEHIASEH PHDKLNCGAA QPESDAEDEQ

Celeg_F28F9.1 ------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

125 135 145 155 165 175

Human_Nil2 EILG------PEAQADEAG- -CTVKDDECE SDAEN------EQN-HDP NVEEFLQQQ-

Human_O60315 DTMG------PEATIQTAIN NGTVKNANCT SDFEEYFAKR KLEERDGHAV SIEEYLQRS-

Dros_Zfh1 SNMSGSSRRY AKSPLASNNN SSTANANNNS TSSQS--MNN NSELAKNHNS ANKMSPMCSP

Celeg_F28F9.1 ------MV-

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

185 195 205 215 225 235

Human_Nil2 ------DTAV IFPEAPEEDQ RQGTPEASGH DEN----GTP DAFS------QLLTCPYCDR

Human_O60315 ------DTAI IYPEAPEELS RLGTPEANGQ EENDLPPGTP DAFA------QLLTCPYCDR

Dros_Zfh1 GSLTPGDLFA QLQHPPPQLP PHLHAQFMAA AASLAMQSAR TASSPSQQQQ QQLQQQQQLQ

Celeg_F28F9.1 ------DIAE AMPTTASSLP ------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

245 255 265 275 285 295

Human_Nil2 GYKRFTSLKE HIKYRHEKNE DNFS------

Human_O60315 GYKRLTSLKE HIKYRHEKNE ENFS------

Dros_Zfh1 QQQQMQQQQQ HQMAMQQLLP PQLPGSNSSV GSNSAYDLDL SAPRSTSSPG STTGDLSGAY

Celeg_F28F9.1 ------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

305 315 325 335 345 355

Human_Nil2 -CSLCSYTFA YRTQLERHMT SHKSG------RD Q-RHV--TQS

Human_O60315 -CPLCSYTFA YRTQLERHMV THKPG------TD QHQML--TQG

Dros_Zfh1 PCMQCTASFA SREQLEQHEQ LHSPCGPAAV SNVSQTCRIC HKAFANVYRL QRHMISHDES

Celeg_F28F9.1 ------SD E------A-

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

365 375 385 395 405 415

Human_Nil2 GCNRKFKCTE CGKAFKYKHH LKEHLRIHSG EKPYECPNCK KRFSHSGSYS SHISSKKCIS

Human_O60315 AGNRKFKCTE CGKAFKYKHH LKEHLRIHSG EKPYECPNCK KRFSHSGSYS SHISSKKCIG

Dros_Zfh1 ALLRKFKCKE CDKAFKFKHH LKEHVRIHSG EKPFGCDNCG KRFSHSGSFS SHMTSKKCIS

Celeg_F28F9.1 --LRKFKCPE CTKAFKFKHH LKEHIRIHSG EKPFECQQCH KRFSHSGSYS SHMSSKKCVQ

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

425 435 445 455 465 475

Human_Nil2 LIPVNGRPRT GLKTSQCSSP SLSASPGSPT RPQIRQKIEN -K--PLQEQL SVNQIKTEPV

Human_O60315 LISVNGRMRN NIKTGSSPN- SVSSSPTNSA ITQLRNKLEN GKPLSMSEQT GLLKIKTEPL

Dros_Zfh1 MGLKLNNNRA LLKR------LEKSPGSAS SASRRSPSDH GKG-KLPEQP SLPGLPHPMS

Celeg_F28F9.1 QASPS------MVTPFN-- -PYQLMMYRN -----IMLQL QTPQVSFLPS

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

485 495 505 515 525 535

Human_Nil2 DY-EFKPIVV ASGINCSTPL QNGVFTGGGP LQATS---SP QGMVQAVVLP TVGLVSPISI

Human_O60315 DFNDYKVLMA THGFSGTSPF MNGGLGATSP LGVHPSAQSP MQHLGVGMEA PLLGFPTMNS

Dros_Zfh1 YFASDAQVQG GSAAPAPFPP FHPNYMNAAL LAFPHNFMAA AAGLDPRVHP YSIQRLLQLS

Celeg_F28F9.1 TAANNMDYMS LLQANLFQSL ENGTSP--TP TQEPSAPASP EPKIEVVDEP -----EVSSE

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

545 555 565 575 585 595

Human_Nil2 NLSDIQNVLK VAVDGNVIRQ VLE---NNQA NLASKEQETI NASPIQQGGH S-VISAISLP

Human_O60315 NLSEVQKVLQ IVDN-TVSRQ KMDCKAEEIS KLKGYHMKDP CSQPEEQGVT SPNIPPVGLP

Dros_Zfh1 AAGQQQREEE REEQ--QKQQ QHD---EEET PDEPKLVMDI EEPETKEMAP TPEATEAATP

Celeg_F28F9.1 VKTEVKTEVK TEDS------VPE---ESIT PAVS---MSL SPAPEQNGNE S--MNNGGS-

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

605 615 625 635 645 655

Human_Nil2 LVDQDGTTKI IINYSLEQPS QLQVVPQNLK KENPVATNSC KSEKLPEDLT VKSEKDKSFE

Human_O60315 VVSHNGATKS IIDYTLEKVN EAKACLQSLT TDSRRQISNI KKEKLRTLID LVTD-DKMIE

Dros_Zfh1 IKREESR-EA SPDPESYRSS S-----QAIK QEQEPLNVAE ERQ------TPVEEHAPVE

Celeg_F28F9.1 --GSDG--KS SPDWRPLRS------RSFL NDSQVAVLQN ------HFK

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

665 675 685 695 705 715

Human_Nil2 GG--VNDSTC LLCDD-CPGD INALPELKHY DLKQPTQPPP LPAAEAEKPE SS------

Human_O60315 NHNISTPFSC QFCKESFPGP IPLHQHERYL CKMNEEIKAV LQPHENIVPN KAGVFVDNKA

Dros_Zfh1 H---SADLRC SRCSKQFNHP TELVQHEKVL CGLIKEELEQ HFQ-QQQATS FA------

Celeg_F28F9.1 ----RNPFPS KYELSAVAEQ IGVNKRVVQV WFQNTRAKER RSNRLPSMPR GS------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

725 735 745 755 765 775

Human_Nil2 --VSSATGDG NLSPSQPPLK NLLSLLKAYY ALNAQPSAEE LSKIADSVNL PLDVVKKWFE

Human_O60315 LLLSSVLSEK GMTSPINPYK DHMSVLKAYY AMNMEPNSDE LLKISIAVGL PQEFVKEWFE

Dros_Zfh1 --LASASEED EEDEE------MDVEEE PRQESGERKV

Celeg_F28F9.1 --VASAAAAA ATSPT------VWQT PVQLMAAWAS

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

785 795 805 815 825 835

Human_Nil2 KMQAGQISVQ SS---EPSS- PEPGKVNIPA KNNDQPQS------AN ANEPQDSTVN

Human_O60315 QRKVYQYSNS RSPSLERSSK PLAPNSNPPT KDSLLPRSPV KPMDSITSPS IAELHNSVTN

Dros_Zfh1 RVRTAINEEQ QQ------QLKQHYS------LN ARPSRDEFRM

Celeg_F28F9.1 QFSNGNNSLT AS------QDER------N N-ENTDEVMD

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

845 855 865 875 885 895

Human_Nil2 LQSPLKMTN- ---SPVLPVG STTNGSRSST PSPSPLNLSS SRNTQGYLYT AEGAQ-EEPQ

Human_O60315 CDPPLRLTKP SHFTNIKPVE KLDHS-RSNT PSPLNLSSTS SKNSHSSSYT PNSFSSEELQ

Dros_Zfh1 IAARLQLDP- ----RVVQVW FQNNRSRERK MQSFQNNQAA GAAPPMPIDS QASLT---RE

Celeg_F28F9.1 HDGLKDGKE- ---TPLDLTL STDDTEPEWS PEKLIGFLDQ TGGVIQELLR QAGNG--FVT

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

905 915 925 935 945 955

Human_Nil2 VEPLDLSLPK QQGE------LLERSTIT SVYQNSVYSV QE---EPLNL SCAKKEPQKD

Human_O60315 AEPLDLSLPK QMKEPKSIIA TKNKTKASSI SLDHNSVSSS SENSDEPLNL TFIKKEFSNS

Dros_Zfh1 DQPLDLSVKR DPLTP------KSESSPP YIAPPSGEAL NP---EAINL SRKFSTSASM

Celeg_F28F9.1 NQEDEEEKPI KAEE------SPVSSGSS SIWPSFIGQY PS---ILDSA SLSVLEKALD

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

965 975 985 995 1005 1015

Human_Nil2 SCVTD--SEP VVNVIPPSAN PINIAIPTVT AQLPTIVAIA DQNSVPCLRA LAANKQTILI

Human_O60315 NNLDNKSTNP VFSMNPFSAK PLYTALPPQS AFPPATFMPP VQTSIPGLRP YPGLDQMSFL

Dros_Zfh1 SPASIS--PS SAAALYFGAA PPPSPPNSQL DSTPRSGQAF PGLPPYMLPM SLPMEALFKM

Celeg_F28F9.1 VSFID---FS ECFAIQFLML HVLNSVVSWK EQKTFLAYLS GKN----FFD SLGFREDIRA

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

1025 1035 1045 1055 1065 1075

Human_Nil2 PQVAYTYSTT -VSPAVQEPP LKVIQ-PNGN QDERQDTSSE GVSNVEDQND SDSTPPKKKM

Human_O60315 PHMAYTYPTG -AATFADMQQ RRKYQRKQGF QGELLDGAQD YMSGLDDMTD SDSCLSRKKI

Dros_Zfh1 RPGGDFASNH ALMNSIKLPD YRGTSLSPGG SEKRSWRDDD SRISHEDEFG AGVLMPPKPR

Celeg_F28F9.1 VRYVIIYSNK QIFEKLYYPP RYALT--NNF QDMQQKSSED DASSLCS-NE SKLLKFPTTP

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

1085 1095 1105 1115 1125 1135

Human_Nil2 RK-----TEN G----MYACD LCDKIFQKSS SLLRHKYEHT GKRPHECGIC KKAFKHKHHL

Human_O60315 KK-----TES G----MYACD LCDKTFQKSS SLLRHKYEHT GKRPHQCQIC KKAFKHKHHL

Dros_Zfh1 RGKVETHGHA GDPDLPYVCD QCDKAFAKQS SLARHKYEHS GQRPYQCIEC PKAFKHKHHL

Celeg_F28F9.1 LK-----EEE G----LFSCD QCDKVFGKQS SLARHKYEHS GQRPYKCDIC EKAFKHKHHL

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

1145 1155 1165 1175 1185 1195

Human_Nil2 IEHMRLHSGE KPYQCDKCGK RFSHSGSYSQ HMNHRYSYCK REAEERDSTE QEE------A

Human_O60315 IEHSRLHSGE KPYQCDKCGK RFSHSGSYSQ HMNHRYSYCK REAEEREAAE REAREKGHLE

Dros_Zfh1 TEHKRLHSGE KPFQCSKCLK RFSHSGSYSQ HMNHRYSYCK PYRE------

Celeg_F28F9.1 TEHKRLHSGE KPFQCDKCLK RFSHSGSYSQ HMNHRYSYCK PYRE------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

1205 1215 1225 1235 1245 1255

Human_Nil2 GPEILSNEHV GARASPS-QG DSDERESLTR EEDEDSEKEE EEEDKEMEEL QEEKECEKPQ

Human_O60315 PTELLMNRAY LQSITPQGYS DSEERESMPR --DGESEKEH E---KEGEDG --YGKLGRQD

Dros_Zfh1 ------

Celeg_F28F9.1 ------QPASPS--- DVLNGGSVTV S------PS ------

....|....| ....|....| ....|....| ....|....| ....|....| ....|....|

1265 1275 1285 1295 1305 1315

Human_Nil2 GDEEEEEEEE EVEEEEVEEA ENEGEEAKTE GLMKDDRAES QASSLGQKVG ESSEQVSEEK

Human_O60315 GDEEFEEEEE ESENK---SM DTDPETIRDE EETGDHSMDD SSEDGKMETK SDHEEDNMED

Dros_Zfh1 ------

Celeg_F28F9.1 ------SS NTPPPST------

....

Human_Nil2 TNEA

Human_O60315 GM--

Dros_Zfh1 ----

Celeg_F28F9.1 ----

Alignment of the zinc finger regions of the above proteins.

NIL2_HUMAN CNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCI

O60315 GNRKFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCI

F28F9.1 ALRKFKCPECTKAFKFKHHLKEHIRIHSGEKPFECQQCHKRFSHSGSYSSHMSSKKCV

zfh-1 LLRKFKCKECDKAFKFKHHLKEHVRIHSGEKPFGCDNCGKRFSHSGSFSSHMTSKKCI

***** ** ****:*******:********: * :* ********:***::****:

NIL2_HUMAN MYACDLCDKIFQKSSSLLRHKYEHTGKRPHECGICKKAFKHKHHL

O60315 MYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHL

F28F9.1 LFSCDQCDKVFGKQSSLARHKYEHSGQRPYKCDICEKAFKHKHHL

zfh-1 PYVCDQCDKAFAKQSSLARHKYEHSGQRPYQCIECPKAFKHKHHL

: ** *** * *.*** ******:*:**::* * *********

NIL2_HUMAN IEHMRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAE

O60315 IEHSRLHSGEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAE

F28F9.1 TEHKRLHSGEKPFQCDKCLKRFSHSGSYSQHMNHRYSYCKPYRE

zfh-1 TEHKRLHSGEKPFQCSKCLKRFSHSGSYSQHMNHRYSYCKPYRE

** ********:**.** ********************* *