HTSF Instrumentation for Users
EnVision® Multilabel Plate Reader
Model 2104-0020
Technologies
The system reads all non-isotopic detection technologies in all major applications, including:
•Top and Bottom Read of Fluorescence
•Luminescence
•Absorbance
Applications
EnVision is a multilabel reader for all major applications, including
- Reporter gene assays
- Cell applications
- Genotyping
- Enzyme assays
- Signal transduction
- Receptor ligand binding
- Immunoassays
- Quantification
- Possible other future assays.
Counting modes and features included
EnVision counting modes are easily accessible. In the same protocol, even for the same well, you can freely use several different counting modes and labels.
Plate format:
96-well; 384-well; 1536-well
Temperature Control
To ensure reproducibility of cellular and enzyme kinetic assays and other assays that require a defined temperature range, the EnVision platform’s temperature control option lets you precisely regulate from +2°C to +45°C above ambient. Temperature control is uniform across the plate, and a top heating plate eliminates condensation problems.
Dispenser
The EnVision dispenser can be used with 1- to 384-well plates in all reading modes. It consists of two pump units, a magnetic stirrer and a heater, allowing precise delivery of reagents in 1 μL increments over a volume range of 2-475 μL. An adjustable dispense speed setting lets you optimize for viscosity, cell density and other assay requirements.
Plate Stacker
Available for 20 or 50 plates, interchangeable plate stacker magazines can easily be added to increase capacity and throughput. For kinetic assays, the grip-fed re-stacking function lets you maintain the order of plates in the stack, increasing the reliability of data.
Plate barcode
The barcode reader allows you to loadbarcode labeled plates, which areidentified by the barcode reader.Codabar, Code39, Interleaved 2 of 5,Code 128, UPC and EAN barcodes canbe read.
Plate loading modes
Available in HTSF:
Manual reading (one plate at time)
Stacker loading (up to 50 plates per load)
Optical filters
A wide range of filters cover the wavelengths used in fluorometric and photometric applications. Below are 16 filters available in HTSF:
Barcode / 217 / 243 / 229 / 214 / 207 / 206 / 102 / 319Wavelength / M590 / M685 / M595w / M510 / M460 / M535 / X485 / P600
Barcode / 103 / 318 / 105 / 302 / 227 / 112 / 118 / 303
Wavelength / X355 / P570 / X531 / P405 / M650 / X380 / X620 / P450
Application-specific mirror modules
User interchangeable application specific optical modules form a crucial part of the optical system. The optical modules include all application critical components; for example dichroicmirrors for specific labels. All application-specific optical modules are barcoded.
Top and bottom reading
Fluorescence readings (including FP and TRF) can be taken from the top or bottom. Top reading is the most efficientmethod when no lid is used because no plastic surface has to be penetrated. For adherent cells and lidded plates, belowreading provides superior efficiency. It is a true epimode, in the sense that the both excitation and emission are directed to and from below.For top reading, four optical modules can be in place. Any of the them can also be used for below reading.
Below are 5 mirror modules available in HTSF.
Barcode / 401 / 403 / 405 / 412 / 420Wavelength? / B550 / D505 / D555 / D400 / D658
Ultra-Sensitive Luminescence technology makes the most of your precious cells and reagents
For the ultra sensitive luminescence, we have 5 types apertures (96-L1, 384-L1,1536-L1,1536-LP-L1 and 0L1/A1) for 96, 384 ad 1536 plates respectively. They areworking with primary, stem or difficult-to-transfect cells presents a unique set of challenges. Optional ultra- sensitive luminescence boosts sensitivity by almost 40X vs. standard luminescence by bringing the detector closer to the sample—giving you more information from every cell.
Software that enhances both productivity and compliance
Powerful software makes it easy to take advantage of all the performance and versatility of the EnVision platform. An intuitive interface with pre-set user-specific protocols makes setting up the system fast and easy. For post-run data analysis, features such as automatic curve fitting and flexible interpretation of standards controls and unknowns gives the EnVision system the power to handle a broad range of assay types. The software integrates smoothly into your lab’s network and into lab automation and data management systems. For regulated environments, PerkinElmer’s Enhanced Security mode provides technological controls and features that support 21 CFR Part 11 compliance.
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