How to Stitch Images Using Autopano Giga

How to Stitch Images Using Autopano Giga

1. Download JPGs from server
2. Rename the folder so that it has the same name as the organism.
3. Ex. Downloaded organism is the earthworm. Rename the folder, which downloads as ‘jpg’, ‘Earthworm’. (This will organism will be used throughout the directions)
4. Create a new file on the desktop named as Organism Cropped (should be same organism in (2)).
5. Ex. Earthworm Cropped
6. Go into “Saved Panos” (located on the desktop) and create new folder with the organism’s name. This is where the stitched image will go before it has been processed. It serves as a back-up so that if something goes wrong later, you will not have to go through the process of stitching it again and can just make changes from this file.
7. Ex. Earthworm
8. Open Adobe Photoshop CS5
9. In Photoshop:
10. Click File (on the top-most bar, the grey bar)
11. Then Automate (on the dropdown list)
12. Then Batch (appears when hover over Automate)
13. In the Batch window:
14. Change action to Crop Images
15. Choose the source folder to be the folder with the organism’s name, which is located on the desktop (I’d select Earthworm—folder from (2))
16. Choose the destination folder to be Organism Cropped (I’d select Earthworm Cropped—folder from (3))
17. Ensure ‘Override Action “Save As” command is checked
18. Click ok
19. When Batch is done copying, open the Organism Cropped file to ensure that the images are there
20. Open AutoPano Giga
21. Click Autopano Giga (on the top-most bar) and in the drop down menu hit Preferences
22. In the multiple tabs that appear, choose the Detection tab.
23. In the Detection tab:
24. Choose the baseline settings:
25. Standard
26. 51 control points
27. Ransac model: simliarity
28. Unclick auto color correction
29. In the Optimization tab:
30. Choose the baseline settings:
31. If less than 100 images per layer  Strong. If more than 100 images per layer Gigapixel
32. Multiple viewpoints is checked
33. Lens distortion is checked
34. In the Render tab:
35. Choose the baseline settings:
36. Remove ghost is unchecked
37. Choose JGPs from the dropdown menu, if it is not chosen already
38. Quality: 10
39. Change the destination folder to be Layers for the appropriate organism. To find this folder, go into the server (mvrlnas2). Select BioGigaImages. Go to the appropriate organism’s folder (I’d click on Earthworm) and put it in the folder entitled ‘Layers’.
40. Hit Ok. You have now just adjusted the default settings. Most of the time, the images will stitch without any problems with these configurations.
41. Click File (on top)
42. Select images
43. Select images from Organism Cropped (Earthworm Cropped) folder.
44. Select all of the pictures that comprise one layer (a layer is defined by the black frames. Choose all of the pictures between those frames)
45. Repeat (17) until all layers have been chosen
46. On the bottom, next to ‘All Groups’, click the “Detect All” button,
47. If images appear fine: Congrats! You don’t have to do any intensive editing. If editing needs to be done, go to the ‘Editing Stitched Layers’ section; if not, continue along.
48. Hit Edit Panorama
49. Click ‘Save Project’.
50. Save it to the folder from (4).
51. Name it x layer, where x corresponds to what layer it is numerically
52. Ex. If it’s the 23rd layer, call it 23rd layer.
53. Click Render and then hit render on the window that popped up
54. Back in the editor window, click View (on top)
55. Then click Layers: a window with all the selected images should appear
56. In this window, expand the window so that the entire name appears.
57. Take a screenshot of this window.
58. Go back to the desktop, the screenshot should appear.
59. Name it the same as in (22b)
60. Update the textfile
61. In the textfile, report all of the settings used.
62. Update Twiki