Additional file 2

10 20 30 40 50 60 70 80 90

CheSox12 ------MEVD------VGMDFFLCETL------PTPEPQDGDLNMMQHL------

HmaSox4 ------MELD------FGLDYFMSGAL------PTPEPQDGDI---KHL------

NveSoxC ------MMMVLQNSEF----RASPSPPHSLS------

AmiSoxC ------MMVFHGSEL-----NSPSPPPQV------

PpiSOX12 ------

HSASox4 MVQQTNNAENTEALL------AGESSDSGAGLELGIASSPTP------

HSASox11 MVQQAESLEAESNLP------REALDTEEGEF------

HSASox12 MVQQ------RGARAKRDGGP------PPPGP------

DmeSoxC MIAKPNQATTEPPLSLRPGTVPTVPATTPARPATITIQRRHPAPKADSTPHTLPPFSPSPSPASSPSPAPAQTPGAQKTQSQAAITHPAA

CinSoxC MASTSRLTKISAELM------SLHAGQNLDGNPGSPESGLGSDWDFDSDEIVQSSSVATPRRSIDALHASSPSPTLECILDCPDVSGVDV

CelSoxC ------MDLQ------KPPNFMLDCGM--APHMMPPINWAAAAIAVASSTSGATNATSSNSVATSQQ

AquSoxC ------MSAGDLQ------YTVILQQAPSPSCSSLSSPSSPYQSNLLDVCD------

100 110 120 130 140 150 160 170 180

CheSox12 ------NPLELSVGDSNSQRPIK------RKKSGN

HmaSox4 ------NPLELNI---DFQLGCG------NKKKSS

NveSoxC ------GGMDDQVDATQATHESK------KKSDM-

AmiSoxC ------PSVDEAE---EEPKKSM------Q-----

PpiSOX12 ------

HSASox4 ------GSTASTGGKADDPSWCK------TPS---

HSASox11 ------MACSPVALDESDPDWCK------TAS---

HSASox12 ------GPAEEGA---REPGWCK------TPS---

DmeSoxC VASPSAPVAAASTEDPQDPGPRSTHTHTHSQHFSPPPRESEMDGERSPSHSGHEMTLSMDGIDSSLVFGSARVPVNSSTPYSDATRTKKH

CinSoxC FDSPIKKEADFKDPKPRQSPNKNKRENILNELKNLPATKIKL---PPPSRSS------QKKLAA

CelSoxC LQHHPYGTAAGGYKHAQ------QAIPKSVTPYSDATNCK------KSS---

AquSoxC ------VGTATHVHHNGGSRGGG------NGGSAG

190 200 210 220 230 240 250 260 270

CheSox12 MTDHVKRPMNAFMVWSQIERKKMAESYPDMHNAEISRRLGKQWKMLTDDDRRPYVIRSEKLREEHMRRHPDYKYRPKKKAK------

HmaSox4 M-DHVKRPMNAFMVWSQIERKKMADIYPDMHNAEISRRLGKRWKLLSDADRRPFVIRSEKLREEHMRRYPDYKYRPKKKAK------

NveSoxC --QHVKRPMNAFMVWSQIERRKMAEEHPDMHNAEISKRLGKRWKLLSESEKRPFVEESERLRIRHMQAYPDYKYRPRKKKQ------P

AmiSoxC ---HVKRPMNAFMVWSQIERRKMAEEHPDMHNAEISKRLGKRWKLLSESEKRPFVEESERLRIRHMQAYPDYKYRPRKKKQ------P

PpiSOX12 ------VWSQIQRAKIVEEQPNKHNAAISKQLGSEWKMLSDEARMPYIHESQRLKRIHKQQYPDYKYRPRKRGK------G

HSASox4 --GHIKRPMNAFMVWSQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGNANSSSS

HSASox11 --GHIKRPMNAFMVWSKIERRKIMEQSPDMHNAEISKRLGKRWKMLKDSEKIPFIREAERLRLKHMADYPDYKYRPRKKPK------MDP

HSASox12 --GHIKRPMNAFMVWSQHERRKIMDQWPDMHNAEISKRLGRRWQLLQDSEKIPFVREAERLRLKHMADYPDYKYRPRKKSK------GAP

DmeSoxC SPGHIKRPMNAFMVWSQMERRKICERTPDLHNAEISKELGRRWQLLSKDDKQPYIIEAEKLRKLHMIEYPNYKYRPQKKQT------RSP

CinSoxC RPGYIKRPMNAFMIWSQIERRKIMEKTPELHNAEISRNLGRIWREQADSIKRPFLIEAERLRLQHMCDYPDYKYKPKKKAK------

CelSoxC --NHIKRPMNAFMVWSQMERRKICEHQPDMHNAEISKQLGSRWRSLTDEEKAPFVAEAERLRVCHMQEYPDYKYKPRKKPK------KNP

AquSoxC KKEHIKRPMNAFMVWAQLERRKMTTEFPDMHNAEISRRLGKLWRLLSDREKQPYIEESERLRIQHMKQYPDYKYRPRKKGG------KKP

280 290 300 310 320 330 340 350 360

CheSox12 ------EMEGKQGAATQTNQQRANNTKNN------

HmaSox4 ------ELAAKELAAKELKNFSKVCEYNIVTLTNGRNDD------W------

NveSoxC AKAKP------GDAKPAASEQS------PRKNLTVTALGTKRE------

AmiSoxC AKQKN------GGAQDSKTSSSNSDGHSHSRKHIAETVGTKRE------

PpiSOX12 TTTTTTGVTTKENTAPSVNITRXGPVQLPPRSTTPDVHHSKPQTTPRHQAVIVREQRSTA------SYTVPFIK------

HSASox4 AAASSKPGEKGDKVGGSGGGGHGGGGGGGSSNAGGGGGGASGGGANSKPAQKKSCGSKVAGGAGGGVSKPHAKLILA------

HSASox11 S-AKPSASQSPEKSAAGGGGGSAGGGAGGAKTSKGSSKKCGKLKAPAAAGAKAGAGKAAQSGDYGGAGDDYVLGSLRVSGSGGGGAGKTV

HSASox12 AKARP------RPPGGSGGGSRLKPGPQLPGRGGRRAAGGPLGGGAAAPE------

DmeSoxC GSLKP------NQDADGCEARNDTTNNNNSLTTLAINGTTTAGRKSK------

CinSoxC ------GKKCDSSENSTFSYLHNDPDQSMEIIETNTEAL------

CelSoxC DGTLQ------QPAQPQAPQQQQAPPRGASPQARQRKRPNTDQQ------S------

AquSoxC KPVSN------TSYLGGNDSGSEEYYPTTMPTSSSNSCSCGAGIRRA------PVPTCSIAVQ------

370 380 390 400 410 420 430 440 450

CheSox12 ------TNTLLAIEGNS------~~~~~~YDSTMIKLGPSTTLAYNALNSKHLL------

HmaSox4 ------DKSKYTNNVACCENNKAPPNKT------FVTISRQVGQIGSLSNITVSPQKCIDIPPS---

NveSoxC ------ALPGAQMGS------YYG-SSSAKKFNSMSEPTYKKQRRDLG-P----

AmiSoxC ------ALPGAH------GYPGLGSSKKQATSHGEAPFKKQRRDFASP----

PpiSOX12 ------RKRTLKIERVTEEAAGRFEFSEPAVKKRPTSVKRF--VAVQLDSSKSTTGQGGVKRFRILTTTSNNT

HSASox4 ------GGGGGGKAAAAAAASFAAEQAGAAALLPLGAAADHHSLYKARTPSASASASSAASASAALAAPGKHL

HSASox11 KCVFLDEDDDDDDDDDELQLQIKQEPDEEDEEPPHQQLLQPPGQQPSQLLRRYNVAKVPASPTLSSSAESPEGASLYDEVRAGATSGAGG

HSASox12 ------DDDEDDDEELLEVRLVETPGRELWR------MVPAGRAARGQAERAQGPSGEGAAAAAA------

DmeSoxC ------RSTSTCQSGSA------SKRLRNDSGDTSSKPKYEVKMESAEQL---

CinSoxC ------KAEVKVSSKRLPKKRKLSQSKPSLPEIKPTVVASINPIQNNSGNSEPQAKEVTATTNNLL

CelSoxC ------ETQQFQNFKSVKVEQDWMGNAHMSHAQKMPFHPSYPS-PSEFGHAPLTPESGFYDDYFTQQHHQQHF

AquSoxC ------CSMELGEHVIEREPSSPKQTAEISI---QVGNGSAHLQQNRRRSFSSFAGDKRPRDLSLSCPPIKKR

460 470 480 490 500 510 520 530 540

CheSox12 ------KPIKQANTTNAANVPLQRIESAKLVNIKNGQC----VMTTGKPFLTLSRQVGKIGS~~~LSSSEGNFSISPQKNSLL

HmaSox4 ------TLSPPSVVPDCCGTPPDEYNSESFYNYDSFSG------KNNSNS------

NveSoxC ------ITPPPNVPDSIGVTPEEPIDQLSLYEDFDQAF------KTEQNA------QG----INSNHQQAAHNNHQIPSG

AmiSoxC ------ITPPPNVPDAIGVTPDDTIDQLSLYEDFEHAF------KQDQQV------PS----INHT------QQMPNS

PpiSOX12 TTNLFNNQ-----KQPFTLTPPSSLALSSQPSKRPRLVDCSPALP------HEREIIKELRGGGY----LSQTCGSTMKSEDILSSK

HSASox4 AEKKVKRVYLFG-GLGTSSSPVGGVGAGADPSDPLGLYEEEGAGCSPDAPSLSGRSSAASSPAAGRSP----ADHRGYASLRAASPAPSS

HSASox11 GSRLYYSFKNITKQHPPPLAQPALSPASSRSVSTSSSSSSGSSSG------SSGEDADDLMFDLS----LNFSQSAHSASEQQLGGG

HSASox12 ------ASPTPS------EDEEPEEEEEEAAAA------EEGEEETVASGEES----LGFL------SRLPPG

DmeSoxC ------NSADIILPSADNLISYQSSEYLPLSTLSNADC------DEKLHSELSSGP----LESRENLSEVVNRFLPLF

CinSoxC TAVKVE------PRVPPLPITPATIVTAVPSKRGRFEVHTPSPC------DTNQRIGLVHGTVFKTVTEGNRQFIIVSGANDVISD

CelSoxC ASQ------HHNSAGSPLRMTNLGMDMGMPPQMMGHNSGFG------AGNHPFYLHTSPPS----VDQDDMRSLSSGSSGYAD

AquSoxC ------ALIPVSSPPEVLPTSLDDIFPPSPPSSDSSPI------SHQHREDTLPTLHF----EEFMDPLMPYDPSAVDLA

550 560 570 580 590 600 610 620 630

CheSox12 K------

HmaSox4 ------LYHEDMDLLINFARFT------DGLPDLYTTPEVSEMLTGQW

NveSoxC WPNLELST------LNLNSLPVSPLFGLDG------ANGGQFDFPDLYTPPEVSELIQGQW

AmiSoxC WPNLEFGN------MNFNGLPVSPLLDLHG------TSAGQFDFPDLYTPPEVSELIQGQW

PpiSOX12 VQ------LDNLYFDSHTDYFSGS------LNEKDLSLSD-YTTEEVWNLIRVE-

HSASox4 APSHASSSASSHSSSSSSSGSSSSDDEFEDDLLDLNPSSNFESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPD-YCTPEVSEMISGDW

HSASox11 AAAGN------LSLSLVDKDLDSFSEG------SLGSHFEFPD-YCTPELSEMIAGDW

HSASox12 PAG------LDCSALDRDPDLQPP------SGTSHFEFPD-YCTPEVTEMIAGDW

DmeSoxC LGGNEDSQ------LGVSSLTQSQHNQSDP------TAGLMDNISDISPINDREELTEEVM

CinSoxC VTNSSLVSTQRTSTPCQQKQI------INANFVPITNDCGQEKVYN------IVRGCIQVASQEPTNSTTQKRVEPD

CelSoxC CSASEQST------SSPNSAGVVTMATAAT------TTTHLDDLEQICPTVTTGELVNYPW

AquSoxC LSGSSSAL------SSSTAASNVLSLNTFPLQSSAATSIFSPFNIDSCSTFDFPEL--PSDFADIFAQNA

640

CheSox12 ------

HmaSox4 LENDLGF------

NveSoxC LENSLDQL------

AmiSoxC LENSLGHL------

PpiSOX12 APFTTTALTCCSCKILMF

HSASox4 LESSISNLVFTY------

HSASox11 LEANFSDLVFTY------

HSASox12 RPSSIADLVFTY------

DmeSoxC VPALPGGESIERRAHP--

CinSoxC VPTTFANMGDDFRNEYLK

CelSoxC SDALGIDINFS------

AquSoxC SEFDTSITTLLST-----