S1 Parameter estimates and associated statistics of the linearized carapace length-wet weight function for all developmental stage in both beaches

Developmental stage / Reflective beach
log a / b / r2 / P
Mean (±SE) / Mean (±SE)
Megalopae / -5.32 (0.41) *** / 0.80 (0.30) * / 0.01 / <0.05
Early females / 0.04 / ns
Neotenic males / -6.44 (0.49) *** / 1.62 (0.37) *** / 0.32 / <0.01
Juveniles / -7.42 (0.53) *** / 2.46 (0.32) *** / 0.85 / <0.01
Males / -8.56 (0.36) *** / 3.02 (0.21) *** / 0.66 / <0.01
Males with spermatophores / -8.58 (0.27) *** / 3.13 (0.14) *** / 0.91 / <0.01
Females / -8.72 (0.25) *** / 3.18 (0.12) *** / 0.93 / <0.01
Dissipative beach
log a / b / r2 / P
Mean (±SE) / Mean (±SE)
Megalopae / -5.18 (0.22) *** / 0.73 (0.16) ** / 0.11 / <0.01
Early females / 0.01 / ns
Neotenic males / -5.85 (0.38) *** / 1.09 (0.28) ** / 0.13 / <0.01
Juveniles / -7.81 (0.33) *** / 2.61 (0.18) *** / 0.55 / <0.01
Males / -7.30 (0.22) *** / 2.14 (0.13) *** / 0.35 / <0.01
Males with spermatophores / -7.73 (0.16) *** / 2.63 (0.09) *** / 0.68 / <0.01
Females / -8.23 (0.09) *** / 2.97 (0.04) *** / 0.89 / <0.01
Ovigerous females / -7.03 (0.62) *** / 2.63 (0.21) *** / 0.74 / <0.01

log a intercept, b slope, SE standard error, ns P>0.05, * P<0.05, ** P<0.01, ***P<0.01


S2 Description of oogenesis stages of Emerita brasiliensis

Histological analysis validated the macroscopic categorization of gonads, showing five oogenesis stages in E. brasiliensis in both beaches: oogonia, early vitellogenic oocyte, vitellogenic oocyte, mature oocyte and oosorption oocyte. Oogonia were grouped in nests, showed scarce or null cytoplasm surrounding the basophilic nucleus (diameter 10-15 um) (S 3a). These cells occurred in all ovarian stages. Early vitellogenic oocytes were also grouped in nests, had a larger size than oogonial cells (diameter 15-40 um) and basophilic cytoplasm (S 3b). These cells were presented in the ovarian stages II and III. Vitellogenic oocytes (diameter 40-80 um) showed a cytoplasm vacuolization and deposits of yolk near the plasmatic membrane during primary vitellogenesis (S 3c). In the following stage (secondary vitellogenesis), these oocyte displayed acidophilic plaques, which lipid vacuoles displaced towards to the cell centre, reaching larger sizes (diameter 80-150 um) (S 3d). Vitellogenic oocytes were present in ovarian stage II. Mature oocytes reached the largest sizes (diameter 150-330 um) and showed a complete vitellogenesis, occurring only in ovarian stage III (S 3e). Ovaries of E. brasiliensis were not empty thoroughly during ovulation, containing a number of unspawned relict mature oocytes, which never reached the exterior and were resorbed (oosorption). These oocytes showed vacuoles in a disintegrative process and membrane rupture (S 3f) and were registered in the ovarian stage IV and II (the latter only when the previous reproductive events have been occurred). No differences were found between beaches in the size of oocytes for all the five stages.


S3 Emerita brasiliensis. Microphotography of oocyte stages: (a) oogonia (40X), (b) early vitellogenic oocytes (40X) (c) y (d) vitellogenic oocytes, (e) mature oocytes (20x), (f) oosorption (40X). oG, oogonia; oS, early vitellogenic oocyte; oV, vitellogenic oocyte; oM, mature oocyte; cf, follicular cell.