Supplementary Fig. S1. Phylogenetic trees, based on maximum-parsimony (A)and maximum-likelihood (B) analysis of the 16S rRNA gene sequences, showing the position of strain TRM 4064T and closely related species. Sequence accession numbers are shown in parentheses. Bootstrap values are based on 1000 replicates; Bootstrap values are indicated at branch-points. Rubrobacter radiotolerans DSM 46359Twas used as this outgroup.
Supplementary Table S1. Cultural characteristics of strain Differential characteristics of strain A.dayingensis TRM 4064T,A.xinjiangensis TRM 40136T, A.mortivallis DSM 44261T and A. halophilaJCM 3278Ton various media at 37°C.
Media / A.dayingensisTRM 4064T / A.mortivallis DSM 44261T / A.xinjiangensis TRM 40136T / A. halophila JCM 3278TCzapek agar
Growth / Good / Good / Moderate / Good
Aerial mycelium / Yellow-white / Light-yellow / Yellow-white / -
Substrate mycelium / Yellowish brown / Brown / Yellow-white / Yellow-white
Soluble pigment / brown / Yellow / - / -
Glycerol-asparagineagar (ISP 5)
Growth / Weak / Moderate / Good / Moderate
Aerial mycelium / White / - / Pale yellow / -
Substrate mycelium / Pale yellow / Gray yellow / Yellow-white / Yellow-white
Soluble pigment / Yellow / Yellow / - / -
Inorganic salts
starch agar (ISP 4)
Growth / Good / Good / Good / Good
Aerial mycelium / Yellow-white / Yellow-white / Yellow / Yellow-white
Substrate mycelium / Pale yellow / Yellow / Yellow-white / Yellow-white
Soluble pigment / Yellowish green / - / - / -
Yeast extract-malt
extract (ISP 2)
Growth / Moderate / Moderate / Weak / Moderate
Aerial mycelium / White / Yellow-white / - / Yellow-white
Substrate mycelium / Brown / Brown / Brown / Yellow
Soluble pigment / - / - / - / -
Oatmeal agar
(ISP 3)
Growth / Weak / Moderate / Moderate / None
Aerial mycelium / White / Yellow / White / -
Substrate mycelium / Pale yellow / Light yellow / Brown / -
Soluble pigment / Yellowish green / - / - / -
Nutrient agar
Growth / Moderate / Moderate / Moderate / Moderate
Aerial mycelium / White / Yellow-white / Yellow-white / -
Substrate mycelium / Brown / Brown / Pale yellow / Yellow
Soluble pigment / Brown / Yellow / - / -
Note: Tested media containing 13% (w/v) salt; pH 7.0. None/-, no growth.
Supplementary Table S2. Fatty acid profiles of strain A.dayingensis TRM 4064T.
Fatty acid / A.dayingensisTRM 4064T14:0 iso / 0.7
14:0 / 0.6
15:0 iso / 16.0
15:0 anteiso / 12.2
16:0 iso / 7.0
16:1 2OH / 0.7
16:0 / 2.5
17:0 iso / 19.3
17 : 0 anteiso / 36.9
17 : 0 / 1.0
17:0 10-methyl / 0.6
18:1 cis 9 / 0.6
19 : 0 9-methyl / 2.0
Note: –, negative.Data are from this study.