An antifungal protein from Ginkgo bilobabinds actin and can trigger cell death
Ningning Gao1, Parvesh Wadhwani2, Philipp Mühlhäuser2, Qiong Liu1,MichaelRiemann1, Anne S. Ulrich2,3, Peter Nick1*
1Karlsruhe Institute of Technology (KIT), Botanical Institute and DFG-Center of Functional Nanostructures (CFN), Molecular Cell Biology, Kaiserstr. 2, 76128 Karlsruhe, Germany,
2KIT, Institute for Biological Interfaces (IBG-2), P.O.B. 3640, 76021 Karlsruhe, Germany,
3KIT, Institute of Organic Chemistry and CFN, Fritz-Haber-Weg 6, D-76131 Karlsruhe, Germany
*Corresponding author: Peter Nick;E-mail:; Tel and Fax:+4972160842142
Supplementary Materials
Table 1Primer sequences for Gateway®Cloning of full-length and truncated ginkbilobin-2
Primers / Sequences (5’- 3’)Full lengthFw
Signal peptide Fw / GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGAAGACTATGAGAATGAATTCGG
Full length Re N-terminal / GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGAAGCTCCTCTGCTCG
Full length Re C-terminal
Full length without SP Re
Domain B Re / GGGGACCACTTTGTACAAGAAAGCTGGGTCGAAGCTCCTCTGCTCGTATTG
Signal peptide Re C-terminal / GGGGACCACTTTGTACAAGAAAGCTGGGTCAGCTTCTGTAAGTATGAGCATGGC
Domain B Fw / GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCACCCACTGCCTACGG
Full length without SP Fw
Domain A1 Fw
Domain A1+A2 Fw
Domain A1+A2+A3 Fw / GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGCCAATACAGCCTTCGTC
Domain A1 Re C-terminal / GGGGACCACTTTGTACAAGAAAGCTGGGTCAAATGGGCTGCCGCTTGG
Domain A1+A2 Re C-terminal / GGGGACCACTTTGTACAAGAAAGCTGGGTCGAAGGCAGTGTTTTGCCTC
Domain A1+A2+A3 Re C-terminal / GGGGACCACTTTGTACAAGAAAGCTGGGTCTCCTCCGCTTCCTGCAC
Table 2Sequences of ginkbilobin-2 subdomain peptide conjugates
Peptide conjugates / SequencesRhodamine B- BP100- subdomain A1 (RBA1) / RhB-(KKLFKKILKYL)-(ANTAFVSSACNTQKIPSGSPF)
Rhodamine B- BP100- subdomain A2 (RBA2) / RhB-(KKLFKKILKYL)-(NLRAMLADLRQNTAF)
Rhodamine B- BP100- subdomain A3 (RBA3) / RhB-(KKLFKKILKYL)-(SGYDYKTSRAGSGG)
Rhodamine B- BP100 (RBP100) / RhB-(KKLFKKILKYL)
Table 3Antifungal activity of different ginkbilobin conjugates against Candidaalbicans
Peptide conjugates / Inhibition (MIC)Gramicidin S (control) / 128 µM
50 % Ethanol / > 256 µM
Rhodamine B / > 256 µM
Rh-BP100-A1 / 128 µM
Rh-BP100-A2 / 128 µM
Rh-BP100-A3 / 64 µM
Rh-A1 / > 256 µM
Rh-A2 / > 256 µM
Rh-A3 / > 256 µM
The Minimal Inhibitory Concentration (MIC) was determined from dose-response series. Gramicidin S was used as positive control.
Table 4 Frequency of TUNEL positive cells, 6 h after treatment with 1 µM of three different conjugates of ginkbilobin with BP100, with BP100 alone or with a mock treatment
Treatment / Frequency of TUNEL-positive cells (%) / Number of cells scoredA1-BP100 / 47 / 300
A2-BP100 / 32 / 300
A3-BP100 / 62 / 300
BP100 / 11 / 200
mock / 13 / 100
Table 5Apparent thickness of actin filaments, 1 h after treatment with 1 µM of three different conjugates of ginkbilobin with BP100 compared to a mock treatment
Treatment / Apparent thickness w [µm] / Density (#/10 µm) / nA1-BP100 / 1.34±0.23 / 1.7±0.16 / 78
A2-BP100 / 0.62±0.17 / 4.8±0.26 / 52
A3-BP100 / 1.23±0.12 / 1.2±0.06 / 83
mock / 0.45±0.19 / 6.3±0.43 / 169
Fig. 1Time course of mitotic indices (MI) for BY-2 WTandtransgenicginkbilobin-subdomainscell lines
Values represent means and standard errors from 3000 individual cells collected from three independent experimental series.
Fig.2Features of programmed cell death observed after treatment with 1 µM of a conjugate of ginkgilobin-subdomain A1 with BP100. (a) TdT-mediated dUTP-biotin nick end labelling (TUNEL) of cells that had been treated with A1 conjugate as compared to non-treated cells. Nuclei were counterstained with Höchst 22358. Size bar 100 µm. (b) Nuclear disintegration observed 6 h after treatment with A1 conjugate. Size bar 20 µm
Material 1Alignment of ginkbilobin homologuesconstructed by the ClustalW algorithm
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------MRACAYQQPGIECRLAAETSEVIAPSTLLSHGTCYC 36
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext ------
D8SW43_Selaginella_ext ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext YQSWKRLTPGQVINDGPTQTPRRRPRTGRQVRGHKSPNHAKKGCRDRREP 86
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext ------
D8SW43_Selaginella_ext ------
F2Q6K2_Ginkgo_gkl_2 ------MKT 3
A4ZDL6_Ginkgo_gkl_2 ------MKT 3
C3VHZ7_Ginkgo_gkl_2 ------MKT 3
Q40849_Picea ------MSI 3
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------M 1
D8QSH2_Selaginella ------M 1
B6U003_Zea ------MASPTRQAPAPAPAP 15
C5X4D0_Sorghum ------MAPS----- 4
I1GRN4_Brachypodium ------MAAS----- 4
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------MD 2
B9H872_Populus ------MKR 3
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------MAIKAA 6
D8RBU6_Selaginella ------MAIKAA 6
D8SW18_Selaginella ------MEVPSM 6
M0SCL2_Musa_ext ------MST------3
M0S963_Musa_ext ------MTK------3
M0TGY5_Musa_ext ------MQVQCSSQEPNHTKAKHMKE------20
M0U3Y2_Musa_ext ------MKD------3
M5XG08_Prunus_ext ------MSLTSH---- 6
B9SJ12_Ricinus_ext ------MSVTIS---- 6
S1RTU1_Theobroma_ext ------MALATK---- 6
V4UB95_Citrus_ext ------MASTQT---- 6
Q0WPN8_Arabidopsis_ext ------MPMAKLRN---- 8
D7MIF7_Arabidopsis_ext ------MAKLRN---- 6
R0F5T3_Capsella_ext ------MA--RN---- 4
V4NLK4_Thellungiella_ext ------MAKLRNSLSP 10
B9H3B4_Populus_ext ------MGRTTS---- 6
B9SYE1_Ricinus_ext ------MARTSP---- 6
K3Y7P1_Setaria_ext MSKRSAIPPLQDHPAIEATPRPSHEPATHGNNARSSVSSVMHRPCNNQP- 135
B6SZF5_Zea_ext ------MHRPRSHRL- 9
J3LZY3_Oryza_ext ------MRQR------4
Q0JBE6_Oryza_ext ------MHQL------4
Q7XU16_Oryza_ext ------MHQL------4
K7TYV4_Zea_ext ------MHLLISG--- 7
M0VQJ8_Hordeum_ext ------MPQLVT---- 6
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext ------MPCRCVGLGRGTITKE 16
D8SW43_Selaginella_ext ------
I------23-----
F2Q6K2_Ginkgo_gkl_2 -----MRMNSAFILAFALAAAMLI--LTEAANTAFVSSACNTQKIPSGSP 46
A4ZDL6_Ginkgo_gkl_2 -----MRMNSAFILAFALAAAMLI--LTEAANTAFVSSACNTQKIPSGSP 46
C3VHZ7_Ginkgo_gkl_2 -----MRMNSAFILAFALAAAMLI--LTEAANTNFVSSACNTQKIPSGSP 46
Q40849_Picea SSKFQLRSSTSLLLLVALMVVMGMDGAAAAPNTNFVSSACNTQKIPSGNP 53
D5A8I3_Picea -----MSPSSVALLAAVVIVVMGG--AAEAANTKFVSASCNTEKIARGSP 43
A9NQT1_Picea -----MSMGSFGFALAVMVLAVLVASAAGAPNTNFVSSACNTKKIPSGNP 45
Q40858_Picea -----MSMGSFGFALAVMVLAVLVASAAGAPNTNLVSSACNGNKIPSGNP 45
D9IWE0_Pinus -----MRY-SFGFTLAVMVVALVVGSAVGTPRTNLVSSACNGQKIQSGSS 44
D8R1N8_Selaginella GSLQGRKKGILVVIVSLLAVAR--LSTTAASGSELVSALCNSALYSFGDP 49
D8QSH2_Selaginella GSLQGRKNGILVVVVSLLAVAR--LSTTAASGSELVSALCNSALYSFGDP 49
B6U003_Zea APPPFLVTPIMLMLWLLLASSHTRGAMAAPPNTEALSVLCNGASYGAGDP 65
C5X4D0_Sorghum SPPPFLVLSVALWL-LLLASSHTRGAEAAPPNTEALSLLCNGASYGAGDP 53
I1GRN4_Brachypodium SRTAELIHLLLLPVALLLLFCGPHGAGAAP-STTPLSVLCNGDVYGAGDP 53
M1DNN3_Solanum -----MRVLITFLIIGLLLFSS--FVRSEP-NTKEINHICNGNVYDKGGP 42
M1DY96_Solanum ------MFLFNS--FVRSEP-NTKEINHICNGNVYDKSGP 31
K4BR56_Solanum YSVLNMRALTSFIIIGFFGFTS--FVKSEP-NMKVVSYICNGNKYDMSGS 49
B9H872_Populus TRNMGFPPKLALVFIG-FFGWC-VIATSVP-DTNVTTVLCNSGVYSKGDP 50
B9S441_Ricinus ---MGIPPKFAAVMIISFLGLCSTKVTSMP-NTQVLTVLCNAGVYSKADP 46
V4TAK1_Citrus ------MVGMLFIFC-RTVTSVP-NTTVTNVLCNSGEYTHGDP 35
I1JFB4_Glycine ---MGIPSEIAAAAIGLLCLWS--VADSVP-NTNITVILCNVGAYTSGDP 44
F6HQ79_Vitis ---MASLSNMLILVIGLLGLYG--TVRSDP-DTSVTLILCNGGGYTKGDP 44
A5AV04_Vitis ---MASLSNMLILVIGLLGLYG--TVRSDP-DISVTLILCNGGGYTKGDP 44
K4CUD1_Solanum ----MMVVKTPLTLVLILALSS-ELAKGIP-NTNLTQVLCNGAQYFKDDP 44
D8RXT4_Selaginella I--LVAIMSLAMALTIAVEARGTITSKLWRAMDVDFSGYCSDESIPTGST 54
D8RBU6_Selaginella I--LVAIMSLAMALTIAVEARGTITSKLWRAMDVDFSGYCSDESIPTGST 54
D8SW18_Selaginella ISLISKIMGVKKDVLIICAVVVAMVAAGVQGVKL-LSGLCSDESIPEGST 55
M0SCL2_Musa_ext ------FMLLLLLASSIASLTTSA---SDDYTDFVYAGCSQPKYTLGSP 43
M0S963_Musa_ext ------LLLIFLFAC-VASLSASA---SDGYTDFVYAGCSQPKCTPGSP 42
M0TGY5_Musa_ext ---MSELFPILGLLFSLISSTTMAS---FDDYTSFVYVGCSQSKYILGSP 64
M0U3Y2_Musa_ext ---THELFLLLFLLFSLRCSMAMAS---SDDYTAFVYVGCSQTKSVSSSP 47
M5XG08_Prunus_ext ----QTLVFLWVFLLATVSFLATPS---ASAINSFVFGGCSQQKYLPGSP 49
B9SJ12_Ricinus_ext ----TTAASLSFLLLTILSFLTTPA---SSSLQSFIFGGCSQLKYTSGSP 49
S1RTU1_Theobroma_ext ----TTLPFLLLLLL-VISIFITPS---NSATDSFVFGGCSQLKYTSGSP 48
I------23-----
F2Q6K2_Ginkgo_gkl_2 -----MRMNSAFILAFALAAAMLI--LTEAANTAFVSSACNTQKIPSGSP 46
V4UB95_Citrus_ext ----PSLILLLLLLLTTLSILTTPS---SSSTETFVFGGCSQQKYTPGSA 49
Q0WPN8_Arabidopsis_ext ----IIKTLSIFFF--LIAATAPSLSSATSATDTFVFGGCSQQKFSPASA 52
D7MIF7_Arabidopsis_ext ----IIKTLSFFFF--LLAATAPSLSSATSATDTFVFGGCSQQKFSPASA 50
R0F5T3_Capsella_ext ----IIKTLSFFFT--LLAATAPSLSSATSATDAFVYGGCSQQKFSPSSA 48
V4NLK4_Thellungiella_ext NN--LTKTLSFFFFS-LLAATAPSLSSATSATDTFVYGGCSQQKFSPASP 57
B9H3B4_Populus_ext ----SSLLLTFTFI---VSLSSLTCPSLSASTDAFVFGGCTQQKYVQDSP 49
B9SYE1_Ricinus_ext ----FLLILLVLFN---LSFLSIAS---SSSTDSFVFGGCTQQKYTPNSP 46
K3Y7P1_Setaria_ext ----CRGSRVAAAAVLAALM--CAAGVADAGTGTFIYAGCSPSKYQPGTP 179
B6SZF5_Zea_ext ----CR-SRVAAAVSAAIIIGLGAAGVGEAATGTFIYAGCSPSKYQPGTP 54
J3LZY3_Oryza_ext ------DRLCCSVVLAVLVALGATTVAGAATGTFIYAGCSPSKYQPGTP 47
Q0JBE6_Oryza_ext ------DRLCYAVV--VLVVIGAT-VAEAATGTFIYAGCSPSKYQPGTP 44
Q7XU16_Oryza_ext ------DRLCYAVV--VLVVIGAT-VAEAATGTFIYAGCSPSKYQPGTP 44
I------23-----
F2Q6K2_Ginkgo_gkl_2 -----MRMNSAFILAFALAAAMLI--LTEAANTAFVSSACNTQKIPSGSP 46
K7TYV4_Zea_ext ------LVAAALFLHVDAAR------GTFVYAGCSPSRYAPNTA 39
M0VQJ8_Hordeum_ext ------LLAITLLLHLHAAPTAVAQAHAATGAFIYAGCSPSKYERNAA 48
A9NPP1_Picea_ext ------METSRVFWLIAVVLLALIPTRPVESQFVYEGCSQEQYSSGSN 42
D8SCY6_Selaginella_ext ------MVFFHISLFLTLSIASNAAPVPAPIEYSCWSCGEEKFTNGSS 42
D8SH44_Selaginella_ext ------MVFFHISLFLTLSIASNAAPVPGPIEYSCWSCGEEKFTNGSS 42
C0PPW7_Picea_ext VGMAMSMKKLIMTVLVTVSLCTRMPFAAADPETSFVWQDCVNSEYRDGSA 66
D8SW43_Selaginella_ext ----MANHPLPLVLVASVFIAAFALASNSTTDIDIARPSCSDAYKITSAQ 46
---I---A2-15-----I-----14------A3--I ------B1--
F2Q6K2_Ginkgo_gkl_2 FNRNLRAMLADLRQNTAFSGY----DYKTSRAGSGG------APTAYGRA 86
A4ZDL6_Ginkgo_gkl_2 FNRNLRAMLADLRQNTAFSGY----DYKTSRAGSGG------APTAYGRA 86
C3VHZ7_Ginkgo_gkl_2 FNRNLRAMLADLKQNTAFSGY----DYKTSRAGSGG------APTAYGRA 86
Q40849_Picea FFTNLRAMLADLKQNTAFSGF----DYKTSRAGSGG------APTAYGRA 93
D5A8I3_Picea FFNNLRDAIEDLRQNTAYIGY----DYKTSHVGSGG------APTAYGRA 83
A9NQT1_Picea FFNNLGALLVNLEKNTAFSGY----DYKASRAGSGG------APTAYGRG 85
Q40858_Picea FFNNLGALLVDLEKNTAFSGF----DYKASRAGSGG------APTAYGRG 85
D9IWE0_Pinus FYSTLGSLLVDLEGNTASSGY----DYKASRAGS------PTAYGRA 81
D8R1N8_Selaginella FGKNLGLVLDDLLGTTPGRDF---KDYTAISPRPS------RFVYGHA 88
D8QSH2_Selaginella FGKNLGLVLDDLLGTTPRRDF---KDYTAISPRPF------RFVYGHA 88
B6U003_Zea FATSLAFVLSELVSATPVRAG---HDFYDISPYPA------AFAYGHA 104
C5X4D0_Sorghum FATSLAYVLSELVSATPARAGG--HDFYDISPYPA------AFAYGHA 93
I1GRN4_Brachypodium FAASLAYVLSDLLARAPSSSPSP-RDAYSISPWPN------AFAYGHA 94
M1DNN3_Solanum FALSLAYVVEALQNVTP-NQG---YDYYITSPYPND------ALAYGHA 81
M1DY96_Solanum FALSLAYVVEALQNVTP-NQG---YDYYITSPYPNE------ALAYGHA 70
K4BR56_Solanum FANSLAYVLEALQNETPISQG---YNYYVTSPYPND------ALAYGHA 89
B9H872_Populus FGTSLAYVLAEIEAVTPTSKN---YDYFNISPYPN------AVAYGHA 89
B9S441_Ricinus FAISLAYVVEELEKKTAESKN---YDYFNISPYPN------AFAYGHA 85
V4TAK1_Citrus FAISLAYVLADLETVTSASKD---YDYYNISPYPN------AFAYGHA 74
I1JFB4_Glycine FAVSLSYVVRELETETPTRKN---YDYYNISPYPN------AFAYGHA 83
F6HQ79_Vitis FATSLTYVVEDLEAKTPKRKG---YNYYNISPYPN------SFAYGYA 83
A5AV04_Vitis FATSLTYVVEDLEAKTPKRKG---YNYYNISPYPN------SFAYGYA 83
K4CUD1_Solanum FAISLAYVLAELVSTTPSRQG---YDYHNISPYPN------AFAYGHA 83
D8RXT4_Selaginella YWTSLNQTLTDLIQNTAYANN---MTYSVKNGGD------DVSAYGQA 93
D8RBU6_Selaginella YWTSLNQTLTDLIQNTAYANN---MTYSVKNGGD------DVSAYGQA 93
D8SW18_Selaginella YWNSLNATLADLVQNTPTAAN---MTYSTNKGVEG------DVPAYGQA 95
M0SCL2_Musa_ext YQLNVDSVLTSLANAAAFSS------YANFTSSA-----AGGSFPAYGLF 82
M0S963_Musa_ext YQFNVESLLASLANAAAYSS------YANFTSTA-----AASSSPAYGLF 81
M0TGY5_Musa_ext YQFNVDSLLTSVANAATFSS------YNNFTSSA-----ASGSSPAYGLF 103
M0U3Y2_Musa_ext YQSNVDSLLTSLANAAALSS------YDSFTSSA-----ASATSPAYGLF 86
M5XG08_Prunus_ext YESKVNSLLTSLVNSAMFTT------YNNFTIPG-----SSSQDTVYGLF 88
B9SJ12_Ricinus_ext YESNVNSLLTSLVNSATLTN------YNNFTIQSP----SSSQDTLYGLF 89
S1RTU1_Theobroma_ext YESNVNSILTSLVNSAMFTS------YNNFTMPG-----STSQDTVYGLF 87
---I---A2-15-----I-----14------A3--I ------B1--
F2Q6K2_Ginkgo_gkl_2 FNRNLRAMLADLRQNTAFSGY----DYKTSRAGSGG------APTAYGRA 86
V4UB95_Citrus_ext YENSVNSMLTSLVNSAMFSS------YNNFTIQG------QQTLYGLF 85
Q0WPN8_Arabidopsis_ext YESNLNSLLTSLVNSATYSS------YNNFTIMG-----SSSSDTARGLF 91
D7MIF7_Arabidopsis_ext YESNLNSLLTSLVNSATYSS------YNNFTIMG-----SSSSDTAHGLF 89
R0F5T3_Capsella_ext YESNLNSLLTSLVNSATYSS------YNNFTILG-----SSSSDTARGLF 87
V4NLK4_Thellungiella_ext YESNLNSLLTSLVNSATYSS------YNNFTVMG-----SSSSDTARGLF 96
B9H3B4_Populus_ext FESNLNSLLTSLVNSATYSS------YNNYTIMG-----SSPQDVVYGLY 88
B9SYE1_Ricinus_ext YESNINSLLTSLVNSATYTS------YNNFTIMG-----STPQDVVYGLF 85
K3Y7P1_Setaria_ext FKANLESLLTSISSAALNGG------YNTFTAGA---NGTGGAPAAYGLY 220
B6SZF5_Zea_ext FEANLESLLASMAGAAPKAG------YSSFTAGA---GGVGNGTTAYGLY 95
J3LZY3_Oryza_ext FEGNLNSLLASFANAAPNGG------YNSFTAGS---NGTGDGVAAYGLY 88
Q0JBE6_Oryza_ext FEGNLNSLLASIANAAPNGG------YNSFTAGS---NGTGDGAAAYGLY 85
Q7XU16_Oryza_ext FEGNLNSLLASIANAAPNGG------YNSFTAGS---NGTGDGAAAYGLY 85
---I---A2-15-----I-----14------A3--I ------B1--
F2Q6K2_Ginkgo_gkl_2 FNRNLRAMLADLRQNTAFSGY----DYKTSRAGSGG------APTAYGRA 86
K7TYV4_Zea_ext FESNLNSLLSSMAPTASSGAT-----YSTFTAPG-AGLAEAESPAAYGLY 83
M0VQJ8_Hordeum_ext FQSNLDSLLTSIASTASSGAA-----YNSFTAGGVVGQPEAARTAAYGLY 93
A9NPP1_Picea_ext YENNLNSLLGILLRQASSADY-----YNASVGSG------GDAIYGLY 79
D8SCY6_Selaginella_ext YSTALDNLLATLITNAPVTGF-----FQSSVQSA------ELTLYGLL 79
D8SH44_Selaginella_ext YSTALDNLLATLITNAPVTGF-----FQSSVQSA------ELTLYGLL 79
C0PPW7_Picea_ext FQRNLNNVLESLVRKVNPSGFN----ISSIVVGGKN-----SNSSVYGFV 107
D8SW43_Selaginella_ext MGRNLLTVLATLQDQGPAAGG-----YKSTSFGSMS------DTVYGFI 84
: : . *
------30------I------B2------
F2Q6K2_Ginkgo_gkl_2 TCKQS------ISQSDCTACLSNLVNRIFSICN--NAIGARVQLVD-CFI 127
A4ZDL6_Ginkgo_gkl_2 TCKQS------ISQSDCTACLSNLVNRIFSICN--NAIGARVQLVD-CFI 127
C3VHZ7_Ginkgo_gkl_2 TCKQS------ISQSDCTACLSNLVNRIFSICN--NAIGARVQLVD-CFI 127
Q40849_Picea ICKSS------ISQSDCSACLSNLVGRIWGICS--NAIGARVQLTD-CFI 134
D5A8I3_Picea ICKQS------ISQSNCTSCLSNVATRIYGICG--NAIGARVQLTD-CYM 124
A9NQT1_Picea VCKQS------ISQSDCTACLTNLGGRIWGICK--NAIGARVQLTD-CFI 126
Q40858_Picea VCKQS------ISQSDCTACLTNLGGRIWGISK--NAIGARVQLTD-CFI 126
D9IWE0_Pinus VCNQG------LSQSDCTACLKNLGGRIWNICG--YAIGARVQLTD-CFI 122
D8R1N8_Selaginella KCRHA------LSAGECGVCLKGVAAALADQCG--NAVGGRAMFVD-CFM 129
D8QSH2_Selaginella KCRHA------LSAGECGVCLKGVAAALADQCG--NAVGGRAMFVD-CFM 129
B6U003_Zea ACRAALS------AADCAACLRSAVSQMDASCG--HSVGARAVLVD-CSV 145
C5X4D0_Sorghum ACRAALS------APDCATCLRSAVSQMDASCG--HSVGARAVLVD-CSV 134
I1GRN4_Brachypodium ACGARVP-T----HAACAACLGSAIAQINSSCGA-AAVGARAVLVD-CRV 137
M1DNN3_Solanum TCNSI------IEFSDCGLCLSSAKSFLLRTCD--GSIGGQVEFVD-CSM 122
M1DY96_Solanum TCNSI------IEFSDCGLCLSSAKTFLLSTCD--GNIGGQVEFVD-CSM 111
K4BR56_Solanum TCSSS------LINSDCAICANYAKSYLMTACD--RGIGAQIELID-CSM 130
B9H872_Populus ACNQN------LTSSDCSSCLGTAKTAMFASCQ--SRIGARSVLHD-CTI 130
B9S441_Ricinus ACNQN------LTSSDCTACLGAAKTAMFSSCP--SRIGARSVLHD-CTI 126
V4TAK1_Citrus ACNKN------LTSPDCTSCLGAAKTAMLGSCP--SRIGSRSVLHD-CKI 115
I1JFB4_Glycine ACNLN------LTATDCKTCLGASKTAMFNGCP--KRVGARSVLHD-CTI 124
F6HQ79_Vitis SCSQN------LTSKDCSTCLSSAKTAMFGTCD--NRIGARAVLHD-CQI 124
A5AV04_Vitis SCSQN------LTSKDCSTCLSSAKTAMFGTCD--NRIGARAVLHD-CQI 124
K4CUD1_Solanum ACNNNTT-TTSLTKQDCKTCLDSAKDNLLTSCD--AQIGGRVLLVD-CTM 129
D8RXT4_Selaginella QCF-----NMVGNPGMCKSCVEDLVRRAWRECG--DAIAGMLMLKDKCTI 136
D8RBU6_Selaginella QCF-----NMVGNPGMCKSCVEDLVRRAWRECG--DAIAAMLMLKDKCTI 136
D8SW18_Selaginella QCLRNATTNVLPSQDSCRGCIEDIIAKAWLDCV--DAIAVDVKLNDDCTL 143
M0SCL2_Musa_ext QCRGD------LPFSDCDSCVRSGLSRLSAFCP--SAAGAAVQLRG-CFL 123
M0S963_Musa_ext QCRGD------LPFSDCHSCVRSALSQLSSLCP--SAAGAAIQFRG-CFL 122
M0TGY5_Musa_ext QCRVD------LSVADCASCVRSALDQLSSLCP--SASGAGVQLKG-CFV 144
M0U3Y2_Musa_ext QCRTD------LPNSVCASCVRSALVQLSSLCP--SSTGATVQLKG-CFF 127
M5XG08_Prunus_ext QCRGD------LSNNDCAQCVARSVSQLGNLCL--NSCGGALQLEG-CFI 129
B9SJ12_Ricinus_ext QCRGD------LSNGDCARCVAAAVSQLGTLCL--DSCGGALQLDG-CFV 130
S1RTU1_Theobroma_ext QCRGD------LNNGDCARCVAKAVSQLGTLCL--DSTGGALQLEG-CFV 128
: : . *
------30------I------B2------
F2Q6K2_Ginkgo_gkl_2 TCKQS------ISQSDCTACLSNLVNRIFSICN--NAIGARVQLVD-CFI 127
V4UB95_Citrus_ext QCRGD------LSTADCGRCVAHAVSQLGTLCL--DSCGGVLQLDG-CFV 126
Q0WPN8_Arabidopsis_ext QCRGD------LSMPDCATCVARAVSQVGPLCP--FTCGGALQLAG-CYI 132
D7MIF7_Arabidopsis_ext QCRGD------LSMPDCATCVVRAVSQVGPLCP--FTCGGALQLAG-CYI 130
R0F5T3_Capsella_ext QCRGD------LSMPDCATCVARAVSQVGPLCP--LTCGGALQLAG-CYV 128
V4NLK4_Thellungiella_ext QCRGD------LSMPDCATCVARAVSQVGPLCP--YTCGGALQLAG-CYI 137
B9H3B4_Populus_ext QCRGD------LSMPDCATCVARAVSQLGVLCA--QTCGGALQLQG-CYV 129
B9SYE1_Ricinus_ext QCRGD------LSMPDCATCVARAVSQLGSLCS--QTCGGAVQLQG-CYV 126
K3Y7P1_Setaria_ext QCRGD------LDGGDCAACVRDAVGQLGQVCP--AAYAASLQLEG-CYV 261
B6SZF5_Zea_ext QCRGD------LAGADCAACVRDALGQLGQVCA--AAYAASLQLEG-CYV 136
J3LZY3_Oryza_ext QCRGD------LGNDDCSACVRDAVAQLNEVCP--SAYAASLQLEG-CYV 129
Q0JBE6_Oryza_ext QCRGD------LGNADCAACVRDAVGQLNEVCA--AAYAASLQLEG-CYV 126
Q7XU16_Oryza_ext QCRGD------LGNADCAACVRDAVGQLNEVCA--AAYAASLQLEG-CYV 126
------30------I------B2------
F2Q6K2_Ginkgo_gkl_2 TCKQS------ISQSDCTACLSNLVNRIFSICN--NAIGARVQLVD-CFI 127
K7TYV4_Zea_ext QCRGG------LRPGECVACVRDTVARIGAVCA--GAAAASLQSDG-CYV 124
M0VQJ8_Hordeum_ext QCRGD------LSRGQCVACVRETVARLGAVCA--NSYAASLQVDG-CYV 134
A9NPP1_Picea_ext DCRGD------LGSDDCANCIKNAMSQISNVCL--RTRGARIQLDG-CFM 120
D8SCY6_Selaginella_ext QCRQD------MSRDSCDQCARDIGQAVKSRCS--SSLGAKIQLYG-CFL 120
D8SH44_Selaginella_ext QCRQD------MSRDSCDQCARDIGQAVKSRCS--SSLGAKIQLYG-CFL 120
C0PPW7_Picea_ext QCRGD------LDSSDCEQCASMAKTILVQGCH---NISGFVQLDG-CFL 147
D8SW43_Selaginella_ext QCRGD------ISAQECSSCARSAVQILNSTCVRNSTKGGRIHPGN-CFL 127
Q10ME0_Oryza QCRGD------VSASDCKACLVEAANYTLSFCH---YASDSRMWYDYCFM 127
* * * . . . * .
--26--I
F2Q6K2_Ginkgo_gkl_2 QYEQRSF------134
A4ZDL6_Ginkgo_gkl_2 QYEQRSF------134
C3VHZ7_Ginkgo_gkl_2 QYEQRSF------134
Q40849_Picea QYEQHSF------141
D5A8I3_Picea QYEQRSF------131
A9NQT1_Picea RYEQYSI------133
Q40858_Picea RYEQYSI------133
D9IWE0_Pinus RYEQYSF------129
D8R1N8_Selaginella RYEAYPFGDEEPAWSIS------146
D8QSH2_Selaginella RYEAYPFGDEEPAWSIS------146
B6U003_Zea RYEQYAFVD------154
C5X4D0_Sorghum RYEQYAFVD------143
I1GRN4_Brachypodium RYEQYAFVD------146
M1DNN3_Solanum RYEQY-FS------129
M1DY96_Solanum RYEQYSFS------119
K4BR56_Solanum RYEQYSFT------138
B9H872_Populus RYEQYPFSD------139
B9S441_Ricinus RYEQYPFND------135
V4TAK1_Citrus RYEQYPFED------124
I1JFB4_Glycine RYEQYPFDD------133
F6HQ79_Vitis RYEQYPFDD------133
A5AV04_Vitis RYEQYPFDD------133
K4CUD1_Solanum RYEQYPFDD------138
D8RXT4_Selaginella RYQDSPL------143
D8RBU6_Selaginella RYQDSPL------143
D8SW18_Selaginella RYQDSPLLPDVIQGERDLP------162
M0SCL2_Musa_ext RYGNDSFLGKPDTSVLYKKCGPAAA-GGYNSDQLGMRDAALA------DL 166
M0S963_Musa_ext RYGNDSFLGKADTSVLYKKCGPPVG-GGYNSDMLGMRDAALA------DL 165
M0TGY5_Musa_ext RYGNDSFLGEPDTTLLYKKCGSVVPGGGYNSDLLGMRDAALS------GL 188
M0U3Y2_Musa_ext RFGDEPFLGKPDTTLLYNKCGSVAP-DEHSSDLLVMRDTALS------GL 170
M5XG08_Prunus_ext KYDNSTFLGVEDKTVVIKKCGQSIG---FDSDVLTRRDAVLG------YL 170
B9SJ12_Ricinus_ext KYDNMSFLGVEDKTVVLKKCGPSIG---YDSNALTRRDAILG------YL 171
S1RTU1_Theobroma_ext KYDNITFLGVEDKTVVVKKCGPLIS---SYSDALTRRDAVLG------YL 169
--26--I
F2Q6K2_Ginkgo_gkl_2 QYEQRSF------134
V4UB95_Citrus_ext KYDNISFLGVEDKTVVVKKCGPLIG---YDSDASTRRDAVLG------YL 167
Q0WPN8_Arabidopsis_ext KYDNISFLGQEDKTVVLKKCGSSEG---YNTDGISRRDAVLT------EL 173
D7MIF7_Arabidopsis_ext KYDNISFLGQEDKTVVLKKCGPSEG---YNTDGISRRDAVLT------EL 171
R0F5T3_Capsella_ext KYDNISFLGQEDKTVVLKKCGPSEG---YNTDGISRRDAVLT------EL 169
V4NLK4_Thellungiella_ext KYDNISFLGQEDKTVVLKKCGPSEG---YNTDGISRRDAVLT------GL 178
B9H3B4_Populus_ext KYDNTTFLGVEDKTVVLKKCGPSVG---YDTDAMNLRDAVLG------GL 170
B9SYE1_Ricinus_ext KYDNATFLGVEDKTIVLKKCGPSIG---YDTDAMSVRDAVFA------GL 167
K3Y7P1_Setaria_ext RYDSSNFVGAPDTAMVYRKCSTSSS---SDGDFLRSRDAVLG--DLQAVG 306
B6SZF5_Zea_ext RYDGTDFLGTPDAAMVYRKCSTSSS---SDGEFLRDRDAVLAG-DLLQGG 182
J3LZY3_Oryza_ext RYDSSNFVGQPDNAMVYRKCSTSTN---SDADFLKNRDAVLA---ALQGG 173
Q0JBE6_Oryza_ext RYDSSNFVGQPDNAMVYRKCSTSTS---GDGDFLKNRDAVLA---ALQGG 170
Q7XU16_Oryza_ext RYDSSNFVGQPDNAMVYRKCSTSTS---GDGDFLKNRDAVLA---ALQGG 170
--26--I
F2Q6K2_Ginkgo_gkl_2 QYEQRSF------134
K7TYV4_Zea_ext RYGGA------SAAYHSCGAGTS---GDPWFLRSRDAVLSELQGVDAT 163
M0VQJ8_Hordeum_ext RY------DAS 139
A9NPP1_Picea_ext HYDRINFFGQTDNSFIYKTCSETLS---SDYNFNNHLNTIMG--SLMTAS 165
D8SCY6_Selaginella_ext RYENHPFFSVLDESLLCLNCSSIAS----SPGYDDNLKSALA------TL 160
D8SH44_Selaginella_ext RYENHPFFSVLDESLLCLNCSSIAS----SPGYDDNLKSALA------TL 160
C0PPW7_Picea_ext RYENRSFYN--DNVLTPNTPTNYMCNLGNSSRPQQFTNAVSALLSNVTAK 195
D8SW43_Selaginella_ext RYEDHFFSSILDKDTNQGNCSTSIP-ASSLAIFQSNSRTALEFLKAKVPR 176
Q10ME0_Oryza RYKNANFIGQVDTRVDASVRIRKEE------152
::
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext TSGAA-GGSYRVGAA-----GYVQAMAQCVGDQSAKECDDCVAAAVAHLR 210
M0S963_Musa_ext TSDSA-RGSYRIGAA-----GYVQAVAQCVGDQSAKHCEDCLAAAVAELR 209
M0TGY5_Musa_ext SGG---GGSYRVGAG-----GHVRALAQCVGDQSVKQCSDCVAAAVAQLK 230
M0U3Y2_Musa_ext ASG---GGNYRLGTG-----GSVRGLAQCVGDQSPNDCGGCVAAAVAQLK 212
M5XG08_Prunus_ext GTGDGTYRPYRVSGS-----GNVQGVAQCVGDLSPSECQDCLSEAIAQLK 215
B9SJ12_Ricinus_ext GASDGSYKPYRVAGS-----GDVYGVAQCVQDLSASECQDCLSDAVERLK 216
S1RTU1_Theobroma_ext AASDGTYKPFRVGGS-----GDIQGVAQCVGDLSPSECQDCLSEATGRLK 214
V4UB95_Citrus_ext GSSDGSYKPYRVGGS-----GDVTGVAQCVGDLSASECQDCLSDAIGRLK 212
Q0WPN8_Arabidopsis_ext VNGGG---YFRAGGS-----GDVQGMGQCVGDLTVSECQDCLGTAIGRLK 215
D7MIF7_Arabidopsis_ext VNGGG---YFRAGGS-----GDVQGMGQCVGDLTVSECQDCLGTAIGRLK 213
R0F5T3_Capsella_ext VNGGG---YFRAGGS-----GDVQGMGQCVGDLTVSECQDCLGTAIGKLK 211
V4NLK4_Thellungiella_ext LSGGG---YFRAGGS-----GDVQGMGQCVGDLTVSECQDCVGTAIGRLK 220
B9H3B4_Populus_ext ARTGG---AYRVGGS-----GDVQGVAQCIGDLSTGECQDCLSEAIMRLK 212
B9SYE1_Ricinus_ext VRAGG---PYRIGGS-----GEVQGVAQCVGDLSLGECQDCLSEAIERLK 209
K3Y7P1_Setaria_ext AG------GYKVASS-----GSVRGLAQCLGDLAAADCTACLAQAAGQLK 345
B6SZF5_Zea_ext AANNGSSNGYKAGSS-----GSVQGVAQCLGDLAAPDCAACLAQAVGQLK 227
J3LZY3_Oryza_ext LAN-----GYKVSSS-----GNVQGVSQCLGDLAAADCTTCLAQAVGQLK 213
Q0JBE6_Oryza_ext LAN-----GYKVSSS-----GNVQGVSQCLGDLAAGDCTTCLAQAVGQLK 210
Q7XU16_Oryza_ext LAN-----GYKVSSS-----GNVQGVSQCLGDLAAGDCTTCLAQAVGQLK 210
K7TYV4_Zea_ext AASGSGSGGYKVSAS-----GPVRGAAQCLGGIAAGDCAACLAQAAAQLR 208
M0VQJ8_Hordeum_ext DFVG------R------ADNTVAYR 152
A9NPP1_Picea_ext SGDNNGFRLTTAGDSST---GFVHGLAQCEGDLSVSDCNNCMNAAVQKLK 212
D8SCY6_Selaginella_ext SRRGSSFTATVGSGK-----SQVFVLGECRGDLSSQQCDSCVRVAMRNMT 205
D8SH44_Selaginella_ext SRRGSSFTATVGSGK-----SQVFVLGECRGDLSSQQCDSCVRVAMRNMT 205
C0PPW7_Picea_ext AAGSPKLFATDSVAGPSSSRGNIYSLAQCWRYLSRTDCGSCLTFAQETLL 245
D8SW43_Selaginella_ext NARKFVAAVAGIGSA-----VPAYALGQCIPDLSAADCASCLAAAENAIA 221
Q10ME0_Oryza ------NMDNPKTFQKVVAKTMDKYPRRWRPRGVPHWE 184
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext AVCGFAVAGDAY-LGKCYAKYWSNGVYTT-SKTDHDPNQLKSQRKTTERM 258
M0S963_Musa_ext AACGFAVAGDAY-LGKCFARYWSNGVYT--SKTDHD------242
M0TGY5_Musa_ext DGCGVANAGDAY-LGKCYVSYWSDGVYT--SRHYHG------263
M0U3Y2_Musa_ext DICGVADAGDAY-LGKCYVRYWSDAADT--SGHHHG------245
M5XG08_Prunus_ext SGCGPSAWGDMF-LAKCYARYSQGGYHTNGGHDYHNDDDD------254
B9SJ12_Ricinus_ext TDCGPAASGDMY-LAKCYVRFSERGAHSHGGNDDHNDD------253
S1RTU1_Theobroma_ext TECGAVKWGDMY-LAKCYARYSEGGDHSHGEK------245
V4UB95_Citrus_ext TDCGAAKWGDMF-LAKCYARYSEGGDHSHGGKG------244
Q0WPN8_Arabidopsis_ext NDCGTAVFGDMF-LAKCYARYSTDGAQHY--AKSHN------248
D7MIF7_Arabidopsis_ext NDCGTAVFGDMF-LAKCYARYSTDGAQHY--AKSHS------246
R0F5T3_Capsella_ext SDCGTAVFGDMF-LAKCYARYSTDGAQHY--AKSHN------244
V4NLK4_Thellungiella_ext NDCGTAVFGDMF-LAKCYARYSTDGGQYN--AKSHK------253
B9H3B4_Populus_ext SDCGTADYGDMF-LAKCYARYTTGGPHVY--TKSHSGKHT------249
B9SYE1_Ricinus_ext SDCGTAVYGDMF-LAKCYARYSTGGAQVY--TKSHN------242
K3Y7P1_Setaria_ext GTCGNALAADVY-LAQCYVRYWADGYYFRPTQDYSQ------380
B6SZF5_Zea_ext GTCGTALAADVY-LAQCYVRYWADGYYFRPTQDYSQ------262
J3LZY3_Oryza_ext GTCGTSLAADVY-LAQCYVRYWANGFYFRPSQDYSQ------248
Q0JBE6_Oryza_ext GTCGTSLAADVY-LAQCYVRYWANGFYFRPSQDYSQ------245
Q7XU16_Oryza_ext GTCGTSLAADVY-LAQCYVRYWANGFYFRPSQACSA------245
K7TYV4_Zea_ext GTCGAALAADVY-LVQCSVRYWTDGANQ---LRSSQ------240
M0VQJ8_Hordeum_ext ------KCSS------SSS------159
A9NPP1_Picea_ext SVCGTAVSGQVY-LSKCYVRYAQDGFYRDSSGTGNSSS------249
D8SCY6_Selaginella_ext DRCIGNTSGSIL-LNSCYTRF------225
D8SH44_Selaginella_ext DRCIGNTSGSIL-LNSCYTRF------225
C0PPW7_Picea_ext FGCHMGAPGAQFGSDKCYLRYEVYEFFTTSVLSPPPGESP------285
D8SW43_Selaginella_ext STCRGGGGGVCY-YSSCTLFFQRSKFFQGIPENATVLGNG------260
Q10ME0_Oryza G------185
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext GGTGLSPLSLSLYKSGDEAGKTLAIIIGLMAGVALIIVFLSFLRRAGHDG 308
M0S963_Musa_ext ------DEAGKTLAIIIGLIAGVALIIVLLSFLRKAGNYG 276
M0TGY5_Musa_ext ------KRLYVNWRWSAVAFFCLLS------282
M0U3Y2_Musa_ext ------NRPSGGRRWSVIAFLCLLSVATLGCV-- 271
M5XG08_Prunus_ext ------DDDDDELEKTLAILIGVIAGVALLVVFLSYFRKYLCEE 292
B9SJ12_Ricinus_ext ------EIEKTLAILIGLIAGVALLIVFLSFLRK-VCEK 285
S1RTU1_Theobroma_ext ------VGGAVENTWLVA------257
V4UB95_Citrus_ext ------KRFAFRHLVWWSFLVFGLINLVLVL--- 269
Q0WPN8_Arabidopsis_ext ------YKTNYGGEKTFAIIIGLLAAVVLLIIFLLFLRGVCSRG 286
D7MIF7_Arabidopsis_ext ------YKTNYGGERTFAIIIGLLAGVVLLIIFLLFLRGVCSRG 284
R0F5T3_Capsella_ext ------YKANYGGERTFAIIIGLLAAVVLLIIFLLFLRGVCSRG 282
V4NLK4_Thellungiella_ext ------YKTNYGGERTFAIIIGLLAAVVLLIIFLLFLRGVCSRG 291
B9H3B4_Populus_ext ------DKSTSDGEKTFAIIIGLLAGVALLIIFLSFLRKVFGGN 287
B9SYE1_Ricinus_ext ------DKSTNEGEKTFAIIIGLLAGVALIIIFLTFIRKVFEGS 280
K3Y7P1_Setaria_ext ------DDIGRTLAIVVGIMAGLALFVVFISFLRKTCN-- 412
B6SZF5_Zea_ext ------DDVGRTVAIIVGILAGLAIFVVFISFLRKAC--- 293
J3LZY3_Oryza_ext ------DDVGRTVAIIVGILAGLAIIVVFISFLRKSCKLA 282
Q0JBE6_Oryza_ext ------DDVGRTVAIIVGILAGLAILVVFISFLRKSC--- 276
Q7XU16_Oryza_ext ------KDE--SNHLIEGSHA--TDKPKFSLLAIDYCL-- 273
K7TYV4_Zea_ext ------GDPRPYMA------248
M0VQJ8_Hordeum_ext ------EDAG------163
A9NPP1_Picea_ext ------GNGDDAGKTVAIIIGLLAGVALIVVFISFLKQVFKRG 286
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext ------PSSSEGKRPRVLGISLSVVAFTIVLIAAIGFWKWNFCSR 324
D8SW43_Selaginella_ext ------TLPSNSKAKKTLLLAIAIPIAGVLALLLLVIGFFISKRSKN 301
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ISPNTFLSSLPFHS------CPYHPLLFFWFICR------336
M0S963_Musa_ext L------LSF------280
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext EKCGK------297
B9SJ12_Ricinus_ext GKGGK------290
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext GK------288
D7MIF7_Arabidopsis_ext GDFYFLHIFII------295
R0F5T3_Capsella_ext GGGK------286
V4NLK4_Thellungiella_ext GK------293
B9H3B4_Populus_ext GKIPTLLAFNFFFF------LTANIASHACHACR------315
B9SYE1_Ricinus_ext GK------282
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext LTPEGVSVQLALCA------R------297
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext HNKGNYSTCKTLIS------VFLSFLIQVFKHIQHL------316
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext AKRREEIYRTGGDEGDSVLMPTIANPELIFKFDILRESTSNFKAENKLGE 374
D8SW43_Selaginella_ext VSKPPEELDELQVT------LHRGPVTFSYDLLRRATEGFSDDHKLGE 343
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext GGFGSVFKGVLPDGREVAVKRLFMGTRQADAEFLNEANLISRVQHRNLVK 424
D8SW43_Selaginella_ext GGFGAVYKGTFSDGTEIAIKKLSVASKQWEQQFLNEVKVIGSLHSNRLLP 393
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext LLGCSVEVSERLLVYEYLQNSSLDKILFDPTKRHLLDWKKRSEIILGTAR 474
D8SW43_Selaginella_ext NLLHRKLVSSSNNEQEKTLVQAIHIALMCTQASPQDRPSMSHVVAMLSGH 443
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext GLAYLHEESDVRVIHRDIKASNILLDDKHRPKIADFGLARFFAEDQSHVS 524
D8SW43_Selaginella_ext SNVNVPLSATFDIRAMNPRFYSSSSNSAASDPKNKRQEKSQTFTDPEPR- 492
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
I1MZF0_Glycine_ext ------
K7MPT1_Glycine_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext TRVAGTLGYMAPEYALRGQLTEKADVFSFGVLVLEIISGRKNQSSTEDME 574
D8SW43_Selaginella_ext ------
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext FLIEGTWRLYKANRGLEIMDPALKDSYSWEDGIRAIKIGLLCTQAAAALR 624
D8SW43_Selaginella_ext ------
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext PSMFRVVSMLTSEREHLPSPTRPAFIDLDAAGAAQQLKRGRIIDPERTSS 674
D8SW43_Selaginella_ext ------
Q10ME0_Oryza ------
F2Q6K2_Ginkgo_gkl_2 ------
A4ZDL6_Ginkgo_gkl_2 ------
C3VHZ7_Ginkgo_gkl_2 ------
Q40849_Picea ------
D5A8I3_Picea ------
A9NQT1_Picea ------
Q40858_Picea ------
D9IWE0_Pinus ------
D8R1N8_Selaginella ------
D8QSH2_Selaginella ------
B6U003_Zea ------
C5X4D0_Sorghum ------
I1GRN4_Brachypodium ------
M1DNN3_Solanum ------
M1DY96_Solanum ------
K4BR56_Solanum ------
B9H872_Populus ------
B9S441_Ricinus ------
V4TAK1_Citrus ------
I1JFB4_Glycine ------
F6HQ79_Vitis ------
A5AV04_Vitis ------
K4CUD1_Solanum ------
D8RXT4_Selaginella ------
D8RBU6_Selaginella ------
D8SW18_Selaginella ------
M0SCL2_Musa_ext ------
M0S963_Musa_ext ------
M0TGY5_Musa_ext ------
M0U3Y2_Musa_ext ------
M5XG08_Prunus_ext ------
B9SJ12_Ricinus_ext ------
S1RTU1_Theobroma_ext ------
V4UB95_Citrus_ext ------
Q0WPN8_Arabidopsis_ext ------
D7MIF7_Arabidopsis_ext ------
R0F5T3_Capsella_ext ------
V4NLK4_Thellungiella_ext ------
B9H3B4_Populus_ext ------
B9SYE1_Ricinus_ext ------
K3Y7P1_Setaria_ext ------
B6SZF5_Zea_ext ------
J3LZY3_Oryza_ext ------
Q0JBE6_Oryza_ext ------
Q7XU16_Oryza_ext ------
K7TYV4_Zea_ext ------
M0VQJ8_Hordeum_ext ------
A9NPP1_Picea_ext ------
D8SCY6_Selaginella_ext ------
D8SH44_Selaginella_ext ------
C0PPW7_Picea_ext SAATSSTTPSETHSAAADPNASSILEPR 702
D8SW43_Selaginella_ext ------
Q10ME0_Oryza ------
1