Figure 2: ED-1+ Cells (Recruited Macrophages) in Rat Liver After Single-Dose TAA-Administration

Figure 1: Immunodetection of neutrophil elastase (NE, a marker for neutrophil granulocytes) in sections of rat livers after single-dose TAA-administration. Sections were stained with an antibody directed against NE followed by peroxidase staining (A-J). A and B show NE+ cells (neutrophil granulocytes) at 0 hours, C and D at 6 hours, E and F at 12 hours, G and H at 24 hours, I and J at 48 hours after single-dose TAA-treatment. The left panel shows NE+ cells around the portal area and the right panel shows NE+ cells around the central vene area. Results show representative pictures of six animals and six slides per time point. Original magnification, x100. P: portal area; CV: centrilobular vene.

Figure 2: ED-1+ cells (recruited macrophages) in rat liver after single-dose TAA-administration. A and B show ED-1+ cells at 6 hours, C and D at 12 hours, E and F at24 hours as well as G and H at 48 hours after single-dose TAA-treatment. The left panel shows NE+ cells around the portal area and the right panel shows NE+ cells around the central vene area. The images are representative pictures of six animals and six slides per time point. Original magnification, x100. P: portal area; CV: centrilobular vene.

Figure 3: ED-2+ cells (resident macrophages) in rat liver after single-dose TAA-administration. A and B show ED-2+ cells at 6 hours, C and D at 12 hours,E and F at 24 hours as well as G and H at 48 hoursafter single-dose TAA-treatment. The left panel shows NE+ cells around the portal area and the right panel shows NE+ cells around the central vene area. Results show representative pictures of six animals and six slides per time point. Original magnification, x100, B original magnification x200. P: portal area; CV: centrilobular vene.

Figure 4: Double staining of liver sections with antibodies directed against CD-11b/c (green, a marker for granulocytes and macrophages), ED-1 (red, a marker for resident macrophages), -SMA (red, a marker for myofibroblasts) and CK-19 (red, a marker for cytokeratin 19) followed by fluorescence immunodetection in sections of rat liver after single-dose TAA-administration A-C: liver sections at 0 hours; D-F: liver sections at 12 hours; G-I: liver sections at 48 hours. From left to right: CD-11b/c+ and ED-1+ cells (recruited macrophages); CD-11b/c+ and -SMA+ cells; and CD-11b/c+ and CK-19+ cells (marker for bile duct cells). Results show representative pictures of six animals and six slides per time point. Original magnification, x200. P: portal area; CV: centrilobular vene.