Fig. S1 Phylogenetic Tree of S-Rbps. the Phylogenetic Tree Was Made by Clastalw2 Phylogeny

Fig. S1

Fig. S1 Phylogenetic tree of S-RBPs. The phylogenetic tree was made by ClastalW2 Phylogeny using default parameters.

Fig. S2

Fig. S2 Salt stress response of S-RBP11 RNAi transgenic plants. (a) Schematic map of RNAi vector for the suppression of S-RBP11 and expression analysis of S-RBP11 in T1 RNAi lines. GAPc was used as an internal control. (b) and (c) Responses of WT and S-RBP11 RNAi T2 seedlings to 0, 130, or 140 mM NaCl. In (c), error bars represent standard deviation (n = 24 plants).

Fig. S3

Fig. S3 Expression analysis of S-RBP11 under abiotic stresses using semi-quantitative RT-PCR. (a) Expression of S-RBP11 under 300 mM NaCl treatment for 0, 1, 2, 4, or 8 h. (b) Expression of S-RBP11 under cold treatment for 0, 1, 6, 12, or 24 h. (c) Expression of S-RBP11 under 10 μM treatment for 0, 1, 2, 4, or 8 h. In (a) to (c), GAPc was used as an internal control. Similar results were obtained from at least two biological replicates, with one shown here.

Table S1 Primers used in this study

Gene / Forward / Reverse / Purpose
35S enhancer copy 1 / 5′-TATCTCGAGGATCCCCAACATGGTGGAGC-3′ / 5′-CGCGTCGACTAGATATCACATCAATCCAC-3′ / Cloning
35S enhancer copy 2 / 5′-TATGTCGACGATCCCCAACATGGTGGAGC-3′ / 5′-CGCAAGCTTTAGATATCACATCAATCCAC-3′ / Cloning
35S enhancer copy 3 / 5′-TATAAGCTTGATCCCCAACATGGTGGAGC-3′ / 5′-CGCGAATTCTAGATATCACATCAATCCAC-3′ / Coning
35S enhancer copy 4 / 5′-TATGAATTCGATCCCCAACATGGTGGAGC-3′ / 5′-CGCCTGCAGTAGATACACATCAATCCAC-3′ / Coning
LB1 / 5′-ATGGTCATAGCTGTTTCCTGTGTGAAATTG-3′ / TAIL-PCR
LB2 / 5′-AACCTGTCGTGCCAGCTGCATTAATGAATC-3′ / TAIL-PCR
AD1 / 5′-NGTCGASWGANAWGAA-3′ / TAIL-PCR
AD2 / 5′-TGWGNAGSANCASAGA-3′ / TAIL-PCR
AD3 / 5′-AGWGNAGWANCAWAGG-3′ / TAIL-PCR
AD4 / 5′-WGTGNAGWANCANAGA-3′ / TAIL-PCR
LB-seq / 5′-CATTAATGAATCGGCCAACG-3′ / Sequencing
S-RBP11 / 5′-ATCGTTTTGCACTACAGAGC-3′ / 5′-CACGAAACCGAAACCTTTTG-3′ / Quantitative
RT-PCR
RD29A / 5′-CCTGAAGTGATCGATGCACC-3′ / 5′-CAGTGGGTTTGGTGTAATCG-3′ / Quantitative
RT-PCR
COR15A / 5′-AACGCAAGAAGTCGTTGATC-3′ / 5′-CGTTGAGGTCATCGAGGATG-3′ / Quantitative
RT-PCR
GAPc / 5'-GTGTCCCAACCGTTGATGTC-3' / 5'-TCCCTTGAGTTTGCCTTCGG-3' / Quantitative
RT-PCR
S-RBP11 / 5'-AAATTTTAATGGCGGCGCTC-3' / 5'-TGCGTAGTCCACAAATATCG-3' / Semi-quantitative RT-PCR
At5g06190 / 5′-TACAGCTCGCGATCCAGATC-3′ / 5′-ATTATTCCTCTAGCCCTCAG-3′ / Semi-quantitative RT-PCR
GAPc / 5'-CACTTGAAGGGTGGTGCCAAG-3' / 5'-CCTGTTGTCGCCAACGAAGTC-3' / Semi-quantitative RT-PCR
Entire S-RBP11 / 5'-ATATCTAGAATGGCGGCGCTCGCA AGGAT-3' / 5'-TATCCCGGGGTCGCTTTTCGATGTTTCGG-3' / Cloning
Truncated
S-RBP11 / 5'-CCGTCTAGAATGTCCGCCTCATGTTTCTTC-3' / 5'-TATCCCGGGGTCGCTTTTCGATGTTTCGG-3' / Cloning
sGFP / 5'-ATCCTGCAGATGGTGAGCAAGGGCGAGGAG-3' / 5'-ATCGTCGACCTTGTACAGCTCGTCCATGCC-3' / Cloning
S-RBP11 RNAi / 5′-GAAGATCCAGCGAAAAGATGGAC
GGCTGTTCAAGAGACAGCCGTCCA
TCTTTTCGCTGGATCTTTTTTTG-3′ / 5′-GATCCAAAAAAACGGACAGCAATTAA
ACGGCCGGACGTCTCTTGAACGTCCGG
CCGTTTAATTGCTGTCCGTTCTGCA-3′ / Cloning

Table S2 List of small RNA-binding proteins in Arabidopsis

S-RBP / Protein code / AGI code / aa length / Domain
S-RBP1 / CAB78428, NP_193122 / At4g13860 / 126 / One RRM
S-RBP2 / BAB09544, NP_200183 / At5g53720 / 100 / One RRM
S-RBP3 / AAC61284, NP_179093 / At2g14870 / 101 / One RRM
S-RBP4 / CAB88326, NP_190188 / At3g46020 / 102 / One RRM
S-RBP5 / CAB78306, NP_193001 / At4g12640 / 823 / Two RRMs and SPOC domain
S-RBP6 / AAC36547 / alcohol dehydrogenase, partial (Fragaria viridis)
S-RBP7 / AAG51218 , NP_973958 / At1g33470 / 245 / One RRM
S-RBP8 / AAD20390, NP_179762 / At2g21690 / 117 / One RRM
S-RBP9 / AAC23648, NP_181287 / At2g37510 / 142 / One RRM
S-RBP10 / AAF21210, NP_187457 / At3g08000 / 143 / One RRM
S-RBP11 / BAB09686, NP_196239 / At5g06210 / 146 / One RRM
S-RBP12 / BAB09337, NP_200269 / At5g54580 / 156 / One RRM
S-RBP13 / BAB08354, NP_200794 / At5g59860 / 157 / One RRM
S-RBP14 / AAB88654, NP_850366 / At2g42240 / 292 / Two RRMs
S-RBP15 / BAB09540, NP_200179 / At5g53680 / 169 / One RRM