KUZNETSOV / CURRICULUM VITAE
NAME: / Sergey G. Kuznetsov
DATE & PLACE OF BIRTH: / November 9, 1972, Dzhambul, Kasakhstan, USSR
NATIONALITY: / Russian
CONTACT INFORMATION / Sergey G. Kuznetsov,
Mailing address:
Institute for Molecular Medicine Finland,
P.O. Box 20, FI-00014 University of Helsinki, Finland
Street address:
FIMM, Biomedicum Helsinki 2U (E303b),
Tukholmankatu 8, 00290 Helsinki,
Finland
Tel.+358-9-191-25750
GSM +358-50-4151173
E-mail:

EDUCATION:

Dates / Discipline / Degree / Institution
09.2002 – 09.2008 / Post-doctoral
training / Mouse Cancer Genetics Program, NCI, NIH, Frederick, MD, USA
02.1998 – 02.2002 / Cell Biology / Dr. rer. nat. (Ph.D.),
Thesis title: “Role of apoptosis and epithelial cells in Hydra
spermatogenesis and histocompatibility reactions” / University of Kiel, Germany
08. 1995 – 12. 1997 / Cell Biology / Master of Science (M.Sc.) / St.-Petersburg State University, Russia
08. 1990 – 06. 1995 / Cell Biology, Histology / Bachelor of Science (B.S.) / St.-Petersburg State University, Russia

EMPLOYMENT HISTORY AND RESEARCH EXPERIENCE:

13.10.2008 – present / FIMM-EMBLInternational group leaderat the Institute for Molecular Medicine Finland (FIMM), University of Helsinki, Finland.
21.09.2002 – 21.09.2008 / Postdoctoral training in the laboratory of Dr. Shyam Sharan in the Mouse Cancer Genetics Program at National Cancer Institute, NIH, in Frederick, MD, USA.
02.1998 - 02.2002 / Postgraduate training in the laboratory of Prof. Thomas C.G. Bosch at Zoological Institutes of University of Jena and University of Kiel, Germany
10.1992 – 12.1997 / Undergraduate training under supervision of Prof. Vladimir B. Klimovich at Hybridoma Technology Laboratory, Central Roentgeno-Radiological Research Institute, St.-Petersburg, Russia.

AWARDS & DISTINCTIONS:

2008selected as an EMBL-style group leader at Univ. Helsinki - FIMM

2005, 2006, 2007cash awards by NIH

02.1998 – 10.2000 PhD Scholarship (Nr. 02-32/97) from Gottlieb Daimler- and Karl Benz foundation (Germany)

10.1995 – 08.1996Exchange Fellowship from Munich University, Germany

COMPETITIVE FUNDING:

Funded period / Amount of Funding / Funding agency / Project description
2010 / €20.000,00 / Cancer Society of Finland / High throughput screen for BRCA1-cooperating genes in breast cancer cell lines
2011 -2012 / €50.000,00 / Cancer Society of Finland / High throughput screen for BRCA1-cooperating genes in breast cancer cell lines
2010-2012 / €57.000,00 / Biocenter Finland / Training grant from the LentiGEMM consortium for developing a pooled in vivo RNAi screen in mouse mammary glands
2011-2014 / €363.000,00 / Academy of Finland / Academy research fellow post

SUPERVISORY EXPERIENCE:

MSc, 01.10.2011 – 11.09.2013: Sharif Iqbal, University of Helsinki,Faculty of Agriculture and Forestry: The role of SMARCD1 in breast cancer.

PhD, 01.02.2009 –current: Manuela Tumiati, University of Helsinki, Faculty of Medicine, HBGS: Investigation of mechanisms of Rad51c-mediated cancer initiation and progression.

PhD, 10.11.2009 –current: Yuexi Gu, University of Helsinki, Faculty of Biological and Environmental Sciences, GSBM: Identification of new therapeutic targets for BRCA1-associated breast cancers.

PhD, 25.11.2011 – current: Daria Bulanova, University of Helsinki, Faculty of Biological and Environmental Sciences, GSBM: Identification of novel tumor suppressors involved in basal-like breast cancers.

SPOKEN LANGUAGES: Russian (native), German (fluent), English (fluent), Spanish (basics), Finnish (basics)

TEACHING EXPERIENCE:

  • Organizing and teaching a course on theory and practice of functional genomics for Helsinki Biomedical Graduate Programme, University of Helsinki, October 3 – November 14, 2011
  • Organizing and teaching a lecture and practical course on Mutagenesis and DNA repair in affiliation with Helsinki Biomedical Graduate Programme, University of Helsinki, October 30 – November 6, 2009

INVITED ORAL PRESENTATIONS AT INTERNATIONAL CONFERENCES

  1. Rad51c is a p53-dependent tumour suppressor in mice. Annual meeting of the Genomic Instability in Cancer consortium (GENICA). Athens, Greece, May 16-19, 2009.
  1. Integrative RNAi screening approaches using cell spot microarrays: functional review of DNA damage response in cancer cells. PhD Training School “High-Throughput Screens in Genome Integrity and Cancer”, University of Oxford, UK, 24-27 May 2010

PUBLICATIONS

  1. Sokolenko AP, Bulanova DR, Iyevleva AG, Aleksakhina SN, Preobrazhenskaya EV, Ivantsov AO, Kuligina ES, Mitiushkina NV, Suspitsin EN, Yanus GA, Zaitseva OA, Yatsuk OS, Togo AV, Kota P, Dixon MJ, Larionov AA, Kuznetsov SG, Imyanitov EN. (2013) High prevalence of GPRC5A germ-line mutations in BRCA1-mutant breast cancer patients. IJC, 2013 (in press)
  2. Kuligina ESh, Sokolenko AP, Mitiushkina NV, Abysheva SN, Preobrazhenskaya EV, Gorodnova TV, Yanus GA, Togo AV, Cherdyntseva NV, Bekhtereva SA, Dixon JM, Larionov AA, Kuznetsov SG, Imyanitov EN. Value of bilateral breast cancer for identification of rare recessive at-risk alleles: evidence for the role of homozygous GEN1 c.2515_2519delAAGTT mutation.Fam Cancer. 2013; 12(1): 129-32.
  3. Sokolenko AP, Iyevleva AG, Preobrazhenskaya EV, Mitiushkina NV, Abysheva SN, Suspitsin EN, Kuligina ES, Gorodnova TV, Pfeifer W, Togo AV, Turkevich EA, Ivantsov AO, Voskresenskiy DV, Dolmatov GD, Bit-Sava EM, Matsko DE, Semiglazov VF, Fichtner I, Larionov AA, Kuznetsov SG, Antoniou AC, Imyanitov EN. High prevalence and breast cancer predisposing role of the BLM c.1642 C>T (Q548X) mutation in Russia.Int J Cancer. 2012; 130(12): 2867-73.
  4. Biswas K, Das R, Alter BP, Kuznetsov SG, Stauffer S, North SL, Burkett S, Brody LC, Meyer S, Byrd RA, Sharan SK. A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay.Blood. 2011;118(9):2430-42
  5. Kuznetsov S, Chang S, Sharan S. Functional analysis of human BRCA2 variants using a mouse embryonic stem cell-based assay. Methods Mol Biol. 2010;653:259-280
  6. Kuznetsov S, Haines D, Martin B, Sharan S. Loss of Rad51c leads to embryonic lethality and modulation of Trp53-dependent tumorigenesis in mice. Cancer Res. 2009;69:863-872
  7. Li L, Biswas K, Habib L, Kuznetsov S, Hamel N, Kirchhoff T, Wong N, Armel S, Chong G, Narod S, Claes K, Offit K, Robson M, Stauffer S, Sharan S, Foulkes W. Functional redundancy of exon 12 of brca2 revealed by a comprehensive analysis of the c.6853a>g (p.I2285v) variant. Hum Mutat. 2009;30:1543-1550
  8. Sharan S, Thomason L, Kuznetsov S, Court D. Recombineering: A homologous recombination-based method of genetic engineering. Nat Protoc. 2009;4:206-223
  9. Kuznetsov S, Liu P, Sharan S. Mouse embryonic stem cell-based functional assay to evaluate mutations in BRCA2. Nat Med. 2008;14:875-881
  10. Philip S, Swaminathan S, Kuznetsov S, Kanugula S, Biswas K, Chang S, Loktionova N, Haines D, Kaldis P, Pegg A, Sharan S. Degradation of BRCA2 in alkyltransferase-mediated DNA repair and its clinical implications. Cancer Res. 2008;68:9973-9981
  11. Zhang N, Kuznetsov S, Sharan S, Li K, Rao P, Pati D. A handcuff model for the cohesin complex. J Cell Biol. 2008;183:1019-1031
  12. Kuznetsov S, Pellegrini M, Shuda K, Fernandez-Capetillo O, Liu Y, Martin B, Burkett S, Southon E, Pati D, Tessarollo L, West S, Donovan P, Nussenzweig A, Sharan S. Rad51c deficiency in mice results in early prophase i arrest in males and sister chromatid separation at metaphase ii in females. J Cell Biol. 2007;176:581-592
  13. Sharan S, Kuznetsov S. Resolving rad51c function in late stages of homologous recombination. Cell Div. 2007;2:15
  14. Khalturin K, Kuznetsov S, Bosch T. PCR fluorescence differential display. Methods Mol Biol. 2003;226:237-244
  15. Kuznetsov S, Bosch T. Self/nonself recognition in cnidaria: Contact to allogeneic tissue does not result in elimination of nonself cells in hydra vulgaris. Zoology (Jena). 2003;106:109-116
  16. Kuznetsov S, Anton-Erxleben F, Bosch T. Epithelial interactions in Hydra: Apoptosis in interspecies grafts is induced by detachment from the extracellular matrix. J Exp Biol. 2002;205:3809-3817
  17. Kuznetsov S, Lyanguzowa M, Bosch T. Role of epithelial cells and programmed cell death in Hydra spermatogenesis. Zoology (Jena). 2001;104:25-31
  18. Samoĭlovich M, Kuznetsov S, Pavlova M, Klimovich V. [monoclonal antibodies to hydra vulgaris and hydra oligactis antigens]. Zh Evol Biokhim Fiziol. 2001;37:262-269
  19. Venturelli C, Kuznetsov S, Salgado L, Bosch T. An IQGAP-related gene is activated during tentacle formation in the simple metazoan Hydra. Dev Genes Evol. 2000;210:458-463

Manuscripts in PROGRESS:

  1. Munne PM, Tumiati M, Gu Y, Koopal S, Uusivirta S, Sawicki J, Kuznetsov SG. TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli.Sci Rep, (under revision).

1 | Page