Electronic Supplementary Material s71

Electronic Supplementary Material

Assessment of Infections with Microsporidia and Cryptosporidium spp. in Fecal Samples from Wild Primate Populations from Cameroon and Democratic Republic of Congo

Christelle Butel · Steve Ahuka Mundeke · Pascal Drakulovski · Donika Krasteva · Eitel Mpoudi Ngole · Michele Mallié · Eric Delaporte · Martine Peeters · Sabrina Locatelli

Table SI Sample collection information, SIV serology results, PCR results for Microsporidium and Cryptospordium spp., and microsatellite genotypes with assigned individuals

Collection information / Serology / Genetic profile (consensus multilocus genotype)
Date (mmaa) / Locationa / Samples field number / Speciesb / Assigned
individual / INNO-LIA HIV-1/2c / Microsporidium ssp. / Cryptosporidium ssp. / D18S536 / D4S243 / D10S676 / D9S922 / D2S1326 / D2S1333 / D4S1627 / Sexd
04/05 / MB-CM / CR 0749 / P.t.t. / ID-1 / + / — / — / 154/170 / 196/228 / 181/189 / –/ – / 255/263 / 314/322 / 228/228 / – / –
04/05 / MB-CM / CR 0750 / P.t.t. / ID-2 / + / — / — / 166/170 / 200/228 / 177/177 / – / – / 243/251 / 322/322 / 228/236 / – / –
04/05 / MB-CM / CR 0751 / P.t.t. / ID-2 / + / — / — / 166/170 / 200/228 / 177/177 / – / – / 243/251 / 322/322 / 228/236 / – / –
04/05 / MB-CM / CR 0752 / P.t.t. / ID-2 / + / — / — / 166/170 / 200/228 / 177/177 / – / – / 243/251 / 322/322 / 228/236 / – / –
04/05 / MB-CM / CR 0753 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0754 / P.t.t. / ID-3 / – / E. intestinalis / — / 150/158 / 224/228 / 177/189 / 294/298 / 235/263 / 314/322 / 220/228 / M
04/05 / MB-CM / CR 0755 / P.t.t. / ID-4 / + / — / — / 166/170 / 200/228 / 177/177 / – / – / 243/251 / 322/322 / 228/236 / – / –
04/05 / MB-CM / CR 0756 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0757 / P.t.t. / ID-3 / – / E. intestinalis / — / 150/158 / 224/228 / 177/189 / 294/298 / 235/263 / 314/322 / 220/228 / M
04/05 / MB-CM / CR 0758 / P.t.t. / ID-4 / + / — / — / 166/170 / 200/228 / 177/177 / – / – / 243/251 / 322/322 / 228/236 / – / –
04/05 / MB-CM / CR 0759 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0760 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0761 / P.t.t. / ID-5 / – / E. hellem / — / 162/162 / 204/216 / 185/189 / – / – / 251/271 / 286/302 / – / – / – / –
04/05 / MB-CM / CR 0762 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0763 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0764 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0765 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0766 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0767 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0768 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0769 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0770 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0771 / P.t.t. / ID-6 / + / — / — / 146/154 / 204/248 / 177/189 / 304/308 / 251/267 / 326/330 / 232/236 / – / –
04/05 / MB-CM / CR 0772 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0773 / P.t.t. / ID-7 / + / — / — / 154/154 / 228/228 / 177/181 / – / – / 251/267 / 306/326 / 216/232 / – / –
04/05 / MB-CM / CR 0774 / P.t.t. / ID-8 / + / — / C. cuniculus / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0775 / P.t.t. / ID-8 / + / — / — / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0776 / P.t.t. / ID-9 / + / — / — / 166/174 / 228/232 / 177/185 / – / – / 243/267 / 318/326 / 232/236 / – / –
04/05 / MB-CM / CR 0777 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0778 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0780 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0781 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0782 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0783 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0784 / P.t.t. / ID-10 / + / — / — / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0785 / P.t.t. / ID-10 / + / — / — / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0786 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0787 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0788 / P.t.t. / ID-10 / + / — / — / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0790 / P.t.t. / ID-11 / + / — / — / 150/154 / 248/248 / 185/189 / 296/304 / 239/247 / 298/314 / 224/228 / – / –
04/05 / MB-CM / CR 0791 / P.t.t. / ID-10 / + / — / — / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0792 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0801 / P.t.t. / ID-12 / + / E. hellem / — / 142/154 / 228/232 / 177/185 / – / – / 231/251 / 322/326 / 224/228 / – / –
04/05 / MB-CM / CR 0802 / P.t.t. / ID-13 / + / — / — / 158/170 / 228/228 / 177/177 / – / – / 231/255 / 318/326 / 212/236 / – / –
04/05 / MB-CM / CR 0803 / P.t.t. / ID-14 / + / — / — / 162/174 / 228/228 / 177/177 / – / – / 231/255 / 330/334 / 228/232 / – / –
04/05 / MB-CM / CR 0804 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0805 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0806 / P.t.t. / + / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0807 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0808 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0809 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0810 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0811 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0812 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0882 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0886 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0888 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0889 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0890 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0892 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/05 / MB-CM / CR 0893 / P.t.t. / ID-15 / – / — / — / 154/158 / 228/232 / 177/185 / 300/308 / 231/231 / –/326 / 236/236 / M
04/05 / MB-CM / CR 0902 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2327 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2328 / P.t.t. / – / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2329 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2330 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2331 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2332 / P.t.t. / ID-16 / – / E. hellem / — / 158/162 / 228/228 / 181/185 / 296/308 / 255/259 / 306/326 / 224/232 / F
04/07 / MB-CM / CR 2333 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2334 / P.t.t. / ID-17 / + / — / — / 146/154 / 224/224 / 177/189 / 296/304 / – / – / – / – / – / – / – / –
04/07 / MB-CM / CR 2335 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2336 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2337 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2338 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2339 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
04/07 / MB-CM / CR 2340 / P.t.t. / ID-18 / + / — / C. ubiquitum / 158/170 / 244/244 / 169/177 / 304/316 / 231/255 / 330/334 / 232/232 / F
03/10 / MB-CM / CR 5131 / P.t.t. / ID-19 / + / — / — / 162/162 / 220/224 / 173/177 / 300/300 / 239/263 / 310/322 / 228/232 / M
03/10 / MB-CM / CR 5132 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5133 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5134 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5135 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5136 / P.t.t. / ID-20 / + / — / — / 162/162 / 224/224 / 173/177 / 300/300 / 235/263 / 310/310 / – / – / F
03/10 / MB-CM / CR 5137 / P.t.t. / ID-20 / + / — / — / 162/162 / 224/224 / 177/177 / 300/300 / 239/263 / 310/310 / 228/232 / F
03/10 / MB-CM / CR 5138 / P.t.t. / ID-20 / + / — / — / 162/162 / 224/224 / 177/177 / 300/300 / 239/263 / 310/310 / – / – / F
03/10 / MB-CM / CR 5139 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5140 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5141 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5143 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5144 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5145 / P.t.t. / ID-21 / + / — / — / 162/162 / 204/248 / 177/189 / 292/296 / 259/267 / 326/330 / 232/236 / F
03/10 / MB-CM / CR 5146 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5147 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5148 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5151 / P.t.t. / ID-22 / – / — / — / 162/162 / 224/248 / 169/173 / 292/300 / 239/263 / 306/330 / 228/232 / F
03/10 / MB-CM / CR 5152 / P.t.t. / ID-22 / – / — / — / 162/162 / 224/248 / 169/173 / 292/300 / 239/263 / 306/330 / 228/232 / F
03/10 / MB-CM / CR 5153 / P.t.t. / ID-22 / – / — / — / 162/162 / 224/248 / 169/173 / 292/300 / 239/263 / 306/330 / 228/232 / F
03/10 / MB-CM / CR 5154 / P.t.t. / ID-22 / – / — / — / 162/162 / 224/248 / 169/173 / 292/300 / 239/263 / 306/330 / 228/232 / F
03/10 / MB-CM / CR 5155 / P.t.t. / ID-22 / – / — / — / 162/162 / 224/248 / 169/173 / 292/300 / 239/263 / 306/330 / 228/232 / F
03/10 / MB-CM / CR 5156 / P.t.t. / ID-22 / + / — / — / 162/162 / 224/248 / 169/173 / 292/300 / 239/263 / 306/330 / 228/232 / F
03/10 / MB-CM / CR 5157 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5158 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5159 / P.t.t. / ID-23 / + / — / — / 162/162 / 220/224 / 173/177 / 296/308 / 255/267 / 306/322 / 228/236 / M
03/10 / MB-CM / CR 5160 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5161 / P.t.t. / ID-23 / + / — / — / 162/162 / 200/224 / 173/177 / 296/308 / 255/267 / 306/322 / 228/236 / M
03/10 / MB-CM / CR 5163 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5165 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5166 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5167 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5168 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5169 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5170 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5171 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
03/10 / MB-CM / CR 5454 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6232 / P.t.t. / ID-24 / + / — / — / 166/170 / 224/224 / 177/177 / 300/300 / 239/263 / 310/322 / 228/232 / F
11/10 / MB-CM / CR 6233 / P.t.t. / ID-24 / + / — / — / 166/170 / 224/224 / 177/177 / 300/300 / 239/263 / 310/322 / 228/232 / F
11/10 / MB-CM / CR 6234 / P.t.t. / ID-24 / + / — / — / 166/170 / 224/224 / 177/177 / 300/300 / 239/263 / 310/322 / 228/232 / F
11/10 / MB-CM / CR 6235 / P.t.t. / ID-24 / + / — / — / 166/170 / 224/224 / 177/177 / 300/300 / 239/263 / 310/322 / 228/232 / F
11/10 / MB-CM / CR 6236 / P.t.t. / ID-24 / + / — / — / 166/170 / 224/224 / 177/177 / 300/300 / 239/263 / 310/322 / 228/232 / F
11/10 / MB-CM / CR 6237 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6238 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6239 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6240 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6243 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6244 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6249 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6250 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND
11/10 / MB-CM / CR 6254 / P.t.t. / + / — / — / ND / ND / ND / ND / ND / ND / ND / ND
02/11 / MB-CM / CR 6377 / P.t.t. / – / — / — / ND / ND / ND / ND / ND / ND / ND / ND